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The map label for this gene is opuE [H]

Identifier: 222110015

GI number: 222110015

Start: 832526

End: 833977

Strand: Direct

Name: opuE [H]

Synonym: Dtpsy_0800

Alternate gene names: 222110015

Gene position: 832526-833977 (Clockwise)

Preceding gene: 222110014

Following gene: 222110016

Centisome position: 21.93

GC content: 63.09

Gene sequence:

>1452_bases
GTGTTGCTCTTCATGGTGATTGCCTACCTGTTTGTCACCATCGGCATTGGCTTGTGGGCTGCCCAACGGGTGAAGAACAC
GGCTGATTTCGCGATTGCCGGACGCCACCTGCCCATGTACATGATCATCACCACCACATTCGCCACGTGGTTCGGCTCCG
AGATCGTGCTGGGCGTGCCCGCCAAGTTCATTGAAGGGGGGCTCAAGGCCGTGGTGGAAGACCCGTTTGGCGCCGGCATG
TGCTTGATTCTGGTGGGATTGTTCTTCGCGGCCAAGCTCTACCGCATGACGCTGCTGACCATCAGCGACTACTACCGCGA
GCGCTACGGCCGCGCAGTGGAGGTGATCTGCTCGCTCATCATCATGCTCAGCTACCTGGGCTGGGTGTCGGCGCAGGTCA
CGGCGCTGGGGCTGGTGTTCAATCTGCTGTCCGGCGGTGCGGTGAGCATCCCCATGGGCATGGCCATCGGCGTGCTGTCC
ATCCTGGTCTACACGCTGTGGGGTGGCATGTGGTCGGTGGCGGTGACCGATTTCATCCAGATGATCATTCTGGTGGTCGG
CCTGGTGGTGCTGGCCTGGTTCGCCAGCGACATGGCGGGGGGCGCGGGCAAGGTGATCGAGCTGGCCACCAGCCGCGACC
TGTTTCAGTTCTGGCCCGAACCCACCTGGCACGAAATCATCTTCTTCTTCGGTGCCGCCATCACCATGATGCTGGGCTCC
ATCCCGCAGCAGGATGTGTTCCAGCGCGTCATGTCGGCCAATACCGAACGCGCCGCCACGCATGGCACGGTGATCGGCGG
TACGGCCTACATCTTCTTCGCCTTCGTGCCCATGTTCCTCGTGGCAAGCGCGCTGCTGATCATGCCGCAGGAGACGGCTG
AACTGCTCAAGGATGACCCGCAGAAGGTGCTGCCCACTCTGGTGATGGACAAGATGCCGCTCGTCATGCAGGTGCTGTTC
TTCGGTGCGCTACTGTCGGCCATCAAGTCCTGCGCCTCGGCCACACTGCTGGCGCCCAGCGTCACCTTCACCGAGAACAT
CTGGCGGCAGTTCCGCCCTGAGAAGATCAGCGACCGCGAGAACCTGCTGGCCATGCGCATCAGTGTGCTGGTGTTCGCGG
TGGGTGTGCTGGCCTATTCCATCAAGATGGAAGGCACGCCCATTTACGAGCTGGTGTCCAGCGCCTACCAAGTGCCGCTG
GTGGGGGCGTTTGTGCCGCTGGTATTCGGCCTGTACTGGAAGCGTGCCAACACGCAAGGCGCGCTGTGCGCGGTGGCGCT
GGGCATTGGCGTGTGGCTGGTGTTCATGGTCACCCCCGCGCTGCATGAGGCGTTCCCCCAGCAACTCGCCGGCTTGCTGG
CCGCCATGGTCGGCATGGTGGCCGGTTCGCTTGCGCCGCAGGTGGTGCGCAATTCCCATGCGCCGCACCACCGGGTGGTG
GGGGTGGAGTGA

Upstream 100 bases:

>100_bases
GCGGCCTGGGCTTCGTGCTGGGGCTGCTGGTCATGCAGCTCTTCGTGCGCGTGCGCATCTTCTAACTTCCTTCCCTCTCT
CATTCCTCAGGAGTACCAGC

Downstream 100 bases:

>100_bases
CGCACGGGCGCGCGGTGCGGCCTGGGGTGCATGCCTATAATTGAAGGTTTTGCACATTCTTCGCACTTCTTCGTAATGCC
TATTTACGCCTATCGATGCG

Product: Na+/solute symporter

Products: Na (I) [Cytoplasm]; pantothenate [Cytoplasm] [C]

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MLLFMVIAYLFVTIGIGLWAAQRVKNTADFAIAGRHLPMYMIITTTFATWFGSEIVLGVPAKFIEGGLKAVVEDPFGAGM
CLILVGLFFAAKLYRMTLLTISDYYRERYGRAVEVICSLIIMLSYLGWVSAQVTALGLVFNLLSGGAVSIPMGMAIGVLS
ILVYTLWGGMWSVAVTDFIQMIILVVGLVVLAWFASDMAGGAGKVIELATSRDLFQFWPEPTWHEIIFFFGAAITMMLGS
IPQQDVFQRVMSANTERAATHGTVIGGTAYIFFAFVPMFLVASALLIMPQETAELLKDDPQKVLPTLVMDKMPLVMQVLF
FGALLSAIKSCASATLLAPSVTFTENIWRQFRPEKISDRENLLAMRISVLVFAVGVLAYSIKMEGTPIYELVSSAYQVPL
VGAFVPLVFGLYWKRANTQGALCAVALGIGVWLVFMVTPALHEAFPQQLAGLLAAMVGMVAGSLAPQVVRNSHAPHHRVV
GVE

Sequences:

>Translated_483_residues
MLLFMVIAYLFVTIGIGLWAAQRVKNTADFAIAGRHLPMYMIITTTFATWFGSEIVLGVPAKFIEGGLKAVVEDPFGAGM
CLILVGLFFAAKLYRMTLLTISDYYRERYGRAVEVICSLIIMLSYLGWVSAQVTALGLVFNLLSGGAVSIPMGMAIGVLS
ILVYTLWGGMWSVAVTDFIQMIILVVGLVVLAWFASDMAGGAGKVIELATSRDLFQFWPEPTWHEIIFFFGAAITMMLGS
IPQQDVFQRVMSANTERAATHGTVIGGTAYIFFAFVPMFLVASALLIMPQETAELLKDDPQKVLPTLVMDKMPLVMQVLF
FGALLSAIKSCASATLLAPSVTFTENIWRQFRPEKISDRENLLAMRISVLVFAVGVLAYSIKMEGTPIYELVSSAYQVPL
VGAFVPLVFGLYWKRANTQGALCAVALGIGVWLVFMVTPALHEAFPQQLAGLLAAMVGMVAGSLAPQVVRNSHAPHHRVV
GVE
>Mature_483_residues
MLLFMVIAYLFVTIGIGLWAAQRVKNTADFAIAGRHLPMYMIITTTFATWFGSEIVLGVPAKFIEGGLKAVVEDPFGAGM
CLILVGLFFAAKLYRMTLLTISDYYRERYGRAVEVICSLIIMLSYLGWVSAQVTALGLVFNLLSGGAVSIPMGMAIGVLS
ILVYTLWGGMWSVAVTDFIQMIILVVGLVVLAWFASDMAGGAGKVIELATSRDLFQFWPEPTWHEIIFFFGAAITMMLGS
IPQQDVFQRVMSANTERAATHGTVIGGTAYIFFAFVPMFLVASALLIMPQETAELLKDDPQKVLPTLVMDKMPLVMQVLF
FGALLSAIKSCASATLLAPSVTFTENIWRQFRPEKISDRENLLAMRISVLVFAVGVLAYSIKMEGTPIYELVSSAYQVPL
VGAFVPLVFGLYWKRANTQGALCAVALGIGVWLVFMVTPALHEAFPQQLAGLLAAMVGMVAGSLAPQVVRNSHAPHHRVV
GVE

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI11141885, Length=454, Percent_Identity=26.6519823788546, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI109659836, Length=495, Percent_Identity=25.6565656565657, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI14140236, Length=494, Percent_Identity=24.4939271255061, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI109659839, Length=511, Percent_Identity=24.853228962818, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI4507031, Length=466, Percent_Identity=22.3175965665236, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI4507033, Length=481, Percent_Identity=23.4927234927235, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI4507035, Length=446, Percent_Identity=25.5605381165919, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI17941285, Length=459, Percent_Identity=22.8758169934641, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI206597483, Length=479, Percent_Identity=22.1294363256785, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI206597487, Length=411, Percent_Identity=22.3844282238443, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI256985183, Length=444, Percent_Identity=23.4234234234234, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI87082237, Length=482, Percent_Identity=26.1410788381743, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787251, Length=445, Percent_Identity=24.9438202247191, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790503, Length=451, Percent_Identity=21.0643015521064, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539284, Length=439, Percent_Identity=26.879271070615, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24648033, Length=444, Percent_Identity=26.8018018018018, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356865, Length=444, Percent_Identity=26.8018018018018, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640370, Length=420, Percent_Identity=23.5714285714286, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 52580; Mature: 52580

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCC
>Mature Secondary Structure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CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; pantothenate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + pantothenate [Periplasm] = Na (I) [Cytoplasm] + pantothenate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]