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The map label for this gene is ligA

Identifier: 222110866

GI number: 222110866

Start: 1771458

End: 1773680

Strand: Direct

Name: ligA

Synonym: Dtpsy_1672

Alternate gene names: 222110866

Gene position: 1771458-1773680 (Clockwise)

Preceding gene: 222110865

Following gene: 222110867

Centisome position: 46.66

GC content: 70.49

Gene sequence:

>2223_bases
ATGGGACCGGGCCTGACCCTTTCCGGCATGACTGAACAATCCAGCCTTTTTCCGGCTCCCGCGCCCAAGGGGTCTGCGCT
GGTAGCTGCAAAAATCAAAGCGCTGCGCGAGCAGTTGAACCGCTGGGCGCACGAGTACTACGTCCAGGACACGCCGAGCG
TGCCCGATGCCGAGTATGACCGCGCGTTCCAGGAATTGCAGGCGCTGGAGGGCGCTTACCCCGACCTGGTGACGCCCGAC
TCGCCCACGCAGCGCGTGCTGGGCGCGGTGCTGGACGGGCTGACACCGGTGCGCCACCGCGTGCCCATGCTCAGCATCCG
CACCGAAACCGACACCGAGGCCAGCGGCGCCCAAGCCTTCGACGCGCGCGTGCGCCGTGAACTGAAGCTGCCGCCCGATG
CGCCGCCGGTGGAATACGTGGCCGAACCCAAGTTCGACGGCCTGGCGATGAGCCTGCGCTACGAGGGCGGGCGCCTGGTG
CAGGCCGCCACGCGCGGTGACGGCGAGGTGGGCGAGGACGTGACGCACAACGTGCGCACCATCCGCCAGATCCCCCTGAG
CCTGCCCGCCGGCGTGCCGCGGGTGCTGGAGGTGCGCGGGGAGGTCTACATGCGCCGCGCGGACTTCGACGCACTCAACG
AGCGCCAGCGCGAGAAGGGCGACAAGACCTTCGTCAACCCGCGCAACGCCGCCGCCGGCGCGGTGCGCCAGTTGGACTCA
GGCATCACCGCGCAGCGGCCGCTGTCCTTCTTCGCCTACGGCCTGGGCGAGGTCACGCCGGCCGCCGAGGGCGGGCCCGA
CTTCGGCACGCACTTCGGCATGCTGCAGCAGCTCAAGGAATGGGGCTTTCCGGTTGCAGCCCAGGTGCAGATTGCGCAAG
GCGCTTCTGAACTCATAGCGTTCCATCAGCGTGTGGGGGCCGAACGGGACGCGCTGCCCTACGACATCGACGGCGTGGTC
TACAAGGTCAACAGCCTGGCACTGCAGCGCCAGCTGGGCTTTGTGACGCGCGAGCCGCGCTGGGCCGTGGCTCACAAATA
CCCGGCCCAGGAGATGGTCACGCGCGTGGAAGGTATCGACGTGCAGGTGGGCCGCACGGGCAAGCTGACCCCTGTGGCGC
GACTCGCGCCGGTGTTCGTGGGCGGCGTCACGGTGACCAATGCCACGCTGCACAACCTGTTCGAGCTGCGCCGCAAGAAA
GTGCGCGTGGGCGACCAGGTGATCGTGCGCCGCGCGGGCGACGTGATTCCCGAGGTGGTGGGCGTGGTGCCGGCGGGCGC
AGGCCTGGCAGTGCTGGCGGGCTCCGACGCGCTGGTGGACGCCGCCAGCAGCGCGGACGGCACCGCACCCGCGCAGCCGC
TGGCGGGGCCGCGCCAGCCCTACGTGCCCAACTTCCGCATGCCGCGCCAGTGCCCCATCTGCGGCAGCGCCGTGGTGCGC
GAGCCGGGCGAGGTCAACCACCGCTGCTCCGGCGGGCTGTTCTGCCCCGCGCAGCGCAAACAGGCGGTGCTGCACTTCGC
CCAGCGCCGCGCCATGGACATCGAGGGCCTGGGCGAGAAGCTGGTGGACCAGCTCGTCGAGGGCCACGTGATCCGCACGC
TGCCCGACCTGTACCGGCTCGGGCTGTCCAGCCTTGCGCAGCTCGACCGCATGGCCGAGAAGTCCGCGCAGAACGTGCTG
GATGCGCTCGACAAATCCAAGCGCACCACGCTGGCGCGCTTTCTCTTCGGCCTGGGCATCCGCCATGTGGGCGAGGCCAC
GGCCAAGGACCTGGCACGCCACTTCGGCCGGCTCGACGCCATCATGGATGCGGGCGTGGAACAGTTGCTGCAGGTGAACG
ACGTGGGGCCCGTGGTGGCCGAGGCCATCCACACCTTCTTCGCGCAACCGCACAACCGCGAGGTGGTGGAGCAGCTGCGC
GCCTGCGGCGTCACCTGGGACGAGAGCGAGCCCGCTGCCGCCGCCACGCTGCCCCTGGCGGGCATGACCGTGGTGCTGAC
CGGCACGCTGCCCACCTTGGGCCGCGACGCAGCCAAGGACCTGCTGGAATCCGCCGGTGCCAAGGTGGCGGGCTCGGTCA
GCAAGAAGACGCACTACGTGGTGGCCGGCGCCGACGCGGGCAGCAAGCTCGCCAAGGCGCAGGAACTGGGCATCCCCGTG
CTGGACGAGGACGGGTTGCGTGCGTTGCTGCGGGGCGCGCCGCCCAACGCAGGTGGGGGCTAG

Upstream 100 bases:

>100_bases
GCTTTCCTGGGGCCCGCGTGTCCGCGCCGAAGCAGGAACCGTACGGCGCGTGCGTGACCTACGTCTGGGACCCGTCGGGC
GTGCTGCTGCACCTCGCCCA

Downstream 100 bases:

>100_bases
ATGGCACTGACCCGCCGCGCCCCACGGCTGGGCCTTGCGTTGGGCAGCGGCTCGGCGCGCGGCTGGGCGCACATCGGCGT
GCTGCAGGCGCTGCGGGAGG

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 740; Mature: 739

Protein sequence:

>740_residues
MGPGLTLSGMTEQSSLFPAPAPKGSALVAAKIKALREQLNRWAHEYYVQDTPSVPDAEYDRAFQELQALEGAYPDLVTPD
SPTQRVLGAVLDGLTPVRHRVPMLSIRTETDTEASGAQAFDARVRRELKLPPDAPPVEYVAEPKFDGLAMSLRYEGGRLV
QAATRGDGEVGEDVTHNVRTIRQIPLSLPAGVPRVLEVRGEVYMRRADFDALNERQREKGDKTFVNPRNAAAGAVRQLDS
GITAQRPLSFFAYGLGEVTPAAEGGPDFGTHFGMLQQLKEWGFPVAAQVQIAQGASELIAFHQRVGAERDALPYDIDGVV
YKVNSLALQRQLGFVTREPRWAVAHKYPAQEMVTRVEGIDVQVGRTGKLTPVARLAPVFVGGVTVTNATLHNLFELRRKK
VRVGDQVIVRRAGDVIPEVVGVVPAGAGLAVLAGSDALVDAASSADGTAPAQPLAGPRQPYVPNFRMPRQCPICGSAVVR
EPGEVNHRCSGGLFCPAQRKQAVLHFAQRRAMDIEGLGEKLVDQLVEGHVIRTLPDLYRLGLSSLAQLDRMAEKSAQNVL
DALDKSKRTTLARFLFGLGIRHVGEATAKDLARHFGRLDAIMDAGVEQLLQVNDVGPVVAEAIHTFFAQPHNREVVEQLR
ACGVTWDESEPAAAATLPLAGMTVVLTGTLPTLGRDAAKDLLESAGAKVAGSVSKKTHYVVAGADAGSKLAKAQELGIPV
LDEDGLRALLRGAPPNAGGG

Sequences:

>Translated_740_residues
MGPGLTLSGMTEQSSLFPAPAPKGSALVAAKIKALREQLNRWAHEYYVQDTPSVPDAEYDRAFQELQALEGAYPDLVTPD
SPTQRVLGAVLDGLTPVRHRVPMLSIRTETDTEASGAQAFDARVRRELKLPPDAPPVEYVAEPKFDGLAMSLRYEGGRLV
QAATRGDGEVGEDVTHNVRTIRQIPLSLPAGVPRVLEVRGEVYMRRADFDALNERQREKGDKTFVNPRNAAAGAVRQLDS
GITAQRPLSFFAYGLGEVTPAAEGGPDFGTHFGMLQQLKEWGFPVAAQVQIAQGASELIAFHQRVGAERDALPYDIDGVV
YKVNSLALQRQLGFVTREPRWAVAHKYPAQEMVTRVEGIDVQVGRTGKLTPVARLAPVFVGGVTVTNATLHNLFELRRKK
VRVGDQVIVRRAGDVIPEVVGVVPAGAGLAVLAGSDALVDAASSADGTAPAQPLAGPRQPYVPNFRMPRQCPICGSAVVR
EPGEVNHRCSGGLFCPAQRKQAVLHFAQRRAMDIEGLGEKLVDQLVEGHVIRTLPDLYRLGLSSLAQLDRMAEKSAQNVL
DALDKSKRTTLARFLFGLGIRHVGEATAKDLARHFGRLDAIMDAGVEQLLQVNDVGPVVAEAIHTFFAQPHNREVVEQLR
ACGVTWDESEPAAAATLPLAGMTVVLTGTLPTLGRDAAKDLLESAGAKVAGSVSKKTHYVVAGADAGSKLAKAQELGIPV
LDEDGLRALLRGAPPNAGGG
>Mature_739_residues
GPGLTLSGMTEQSSLFPAPAPKGSALVAAKIKALREQLNRWAHEYYVQDTPSVPDAEYDRAFQELQALEGAYPDLVTPDS
PTQRVLGAVLDGLTPVRHRVPMLSIRTETDTEASGAQAFDARVRRELKLPPDAPPVEYVAEPKFDGLAMSLRYEGGRLVQ
AATRGDGEVGEDVTHNVRTIRQIPLSLPAGVPRVLEVRGEVYMRRADFDALNERQREKGDKTFVNPRNAAAGAVRQLDSG
ITAQRPLSFFAYGLGEVTPAAEGGPDFGTHFGMLQQLKEWGFPVAAQVQIAQGASELIAFHQRVGAERDALPYDIDGVVY
KVNSLALQRQLGFVTREPRWAVAHKYPAQEMVTRVEGIDVQVGRTGKLTPVARLAPVFVGGVTVTNATLHNLFELRRKKV
RVGDQVIVRRAGDVIPEVVGVVPAGAGLAVLAGSDALVDAASSADGTAPAQPLAGPRQPYVPNFRMPRQCPICGSAVVRE
PGEVNHRCSGGLFCPAQRKQAVLHFAQRRAMDIEGLGEKLVDQLVEGHVIRTLPDLYRLGLSSLAQLDRMAEKSAQNVLD
ALDKSKRTTLARFLFGLGIRHVGEATAKDLARHFGRLDAIMDAGVEQLLQVNDVGPVVAEAIHTFFAQPHNREVVEQLRA
CGVTWDESEPAAAATLPLAGMTVVLTGTLPTLGRDAAKDLLESAGAKVAGSVSKKTHYVVAGADAGSKLAKAQELGIPVL
DEDGLRALLRGAPPNAGGG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=77, Percent_Identity=51.9480519480519, Blast_Score=77, Evalue=6e-14,
Organism=Escherichia coli, GI1788750, Length=706, Percent_Identity=51.1331444759207, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=420, Percent_Identity=26.1904761904762, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324791, Length=83, Percent_Identity=45.7831325301205, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ACIET (B9MIW4)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002553130.1
- ProteinModelPortal:   B9MIW4
- GeneID:   7384746
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_1672
- ProtClustDB:   CLSK951442
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 79295; Mature: 79164

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 144-144 BINDING 142-142 BINDING 165-165 BINDING 201-201 BINDING 322-322 BINDING 346-346

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPGLTLSGMTEQSSLFPAPAPKGSALVAAKIKALREQLNRWAHEYYVQDTPSVPDAEYD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RAFQELQALEGAYPDLVTPDSPTQRVLGAVLDGLTPVRHRVPMLSIRTETDTEASGAQAF
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCCHHHH
DARVRRELKLPPDAPPVEYVAEPKFDGLAMSLRYEGGRLVQAATRGDGEVGEDVTHNVRT
HHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHH
IRQIPLSLPAGVPRVLEVRGEVYMRRADFDALNERQREKGDKTFVNPRNAAAGAVRQLDS
HHHCCCCCCCCCHHHHHHHHHHHEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
GITAQRPLSFFAYGLGEVTPAAEGGPDFGTHFGMLQQLKEWGFPVAAQVQIAQGASELIA
CCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHH
FHQRVGAERDALPYDIDGVVYKVNSLALQRQLGFVTREPRWAVAHKYPAQEMVTRVEGID
HHHHHCCCCCCCCCCCCHHEEHHHHHHHHHHHCCCCCCCCEEHHCCCCHHHHHHHHCCCE
VQVGRTGKLTPVARLAPVFVGGVTVTNATLHNLFELRRKKVRVGDQVIVRRAGDVIPEVV
EEECCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GVVPAGAGLAVLAGSDALVDAASSADGTAPAQPLAGPRQPYVPNFRMPRQCPICGSAVVR
HHCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHC
EPGEVNHRCSGGLFCPAQRKQAVLHFAQRRAMDIEGLGEKLVDQLVEGHVIRTLPDLYRL
CCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
GLSSLAQLDRMAEKSAQNVLDALDKSKRTTLARFLFGLGIRHVGEATAKDLARHFGRLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMDAGVEQLLQVNDVGPVVAEAIHTFFAQPHNREVVEQLRACGVTWDESEPAAAATLPLA
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCC
GMTVVLTGTLPTLGRDAAKDLLESAGAKVAGSVSKKTHYVVAGADAGSKLAKAQELGIPV
CEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCC
LDEDGLRALLRGAPPNAGGG
CCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GPGLTLSGMTEQSSLFPAPAPKGSALVAAKIKALREQLNRWAHEYYVQDTPSVPDAEYD
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RAFQELQALEGAYPDLVTPDSPTQRVLGAVLDGLTPVRHRVPMLSIRTETDTEASGAQAF
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCCHHHH
DARVRRELKLPPDAPPVEYVAEPKFDGLAMSLRYEGGRLVQAATRGDGEVGEDVTHNVRT
HHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHH
IRQIPLSLPAGVPRVLEVRGEVYMRRADFDALNERQREKGDKTFVNPRNAAAGAVRQLDS
HHHCCCCCCCCCHHHHHHHHHHHEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
GITAQRPLSFFAYGLGEVTPAAEGGPDFGTHFGMLQQLKEWGFPVAAQVQIAQGASELIA
CCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHH
FHQRVGAERDALPYDIDGVVYKVNSLALQRQLGFVTREPRWAVAHKYPAQEMVTRVEGID
HHHHHCCCCCCCCCCCCHHEEHHHHHHHHHHHCCCCCCCCEEHHCCCCHHHHHHHHCCCE
VQVGRTGKLTPVARLAPVFVGGVTVTNATLHNLFELRRKKVRVGDQVIVRRAGDVIPEVV
EEECCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GVVPAGAGLAVLAGSDALVDAASSADGTAPAQPLAGPRQPYVPNFRMPRQCPICGSAVVR
HHCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHC
EPGEVNHRCSGGLFCPAQRKQAVLHFAQRRAMDIEGLGEKLVDQLVEGHVIRTLPDLYRL
CCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
GLSSLAQLDRMAEKSAQNVLDALDKSKRTTLARFLFGLGIRHVGEATAKDLARHFGRLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMDAGVEQLLQVNDVGPVVAEAIHTFFAQPHNREVVEQLRACGVTWDESEPAAAATLPLA
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCC
GMTVVLTGTLPTLGRDAAKDLLESAGAKVAGSVSKKTHYVVAGADAGSKLAKAQELGIPV
CEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCC
LDEDGLRALLRGAPPNAGGG
CCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA