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The map label for this gene is braC [H]

Identifier: 222110921

GI number: 222110921

Start: 1834169

End: 1835296

Strand: Direct

Name: braC [H]

Synonym: Dtpsy_1728

Alternate gene names: 222110921

Gene position: 1834169-1835296 (Clockwise)

Preceding gene: 222110920

Following gene: 222110923

Centisome position: 48.31

GC content: 60.28

Gene sequence:

>1128_bases
ATGCAACTGAAGTTGAAAATGACCGTGGCTGCTGCGATCGCAGCGGTTGCCGGTGTGGCCGCTGCTCAAGAGGTGGTGAA
GATTGGTCACGTGGCACCGGTCTCCGGCGCCCAGGCGCACTACGGCAAGGACAACGAAAACGGCGCTCGCATGGCCATTG
AAGAGCTGAACGCCCAGGGCGTCACGATCGGTGGCAAGAAGGTGAAGCTGGAATTGGTGGCGGAGGACGATGCCGCTGAT
CCCAAGCAAGGCACGGCGGCCGCACAGAAGTTGTGCGATGCCAAGGTCGCGGGGGTGGTCGGGCACCTGAACTCGGGCAC
CACGATCCCTGCTTCCAAGGTCTACAACGACTGCGGGATCCCTCACGTCACTGGCGCCGCAACCAACCCCAACCTGACGC
GCCCCGGCTACAAAACCACCTTCCGCATCATTGCGAACGACAACGCTCTGGGTGCTGGACTGGCGGCTTACGCGGCGGAA
ACCCTGAAGCTGAAGACTGTGGCCATCGTGGATGACCGCACCGCTTATGGCCAGGGCGTTGCCAACGTGTTCAAGCGAGT
GGCAGCCGAGAAAGGCATGAAGGTTGTGGACGAACAGTTCACCACGGACAAGGCGACGGACTTCATGGCCATTCTGACGG
CCATCAAGGCCAAGAACCCCGATGCGATCTTCTTCGGCGGCATGGATCCTCAGGCGGGTCCCATGTTGCGCCAGATGGAG
CAACTGGGTTTGGCCAACACGAAGTATTTCGGTGGGGATGGCGTATGCACCACGGAAATTGCCAAGCTGGCGGCAGGCGC
CAAGACGCTGGCTAACGTGATTTGCGCAGAGGGTGGCGCATCCCTGGCCAAGATGCCTGGCGGCACGGCCTGGAAGGCCA
AATACGACGCCAAGTACCCCAACCAGTTCCAGGTCTACAGCCCGTACACCTATGACGCGACCATGCTGCTGGTGGACGCT
ATGAAGCGCGCCGGCTCCTGGGACCCGAAGGTGTATATCCCCGAGTTGCTCAAGTCCAACTACAAGGGCGTGACCGCGAA
CATTGCGTTTGAACCCAATGGCGAAATGAAGAACCCGGCCATTACGCTGTACACCTACAAGGAAGGCAAGAAGACGCCGT
TGAACTGA

Upstream 100 bases:

>100_bases
CTCCTTGTGTTCCCTAGTGATGTGGAACATAAATTGCGATATGTTTCTCCGCGTTGGTTCACAACAATATTTCCAGCGCT
TTTCTTAATTGGAGATCCAT

Downstream 100 bases:

>100_bases
TCGACCTGTTCCGCCTCGCCTTGAGTGCGGATCTCGTAAAAAAGGGCTCCTTGGGGAGCCCTTTCTCTTGATATCAGCAA
CCGTTCAGCGTCGGACCTGC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MQLKLKMTVAAAIAAVAGVAAAQEVVKIGHVAPVSGAQAHYGKDNENGARMAIEELNAQGVTIGGKKVKLELVAEDDAAD
PKQGTAAAQKLCDAKVAGVVGHLNSGTTIPASKVYNDCGIPHVTGAATNPNLTRPGYKTTFRIIANDNALGAGLAAYAAE
TLKLKTVAIVDDRTAYGQGVANVFKRVAAEKGMKVVDEQFTTDKATDFMAILTAIKAKNPDAIFFGGMDPQAGPMLRQME
QLGLANTKYFGGDGVCTTEIAKLAAGAKTLANVICAEGGASLAKMPGGTAWKAKYDAKYPNQFQVYSPYTYDATMLLVDA
MKRAGSWDPKVYIPELLKSNYKGVTANIAFEPNGEMKNPAITLYTYKEGKKTPLN

Sequences:

>Translated_375_residues
MQLKLKMTVAAAIAAVAGVAAAQEVVKIGHVAPVSGAQAHYGKDNENGARMAIEELNAQGVTIGGKKVKLELVAEDDAAD
PKQGTAAAQKLCDAKVAGVVGHLNSGTTIPASKVYNDCGIPHVTGAATNPNLTRPGYKTTFRIIANDNALGAGLAAYAAE
TLKLKTVAIVDDRTAYGQGVANVFKRVAAEKGMKVVDEQFTTDKATDFMAILTAIKAKNPDAIFFGGMDPQAGPMLRQME
QLGLANTKYFGGDGVCTTEIAKLAAGAKTLANVICAEGGASLAKMPGGTAWKAKYDAKYPNQFQVYSPYTYDATMLLVDA
MKRAGSWDPKVYIPELLKSNYKGVTANIAFEPNGEMKNPAITLYTYKEGKKTPLN
>Mature_375_residues
MQLKLKMTVAAAIAAVAGVAAAQEVVKIGHVAPVSGAQAHYGKDNENGARMAIEELNAQGVTIGGKKVKLELVAEDDAAD
PKQGTAAAQKLCDAKVAGVVGHLNSGTTIPASKVYNDCGIPHVTGAATNPNLTRPGYKTTFRIIANDNALGAGLAAYAAE
TLKLKTVAIVDDRTAYGQGVANVFKRVAAEKGMKVVDEQFTTDKATDFMAILTAIKAKNPDAIFFGGMDPQAGPMLRQME
QLGLANTKYFGGDGVCTTEIAKLAAGAKTLANVICAEGGASLAKMPGGTAWKAKYDAKYPNQFQVYSPYTYDATMLLVDA
MKRAGSWDPKVYIPELLKSNYKGVTANIAFEPNGEMKNPAITLYTYKEGKKTPLN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=379, Percent_Identity=32.1899736147757, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI48994941, Length=264, Percent_Identity=35.9848484848485, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39445; Mature: 39445

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLKLKMTVAAAIAAVAGVAAAQEVVKIGHVAPVSGAQAHYGKDNENGARMAIEELNAQG
CEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
VTIGGKKVKLELVAEDDAADPKQGTAAAQKLCDAKVAGVVGHLNSGTTIPASKVYNDCGI
EEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCC
PHVTGAATNPNLTRPGYKTTFRIIANDNALGAGLAAYAAETLKLKTVAIVDDRTAYGQGV
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEEEEEEECCCHHHHHH
ANVFKRVAAEKGMKVVDEQFTTDKATDFMAILTAIKAKNPDAIFFGGMDPQAGPMLRQME
HHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHH
QLGLANTKYFGGDGVCTTEIAKLAAGAKTLANVICAEGGASLAKMPGGTAWKAKYDAKYP
HHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCEEEEEECCCCC
NQFQVYSPYTYDATMLLVDAMKRAGSWDPKVYIPELLKSNYKGVTANIAFEPNGEMKNPA
CCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCEEEEEEECCCCCCCCCE
ITLYTYKEGKKTPLN
EEEEEECCCCCCCCC
>Mature Secondary Structure
MQLKLKMTVAAAIAAVAGVAAAQEVVKIGHVAPVSGAQAHYGKDNENGARMAIEELNAQG
CEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
VTIGGKKVKLELVAEDDAADPKQGTAAAQKLCDAKVAGVVGHLNSGTTIPASKVYNDCGI
EEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCC
PHVTGAATNPNLTRPGYKTTFRIIANDNALGAGLAAYAAETLKLKTVAIVDDRTAYGQGV
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEEEEEEECCCHHHHHH
ANVFKRVAAEKGMKVVDEQFTTDKATDFMAILTAIKAKNPDAIFFGGMDPQAGPMLRQME
HHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHH
QLGLANTKYFGGDGVCTTEIAKLAAGAKTLANVICAEGGASLAKMPGGTAWKAKYDAKYP
HHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCEEEEEECCCCC
NQFQVYSPYTYDATMLLVDAMKRAGSWDPKVYIPELLKSNYKGVTANIAFEPNGEMKNPA
CCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCEEEEEEECCCCCCCCCE
ITLYTYKEGKKTPLN
EEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]