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The map label for this gene is yaaI [H]

Identifier: 222110067

GI number: 222110067

Start: 879440

End: 880024

Strand: Direct

Name: yaaI [H]

Synonym: Dtpsy_0853

Alternate gene names: 222110067

Gene position: 879440-880024 (Clockwise)

Preceding gene: 222110066

Following gene: 222110068

Centisome position: 23.16

GC content: 66.67

Gene sequence:

>585_bases
GTGGCGCGCCCCGCTGCATTGCGCCGCACGCCGCTGCCGCGCAGCAAACACGTGCTGCTGTTGGTGGATGTGATCAATCC
GCTGAATTTCCCTACAGCGGCAGACCTGCTGCCCGGAGCGGTACGCGCCGCGAACGCCATTGCGCGGCTGAAGGCACGCC
TGGCGCGGCGCGGGGTCACGGCGATCTATGCCAACGACAACTACGGCACGTGGCACAGCGACTTCCGTGACATTCTCACG
GCCTGCCGCGCGCTGCCGGGGGCGCGCGGCGAGATGGCCTGCCTGCTTGCGCCCGCGCCGCAGGATCTGGTGATCCTCAA
GCCACAGCATTCGGCGTTCCATTCGACTCCCTTGCAGCATTTGCTGGACAAGATGCAGACCGAGCAGCTGATCATCGTGG
GCCTGGCCGCCGACATGTGCGTGATGCTGACGGCCACCGATGCGCGCATGCTGGGCTACAAGGTGTGGGTGCCGTCGGAT
TGCACCGCCGCGGAATCGGAACACCGCAAGACGGAGGCCCTGCGGCAATTGCATGAGGTGTTCAAGTGCGCCGTACGGCC
TGCGGTACGGGCGGCTGCACCCTGA

Upstream 100 bases:

>100_bases
GGCGGTGCTGCCTCCGCCGGAAGAGGTCAAACAGGTAGAGACGGCCATTGGCGCGTCGCGCGCGGAGCAGCAACGCGACG
CGATGCTTTGAGGAGGCTGC

Downstream 100 bases:

>100_bases
CTGACCGGAGCGACCGCTATGTTCAACCTGGACTTACCCGCCGCAGAGCTGGTGCTGCGCGCCGCCATGGTCTACGGCCT
GCTGCTGGCGCTCATGCGCG

Product: isochorismatase hydrolase

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MARPAALRRTPLPRSKHVLLLVDVINPLNFPTAADLLPGAVRAANAIARLKARLARRGVTAIYANDNYGTWHSDFRDILT
ACRALPGARGEMACLLAPAPQDLVILKPQHSAFHSTPLQHLLDKMQTEQLIIVGLAADMCVMLTATDARMLGYKVWVPSD
CTAAESEHRKTEALRQLHEVFKCAVRPAVRAAAP

Sequences:

>Translated_194_residues
MARPAALRRTPLPRSKHVLLLVDVINPLNFPTAADLLPGAVRAANAIARLKARLARRGVTAIYANDNYGTWHSDFRDILT
ACRALPGARGEMACLLAPAPQDLVILKPQHSAFHSTPLQHLLDKMQTEQLIIVGLAADMCVMLTATDARMLGYKVWVPSD
CTAAESEHRKTEALRQLHEVFKCAVRPAVRAAAP
>Mature_193_residues
ARPAALRRTPLPRSKHVLLLVDVINPLNFPTAADLLPGAVRAANAIARLKARLARRGVTAIYANDNYGTWHSDFRDILTA
CRALPGARGEMACLLAPAPQDLVILKPQHSAFHSTPLQHLLDKMQTEQLIIVGLAADMCVMLTATDARMLGYKVWVPSDC
TAAESEHRKTEALRQLHEVFKCAVRPAVRAAAP

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 21198; Mature: 21067

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPAALRRTPLPRSKHVLLLVDVINPLNFPTAADLLPGAVRAANAIARLKARLARRGVT
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AIYANDNYGTWHSDFRDILTACRALPGARGEMACLLAPAPQDLVILKPQHSAFHSTPLQH
EEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEECCCCCCHHCCHHHH
LLDKMQTEQLIIVGLAADMCVMLTATDARMLGYKVWVPSDCTAAESEHRKTEALRQLHEV
HHHHHCCCCEEEEEEHHHHEEEEEECCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHH
FKCAVRPAVRAAAP
HHHHHHHHHHCCCC
>Mature Secondary Structure 
ARPAALRRTPLPRSKHVLLLVDVINPLNFPTAADLLPGAVRAANAIARLKARLARRGVT
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AIYANDNYGTWHSDFRDILTACRALPGARGEMACLLAPAPQDLVILKPQHSAFHSTPLQH
EEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEECCCCCCHHCCHHHH
LLDKMQTEQLIIVGLAADMCVMLTATDARMLGYKVWVPSDCTAAESEHRKTEALRQLHEV
HHHHHCCCCEEEEEEHHHHEEEEEECCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHH
FKCAVRPAVRAAAP
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]