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The map label for this gene is aceA [H]

Identifier: 222110645

GI number: 222110645

Start: 1513813

End: 1515144

Strand: Direct

Name: aceA [H]

Synonym: Dtpsy_1447

Alternate gene names: 222110645

Gene position: 1513813-1515144 (Clockwise)

Preceding gene: 222110643

Following gene: 222110646

Centisome position: 39.87

GC content: 62.54

Gene sequence:

>1332_bases
ATGCCAGATTCCCTGAACGCACAACTGAGCCGTGAGCAGCAAATCGCAGCCCTGGAAAAGGACTGGGCCCAGAATCCCCG
CTGGAAGAGCGTGAAGCGCGGCTACACAGCCGCCGACGTGGTGCGTCTGCGTGGCAGCCTCCAGCCCGAATACACGCTGG
CCAAGCGTGGCGCAGAGAAGCTGTGGGACAAGATCAATGGCGGCGCCAAGAAGGGCTATGTGAATGCCTTCGGTGCCATC
AGCGCCGGCCAAGCCATGCAGCAAGCCAAGGCGGGCCTGGAAGCCGTGTACCTGTCGGGCTGGCAGGTGGCCGCTGACGG
CAATACCAGCGAGACCATGTACCCCGACCAGTCGCTGTATGCCTATGACTCGGTGCCCACGATGGTGCGCCGCATCAACA
ACACCTTCAAGCGTGCCGACGAGATCCAGTGGGGCCGCGGTATCAATCCCGGCGACAAGGAATTCATCGACTACTTCCTG
CCCATCGTGGCCGACGCCGAAGCCGGCTTCGGCGGTGTGCTGAACGCCTTCGAACTGATGAAGAACATGATTGCCGCTGG
CGCGGCCGGCGTGCACTTCGAAGACCAACTGGCTGCCGTGAAGAAGTGCGGGCACATGGGCGGCAAGGTCCTCGTGCCCA
CACAGGAAGCCATCGAAAAGCTGATCGCTGCCCGCTTCGCTGCCGACGTGATGGGCGTGCCCACCATCGTGCTGGCCCGC
ACCGATGCCGAGGCCGCCAACCTGATCACCAGCGACCACGATGCCAACGACAAGCCGTTCTTGACGGGCGAGCGTACGCA
GGAAGGCTTCTATCGCGTGAAGAATGGTCTGGAGCAAGCCATCAGCCGCGGTGTGGCCTACGCCCCCTACGCCGACCTGG
TGTGGTGCGAGACCGGCACGCCCGACCTGGGTTTTGCCCGCGAGTTCGCCCAGGCCGTGCATGCGAAGTGCCCCGGCAAG
CTGCTGTCGTACAACTGCTCGCCGTCCTTCAACTGGAAGAAGAACCTGGACGAGAAGACCATCGCCAAGTTCCAGGACGA
GCTGTCCGCACTCGGCTACAAGTACCAGTTCATCACGCTGGCCGGCATCCACATCAACTGGTACAACACGTTCCAGTTCG
CCCATGCTTATGCCCGCGGCGAAGGCATGAAGCACTACGTGAACATGGTGCAGGAGCCCGAGTTCGCCGCACGTGAGCAG
GGCTACACCTTCGTGTCGCACCAGCAGGAGGTGGGCGCAGGCTATTTCGACGACGTGACCACCGTGATCCAGGGTGGTTC
TTCGAGCGTGAAGGCGCTGACCGGCTCCACCGAAGAAGAGCAGTTCCACTGA

Upstream 100 bases:

>100_bases
AAGACATAAGAGATTGATGTGGTCTTATAGAAGACTTAAAGTTGTGTCCAAGGGTCCCGGGAGCGGTACCCGCCCATTCC
TTCATCTCTTGGAGAGCAAC

Downstream 100 bases:

>100_bases
TCTTCTTGGTTGATGCGAGAAGCCCGGGGCCCCCGCCTCGGGCTTTTCTATTGCTGCAGGGTAAAATGCCACATCTGTTC
ACCAACAAGGGCGAGAAAGG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MPDSLNAQLSREQQIAALEKDWAQNPRWKSVKRGYTAADVVRLRGSLQPEYTLAKRGAEKLWDKINGGAKKGYVNAFGAI
SAGQAMQQAKAGLEAVYLSGWQVAADGNTSETMYPDQSLYAYDSVPTMVRRINNTFKRADEIQWGRGINPGDKEFIDYFL
PIVADAEAGFGGVLNAFELMKNMIAAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIEKLIAARFAADVMGVPTIVLAR
TDAEAANLITSDHDANDKPFLTGERTQEGFYRVKNGLEQAISRGVAYAPYADLVWCETGTPDLGFAREFAQAVHAKCPGK
LLSYNCSPSFNWKKNLDEKTIAKFQDELSALGYKYQFITLAGIHINWYNTFQFAHAYARGEGMKHYVNMVQEPEFAAREQ
GYTFVSHQQEVGAGYFDDVTTVIQGGSSSVKALTGSTEEEQFH

Sequences:

>Translated_443_residues
MPDSLNAQLSREQQIAALEKDWAQNPRWKSVKRGYTAADVVRLRGSLQPEYTLAKRGAEKLWDKINGGAKKGYVNAFGAI
SAGQAMQQAKAGLEAVYLSGWQVAADGNTSETMYPDQSLYAYDSVPTMVRRINNTFKRADEIQWGRGINPGDKEFIDYFL
PIVADAEAGFGGVLNAFELMKNMIAAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIEKLIAARFAADVMGVPTIVLAR
TDAEAANLITSDHDANDKPFLTGERTQEGFYRVKNGLEQAISRGVAYAPYADLVWCETGTPDLGFAREFAQAVHAKCPGK
LLSYNCSPSFNWKKNLDEKTIAKFQDELSALGYKYQFITLAGIHINWYNTFQFAHAYARGEGMKHYVNMVQEPEFAAREQ
GYTFVSHQQEVGAGYFDDVTTVIQGGSSSVKALTGSTEEEQFH
>Mature_442_residues
PDSLNAQLSREQQIAALEKDWAQNPRWKSVKRGYTAADVVRLRGSLQPEYTLAKRGAEKLWDKINGGAKKGYVNAFGAIS
AGQAMQQAKAGLEAVYLSGWQVAADGNTSETMYPDQSLYAYDSVPTMVRRINNTFKRADEIQWGRGINPGDKEFIDYFLP
IVADAEAGFGGVLNAFELMKNMIAAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIEKLIAARFAADVMGVPTIVLART
DAEAANLITSDHDANDKPFLTGERTQEGFYRVKNGLEQAISRGVAYAPYADLVWCETGTPDLGFAREFAQAVHAKCPGKL
LSYNCSPSFNWKKNLDEKTIAKFQDELSALGYKYQFITLAGIHINWYNTFQFAHAYARGEGMKHYVNMVQEPEFAAREQG
YTFVSHQQEVGAGYFDDVTTVIQGGSSSVKALTGSTEEEQFH

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=433, Percent_Identity=72.7482678983834, Blast_Score=675, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=265, Percent_Identity=29.811320754717, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71982926, Length=423, Percent_Identity=54.8463356973995, Blast_Score=459, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17561814, Length=423, Percent_Identity=54.8463356973995, Blast_Score=459, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320908, Length=291, Percent_Identity=37.8006872852234, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6325263, Length=250, Percent_Identity=35.6, Blast_Score=154, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48727; Mature: 48596

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSLNAQLSREQQIAALEKDWAQNPRWKSVKRGYTAADVVRLRGSLQPEYTLAKRGAEK
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHH
LWDKINGGAKKGYVNAFGAISAGQAMQQAKAGLEAVYLSGWQVAADGNTSETMYPDQSLY
HHHHHCCCCCCCCHHHHCCHHHHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCEE
AYDSVPTMVRRINNTFKRADEIQWGRGINPGDKEFIDYFLPIVADAEAGFGGVLNAFELM
EHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KNMIAAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIEKLIAARFAADVMGVPTIVLAR
HHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEE
TDAEAANLITSDHDANDKPFLTGERTQEGFYRVKNGLEQAISRGVAYAPYADLVWCETGT
CCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCCC
PDLGFAREFAQAVHAKCPGKLLSYNCSPSFNWKKNLDEKTIAKFQDELSALGYKYQFITL
CCHHHHHHHHHHHHHCCCCHHEEECCCCCCCHHCCCCHHHHHHHHHHHHHCCCEEEEEEE
AGIHINWYNTFQFAHAYARGEGMKHYVNMVQEPEFAAREQGYTFVSHQQEVGAGYFDDVT
EEEEEEEEHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCEEECCHHHHCCCHHHHHH
TVIQGGSSSVKALTGSTEEEQFH
HHHCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
PDSLNAQLSREQQIAALEKDWAQNPRWKSVKRGYTAADVVRLRGSLQPEYTLAKRGAEK
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHH
LWDKINGGAKKGYVNAFGAISAGQAMQQAKAGLEAVYLSGWQVAADGNTSETMYPDQSLY
HHHHHCCCCCCCCHHHHCCHHHHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCEE
AYDSVPTMVRRINNTFKRADEIQWGRGINPGDKEFIDYFLPIVADAEAGFGGVLNAFELM
EHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KNMIAAGAAGVHFEDQLAAVKKCGHMGGKVLVPTQEAIEKLIAARFAADVMGVPTIVLAR
HHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEE
TDAEAANLITSDHDANDKPFLTGERTQEGFYRVKNGLEQAISRGVAYAPYADLVWCETGT
CCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCCC
PDLGFAREFAQAVHAKCPGKLLSYNCSPSFNWKKNLDEKTIAKFQDELSALGYKYQFITL
CCHHHHHHHHHHHHHCCCCHHEEECCCCCCCHHCCCCHHHHHHHHHHHHHCCCEEEEEEE
AGIHINWYNTFQFAHAYARGEGMKHYVNMVQEPEFAAREQGYTFVSHQQEVGAGYFDDVT
EEEEEEEEHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCEEECCHHHHCCCHHHHHH
TVIQGGSSSVKALTGSTEEEQFH
HHHCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]