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The map label for this gene is trmJ [H]

Identifier: 222110178

GI number: 222110178

Start: 1001102

End: 1001896

Strand: Direct

Name: trmJ [H]

Synonym: Dtpsy_0964

Alternate gene names: 222110178

Gene position: 1001102-1001896 (Clockwise)

Preceding gene: 222110176

Following gene: 222110179

Centisome position: 26.37

GC content: 66.29

Gene sequence:

>795_bases
ATGAGAACCCGCTTCATCCTGATCCAGACCAGCCACGCCGGCAACGTGGGCGCCACGGCCCGCGCCATGAAGACCATGGG
TTTCGATGACCTGGTGCTGGTCGCACCGCGCTGGCCCAACGTGCTGCGCAAGGAGGAGACCATCCAGCGGGCCAGCGGCG
CGCTGGACGTGCTGGCCCATGCCCGCATCGTCGCCACGCTGGACGAGGCGCTGGAGGGCGTGAGCCACCTGTGTGCCACG
GCGATGACGCCGCGCGACTTCGGCCCGCCCACGCGCACGCCGCGCCAGCATTTCGAGATGCTATTGAATAATGAGCTGTC
AGCGCTTGATGGCAAAGCGCCAGGGGCTGATTTGGCTAGAAAACCGTCGCCGGAATCCGGCGTGGCATTCCTGTTCGGCT
CCGAGCGTTTTGGCATGGCCAATGAGGATGTGTACCGCTGCCATGTGGCGCTGAGCATTCCCACCAATCCGCAGTTCGGC
TCGCTCAACCTGGGCGCGGCGGTACAGGTCATCGCCTATGAATGGCGGCAGGCCCTTGCCACCCGTGGGGAGGGCTTTGC
GGTGCAGGAGTCCGCCCCTCAGGGCGAGCGCGCGGACGCCGCGCAGGTGGCCGGCATGCTGGCGCACTGGGAGCAGGCGC
TCACGGGCATCGGCTTTCTGGACCCGGCCGCGCCCAAGAAGCTCATGCCGCGCTTGAACCAGCTTTTCAACCGCGCGCAA
CTCACGCAGGAGGAAATCCACATCCTGCGCGGTGTCGCCAAATCCATGCTGCAGGCCACGCAGGCAAACCGCTAG

Upstream 100 bases:

>100_bases
GGCATTTTACCCATCACCCTCCGGGCTTGCACGCGCGGGCCTTCGGGCGCTTTGCAGCAGCGGCGACAATGGCAGGCTTG
CTCCCGAGTCACTTCGCGCC

Downstream 100 bases:

>100_bases
ACTGCCAAGCTTCTCGTTACACCATTGCCCTTCATGCTTGCCCGCCTGCGATCTGACGTCCAGTGCATCCTCGAACGCGA
CCCGGCTGCGCGCAGCACCT

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRTRFILIQTSHAGNVGATARAMKTMGFDDLVLVAPRWPNVLRKEETIQRASGALDVLAHARIVATLDEALEGVSHLCAT
AMTPRDFGPPTRTPRQHFEMLLNNELSALDGKAPGADLARKPSPESGVAFLFGSERFGMANEDVYRCHVALSIPTNPQFG
SLNLGAAVQVIAYEWRQALATRGEGFAVQESAPQGERADAAQVAGMLAHWEQALTGIGFLDPAAPKKLMPRLNQLFNRAQ
LTQEEIHILRGVAKSMLQATQANR

Sequences:

>Translated_264_residues
MRTRFILIQTSHAGNVGATARAMKTMGFDDLVLVAPRWPNVLRKEETIQRASGALDVLAHARIVATLDEALEGVSHLCAT
AMTPRDFGPPTRTPRQHFEMLLNNELSALDGKAPGADLARKPSPESGVAFLFGSERFGMANEDVYRCHVALSIPTNPQFG
SLNLGAAVQVIAYEWRQALATRGEGFAVQESAPQGERADAAQVAGMLAHWEQALTGIGFLDPAAPKKLMPRLNQLFNRAQ
LTQEEIHILRGVAKSMLQATQANR
>Mature_264_residues
MRTRFILIQTSHAGNVGATARAMKTMGFDDLVLVAPRWPNVLRKEETIQRASGALDVLAHARIVATLDEALEGVSHLCAT
AMTPRDFGPPTRTPRQHFEMLLNNELSALDGKAPGADLARKPSPESGVAFLFGSERFGMANEDVYRCHVALSIPTNPQFG
SLNLGAAVQVIAYEWRQALATRGEGFAVQESAPQGERADAAQVAGMLAHWEQALTGIGFLDPAAPKKLMPRLNQLFNRAQ
LTQEEIHILRGVAKSMLQATQANR

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=263, Percent_Identity=39.9239543726236, Blast_Score=162, Evalue=1e-41,
Organism=Escherichia coli, GI1790865, Length=242, Percent_Identity=32.2314049586777, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28688; Mature: 28688

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRFILIQTSHAGNVGATARAMKTMGFDDLVLVAPRWPNVLRKEETIQRASGALDVLAH
CCCEEEEEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
ARIVATLDEALEGVSHLCATAMTPRDFGPPTRTPRQHFEMLLNNELSALDGKAPGADLAR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCC
KPSPESGVAFLFGSERFGMANEDVYRCHVALSIPTNPQFGSLNLGAAVQVIAYEWRQALA
CCCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
TRGEGFAVQESAPQGERADAAQVAGMLAHWEQALTGIGFLDPAAPKKLMPRLNQLFNRAQ
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
LTQEEIHILRGVAKSMLQATQANR
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTRFILIQTSHAGNVGATARAMKTMGFDDLVLVAPRWPNVLRKEETIQRASGALDVLAH
CCCEEEEEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
ARIVATLDEALEGVSHLCATAMTPRDFGPPTRTPRQHFEMLLNNELSALDGKAPGADLAR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCC
KPSPESGVAFLFGSERFGMANEDVYRCHVALSIPTNPQFGSLNLGAAVQVIAYEWRQALA
CCCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
TRGEGFAVQESAPQGERADAAQVAGMLAHWEQALTGIGFLDPAAPKKLMPRLNQLFNRAQ
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
LTQEEIHILRGVAKSMLQATQANR
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA