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The map label for this gene is prpE [H]

Identifier: 222111018

GI number: 222111018

Start: 1938011

End: 1939906

Strand: Direct

Name: prpE [H]

Synonym: Dtpsy_1825

Alternate gene names: 222111018

Gene position: 1938011-1939906 (Clockwise)

Preceding gene: 222111017

Following gene: 222111023

Centisome position: 51.05

GC content: 68.57

Gene sequence:

>1896_bases
ATGGCCGCCGACTATGCCGACTTCTACCGCCGCTCCATCGACGAGCGCGACGCCTTCTGGGCCGAGCAGGCTCGCCTGAT
CGACTGGCAGCAGCCGCCGCAGCAGATCTGCGACTGGAGCAACCCGCCGTTCGCCAAGTGGTTCGTGGGCGGCACCACCA
ACCTGTGCCACAACGCGGTGGACCGCCACCTGGCCGAGCGCCCGCTGCAAAACGCGCTCGTGGCCATCTCCACCGAAACC
AATACCGAGCGCGCCTACACCTACGCCGAGCTGCACCAGGAAGTGCAGCGCATGGCCGCCAGCCTGCAGACGCTGGGCGT
GCGCCAGGGCGACCGCGTGCTGATCTACATGCCCATGATTGCCGAGGCGGCGTTCGCCATGCTGGCGTGCGCGCGCATCG
GGGCCATCCATTCGGTGGTGTTCGGCGGGTTCGCGTCGTCGTCGCTGGCTTCGCGCATCGAGGACGCCGAGCCCACGGTG
ATCGTGAGCGCCGATGCGGGCTCGCGCGGCGGCAAGGTGGTGCCCTACAAGCCGCTGCTGGACGAGGCCATCGAACTGTC
GGCCCACAAGCCCGCGGCCGTGCTGTTGGTGGACCGGGGCCTCGCGCCCGCCGCCATGCGCGCGGGCCGCGACCACGACT
GGGCGGCACTGCGCGCGCGGCACATGGATGCACAGGTGCCCTGCATCTGGGTGGACGCCACGCAGCCCAGCTACACCCTG
TACACCAGCGGCACCACCGGCAAGCCCAAGGGCGTGCAGCGGGACACGGGCGGCTACACCGTGGCGCTGGCGTCCAGCAT
GCGCCACATCTTCGAGGCACAGCCGGGCGACACCTATTTCTGTACCAGCGACATCGGCTGGGTGGTGGGCCACAGCTACA
TCATCTACGGCCCGCTGATTGCCGGCATGACCACCATCCTGTACGAAGGCCTGCCGGTGCGGCCCGACGCGGGCATCTGG
TGGAGCATCGTCGAGAAATACCGGGTCACGCACATGTTCTCGGCCCCCACGGCGGTGCGCGTGCTCAAGAAGCAAGACCC
GGCCTACCTGAAGAAATACGACATCCGCAGCCTGAAGGCGCTGTGGCTGGCGGGCGAGCCCCTGGACGAGCCCACGGCGC
AGTGGATCAGCGACGCGCTGCAGGTGCCCATCATCGACAACTACTGGCAGACCGAGACCGGCTGGCCCATCCTCACGCTG
TGCAACGGCGTGCAGAAGCAAACCTCGCGCTTCGGCAGCCCGGGCAAGGCCGTGTACGGCTACAACGTCCAGCTGATCGA
CGAGGCCACGGGCGAGGAGCTGACCCAGCCCAACCAGAAGGGAGTGGTGGCCATCGAGGGGCCGCTGCCCCCTGGCTGCC
TGCAGACCGTGTGGCGCAACGATGATCGCTTCGTCAACACCTACTGGAAGAGCATTCCCGGCCGGCTGGTCTACAGCACG
TTCGACTGGGGCATCCGCGATGCCGACGGCTACTACTTCATCCTGGGCCGCACCGACGACGTGATCAACGTGGCCGGCCA
CCGCCTGGGCACGCGCGAGATCGAGGAATGCATCGCCGGCCACCCCAATGTGGCCGAGGTGGCCGTGGTGGGCGTGGCCG
ACGCGCTGAAAGGCCAGGTCGCGATGGCCTTCGCGGTGCCGCGCGATGCCTCGGGCCTCACCGACGAGGCCGCGCGCCTG
AAGCTGGAAGGCGAGGTGATGAAGCAGGTCGATACCCAACTGGGCGCCGTGGCCCGCCCGGCGCGCGTGCTGTTCGTGAC
GATGCTGCCCAAGACCCGCAGCGGCAAGCTGCTGCGCCGCGCCCTGCAGGCCGTGGCCGAGCGCCGCGACACGGGCGACC
TGACCACCATGGAAGACCCCGTGGCACTGCAGCAAATCAAGGAACTGCTGGGCTGA

Upstream 100 bases:

>100_bases
CCGCGTCAGCTTCCCGCAAGCGCGGCATGAATAATTATGAATTTTGGATAACCACCGACCCGCGCCACGTCGGCGCGGAG
GATTCCAAGGAGACAGCACC

Downstream 100 bases:

>100_bases
CGCGCAGGAATTTGCTATCGATTTAATAGCTTTCAGCGCTTGTTGGACGGGCGCTGGAAGCTTTTTTATTGCTTGATTCG
ATACGCCATCAAGCGGCTAT

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MAADYADFYRRSIDERDAFWAEQARLIDWQQPPQQICDWSNPPFAKWFVGGTTNLCHNAVDRHLAERPLQNALVAISTET
NTERAYTYAELHQEVQRMAASLQTLGVRQGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASSSLASRIEDAEPTV
IVSADAGSRGGKVVPYKPLLDEAIELSAHKPAAVLLVDRGLAPAAMRAGRDHDWAALRARHMDAQVPCIWVDATQPSYTL
YTSGTTGKPKGVQRDTGGYTVALASSMRHIFEAQPGDTYFCTSDIGWVVGHSYIIYGPLIAGMTTILYEGLPVRPDAGIW
WSIVEKYRVTHMFSAPTAVRVLKKQDPAYLKKYDIRSLKALWLAGEPLDEPTAQWISDALQVPIIDNYWQTETGWPILTL
CNGVQKQTSRFGSPGKAVYGYNVQLIDEATGEELTQPNQKGVVAIEGPLPPGCLQTVWRNDDRFVNTYWKSIPGRLVYST
FDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEECIAGHPNVAEVAVVGVADALKGQVAMAFAVPRDASGLTDEAARL
KLEGEVMKQVDTQLGAVARPARVLFVTMLPKTRSGKLLRRALQAVAERRDTGDLTTMEDPVALQQIKELLG

Sequences:

>Translated_631_residues
MAADYADFYRRSIDERDAFWAEQARLIDWQQPPQQICDWSNPPFAKWFVGGTTNLCHNAVDRHLAERPLQNALVAISTET
NTERAYTYAELHQEVQRMAASLQTLGVRQGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASSSLASRIEDAEPTV
IVSADAGSRGGKVVPYKPLLDEAIELSAHKPAAVLLVDRGLAPAAMRAGRDHDWAALRARHMDAQVPCIWVDATQPSYTL
YTSGTTGKPKGVQRDTGGYTVALASSMRHIFEAQPGDTYFCTSDIGWVVGHSYIIYGPLIAGMTTILYEGLPVRPDAGIW
WSIVEKYRVTHMFSAPTAVRVLKKQDPAYLKKYDIRSLKALWLAGEPLDEPTAQWISDALQVPIIDNYWQTETGWPILTL
CNGVQKQTSRFGSPGKAVYGYNVQLIDEATGEELTQPNQKGVVAIEGPLPPGCLQTVWRNDDRFVNTYWKSIPGRLVYST
FDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEECIAGHPNVAEVAVVGVADALKGQVAMAFAVPRDASGLTDEAARL
KLEGEVMKQVDTQLGAVARPARVLFVTMLPKTRSGKLLRRALQAVAERRDTGDLTTMEDPVALQQIKELLG
>Mature_630_residues
AADYADFYRRSIDERDAFWAEQARLIDWQQPPQQICDWSNPPFAKWFVGGTTNLCHNAVDRHLAERPLQNALVAISTETN
TERAYTYAELHQEVQRMAASLQTLGVRQGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASSSLASRIEDAEPTVI
VSADAGSRGGKVVPYKPLLDEAIELSAHKPAAVLLVDRGLAPAAMRAGRDHDWAALRARHMDAQVPCIWVDATQPSYTLY
TSGTTGKPKGVQRDTGGYTVALASSMRHIFEAQPGDTYFCTSDIGWVVGHSYIIYGPLIAGMTTILYEGLPVRPDAGIWW
SIVEKYRVTHMFSAPTAVRVLKKQDPAYLKKYDIRSLKALWLAGEPLDEPTAQWISDALQVPIIDNYWQTETGWPILTLC
NGVQKQTSRFGSPGKAVYGYNVQLIDEATGEELTQPNQKGVVAIEGPLPPGCLQTVWRNDDRFVNTYWKSIPGRLVYSTF
DWGIRDADGYYFILGRTDDVINVAGHRLGTREIEECIAGHPNVAEVAVVGVADALKGQVAMAFAVPRDASGLTDEAARLK
LEGEVMKQVDTQLGAVARPARVLFVTMLPKTRSGKLLRRALQAVAERRDTGDLTTMEDPVALQQIKELLG

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=635, Percent_Identity=42.2047244094488, Blast_Score=506, Evalue=1e-143,
Organism=Homo sapiens, GI8923896, Length=659, Percent_Identity=37.1775417298938, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI28416953, Length=631, Percent_Identity=39.1442155309033, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI253970489, Length=672, Percent_Identity=36.4583333333333, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI38505220, Length=589, Percent_Identity=27.3344651952462, Blast_Score=182, Evalue=1e-45,
Organism=Homo sapiens, GI122937307, Length=570, Percent_Identity=27.3684210526316, Blast_Score=169, Evalue=1e-41,
Organism=Homo sapiens, GI42544132, Length=526, Percent_Identity=26.2357414448669, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI115511026, Length=544, Percent_Identity=27.3897058823529, Blast_Score=167, Evalue=4e-41,
Organism=Homo sapiens, GI58082049, Length=528, Percent_Identity=26.5151515151515, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI157311624, Length=530, Percent_Identity=26.4150943396226, Blast_Score=140, Evalue=5e-33,
Organism=Homo sapiens, GI157311622, Length=530, Percent_Identity=26.4150943396226, Blast_Score=140, Evalue=5e-33,
Organism=Homo sapiens, GI31982927, Length=653, Percent_Identity=23.583460949464, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI195947378, Length=440, Percent_Identity=23.1818181818182, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI42544134, Length=358, Percent_Identity=22.6256983240223, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI187761345, Length=398, Percent_Identity=23.1155778894472, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI187761343, Length=398, Percent_Identity=23.1155778894472, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786529, Length=626, Percent_Identity=62.9392971246006, Blast_Score=821, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=633, Percent_Identity=37.5987361769352, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI221142682, Length=546, Percent_Identity=23.4432234432234, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI145693145, Length=552, Percent_Identity=22.6449275362319, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788107, Length=558, Percent_Identity=24.0143369175627, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1786810, Length=534, Percent_Identity=21.7228464419476, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=648, Percent_Identity=36.1111111111111, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71982997, Length=648, Percent_Identity=36.1111111111111, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17569507, Length=651, Percent_Identity=21.9662058371736, Blast_Score=129, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=546, Percent_Identity=25.2747252747253, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=563, Percent_Identity=23.0905861456483, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=561, Percent_Identity=21.5686274509804, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=582, Percent_Identity=21.3058419243986, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=564, Percent_Identity=24.290780141844, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=568, Percent_Identity=24.1197183098592, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=532, Percent_Identity=21.4285714285714, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319264, Length=640, Percent_Identity=37.65625, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6323182, Length=637, Percent_Identity=38.7755102040816, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6319699, Length=371, Percent_Identity=24.7978436657682, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21355225, Length=637, Percent_Identity=41.9152276295133, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24667955, Length=654, Percent_Identity=37.0030581039755, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI62472339, Length=560, Percent_Identity=38.2142857142857, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24667959, Length=527, Percent_Identity=37.3814041745731, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI21355181, Length=538, Percent_Identity=26.2081784386617, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922652, Length=558, Percent_Identity=24.3727598566308, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21358303, Length=492, Percent_Identity=22.9674796747967, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648260, Length=367, Percent_Identity=26.7029972752044, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648257, Length=379, Percent_Identity=26.3852242744063, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20130357, Length=387, Percent_Identity=25.0645994832041, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 69509; Mature: 69378

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADYADFYRRSIDERDAFWAEQARLIDWQQPPQQICDWSNPPFAKWFVGGTTNLCHNAV
CCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEECCCHHHHHHHH
DRHLAERPLQNALVAISTETNTERAYTYAELHQEVQRMAASLQTLGVRQGDRVLIYMPMI
HHHHHHCCHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHH
AEAAFAMLACARIGAIHSVVFGGFASSSLASRIEDAEPTVIVSADAGSRGGKVVPYKPLL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEECCCHHHH
DEAIELSAHKPAAVLLVDRGLAPAAMRAGRDHDWAALRARHMDAQVPCIWVDATQPSYTL
HHHHHHCCCCCEEEEEEECCCCHHHHHCCCCCCHHHHHHHCCCCCCCEEEEECCCCCEEE
YTSGTTGKPKGVQRDTGGYTVALASSMRHIFEAQPGDTYFCTSDIGWVVGHSYIIYGPLI
EECCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCEEEEECCCCHHHCCCEEEEHHHH
AGMTTILYEGLPVRPDAGIWWSIVEKYRVTHMFSAPTAVRVLKKQDPAYLKKYDIRSLKA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEECCCHHHHHHHCCCCHHHHHHHHHHHHE
LWLAGEPLDEPTAQWISDALQVPIIDNYWQTETGWPILTLCNGVQKQTSRFGSPGKAVYG
EEECCCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
YNVQLIDEATGEELTQPNQKGVVAIEGPLPPGCLQTVWRNDDRFVNTYWKSIPGRLVYST
EEEEEEECCCCCHHCCCCCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHCCCCCEEEEE
FDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEECIAGHPNVAEVAVVGVADALKGQV
ECCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHCCCCCHHHEEEEEHHHHHCCCE
AMAFAVPRDASGLTDEAARLKLEGEVMKQVDTQLGAVARPARVLFVTMLPKTRSGKLLRR
EEEEECCCCCCCCCCHHHHEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
ALQAVAERRDTGDLTTMEDPVALQQIKELLG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
AADYADFYRRSIDERDAFWAEQARLIDWQQPPQQICDWSNPPFAKWFVGGTTNLCHNAV
CCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEECCCHHHHHHHH
DRHLAERPLQNALVAISTETNTERAYTYAELHQEVQRMAASLQTLGVRQGDRVLIYMPMI
HHHHHHCCHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHH
AEAAFAMLACARIGAIHSVVFGGFASSSLASRIEDAEPTVIVSADAGSRGGKVVPYKPLL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEECCCHHHH
DEAIELSAHKPAAVLLVDRGLAPAAMRAGRDHDWAALRARHMDAQVPCIWVDATQPSYTL
HHHHHHCCCCCEEEEEEECCCCHHHHHCCCCCCHHHHHHHCCCCCCCEEEEECCCCCEEE
YTSGTTGKPKGVQRDTGGYTVALASSMRHIFEAQPGDTYFCTSDIGWVVGHSYIIYGPLI
EECCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCEEEEECCCCHHHCCCEEEEHHHH
AGMTTILYEGLPVRPDAGIWWSIVEKYRVTHMFSAPTAVRVLKKQDPAYLKKYDIRSLKA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEECCCHHHHHHHCCCCHHHHHHHHHHHHE
LWLAGEPLDEPTAQWISDALQVPIIDNYWQTETGWPILTLCNGVQKQTSRFGSPGKAVYG
EEECCCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
YNVQLIDEATGEELTQPNQKGVVAIEGPLPPGCLQTVWRNDDRFVNTYWKSIPGRLVYST
EEEEEEECCCCCHHCCCCCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHCCCCCEEEEE
FDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEECIAGHPNVAEVAVVGVADALKGQV
ECCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHCCCCCHHHEEEEEHHHHHCCCE
AMAFAVPRDASGLTDEAARLKLEGEVMKQVDTQLGAVARPARVLFVTMLPKTRSGKLLRR
EEEEECCCCCCCCCCHHHHEEECHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
ALQAVAERRDTGDLTTMEDPVALQQIKELLG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9006051; 10411265; 11677609; 10482501 [H]