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The map label for this gene is oprM [H]

Identifier: 222110363

GI number: 222110363

Start: 1216923

End: 1218428

Strand: Direct

Name: oprM [H]

Synonym: Dtpsy_1154

Alternate gene names: 222110363

Gene position: 1216923-1218428 (Clockwise)

Preceding gene: 222110362

Following gene: 222110364

Centisome position: 32.05

GC content: 68.39

Gene sequence:

>1506_bases
ATGAAAACATCTTTCGCATTCACACGACCCGCCAGGACGCTGGCGCCGCTCGCCCTCGCGGCGGCGCTGGCTGGCTGCTC
CATGGCACCGAAGTATGACCGGCCCGCAGCGCCGATCGACACGGCCTATCCCTCGGGCGCGGCCTATGTGGAACTGGCGG
CCGCGACGCCCGACGACGCGATCACGGCCGAGATCGGTTGGCGGGACTTCTTCCGCGACCCGCTGCTGCAGCAGTTGATC
GGCATTTCGCTGGAGAACAACCGCGACATGCACAAAGCCGCGCTCAACGTGGAGGCGGCTCAGGCGCTGTACCGTATCCA
GCGTGCCGAGATGCTGCCGAACCTGGGCGTTTCCGCCCGCGGCGCGTCAGAGCGCGTACCGGCCGACCTCAGTACCACGG
GGCAAAGCGACGTGCTCCGGCGCTACGACGTGGCCGGGGTGACGGCCGCCTGGGAGCTGGACCTGTGGGGCCGCATCCGC
AGCCTCAACGACCGGGCGCTGGCGTCCTACCTGGCCCTCGACGAGACCCGCATCGCCACGCAGATGAGCCTGGTGTCCGA
GGTGGCCAGCGCCTACCTCACGCTGCGCGCCGACCAGGAACTGCTGCGCCTGACGAGCGACACCCTCGCCACACAGAAGC
GCTCCTATGACCTGACCACCCAACTGGTGGAAGCGGGAAATTCCACGCAGCTCGACCTGCGCCGCGCGGAGATCGCGCTG
CGCACCGCCGAGGCCAACCGCGCCGCCTACACGCGGCAGGCGGCCAAGGACCGCAACGCGCTGGTGCTGCTGCTGGGCCA
GCCGCTGACGCCGGAGCTGTCCCGGCAGCTTGACGAGGCCGTGGCGCTGCCGGACGACATCGTGCCGACCGACCTGCCGA
GCGGCCTGCCGTCCGAACTGCTCGCGCGTCGGCCGGACATCCGCGCCGCCGAGCAGATGCTGATCGGCGCCAACGCCAAC
ATCGGCGCGGCGCGGGCCGCGTTCTTCCCGACGATTAGCCTCACGGGCTCGGCGGGCACGGCCAGCGCTTCGCTGGACGG
CCTGTTCGATTCGGGCTCGCGGGCCTGGAGCTTCCTGCCGCAGATCACGCTGCCCATCTTCCGCGGCGGCGCGTTGCGAG
CCAATCTGGACGTGGCACAGGTACAAAAGAGGATCGAAATCGCCAACTACGAGAAGTCTATCCAGGCGGCCTTTGCTGAG
GTGGCTGACGGCTTGGCGGGCAAGCGCACGCTTGATGAGCAGATCCGCTCGGAGCAGCTCCTGGTAGCGGCTAGCCAGAA
AGCCTACCAACTAGCCGAGCAACGCTTCCAGGAAGGCGTGGACGACAACCTCACCTTGCTCGATGCGCAACGCACACAGT
ACGGTGCGCAGCAGACCCTGGTGCGCACGCGCCTGACGCGGCTGAACAACCTCATCCACCTCTACAAGGCGCTGGGTGGC
GGCTGGACTGAGCACACGGTGCAATCGGGCGCCACCGGGCAACCATCCGCCCGGTCGCCGGGATAA

Upstream 100 bases:

>100_bases
TCTACGTTGTGGTGCGCAAGCTGGCGCTGCGCCGCGAAGCGCGCCATGCCCGTGGCGGCATGACCGACCAGCACGCATGA
CGGCCAGGAGGACATACGAC

Downstream 100 bases:

>100_bases
GGGCTATGCGCAACGGCGGCGTTCCCCATCGTTGCGCACGGAGAACCGGATGAGGATCGGTGAACTTGCGCGCTTGACGG
GCACCCCCCCGGAGAACATT

Product: NodT family RND efflux system, outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MKTSFAFTRPARTLAPLALAAALAGCSMAPKYDRPAAPIDTAYPSGAAYVELAAATPDDAITAEIGWRDFFRDPLLQQLI
GISLENNRDMHKAALNVEAAQALYRIQRAEMLPNLGVSARGASERVPADLSTTGQSDVLRRYDVAGVTAAWELDLWGRIR
SLNDRALASYLALDETRIATQMSLVSEVASAYLTLRADQELLRLTSDTLATQKRSYDLTTQLVEAGNSTQLDLRRAEIAL
RTAEANRAAYTRQAAKDRNALVLLLGQPLTPELSRQLDEAVALPDDIVPTDLPSGLPSELLARRPDIRAAEQMLIGANAN
IGAARAAFFPTISLTGSAGTASASLDGLFDSGSRAWSFLPQITLPIFRGGALRANLDVAQVQKRIEIANYEKSIQAAFAE
VADGLAGKRTLDEQIRSEQLLVAASQKAYQLAEQRFQEGVDDNLTLLDAQRTQYGAQQTLVRTRLTRLNNLIHLYKALGG
GWTEHTVQSGATGQPSARSPG

Sequences:

>Translated_501_residues
MKTSFAFTRPARTLAPLALAAALAGCSMAPKYDRPAAPIDTAYPSGAAYVELAAATPDDAITAEIGWRDFFRDPLLQQLI
GISLENNRDMHKAALNVEAAQALYRIQRAEMLPNLGVSARGASERVPADLSTTGQSDVLRRYDVAGVTAAWELDLWGRIR
SLNDRALASYLALDETRIATQMSLVSEVASAYLTLRADQELLRLTSDTLATQKRSYDLTTQLVEAGNSTQLDLRRAEIAL
RTAEANRAAYTRQAAKDRNALVLLLGQPLTPELSRQLDEAVALPDDIVPTDLPSGLPSELLARRPDIRAAEQMLIGANAN
IGAARAAFFPTISLTGSAGTASASLDGLFDSGSRAWSFLPQITLPIFRGGALRANLDVAQVQKRIEIANYEKSIQAAFAE
VADGLAGKRTLDEQIRSEQLLVAASQKAYQLAEQRFQEGVDDNLTLLDAQRTQYGAQQTLVRTRLTRLNNLIHLYKALGG
GWTEHTVQSGATGQPSARSPG
>Mature_501_residues
MKTSFAFTRPARTLAPLALAAALAGCSMAPKYDRPAAPIDTAYPSGAAYVELAAATPDDAITAEIGWRDFFRDPLLQQLI
GISLENNRDMHKAALNVEAAQALYRIQRAEMLPNLGVSARGASERVPADLSTTGQSDVLRRYDVAGVTAAWELDLWGRIR
SLNDRALASYLALDETRIATQMSLVSEVASAYLTLRADQELLRLTSDTLATQKRSYDLTTQLVEAGNSTQLDLRRAEIAL
RTAEANRAAYTRQAAKDRNALVLLLGQPLTPELSRQLDEAVALPDDIVPTDLPSGLPSELLARRPDIRAAEQMLIGANAN
IGAARAAFFPTISLTGSAGTASASLDGLFDSGSRAWSFLPQITLPIFRGGALRANLDVAQVQKRIEIANYEKSIQAAFAE
VADGLAGKRTLDEQIRSEQLLVAASQKAYQLAEQRFQEGVDDNLTLLDAQRTQYGAQQTLVRTRLTRLNNLIHLYKALGG
GWTEHTVQSGATGQPSARSPG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=474, Percent_Identity=37.5527426160338, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI2367348, Length=472, Percent_Identity=27.9661016949153, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54127; Mature: 54127

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSFAFTRPARTLAPLALAAALAGCSMAPKYDRPAAPIDTAYPSGAAYVELAAATPDDA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
ITAEIGWRDFFRDPLLQQLIGISLENNRDMHKAALNVEAAQALYRIQRAEMLPNLGVSAR
EEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GASERVPADLSTTGQSDVLRRYDVAGVTAAWELDLWGRIRSLNDRALASYLALDETRIAT
CCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHH
QMSLVSEVASAYLTLRADQELLRLTSDTLATQKRSYDLTTQLVEAGNSTQLDLRRAEIAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEHHHHHHHHH
RTAEANRAAYTRQAAKDRNALVLLLGQPLTPELSRQLDEAVALPDDIVPTDLPSGLPSEL
HHHCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
LARRPDIRAAEQMLIGANANIGAARAAFFPTISLTGSAGTASASLDGLFDSGSRAWSFLP
HHCCCCHHHHHHHHCCCCCCCCHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
QITLPIFRGGALRANLDVAQVQKRIEIANYEKSIQAAFAEVADGLAGKRTLDEQIRSEQL
HHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHE
LVAASQKAYQLAEQRFQEGVDDNLTLLDAQRTQYGAQQTLVRTRLTRLNNLIHLYKALGG
EEHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GWTEHTVQSGATGQPSARSPG
CCHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTSFAFTRPARTLAPLALAAALAGCSMAPKYDRPAAPIDTAYPSGAAYVELAAATPDDA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
ITAEIGWRDFFRDPLLQQLIGISLENNRDMHKAALNVEAAQALYRIQRAEMLPNLGVSAR
EEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GASERVPADLSTTGQSDVLRRYDVAGVTAAWELDLWGRIRSLNDRALASYLALDETRIAT
CCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHH
QMSLVSEVASAYLTLRADQELLRLTSDTLATQKRSYDLTTQLVEAGNSTQLDLRRAEIAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEHHHHHHHHH
RTAEANRAAYTRQAAKDRNALVLLLGQPLTPELSRQLDEAVALPDDIVPTDLPSGLPSEL
HHHCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
LARRPDIRAAEQMLIGANANIGAARAAFFPTISLTGSAGTASASLDGLFDSGSRAWSFLP
HHCCCCHHHHHHHHCCCCCCCCHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
QITLPIFRGGALRANLDVAQVQKRIEIANYEKSIQAAFAEVADGLAGKRTLDEQIRSEQL
HHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHE
LVAASQKAYQLAEQRFQEGVDDNLTLLDAQRTQYGAQQTLVRTRLTRLNNLIHLYKALGG
EEHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GWTEHTVQSGATGQPSARSPG
CCHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]