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The map label for this gene is mutB [H]

Identifier: 222111042

GI number: 222111042

Start: 1968258

End: 1970420

Strand: Direct

Name: mutB [H]

Synonym: Dtpsy_1849

Alternate gene names: 222111042

Gene position: 1968258-1970420 (Clockwise)

Preceding gene: 222111039

Following gene: 222111043

Centisome position: 51.84

GC content: 64.77

Gene sequence:

>2163_bases
ATGAGCAGCCATCCTGATCCCATCTTCCAGTCCGCCGACCTTGCCGCCTGGGCCAAGGCCGCGGCCAAGTCCGCGCCCGG
CGGCGACGTGAATGCCCTGAACTGGGTGACGCCCGACGGCATCAGCGTGAAGCCCCTGTACACGGCGCAAGACACGCAGG
ACCTGCCCTACGCCAACACGCTGCCGGGCTTCGAGCCCTACCTGCGCGGCCCGCAGGCCACCATGTACGCCGTGCGCCCC
TGGACCATCCGCCAGTACGCGGGCTTTTCCACGGCCGAAGAATCCAACGCCTTCTACCGCAAAGCGCTGGCCGCGGGCGG
GCAGGGTGTGTCGGTGGCCTTTGACCTGGCCACGCACCGCGGCTACGACTCCGACCACCCGCGCGTGACGGGCGACGTGG
GCAAGGCGGGCGTCGCCATCGACAGCGTGGAGGACATGAAGATCCTGTTCGACGGCATTCCGCTGGACAAGGTGTCTGTT
TCCATGACCATGAACGGCGCCGTGCTGCCCGTGCTGGCCGGCTACGTGGTGGCAGCCGAGGAGCAGGGCGTGAGCCAGGA
CAAGCTGGCCGGGACCATCCAGAACGACATCCTCAAGGAGTTCATGGTCCGCAACACCTACATCTATCCGCCCAAGCCGT
CGATGCGCATCATCGGCGACATCATCGAGTACACGAGCAAGAACATGCCCAAGTTCAACTCGATCTCGATCTCGGGCTAC
CACATGCAGGAAGCCGGCGCCAACCAGGCGCTGGAACTGGCCTTCACCCTGGCCGACGGCAAGGAATACGTGAAGACGGC
CATCGCCAAGGGCATGGACGTGGACGATTTCGCCGGGCGCCTGTCGTTCTTCTGGGCGATCGGCATGAACTTCTACCTGG
AGATCGCCAAGATGCGCGCCGCGCGCCTGCTGTGGTGCCGCATCATGAAGGGCTTCAACGCCAAAAACCCCAAGAGCCTG
ATGCTGCGCACGCACTGCCAGACCTCGGGCTGGTCGCTCACCGAGCAGGACCCGTACAACAACGTGGTGCGCACCACCAT
CGAGGCCATGGCGGCCGTGTTCGGCGGCACGCAAAGCCTGCACACCAACGCGCTGGACGAAGCCATCGCGCTGCCCACCG
AATTCAGCGCACGCATCGCGCGCAACACGCAGCTCATCATCCAGGAAGAGACCCACATCACCAACGTGATCGACCCCTGG
GCCGGCTCCTACATGATGGAAAAGCTCACCCAGGACATGGCCGACGCCGCCTGGAAGATCATCGAAGAGGTCGAGGCGAT
GGGCGGCATGACCGCCGCCGTGGACTCGGGCTGGGCCAAGCTCAAGATCGAGGCCGCGGCTGCGGAGAAGCAGGCGCGCA
TCGATTCGGGCAAGGAGGTCATCGTCGGCGTGAACAAGTACAAGCTTGCCAAGGAAGACCCGGTGGACATCCTGGAGGTG
GACAACGTCAAGGTGCGCGAGAACCAGATCGCGCGCCTCAACGCCATCAAGGCAAAACGCGATCAAAGCGCCGTGGATCA
AGCGCTGGCTGCTCTTACTTCTGCAGCAGAGAGCGGCCAGGGCAACCTGCTGGATCTGTCGATCCAGGCGATTCGCGCAC
GCGCCACGGTGGGCGAGGTGTCCGACGCACTGGAAAAAATCTTCGGGCGCCACCGCGCCGATACGCAAAAGGTGACCGGT
GTGTACGCTGCTGCCTACGACTCGGCCGAAGGCTGGGAAAAGCTCAAGGGTGAAATCGATCAGGCCGCCGAACAGCTCGG
TCGCCGCCCGCGCGTGATGATCGCGAAGCTCGGCCAAGACGGCCACGACCGTGGCGCCAAGGTGGTGGCCACAGCCTTTG
CCGACCTGGGGTTCGATGTCGACATGGGCCCGCTGTTCCAGACCCCCGAGGAGTGCGCGCGCCAGGCCATCGAGAACGAC
GTGCACGCCGTGGGCGTCTCCACCCTGGCCGCGGGCCACAAGACGCTGGTGCCCGCCATCATCGAAGAACTGAAAAAGCA
GGGCGCCGACGACATCATCGTCTTCGTGGGCGGCGTGGTGCCCGCGCAGGACTACGACTTCCTCTACGGTGCAGGCGTCA
AGGGCGTCTATGGTCCCGGCACGCCGATCCCGGCCAGCGCCAAGGATGTGCTGGAGCAAATCAAGAAGGCGCAGGCGCAG
TGA

Upstream 100 bases:

>100_bases
TAAGAATTAATAATTACAGATCAGGTAAACTCCGCAAAATTGCGAAAACGCAGCACGCATAACCACATCTCTTTGCCATC
TTTGCGAAAGCGCCCCCGCC

Downstream 100 bases:

>100_bases
CCCCCCAGCAGCTGCAAGAAGGCATCCTGCACGGCGCGCCCGCCGTGCGGCGCCGCGCCATGGCCAAGGCCATCACGCTG
CTGGAATCCACGCGGGCCGA

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MSSHPDPIFQSADLAAWAKAAAKSAPGGDVNALNWVTPDGISVKPLYTAQDTQDLPYANTLPGFEPYLRGPQATMYAVRP
WTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFDGIPLDKVSV
SMTMNGAVLPVLAGYVVAAEEQGVSQDKLAGTIQNDILKEFMVRNTYIYPPKPSMRIIGDIIEYTSKNMPKFNSISISGY
HMQEAGANQALELAFTLADGKEYVKTAIAKGMDVDDFAGRLSFFWAIGMNFYLEIAKMRAARLLWCRIMKGFNAKNPKSL
MLRTHCQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQLIIQEETHITNVIDPW
AGSYMMEKLTQDMADAAWKIIEEVEAMGGMTAAVDSGWAKLKIEAAAAEKQARIDSGKEVIVGVNKYKLAKEDPVDILEV
DNVKVRENQIARLNAIKAKRDQSAVDQALAALTSAAESGQGNLLDLSIQAIRARATVGEVSDALEKIFGRHRADTQKVTG
VYAAAYDSAEGWEKLKGEIDQAAEQLGRRPRVMIAKLGQDGHDRGAKVVATAFADLGFDVDMGPLFQTPEECARQAIEND
VHAVGVSTLAAGHKTLVPAIIEELKKQGADDIIVFVGGVVPAQDYDFLYGAGVKGVYGPGTPIPASAKDVLEQIKKAQAQ

Sequences:

>Translated_720_residues
MSSHPDPIFQSADLAAWAKAAAKSAPGGDVNALNWVTPDGISVKPLYTAQDTQDLPYANTLPGFEPYLRGPQATMYAVRP
WTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFDGIPLDKVSV
SMTMNGAVLPVLAGYVVAAEEQGVSQDKLAGTIQNDILKEFMVRNTYIYPPKPSMRIIGDIIEYTSKNMPKFNSISISGY
HMQEAGANQALELAFTLADGKEYVKTAIAKGMDVDDFAGRLSFFWAIGMNFYLEIAKMRAARLLWCRIMKGFNAKNPKSL
MLRTHCQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQLIIQEETHITNVIDPW
AGSYMMEKLTQDMADAAWKIIEEVEAMGGMTAAVDSGWAKLKIEAAAAEKQARIDSGKEVIVGVNKYKLAKEDPVDILEV
DNVKVRENQIARLNAIKAKRDQSAVDQALAALTSAAESGQGNLLDLSIQAIRARATVGEVSDALEKIFGRHRADTQKVTG
VYAAAYDSAEGWEKLKGEIDQAAEQLGRRPRVMIAKLGQDGHDRGAKVVATAFADLGFDVDMGPLFQTPEECARQAIEND
VHAVGVSTLAAGHKTLVPAIIEELKKQGADDIIVFVGGVVPAQDYDFLYGAGVKGVYGPGTPIPASAKDVLEQIKKAQAQ
>Mature_719_residues
SSHPDPIFQSADLAAWAKAAAKSAPGGDVNALNWVTPDGISVKPLYTAQDTQDLPYANTLPGFEPYLRGPQATMYAVRPW
TIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFDGIPLDKVSVS
MTMNGAVLPVLAGYVVAAEEQGVSQDKLAGTIQNDILKEFMVRNTYIYPPKPSMRIIGDIIEYTSKNMPKFNSISISGYH
MQEAGANQALELAFTLADGKEYVKTAIAKGMDVDDFAGRLSFFWAIGMNFYLEIAKMRAARLLWCRIMKGFNAKNPKSLM
LRTHCQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQLIIQEETHITNVIDPWA
GSYMMEKLTQDMADAAWKIIEEVEAMGGMTAAVDSGWAKLKIEAAAAEKQARIDSGKEVIVGVNKYKLAKEDPVDILEVD
NVKVRENQIARLNAIKAKRDQSAVDQALAALTSAAESGQGNLLDLSIQAIRARATVGEVSDALEKIFGRHRADTQKVTGV
YAAAYDSAEGWEKLKGEIDQAAEQLGRRPRVMIAKLGQDGHDRGAKVVATAFADLGFDVDMGPLFQTPEECARQAIENDV
HAVGVSTLAAGHKTLVPAIIEELKKQGADDIIVFVGGVVPAQDYDFLYGAGVKGVYGPGTPIPASAKDVLEQIKKAQAQ

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=702, Percent_Identity=65.3846153846154, Blast_Score=966, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=711, Percent_Identity=62.1659634317862, Blast_Score=897, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=701, Percent_Identity=64.1940085592011, Blast_Score=934, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 77804; Mature: 77673

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHPDPIFQSADLAAWAKAAAKSAPGGDVNALNWVTPDGISVKPLYTAQDTQDLPYANT
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCC
LPGFEPYLRGPQATMYAVRPWTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHR
CCCCHHHHCCCCCEEEEECCEEEHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHCC
GYDSDHPRVTGDVGKAGVAIDSVEDMKILFDGIPLDKVSVSMTMNGAVLPVLAGYVVAAE
CCCCCCCEEECCCCCCCEEECCHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHEEEC
EQGVSQDKLAGTIQNDILKEFMVRNTYIYPPKPSMRIIGDIIEYTSKNMPKFNSISISGY
CCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECE
HMQEAGANQALELAFTLADGKEYVKTAIAKGMDVDDFAGRLSFFWAIGMNFYLEIAKMRA
EHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLLWCRIMKGFNAKNPKSLMLRTHCQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSL
HHHHHHHHHHCCCCCCCHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
HTNALDEAIALPTEFSARIARNTQLIIQEETHITNVIDPWAGSYMMEKLTQDMADAAWKI
HHHHHHHHHCCCCHHHHHHHCCCEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IEEVEAMGGMTAAVDSGWAKLKIEAAAAEKQARIDSGKEVIVGVNKYKLAKEDPVDILEV
HHHHHHHCCCEEECCCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCEECCCCCCCCEEEC
DNVKVRENQIARLNAIKAKRDQSAVDQALAALTSAAESGQGNLLDLSIQAIRARATVGEV
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
SDALEKIFGRHRADTQKVTGVYAAAYDSAEGWEKLKGEIDQAAEQLGRRPRVMIAKLGQD
HHHHHHHHHHCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
GHDRGAKVVATAFADLGFDVDMGPLFQTPEECARQAIENDVHAVGVSTLAAGHKTLVPAI
CCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHH
IEELKKQGADDIIVFVGGVVPAQDYDFLYGAGVKGVYGPGTPIPASAKDVLEQIKKAQAQ
HHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSHPDPIFQSADLAAWAKAAAKSAPGGDVNALNWVTPDGISVKPLYTAQDTQDLPYANT
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCC
LPGFEPYLRGPQATMYAVRPWTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHR
CCCCHHHHCCCCCEEEEECCEEEHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHCC
GYDSDHPRVTGDVGKAGVAIDSVEDMKILFDGIPLDKVSVSMTMNGAVLPVLAGYVVAAE
CCCCCCCEEECCCCCCCEEECCHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHEEEC
EQGVSQDKLAGTIQNDILKEFMVRNTYIYPPKPSMRIIGDIIEYTSKNMPKFNSISISGY
CCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECE
HMQEAGANQALELAFTLADGKEYVKTAIAKGMDVDDFAGRLSFFWAIGMNFYLEIAKMRA
EHHHCCCCCEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLLWCRIMKGFNAKNPKSLMLRTHCQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSL
HHHHHHHHHHCCCCCCCHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
HTNALDEAIALPTEFSARIARNTQLIIQEETHITNVIDPWAGSYMMEKLTQDMADAAWKI
HHHHHHHHHCCCCHHHHHHHCCCEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHH
IEEVEAMGGMTAAVDSGWAKLKIEAAAAEKQARIDSGKEVIVGVNKYKLAKEDPVDILEV
HHHHHHHCCCEEECCCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCEECCCCCCCCEEEC
DNVKVRENQIARLNAIKAKRDQSAVDQALAALTSAAESGQGNLLDLSIQAIRARATVGEV
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
SDALEKIFGRHRADTQKVTGVYAAAYDSAEGWEKLKGEIDQAAEQLGRRPRVMIAKLGQD
HHHHHHHHHHCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
GHDRGAKVVATAFADLGFDVDMGPLFQTPEECARQAIENDVHAVGVSTLAAGHKTLVPAI
CCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHH
IEELKKQGADDIIVFVGGVVPAQDYDFLYGAGVKGVYGPGTPIPASAKDVLEQIKKAQAQ
HHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8566763; 12949112 [H]