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The map label for this gene is rpfG [H]

Identifier: 222110176

GI number: 222110176

Start: 998747

End: 999880

Strand: Direct

Name: rpfG [H]

Synonym: Dtpsy_0962

Alternate gene names: 222110176

Gene position: 998747-999880 (Clockwise)

Preceding gene: 222110175

Following gene: 222110178

Centisome position: 26.31

GC content: 66.05

Gene sequence:

>1134_bases
ATGGATCAGCAACGCTACGTGAACGCCGCCCCGGACCGCCTGGTCGACAAGCCCATCGCCACCGTGCTGGTAGTGGACGA
CACGCCCGACAACCTGACGCTCATGGCCGGACTGCTGCAGGGGCATTACCGCGTCAAGGTGGCGGCCAGCGGTGAGAAGG
CCCTGGTAATCGCGCAGTCCGACGCTCCGCCCGACCTGCTGCTGCTGGACATCATGATGCCCGGCATCGACGGCTACGAG
GTCTGCCGGCGCCTCAAGGCCAACGAGGCCACGCGTCACATTCCGGTGATCTTTCTCACCGCGCGCTCCGATCCCGACGA
CGAGCGCGAAGGCCTGGCGCTGGGGGCGGTGGACTACATCACCAAGCCCATCAGCCCCTCCATCCTGCTGGCGCGCGTGG
CCACGCACCTGGCGCTCAAGGCCACGGCCGACTTCCTGCGCGACAAGAGCGCCTACCTGGAGCGCGAGGTGGCGATGCGC
ACGCTGGAGGTGCAGGCGATCCAGGACGTGACCATCATGGCGATGACTTCTCTGGCCGAGACGCGCGACAACGAAACCGG
CAACCACATCCGCCGCACGCAGCTGTACGTCAAGGCCCTGGCTGAGCGTCTGCGCCACCACCCGCGCTTCGAGGCGGCCT
TGAACGACCGCATGATCGACCTGCTGTACAAGTCGGCGCCGCTGCACGACATCGGCAAGATCGGCATCCCCGACAGCATC
CTGCTCAAGCCGGGCAAGCTCACGGTGGAGGAGTTCGAGATCATGAAGACCCACACCACGCTGGGGCGCAAGGCCATCGA
GGATGCCGAGCAGCGCCTGGGCATGCGCGTGGCCTTCCTCAGCGTGTCCAAGGAGATCGCGTACAGCCACCAGGAGAAGT
GGGACGGATCGGGCTACCCCGAAGGCCTCAAGGGCGACGCCATTCCCGTGTCTGCGCGCCTGATGGCCGTGGCCGACGTG
TATGACGCACTCATCAGCAAGCGCGTGTACAAGCCGGCGTTCTCCCACGAACAGGCCTGCACCATCATCCTCAAGGGGCG
GGGCACGCACTTCGACCCGGACATGGTGGATGCGTTCGTGGAACTGGCCGACGACTTCCGCGGCATCGCACAGCGCTACC
CCGATCCCCATTGA

Upstream 100 bases:

>100_bases
GCAGCTTCGCCTATGACGACGCGCTGCGGCGGCTGGAGGCATGCCCGGCGGCCCCGGCGGAATGACGCCCCATCCCACCA
ACTCTTTGGCAGGACTTCCC

Downstream 100 bases:

>100_bases
AGTGCTGCAGCAGTTCAAATAAAAATAGCTGCCATGGCGCTTGCAGCAAGCGCGGGCAGCTATTGTTTTATGAGTGTGAG
GATTACGGCGCTGCCGTGGG

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDQQRYVNAAPDRLVDKPIATVLVVDDTPDNLTLMAGLLQGHYRVKVAASGEKALVIAQSDAPPDLLLLDIMMPGIDGYE
VCRRLKANEATRHIPVIFLTARSDPDDEREGLALGAVDYITKPISPSILLARVATHLALKATADFLRDKSAYLEREVAMR
TLEVQAIQDVTIMAMTSLAETRDNETGNHIRRTQLYVKALAERLRHHPRFEAALNDRMIDLLYKSAPLHDIGKIGIPDSI
LLKPGKLTVEEFEIMKTHTTLGRKAIEDAEQRLGMRVAFLSVSKEIAYSHQEKWDGSGYPEGLKGDAIPVSARLMAVADV
YDALISKRVYKPAFSHEQACTIILKGRGTHFDPDMVDAFVELADDFRGIAQRYPDPH

Sequences:

>Translated_377_residues
MDQQRYVNAAPDRLVDKPIATVLVVDDTPDNLTLMAGLLQGHYRVKVAASGEKALVIAQSDAPPDLLLLDIMMPGIDGYE
VCRRLKANEATRHIPVIFLTARSDPDDEREGLALGAVDYITKPISPSILLARVATHLALKATADFLRDKSAYLEREVAMR
TLEVQAIQDVTIMAMTSLAETRDNETGNHIRRTQLYVKALAERLRHHPRFEAALNDRMIDLLYKSAPLHDIGKIGIPDSI
LLKPGKLTVEEFEIMKTHTTLGRKAIEDAEQRLGMRVAFLSVSKEIAYSHQEKWDGSGYPEGLKGDAIPVSARLMAVADV
YDALISKRVYKPAFSHEQACTIILKGRGTHFDPDMVDAFVELADDFRGIAQRYPDPH
>Mature_377_residues
MDQQRYVNAAPDRLVDKPIATVLVVDDTPDNLTLMAGLLQGHYRVKVAASGEKALVIAQSDAPPDLLLLDIMMPGIDGYE
VCRRLKANEATRHIPVIFLTARSDPDDEREGLALGAVDYITKPISPSILLARVATHLALKATADFLRDKSAYLEREVAMR
TLEVQAIQDVTIMAMTSLAETRDNETGNHIRRTQLYVKALAERLRHHPRFEAALNDRMIDLLYKSAPLHDIGKIGIPDSI
LLKPGKLTVEEFEIMKTHTTLGRKAIEDAEQRLGMRVAFLSVSKEIAYSHQEKWDGSGYPEGLKGDAIPVSARLMAVADV
YDALISKRVYKPAFSHEQACTIILKGRGTHFDPDMVDAFVELADDFRGIAQRYPDPH

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=112, Percent_Identity=40.1785714285714, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788394, Length=124, Percent_Identity=36.2903225806452, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=38.7931034482759, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790860, Length=120, Percent_Identity=41.6666666666667, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789809, Length=125, Percent_Identity=37.6, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786784, Length=116, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=37.1428571428571, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 41929; Mature: 41929

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQRYVNAAPDRLVDKPIATVLVVDDTPDNLTLMAGLLQGHYRVKVAASGEKALVIAQS
CCCCCCCCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEEEECCCEEEEEECC
DAPPDLLLLDIMMPGIDGYEVCRRLKANEATRHIPVIFLTARSDPDDEREGLALGAVDYI
CCCCCEEEEEHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHCCEEEEHHHHH
TKPISPSILLARVATHLALKATADFLRDKSAYLEREVAMRTLEVQAIQDVTIMAMTSLAE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRDNETGNHIRRTQLYVKALAERLRHHPRFEAALNDRMIDLLYKSAPLHDIGKIGIPDSI
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCE
LLKPGKLTVEEFEIMKTHTTLGRKAIEDAEQRLGMRVAFLSVSKEIAYSHQEKWDGSGYP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCCCCCCC
EGLKGDAIPVSARLMAVADVYDALISKRVYKPAFSHEQACTIILKGRGTHFDPDMVDAFV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
ELADDFRGIAQRYPDPH
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDQQRYVNAAPDRLVDKPIATVLVVDDTPDNLTLMAGLLQGHYRVKVAASGEKALVIAQS
CCCCCCCCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEEEECCCEEEEEECC
DAPPDLLLLDIMMPGIDGYEVCRRLKANEATRHIPVIFLTARSDPDDEREGLALGAVDYI
CCCCCEEEEEHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHCCEEEEHHHHH
TKPISPSILLARVATHLALKATADFLRDKSAYLEREVAMRTLEVQAIQDVTIMAMTSLAE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRDNETGNHIRRTQLYVKALAERLRHHPRFEAALNDRMIDLLYKSAPLHDIGKIGIPDSI
HCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCE
LLKPGKLTVEEFEIMKTHTTLGRKAIEDAEQRLGMRVAFLSVSKEIAYSHQEKWDGSGYP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCCCCCCC
EGLKGDAIPVSARLMAVADVYDALISKRVYKPAFSHEQACTIILKGRGTHFDPDMVDAFV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHH
ELADDFRGIAQRYPDPH
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]