Click here to switch to the map view.

The map label for this gene is dusA [H]

Identifier: 222111076

GI number: 222111076

Start: 2003621

End: 2004676

Strand: Direct

Name: dusA [H]

Synonym: Dtpsy_1883

Alternate gene names: 222111076

Gene position: 2003621-2004676 (Clockwise)

Preceding gene: 222111074

Following gene: 222111078

Centisome position: 52.77

GC content: 69.41

Gene sequence:

>1056_bases
ATGCCCTCCTCCATCGCCCACAGTCCCTGGCGTATGTCCGTTGCGCCGATGATGGACTGGACCGACAGGCACTGCCGCTA
CCTGCACCGCCTGCTGTCGCGCCACACGCTGCTCTACACCGAGATGGTGACCACCGGTGCGCTGGCGCACGGCGACGTGC
AGCGCCATCTGCGCTTTGACGCGGCCGAGCACCCCGTGGCGCTGCAGTTGGGCGGCAGCGAGCCGGCCGACCTCGCGCAC
TGCGCCCGTCTGGGCGAGCAGTGGGGCTATGACGAGATCAATCTCAACTGCGGCTGCCCCAGCGAGCGTGTGCAGCGCGG
CGCTTTCGGCGCCTGCCTGATGAACGAGCCGCAACTGGTCGCCGACTGCGTGAAAGCCATGGTGGACGTGGTTCAGGTCC
CGGTCACCGTCAAGCACCGCATCGGGATCGACCAGAACGAAGACTACGGTTTCGTGCGCGACTTCGTGGGCGTGGTGGCC
GACGCGGGCTGCCGCGTGTTCATCGTGCATGCGCGCAATGCGTGGCTGAAGGGTCTGTCGCCCAAGGAGAACCGCGAGAT
TCCCCCGCTGCGCTACGACGTCGCGGCCCGGCTGAAAGCCGACTTCCCGCAACTGACCATCGCCATCAACGGGGGCTTTC
AGACCGACGCCGCGGTGCTGGAGGAGCTGGCCCGGGTGGATGGCGTGATGGTGGGCCGCGAGGCCTACCACAACCCCTGG
TGGCTGGCGCGCTGGGACGAGCAGTTCTTTGGCGCCGCGCCCGCCGCGCCCACGCGCGAGCAGGTCGAGGAAGACATGGT
GGCCTACATGGAGCGCGAAGCCACCGCGCACGGCACGCACTGGTATGCGGTGGCGCGCCACATGCTGGGCCTGCGCAACG
GCCTGCCCGGCGCGCGACGCTGGCGCCAAGTGTGGAGCGACCACCGCTTGAAACACCTGCCCGCGCGCGAAGTGATGGCC
CTGGCGCGCACCAAGCCGGGCGCCACCGCGCCCGATGCACTGCGCGCAAGCGCCGTGCAAGCCGGCGCCCTGCCAACGGA
GCCGCTCAGCCTTTGA

Upstream 100 bases:

>100_bases
AATCCCTTGCAACTGTGGCGCAGCGCCGGACCTGGGACAATACTGGCCCCCGCCCCGCTCCCTTGTCCCCCAGACCGCCC
CGTCCTTCACCGTGCACGCT

Downstream 100 bases:

>100_bases
GGCCCGCCAGCCAGTCCCGCAGCGGCTGCGGCAGCCCGAACAGATAGCCCTGCAGGCCATGGCAGTGGTGGGCGATGAGG
AAGTCCGCCTGCTGCCGCGT

Product: tRNA-dihydrouridine synthase a

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MPSSIAHSPWRMSVAPMMDWTDRHCRYLHRLLSRHTLLYTEMVTTGALAHGDVQRHLRFDAAEHPVALQLGGSEPADLAH
CARLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMVDVVQVPVTVKHRIGIDQNEDYGFVRDFVGVVA
DAGCRVFIVHARNAWLKGLSPKENREIPPLRYDVAARLKADFPQLTIAINGGFQTDAAVLEELARVDGVMVGREAYHNPW
WLARWDEQFFGAAPAAPTREQVEEDMVAYMEREATAHGTHWYAVARHMLGLRNGLPGARRWRQVWSDHRLKHLPAREVMA
LARTKPGATAPDALRASAVQAGALPTEPLSL

Sequences:

>Translated_351_residues
MPSSIAHSPWRMSVAPMMDWTDRHCRYLHRLLSRHTLLYTEMVTTGALAHGDVQRHLRFDAAEHPVALQLGGSEPADLAH
CARLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMVDVVQVPVTVKHRIGIDQNEDYGFVRDFVGVVA
DAGCRVFIVHARNAWLKGLSPKENREIPPLRYDVAARLKADFPQLTIAINGGFQTDAAVLEELARVDGVMVGREAYHNPW
WLARWDEQFFGAAPAAPTREQVEEDMVAYMEREATAHGTHWYAVARHMLGLRNGLPGARRWRQVWSDHRLKHLPAREVMA
LARTKPGATAPDALRASAVQAGALPTEPLSL
>Mature_350_residues
PSSIAHSPWRMSVAPMMDWTDRHCRYLHRLLSRHTLLYTEMVTTGALAHGDVQRHLRFDAAEHPVALQLGGSEPADLAHC
ARLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMVDVVQVPVTVKHRIGIDQNEDYGFVRDFVGVVAD
AGCRVFIVHARNAWLKGLSPKENREIPPLRYDVAARLKADFPQLTIAINGGFQTDAAVLEELARVDGVMVGREAYHNPWW
LARWDEQFFGAAPAAPTREQVEEDMVAYMEREATAHGTHWYAVARHMLGLRNGLPGARRWRQVWSDHRLKHLPAREVMAL
ARTKPGATAPDALRASAVQAGALPTEPLSL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=254, Percent_Identity=28.740157480315, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI145693211, Length=310, Percent_Identity=58.7096774193548, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789660, Length=235, Percent_Identity=27.2340425531915, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788462, Length=173, Percent_Identity=32.3699421965318, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=233, Percent_Identity=25.7510729613734, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 39126; Mature: 38995

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSIAHSPWRMSVAPMMDWTDRHCRYLHRLLSRHTLLYTEMVTTGALAHGDVQRHLRFD
CCCCCCCCCCCEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AAEHPVALQLGGSEPADLAHCARLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLV
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCHHHCCCCHHH
ADCVKAMVDVVQVPVTVKHRIGIDQNEDYGFVRDFVGVVADAGCRVFIVHARNAWLKGLS
HHHHHHHHHHHHCCEEEHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHCCCC
PKENREIPPLRYDVAARLKADFPQLTIAINGGFQTDAAVLEELARVDGVMVGREAYHNPW
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEECHHHHCCCC
WLARWDEQFFGAAPAAPTREQVEEDMVAYMEREATAHGTHWYAVARHMLGLRNGLPGARR
EEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHH
WRQVWSDHRLKHLPAREVMALARTKPGATAPDALRASAVQAGALPTEPLSL
HHHHHHHCHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PSSIAHSPWRMSVAPMMDWTDRHCRYLHRLLSRHTLLYTEMVTTGALAHGDVQRHLRFD
CCCCCCCCCCEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AAEHPVALQLGGSEPADLAHCARLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLV
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCHHHCCCCHHH
ADCVKAMVDVVQVPVTVKHRIGIDQNEDYGFVRDFVGVVADAGCRVFIVHARNAWLKGLS
HHHHHHHHHHHHCCEEEHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHCCCC
PKENREIPPLRYDVAARLKADFPQLTIAINGGFQTDAAVLEELARVDGVMVGREAYHNPW
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEECHHHHCCCC
WLARWDEQFFGAAPAAPTREQVEEDMVAYMEREATAHGTHWYAVARHMLGLRNGLPGARR
EEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHH
WRQVWSDHRLKHLPAREVMALARTKPGATAPDALRASAVQAGALPTEPLSL
HHHHHHHCHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]