Click here to switch to the map view.

The map label for this gene is rluB [H]

Identifier: 222110298

GI number: 222110298

Start: 1132770

End: 1134077

Strand: Direct

Name: rluB [H]

Synonym: Dtpsy_1085

Alternate gene names: 222110298

Gene position: 1132770-1134077 (Clockwise)

Preceding gene: 222110297

Following gene: 222110299

Centisome position: 29.84

GC content: 64.6

Gene sequence:

>1308_bases
ATGGCGGGTCACTCGCCTTTGCCCGCATCGGAGGCTGCTGCGCCGCGCGGGCCCCGCAAGCGCAAGTCTGCGGAAGGCGC
GCTTCAGGAGAATGTGGCAGTGCCGCAACTGAGCGCTGACGGTGCCCAATCGGACGTGAAGATGCAAGAAGTGCATGAGC
AAGGCGCAGCTGTTGAGGTGCCACGACAGCCCAGCGGCCATGCCGCCAAGCGCGCGCCGAAGGGCGATGGCTATCAGTTT
GCCGACGTGGTGTCGGGTCAGTTCGACGAGGATGAAGCCAACGCCGAGCTCGCCCCCGCCAAGCGGGTGCTGCTTCCCCA
GCCAGAAACCCCCAAACTTCACAAGGTGCTTGCCCAGGCGGGCATGGGGTCTCGGCTGGAAATGGAGCAGCTCATCCTGG
AAGGGCGTATTTCCGTCAACAACGAGCCGGCCCATATCGGACAGCGCGTGCAGTTTGGGGACCAGATAAAGGTCAACGGC
AAACCCATCCGCTTCCGTATTGCACCACCGCCGGTACGCGTGATTGCATACCACAAGCCGGTGGGCGAAGTGGTTACGCA
TGACGATCCGCAAAACCGCCCCACGGTGTTTCGCAAACTACCCCGGCTGCAGCAGGGCAAATGGCAATCCGTCGGGCGTC
TGGACCTGAATACCGAAGGTTTGTTGCTATTTACCAGTTCGGGGGAGCTTGCGAACAAGTTGATGCACCCCCGCTTCGGG
CTGGAGCGTGAATATGCGGCCCGCGTGCTGGGTGCGTTGAGCACCGAGGAAAAGCAGCGCTTGCTGGAAGGCGTGCCGCT
GGAGGATGGCATGGCTTCCTTTGGCTCCGTGGAAGACGGCGGCGGCGAGGGCGCCAACTGTTGGTACCGGGTCACCATCT
CTGAAGGCCGTAACCGCGAGGTCCGCCGTATGTTCGAGGCGGTAGGACATGCGGTGAGCCGGCTGATTCGGATTCGCTAT
GGGGCCATGATGCTGCCGCGCGGGCTCAAGCGCGGGGCGTGGCTGGAGCTTGATGAGCGCGACATTCGTGCCCTGATGCA
GGCGGCAGGGGCAACGCTGGATGGTGGTGGTGCGCGCCCCACTAGTGGCCAGGGCAGGGGCGGCCGCAAAGGCGATCGTC
GATCCGCGGGGCCCGGCAAGGCATCTGGCAGTGGGCGTGACAAGACAGGGGGGCGTGCGGGGCGATCGCCCTCACCGAAT
GCCCAGCCGGACCCGATGAAGACGTCGCTTGGCTATATCGGGGCCGACAGTTTCTCGCGCCATCGCCAACAGCAACGCAA
CAACGGGCCGCGCCGCGGCGGCCGTTAG

Upstream 100 bases:

>100_bases
ATGCCCCTGATCTGGTAGTGGGCGCGACACACAGTGGTGCCGAAGAGGGGCCACGGGACATTGACATATGAATGATGAAT
CTCACAAGGATCCGGACTCC

Downstream 100 bases:

>100_bases
GGGATTGGCGTAAAGCTAAAATTGATGGTTTGCCTTCAGGGCAAAAATACCGCAACACCTCACTAGGAAAATCAACATGG
CTATCGAACGCACCCTTTCC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MAGHSPLPASEAAAPRGPRKRKSAEGALQENVAVPQLSADGAQSDVKMQEVHEQGAAVEVPRQPSGHAAKRAPKGDGYQF
ADVVSGQFDEDEANAELAPAKRVLLPQPETPKLHKVLAQAGMGSRLEMEQLILEGRISVNNEPAHIGQRVQFGDQIKVNG
KPIRFRIAPPPVRVIAYHKPVGEVVTHDDPQNRPTVFRKLPRLQQGKWQSVGRLDLNTEGLLLFTSSGELANKLMHPRFG
LEREYAARVLGALSTEEKQRLLEGVPLEDGMASFGSVEDGGGEGANCWYRVTISEGRNREVRRMFEAVGHAVSRLIRIRY
GAMMLPRGLKRGAWLELDERDIRALMQAAGATLDGGGARPTSGQGRGGRKGDRRSAGPGKASGSGRDKTGGRAGRSPSPN
AQPDPMKTSLGYIGADSFSRHRQQQRNNGPRRGGR

Sequences:

>Translated_435_residues
MAGHSPLPASEAAAPRGPRKRKSAEGALQENVAVPQLSADGAQSDVKMQEVHEQGAAVEVPRQPSGHAAKRAPKGDGYQF
ADVVSGQFDEDEANAELAPAKRVLLPQPETPKLHKVLAQAGMGSRLEMEQLILEGRISVNNEPAHIGQRVQFGDQIKVNG
KPIRFRIAPPPVRVIAYHKPVGEVVTHDDPQNRPTVFRKLPRLQQGKWQSVGRLDLNTEGLLLFTSSGELANKLMHPRFG
LEREYAARVLGALSTEEKQRLLEGVPLEDGMASFGSVEDGGGEGANCWYRVTISEGRNREVRRMFEAVGHAVSRLIRIRY
GAMMLPRGLKRGAWLELDERDIRALMQAAGATLDGGGARPTSGQGRGGRKGDRRSAGPGKASGSGRDKTGGRAGRSPSPN
AQPDPMKTSLGYIGADSFSRHRQQQRNNGPRRGGR
>Mature_434_residues
AGHSPLPASEAAAPRGPRKRKSAEGALQENVAVPQLSADGAQSDVKMQEVHEQGAAVEVPRQPSGHAAKRAPKGDGYQFA
DVVSGQFDEDEANAELAPAKRVLLPQPETPKLHKVLAQAGMGSRLEMEQLILEGRISVNNEPAHIGQRVQFGDQIKVNGK
PIRFRIAPPPVRVIAYHKPVGEVVTHDDPQNRPTVFRKLPRLQQGKWQSVGRLDLNTEGLLLFTSSGELANKLMHPRFGL
EREYAARVLGALSTEEKQRLLEGVPLEDGMASFGSVEDGGGEGANCWYRVTISEGRNREVRRMFEAVGHAVSRLIRIRYG
AMMLPRGLKRGAWLELDERDIRALMQAAGATLDGGGARPTSGQGRGGRKGDRRSAGPGKASGSGRDKTGGRAGRSPSPNA
QPDPMKTSLGYIGADSFSRHRQQQRNNGPRRGGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=232, Percent_Identity=55.1724137931034, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1790453, Length=300, Percent_Identity=29.3333333333333, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788510, Length=213, Percent_Identity=30.0469483568075, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081838, Length=184, Percent_Identity=31.5217391304348, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 47046; Mature: 46915

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGHSPLPASEAAAPRGPRKRKSAEGALQENVAVPQLSADGAQSDVKMQEVHEQGAAVEV
CCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEC
PRQPSGHAAKRAPKGDGYQFADVVSGQFDEDEANAELAPAKRVLLPQPETPKLHKVLAQA
CCCCCCCHHHCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCHHEECCCCCCHHHHHHHHHH
GMGSRLEMEQLILEGRISVNNEPAHIGQRVQFGDQIKVNGKPIRFRIAPPPVRVIAYHKP
CCCCCHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEECCCEEEEEECCCCEEEEEECCC
VGEVVTHDDPQNRPTVFRKLPRLQQGKWQSVGRLDLNTEGLLLFTSSGELANKLMHPRFG
CCHHHCCCCCCCCCHHHHHCCHHHCCCCCCCCEEECCCCCEEEEECCCHHHHHHHCCCCC
LEREYAARVLGALSTEEKQRLLEGVPLEDGMASFGSVEDGGGEGANCWYRVTISEGRNRE
CCHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCHH
VRRMFEAVGHAVSRLIRIRYGAMMLPRGLKRGAWLELDERDIRALMQAAGATLDGGGARP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHCCEECCCCCCC
TSGQGRGGRKGDRRSAGPGKASGSGRDKTGGRAGRSPSPNAQPDPMKTSLGYIGADSFSR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHH
HRQQQRNNGPRRGGR
HHHHHHCCCCCCCCC
>Mature Secondary Structure 
AGHSPLPASEAAAPRGPRKRKSAEGALQENVAVPQLSADGAQSDVKMQEVHEQGAAVEV
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEC
PRQPSGHAAKRAPKGDGYQFADVVSGQFDEDEANAELAPAKRVLLPQPETPKLHKVLAQA
CCCCCCCHHHCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCHHEECCCCCCHHHHHHHHHH
GMGSRLEMEQLILEGRISVNNEPAHIGQRVQFGDQIKVNGKPIRFRIAPPPVRVIAYHKP
CCCCCHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEECCCEEEEEECCCCEEEEEECCC
VGEVVTHDDPQNRPTVFRKLPRLQQGKWQSVGRLDLNTEGLLLFTSSGELANKLMHPRFG
CCHHHCCCCCCCCCHHHHHCCHHHCCCCCCCCEEECCCCCEEEEECCCHHHHHHHCCCCC
LEREYAARVLGALSTEEKQRLLEGVPLEDGMASFGSVEDGGGEGANCWYRVTISEGRNRE
CCHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCEEEEEEECCCCCHH
VRRMFEAVGHAVSRLIRIRYGAMMLPRGLKRGAWLELDERDIRALMQAAGATLDGGGARP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHCCEECCCCCCC
TSGQGRGGRKGDRRSAGPGKASGSGRDKTGGRAGRSPSPNAQPDPMKTSLGYIGADSFSR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHH
HRQQQRNNGPRRGGR
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]