Click here to switch to the map view.

The map label for this gene is pnp [H]

Identifier: 222110082

GI number: 222110082

Start: 891850

End: 894033

Strand: Direct

Name: pnp [H]

Synonym: Dtpsy_0868

Alternate gene names: 222110082

Gene position: 891850-894033 (Clockwise)

Preceding gene: 222110081

Following gene: 222110083

Centisome position: 23.49

GC content: 64.65

Gene sequence:

>2184_bases
ATGAGCATTTTTAACAAGGTTACCAAGTCGTTCCAGTGGGGTGACAAGACCGTTGTCATGGAAACGGGTGAGATCGCCCG
GCAGGCCTCAGGCGCCGTGCTGGTGAACATTGACGACACCGTGGTGCTCGCTACCGTGGTGGGGTCCAAGCAGGCCAAGC
CTGGCCAGGACTTCTTCCCGCTGACGGTGGACTACATCGAGAAGACCTACGCCGCCGGCAAGATCCCCGGCAGCTTCTTC
AAGCGCGAGGCCAAGCCCAGCGAGTTGGAGACGCTGACCAGCCGCCTGATCGATCGCCCCATCCGCCCGCTGTTCCCCGA
GGGCTTCTACAACGAGGTGCATGTGGTCATCCACACCATCTCGCTGAACCCCGAAGTGGACGCTGACATCGCCGCCATGA
TCGCCGTGAGCGCAGCGCTGTCCGTTTCCGGTATTCCGTTCAATGGCCCCATCGGCGCCGCACGCGTGGGCTACGTCAAT
GGCGAATACGTGCTGAACCCCGGGCAGACCGCGCGCAAGAGCTCGCAGCTGGACCTGGTGGTGGCGGGCACCGAGGCCGC
TGTGCTGATGGTGGAGTCCGAAGCGCAGCAGTTGTCCGAAGAGATCATGCTGGGCGCCGTGGTGTTCGGCCACGAGCAGG
GCAAGGTGGCGATCAACGCCATCCATGAGCTGGTGCGCGACGCTGGCAAGCCCGTGTGGGACTGGCAGCCGCCTGCCAAG
GACGAGACTTTCATCGCCAAGGTCACGGCCCTGGCAGAGGACAAGCTGCGCGCGGCTTACCAGATCCGCAGCAAGCAGGC
CCGCACCCAGGCCTTGCGCGAGGCATCGGCCTCGGTGCTGGAATCGCTGAAGGGCGAGGGCGTGGAGTTCGACGCCGTGA
AGGTCGAGGCCCTGCTGTTCGACATCGAAGCCAAGATCGTGCGCAGCCAGATCCTGGCCGGCGAGCCCCGCATCGACGGC
CGCGACACGCGCACCGTGCGCCCCATCGAGATCCGCAACTCCGTGCTGCCCCGCACCCACGGCTCCGCCCTGTTCACGCG
TGGCGAGACCCAGGCGCTGGTGGTGTCCACGCTGGGCACCGAGCGCGATGCGCAGCGCATCGACGCACTGGCCGGCGAGT
TCGAAGACCGTTTCATCTTCCACTACAACATGCCTCCCTTCGCCACGGGTGAAGTGGGCCGCATGGGCTCGACCAAGCGC
CGCGAAATCGGCCACGGCCGTCTGGCCAAGCGCGCCCTGGTGGCCTGCCTGCCGAGCAAGGACGAGTTCCCCTACACCAT
CCGCGTGGTGTCGGAGATCACCGAATCCAACGGCTCCTCGTCCATGGCATCGGTCTGCGGCGGCTGCCTGTCGCTGATGG
ATGCCGGCGTGCCCATGAAGGCGCACGTGGCCGGTATCGCCATGGGCTTGATCAAGGAAGACAACCGCTTCGCCGTGCTG
ACCGACATCCTGGGCGATGAGGACCACCTGGGCGACATGGACTTCAAGGTGGCCGGTACGACCAACGGCATCACCGCCCT
GCAGATGGACATCAAGATCCAGGGCATCACCAAGGAAATCATGCAGGTCGCCCTGGCGCAGGCCAAGGAAGCACGTATGC
ACATCCTGGGCAAGATGCAGGAAGCCATGGGTGAAGCCAAGACCGAGATCTCATCCTTCGCGCCCAAGCTCTACACGATG
AAGATCAACCCCGAGAAGATCCGCGACGTGATCGGCAAGGGCGGCGCCACGATCCGTGCCCTGACGGACGAGACCGGCTG
CCAGATCAACATCGAGGAAGACGGCACCATCACCATCGCAGCCACCGAGGCTGCCAAGGCTGATGAAGCCAAGCGCCGCA
TCGAAGAGATCACGGCCGAGGTTGAAGTGGGCAAGGTCTACGAAGGCCCGGTCACCAAGATCCTGGACTTCGGCGCGTTG
ATCAACCTGCTGCCCGGCAAGGACGGCCTGCTGCACATCAGCCAGATCGCCCACGAGCGTGTGGAAAAGGTGGGCGACTA
CCTGCAGGAAGGTCAGGTCGTGAAGGTCAAGGTGCTGGAGACCGACGACAAGGGCCGCGTCAAGCTGTCCATGAAGGCGC
TGGCTGACCGCCCCGCTGGCGACAGCGGCCGTCCCGCCCCTGCGGAGCGGGGCGAGCGTCGTGAGCGCCGTGATGGCGGT
GCTTCCGAGCAACAGCAGCAGTAA

Upstream 100 bases:

>100_bases
GGCAGGGCAGGGTCGCTGGAATGGCATCGTGTTCTGAATTGCGCTCCAAGCCTTGCTGGCACTGTGCTGCCAGCTATTAT
TTTGAGAGCAAGGAGTCACT

Downstream 100 bases:

>100_bases
GCCGGGGGTGTTCCTTCGGGAATACCTGTTTGCTATTGAAATGAGAGCTGCTAACGCCCGATGGACGGGCGCTAGCGGCT
CAAATGATTCAAAACTGCCA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 727; Mature: 726

Protein sequence:

>727_residues
MSIFNKVTKSFQWGDKTVVMETGEIARQASGAVLVNIDDTVVLATVVGSKQAKPGQDFFPLTVDYIEKTYAAGKIPGSFF
KREAKPSELETLTSRLIDRPIRPLFPEGFYNEVHVVIHTISLNPEVDADIAAMIAVSAALSVSGIPFNGPIGAARVGYVN
GEYVLNPGQTARKSSQLDLVVAGTEAAVLMVESEAQQLSEEIMLGAVVFGHEQGKVAINAIHELVRDAGKPVWDWQPPAK
DETFIAKVTALAEDKLRAAYQIRSKQARTQALREASASVLESLKGEGVEFDAVKVEALLFDIEAKIVRSQILAGEPRIDG
RDTRTVRPIEIRNSVLPRTHGSALFTRGETQALVVSTLGTERDAQRIDALAGEFEDRFIFHYNMPPFATGEVGRMGSTKR
REIGHGRLAKRALVACLPSKDEFPYTIRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKEDNRFAVL
TDILGDEDHLGDMDFKVAGTTNGITALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQEAMGEAKTEISSFAPKLYTM
KINPEKIRDVIGKGGATIRALTDETGCQINIEEDGTITIAATEAAKADEAKRRIEEITAEVEVGKVYEGPVTKILDFGAL
INLLPGKDGLLHISQIAHERVEKVGDYLQEGQVVKVKVLETDDKGRVKLSMKALADRPAGDSGRPAPAERGERRERRDGG
ASEQQQQ

Sequences:

>Translated_727_residues
MSIFNKVTKSFQWGDKTVVMETGEIARQASGAVLVNIDDTVVLATVVGSKQAKPGQDFFPLTVDYIEKTYAAGKIPGSFF
KREAKPSELETLTSRLIDRPIRPLFPEGFYNEVHVVIHTISLNPEVDADIAAMIAVSAALSVSGIPFNGPIGAARVGYVN
GEYVLNPGQTARKSSQLDLVVAGTEAAVLMVESEAQQLSEEIMLGAVVFGHEQGKVAINAIHELVRDAGKPVWDWQPPAK
DETFIAKVTALAEDKLRAAYQIRSKQARTQALREASASVLESLKGEGVEFDAVKVEALLFDIEAKIVRSQILAGEPRIDG
RDTRTVRPIEIRNSVLPRTHGSALFTRGETQALVVSTLGTERDAQRIDALAGEFEDRFIFHYNMPPFATGEVGRMGSTKR
REIGHGRLAKRALVACLPSKDEFPYTIRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKEDNRFAVL
TDILGDEDHLGDMDFKVAGTTNGITALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQEAMGEAKTEISSFAPKLYTM
KINPEKIRDVIGKGGATIRALTDETGCQINIEEDGTITIAATEAAKADEAKRRIEEITAEVEVGKVYEGPVTKILDFGAL
INLLPGKDGLLHISQIAHERVEKVGDYLQEGQVVKVKVLETDDKGRVKLSMKALADRPAGDSGRPAPAERGERRERRDGG
ASEQQQQ
>Mature_726_residues
SIFNKVTKSFQWGDKTVVMETGEIARQASGAVLVNIDDTVVLATVVGSKQAKPGQDFFPLTVDYIEKTYAAGKIPGSFFK
REAKPSELETLTSRLIDRPIRPLFPEGFYNEVHVVIHTISLNPEVDADIAAMIAVSAALSVSGIPFNGPIGAARVGYVNG
EYVLNPGQTARKSSQLDLVVAGTEAAVLMVESEAQQLSEEIMLGAVVFGHEQGKVAINAIHELVRDAGKPVWDWQPPAKD
ETFIAKVTALAEDKLRAAYQIRSKQARTQALREASASVLESLKGEGVEFDAVKVEALLFDIEAKIVRSQILAGEPRIDGR
DTRTVRPIEIRNSVLPRTHGSALFTRGETQALVVSTLGTERDAQRIDALAGEFEDRFIFHYNMPPFATGEVGRMGSTKRR
EIGHGRLAKRALVACLPSKDEFPYTIRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKEDNRFAVLT
DILGDEDHLGDMDFKVAGTTNGITALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQEAMGEAKTEISSFAPKLYTMK
INPEKIRDVIGKGGATIRALTDETGCQINIEEDGTITIAATEAAKADEAKRRIEEITAEVEVGKVYEGPVTKILDFGALI
NLLPGKDGLLHISQIAHERVEKVGDYLQEGQVVKVKVLETDDKGRVKLSMKALADRPAGDSGRPAPAERGERRERRDGGA
SEQQQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=705, Percent_Identity=37.3049645390071, Blast_Score=427, Evalue=1e-119,
Organism=Escherichia coli, GI145693187, Length=690, Percent_Identity=65.5072463768116, Blast_Score=932, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=711, Percent_Identity=31.7862165963432, Blast_Score=318, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI17535281, Length=87, Percent_Identity=44.8275862068966, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281362905, Length=723, Percent_Identity=36.237897648686, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=723, Percent_Identity=36.237897648686, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=723, Percent_Identity=36.237897648686, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=670, Percent_Identity=36.1194029850746, Blast_Score=398, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78809; Mature: 78678

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFNKVTKSFQWGDKTVVMETGEIARQASGAVLVNIDDTVVLATVVGSKQAKPGQDFFP
CCHHHHHHHHHCCCCEEEEEECHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCCCC
LTVDYIEKTYAAGKIPGSFFKREAKPSELETLTSRLIDRPIRPLFPEGFYNEVHVVIHTI
CHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
SLNPEVDADIAAMIAVSAALSVSGIPFNGPIGAARVGYVNGEYVLNPGQTARKSSQLDLV
ECCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCCHHCCCCCEEEE
VAGTEAAVLMVESEAQQLSEEIMLGAVVFGHEQGKVAINAIHELVRDAGKPVWDWQPPAK
EECCCEEEEEEEHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
DETFIAKVTALAEDKLRAAYQIRSKQARTQALREASASVLESLKGEGVEFDAVKVEALLF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHEEEEEHHH
DIEAKIVRSQILAGEPRIDGRDTRTVRPIEIRNSVLPRTHGSALFTRGETQALVVSTLGT
HHHHHHHHHHHHCCCCCCCCCCCCEECEEHHHCCCCCCCCCCEEEECCCCCEEEEEECCC
ERDAQRIDALAGEFEDRFIFHYNMPPFATGEVGRMGSTKRREIGHGRLAKRALVACLPSK
CHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHCCCCC
DEFPYTIRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKEDNRFAVL
CCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCEEEE
TDILGDEDHLGDMDFKVAGTTNGITALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQ
EECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
EAMGEAKTEISSFAPKLYTMKINPEKIRDVIGKGGATIRALTDETGCQINIEEDGTITIA
HHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEE
ATEAAKADEAKRRIEEITAEVEVGKVYEGPVTKILDFGALINLLPGKDGLLHISQIAHER
ECCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VEKVGDYLQEGQVVKVKVLETDDKGRVKLSMKALADRPAGDSGRPAPAERGERRERRDGG
HHHHHHHHHCCCEEEEEEEECCCCCCEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHCCC
ASEQQQQ
CCCCCCC
>Mature Secondary Structure 
SIFNKVTKSFQWGDKTVVMETGEIARQASGAVLVNIDDTVVLATVVGSKQAKPGQDFFP
CHHHHHHHHHCCCCEEEEEECHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCCCC
LTVDYIEKTYAAGKIPGSFFKREAKPSELETLTSRLIDRPIRPLFPEGFYNEVHVVIHTI
CHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
SLNPEVDADIAAMIAVSAALSVSGIPFNGPIGAARVGYVNGEYVLNPGQTARKSSQLDLV
ECCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCCHHCCCCCEEEE
VAGTEAAVLMVESEAQQLSEEIMLGAVVFGHEQGKVAINAIHELVRDAGKPVWDWQPPAK
EECCCEEEEEEEHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
DETFIAKVTALAEDKLRAAYQIRSKQARTQALREASASVLESLKGEGVEFDAVKVEALLF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHEEEEEHHH
DIEAKIVRSQILAGEPRIDGRDTRTVRPIEIRNSVLPRTHGSALFTRGETQALVVSTLGT
HHHHHHHHHHHHCCCCCCCCCCCCEECEEHHHCCCCCCCCCCEEEECCCCCEEEEEECCC
ERDAQRIDALAGEFEDRFIFHYNMPPFATGEVGRMGSTKRREIGHGRLAKRALVACLPSK
CHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHCCCCC
DEFPYTIRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKEDNRFAVL
CCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCEEEE
TDILGDEDHLGDMDFKVAGTTNGITALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQ
EECCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
EAMGEAKTEISSFAPKLYTMKINPEKIRDVIGKGGATIRALTDETGCQINIEEDGTITIA
HHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEE
ATEAAKADEAKRRIEEITAEVEVGKVYEGPVTKILDFGALINLLPGKDGLLHISQIAHER
ECCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VEKVGDYLQEGQVVKVKVLETDDKGRVKLSMKALADRPAGDSGRPAPAERGERRERRDGG
HHHHHHHHHCCCEEEEEEEECCCCCCEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHCCC
ASEQQQQ
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA