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The map label for this gene is gatA [H]

Identifier: 222110821

GI number: 222110821

Start: 1715620

End: 1717170

Strand: Direct

Name: gatA [H]

Synonym: Dtpsy_1626

Alternate gene names: 222110821

Gene position: 1715620-1717170 (Clockwise)

Preceding gene: 222110820

Following gene: 222110822

Centisome position: 45.19

GC content: 72.15

Gene sequence:

>1551_bases
ATGACTGCTGATCCCACGCTGGTGGAACTGTCCGCCAATGAACTGCGCCACCGCATCGGCACGGGCGAGCTCTCGCCCGT
CGAGCTGCTGCAGGCCTGCATCGACCGCATCGAGGCGGTGAACCCCTATGTGAACGCCGTCACCGCCACCTGCTACGACC
GCGCCCGCGCCGAGGCGCAGGCCGCCGAGCGGGCGGTGCGCGCCGGCGAGCCCCTCGGCCTGCTGCACGGGCTGCCCATG
GGCGTGAAGGATCTGGAGGCCACCGCCGGCCTGCTGACCACCTACGGCTCGCCCATCTACCGCGAGAACATCCCTGCGCA
GGACAACGTGCTGGTGGCGCGCCTGCGCGCGGCCGGCGCCATCGTCACGGGCAAGACCAATATTCCCGAGATGGGCGCGG
GCGCCAATTCGCGCAACGTGGTCTGGGGCGCCACGGGTAACCCGTTCGATCCGCGCCTGAACGCGGGCGGCTCGTCCGGC
GGGTCGGCCGCGGCGCTGGCCTGCGACATGTTGCCGGTGTGCACGGGCTCGGACACGGGCGGCTCGCTGCGCATCCCTGC
GGCCAAGTGCGGCGTGGTGGGTTTTCGGCCGTCGCCCGGCGTGGTGCCCAGCTCGCGCAAGCTGTTGGGCTGGACGCCGA
TCTCCGTGGTCGGCCCCATGGGCCGCACGATGGCCGAGGCCTGCCTGCAGCTGGCCGCATCGGCCGGCCTGTCGGCGGGC
GACCCGCTGACCTACCCCCTGGACCCCCACAGCTTCCTGCACCTGGAGCCGGCCGACCTGGGCGGCCTGCGCGTGGGCTA
CACCGAAGACTTCGGCGCCTGCGCGGTGGACGACGGCATCCGCGCGGTGTTCCGCGCCAAGATCGGCGCCATGCGCCACC
TGTTCCGCTCTTGCGAGGAGATCCGGCTGGACCTGGGCGACGTGCACCGCTGCTTCGACGTGCTGCGGGCCGAGAGCTTC
TTGGCCGGCATGCGCGAGGCCTACGAGCGCGACCCCGCCAGCCTGGGGCCCAACACGCGCGCCAACTACGAGATGGGCGC
GGCCATGTCGCTGCTGGACAGCGCCTGGGCGCAGGCCGAGCAGACGCGCATCCTGGCGCGCTTCCAGGAGGCGTTTGCCA
ACTTCGACGTGATCCTGGCACCCACCACGCCGGTCTCGCCCTTCCCGTGGACCGCGCTGTACGCCAGCCATATCAACGGT
GAACCCCAGGCCAACTACTACCGCTGGCTGGCACTGACCTATGTGACCACGCTGACCACGCACCCCGCGCTCAGCGTGCC
CTGCGGGCGCGACACGGCCGGCATGCCGTTCGGCCTGCAGGTGGTGGGGCGCTTTCGCGACGACCATCGCCTACTGTCCA
TCGGCCTGGGGCTGGAGCAAGCCTTTGCCCGCATCGATGGCCTGCAGCGCCCGCGGCCCGACCTGGCCCGGCTGGGGCCG
GTGCAACCCGCGCTCACCTCCATCGTCACCCACGCGCCGGACCCCGATGACGCGCGCGGCGTGCCGCGGCTGGGCGCACG
CGGTACCGCGGAGCAGGCGTCGGCGGTCTGA

Upstream 100 bases:

>100_bases
CGGGAGCTGCCGCTGATGCGTGCCATTGCCCAGCGCGCGGGCATCACCGCCGACTGAGGCGCGGCTCATCCACAATGGCC
CCTGGACCTTCTTCACGACC

Downstream 100 bases:

>100_bases
CAGTGATTGGCGAGGGAACTCATGCAACCCTATGACTTTGTGATCATCGGCGCGGGCATTGCCGGCGCCTCCGCCGGTTG
GGAACTGGCCGCGCACCGCC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTADPTLVELSANELRHRIGTGELSPVELLQACIDRIEAVNPYVNAVTATCYDRARAEAQAAERAVRAGEPLGLLHGLPM
GVKDLEATAGLLTTYGSPIYRENIPAQDNVLVARLRAAGAIVTGKTNIPEMGAGANSRNVVWGATGNPFDPRLNAGGSSG
GSAAALACDMLPVCTGSDTGGSLRIPAAKCGVVGFRPSPGVVPSSRKLLGWTPISVVGPMGRTMAEACLQLAASAGLSAG
DPLTYPLDPHSFLHLEPADLGGLRVGYTEDFGACAVDDGIRAVFRAKIGAMRHLFRSCEEIRLDLGDVHRCFDVLRAESF
LAGMREAYERDPASLGPNTRANYEMGAAMSLLDSAWAQAEQTRILARFQEAFANFDVILAPTTPVSPFPWTALYASHING
EPQANYYRWLALTYVTTLTTHPALSVPCGRDTAGMPFGLQVVGRFRDDHRLLSIGLGLEQAFARIDGLQRPRPDLARLGP
VQPALTSIVTHAPDPDDARGVPRLGARGTAEQASAV

Sequences:

>Translated_516_residues
MTADPTLVELSANELRHRIGTGELSPVELLQACIDRIEAVNPYVNAVTATCYDRARAEAQAAERAVRAGEPLGLLHGLPM
GVKDLEATAGLLTTYGSPIYRENIPAQDNVLVARLRAAGAIVTGKTNIPEMGAGANSRNVVWGATGNPFDPRLNAGGSSG
GSAAALACDMLPVCTGSDTGGSLRIPAAKCGVVGFRPSPGVVPSSRKLLGWTPISVVGPMGRTMAEACLQLAASAGLSAG
DPLTYPLDPHSFLHLEPADLGGLRVGYTEDFGACAVDDGIRAVFRAKIGAMRHLFRSCEEIRLDLGDVHRCFDVLRAESF
LAGMREAYERDPASLGPNTRANYEMGAAMSLLDSAWAQAEQTRILARFQEAFANFDVILAPTTPVSPFPWTALYASHING
EPQANYYRWLALTYVTTLTTHPALSVPCGRDTAGMPFGLQVVGRFRDDHRLLSIGLGLEQAFARIDGLQRPRPDLARLGP
VQPALTSIVTHAPDPDDARGVPRLGARGTAEQASAV
>Mature_515_residues
TADPTLVELSANELRHRIGTGELSPVELLQACIDRIEAVNPYVNAVTATCYDRARAEAQAAERAVRAGEPLGLLHGLPMG
VKDLEATAGLLTTYGSPIYRENIPAQDNVLVARLRAAGAIVTGKTNIPEMGAGANSRNVVWGATGNPFDPRLNAGGSSGG
SAAALACDMLPVCTGSDTGGSLRIPAAKCGVVGFRPSPGVVPSSRKLLGWTPISVVGPMGRTMAEACLQLAASAGLSAGD
PLTYPLDPHSFLHLEPADLGGLRVGYTEDFGACAVDDGIRAVFRAKIGAMRHLFRSCEEIRLDLGDVHRCFDVLRAESFL
AGMREAYERDPASLGPNTRANYEMGAAMSLLDSAWAQAEQTRILARFQEAFANFDVILAPTTPVSPFPWTALYASHINGE
PQANYYRWLALTYVTTLTTHPALSVPCGRDTAGMPFGLQVVGRFRDDHRLLSIGLGLEQAFARIDGLQRPRPDLARLGPV
QPALTSIVTHAPDPDDARGVPRLGARGTAEQASAV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=502, Percent_Identity=26.4940239043825, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI195972892, Length=245, Percent_Identity=33.469387755102, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI166795287, Length=183, Percent_Identity=31.1475409836066, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=510, Percent_Identity=27.2549019607843, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17543272, Length=428, Percent_Identity=26.6355140186916, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=302, Percent_Identity=28.1456953642384, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=192, Percent_Identity=31.25, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=315, Percent_Identity=27.3015873015873, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=192, Percent_Identity=31.25, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=31.7708333333333, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=31.7708333333333, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=420, Percent_Identity=24.2857142857143, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319685, Length=140, Percent_Identity=37.8571428571429, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320448, Length=218, Percent_Identity=28.4403669724771, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=27.4028629856851, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24652985, Length=507, Percent_Identity=27.810650887574, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922090, Length=507, Percent_Identity=27.810650887574, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24652981, Length=507, Percent_Identity=27.810650887574, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24652983, Length=507, Percent_Identity=27.810650887574, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648435, Length=503, Percent_Identity=26.441351888668, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24648437, Length=503, Percent_Identity=26.441351888668, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI45550774, Length=503, Percent_Identity=26.441351888668, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24644968, Length=503, Percent_Identity=26.2425447316103, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078093, Length=499, Percent_Identity=27.6553106212425, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648113, Length=514, Percent_Identity=25.2918287937743, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648441, Length=438, Percent_Identity=25.7990867579909, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24648439, Length=438, Percent_Identity=25.7990867579909, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 54668; Mature: 54537

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADPTLVELSANELRHRIGTGELSPVELLQACIDRIEAVNPYVNAVTATCYDRARAEAQ
CCCCCCEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AAERAVRAGEPLGLLHGLPMGVKDLEATAGLLTTYGSPIYRENIPAQDNVLVARLRAAGA
HHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHCCE
IVTGKTNIPEMGAGANSRNVVWGATGNPFDPRLNAGGSSGGSAAALACDMLPVCTGSDTG
EEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCEEECCCCCC
GSLRIPAAKCGVVGFRPSPGVVPSSRKLLGWTPISVVGPMGRTMAEACLQLAASAGLSAG
CEEECCHHHCCEEECCCCCCCCCCCCCEEECCCHHHHCCCHHHHHHHHHHHHHHCCCCCC
DPLTYPLDPHSFLHLEPADLGGLRVGYTEDFGACAVDDGIRAVFRAKIGAMRHLFRSCEE
CCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
IRLDLGDVHRCFDVLRAESFLAGMREAYERDPASLGPNTRANYEMGAAMSLLDSAWAQAE
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QTRILARFQEAFANFDVILAPTTPVSPFPWTALYASHINGEPQANYYRWLALTYVTTLTT
HHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
HPALSVPCGRDTAGMPFGLQVVGRFRDDHRLLSIGLGLEQAFARIDGLQRPRPDLARLGP
CCCEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHCCC
VQPALTSIVTHAPDPDDARGVPRLGARGTAEQASAV
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
TADPTLVELSANELRHRIGTGELSPVELLQACIDRIEAVNPYVNAVTATCYDRARAEAQ
CCCCCEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AAERAVRAGEPLGLLHGLPMGVKDLEATAGLLTTYGSPIYRENIPAQDNVLVARLRAAGA
HHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHCCE
IVTGKTNIPEMGAGANSRNVVWGATGNPFDPRLNAGGSSGGSAAALACDMLPVCTGSDTG
EEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCEEECCCCCC
GSLRIPAAKCGVVGFRPSPGVVPSSRKLLGWTPISVVGPMGRTMAEACLQLAASAGLSAG
CEEECCHHHCCEEECCCCCCCCCCCCCEEECCCHHHHCCCHHHHHHHHHHHHHHCCCCCC
DPLTYPLDPHSFLHLEPADLGGLRVGYTEDFGACAVDDGIRAVFRAKIGAMRHLFRSCEE
CCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
IRLDLGDVHRCFDVLRAESFLAGMREAYERDPASLGPNTRANYEMGAAMSLLDSAWAQAE
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QTRILARFQEAFANFDVILAPTTPVSPFPWTALYASHINGEPQANYYRWLALTYVTTLTT
HHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
HPALSVPCGRDTAGMPFGLQVVGRFRDDHRLLSIGLGLEQAFARIDGLQRPRPDLARLGP
CCCEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHCCC
VQPALTSIVTHAPDPDDARGVPRLGARGTAEQASAV
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA