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The map label for this gene is proS

Identifier: 222109990

GI number: 222109990

Start: 802848

End: 804593

Strand: Direct

Name: proS

Synonym: Dtpsy_0775

Alternate gene names: 222109990

Gene position: 802848-804593 (Clockwise)

Preceding gene: 222109988

Following gene: 222109991

Centisome position: 21.15

GC content: 64.95

Gene sequence:

>1746_bases
ATGAAAGCTTCCCAATTTTTCATTTCCACCTTGAAAGAGGCGCCTGCCGACGCCGAGGTTGTCAGTCACCAACTCATGAT
GCGCGCAGGCCTCATCAAGAAGTTGGGTGCCGGGATTTACAACTACATGCCCATGGGCCTGCGCGTGATCCGCAAGGTGG
AAGCCATCGTGCGCGAGGAGATGAACCGCGCAGGTGCCGTGGAATGCACCATGCCCGTGGTGCAGCCCGCCGAGCTGTGG
CAGGAGACTGGGCGCTTCGACAAGATGGGCCCCGAGTTGCTGCGTATCCACGACCGCCATGGGCGTGACTTCGTGATCCA
GCCCACCAGCGAGGAAGTGGTCACTGACATCGCACGCCAGGAACTGCGCAGCTACAAGCAGCTGCCCAAGAACCTCTACC
AGATCCAGACCAAGTTCCGCGATGAACGCCGCCCGCGCTTCGGACTGATGCGCGGGCGCGAATTCATCATGAAGGACGCC
TACAGCTTCGACCGTGACCAGGCCGGCGCCAAGGCGAGCTACCAGGTGATGGCGCAGGCCTATCGCCGCATCTTCGACCG
CTTTGGCCTGCGCTACCGCGCCGTGGCGGCGGATTCAGGCGCCATCGGTGGCGACTTGAGCGAAGAGTTCCAGGTGATCG
CCGCCACGGGTGAGGACGCGATCGTCTACTGCCCCGCCAGCGATTACGCGGCCAACATGGAAAAGGCCGAAGCGCTGGCC
CCCGCGGGCGCGCGTCCCGCGGCCAAGCAGCCGCTGACGGTGACACCCACGCCTGGCAAGAGCACCTGCGCGGACGTGGC
CGAACTGCTGAGCGTGCCGCTGAGCACTACGGTGAAATCGCTGGTGCTGGCCACGGACGAAACCGACGCGGCGGGCGAGA
TCATCCGCAGCCAGGTCTGGCTGCTGCTGCTGCGCGGTGACCACGACATGAACGAGATCAAGGTGGCCAAGGTGCCGGGC
CTGGACGCGGGTTTCCGCTTTGCCACCGTGGCCGAGATCGCGGACCACTTTGGCTGCAAGCCGGGCTACCTGGGCCCGCT
GAACCTACAGAAGCCAGTGAAGCTGGTGGTGGACCGCGACGTGGCCGTGATGGCCGACTGGATCTGCGGAGCCAACCAGG
AAGGTCACCACATCACCGGCGTGAACTGGGGCCGCGACCTGCCGGAGCCCGACATGGTGGCCGACCTGCGCAATGTGGTG
GCTGGCGACCTGTCGCCGGACGGCCAGGGGGAACTTGCCATCGAGCGCGGCATCGAAGTCGGCCATGTGTTCTACCTCGG
CACCAAGTATTCCAAAGCCATGAATGCCACCTTCCTCGGCGAAGACGGCAAGCCCGCCTTCTTCGAGATGGGCTGCTACG
GCATTGGCGTCACGCGCCTGCCGGCAGCGGCCATTGAGCAGAACCATGACGAGCGCGGCATCATCTGGCCCGACGCCATC
GCCCCGTTCACCGTGGTCATCTGCCCCGTGGGCATGGACCGCAGCGAAGCCGTCAAGGCACAGGCCGAGTCGCTGTACGC
CGACCTGCTAGCCGCCGGTGTGGACGTGATCCTGGACGACCGCGGCGAGCGCCCGGGCGCCATGTTTGCCGATTGGGAAC
TGATCGGCGTGCCCCATCGCGTGACCATTGGCGACAAGAGCCTCAAGGAAGGGCAGGTCGAATACCAGCACCGCCGCGAT
GCATCTGCCACCAAAGTGGGCGTGGCGGACATCCTGGCGCATGTGAAGGAACGGCTGGCAGCATGA

Upstream 100 bases:

>100_bases
GGTCGAGCATAATCTAACCCCAAATTTTTAAAACTGCGGTCATTATGCACGCTACCTCGCGTGCGGCAAGCGCACCCGCC
CCAGTTGTCAAGCCCGTTCG

Downstream 100 bases:

>100_bases
ACGCGGCGGGAGGTGGTCCAATGACCGACGAACGGCAGGGCCGGCTGTCGCGCCGCACCTGCCTGGCTTCGCTGGCGGCG
GGGCCGGCTGCATGGCTGGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MKASQFFISTLKEAPADAEVVSHQLMMRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREEMNRAGAVECTMPVVQPAELW
QETGRFDKMGPELLRIHDRHGRDFVIQPTSEEVVTDIARQELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDA
YSFDRDQAGAKASYQVMAQAYRRIFDRFGLRYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPASDYAANMEKAEALA
PAGARPAAKQPLTVTPTPGKSTCADVAELLSVPLSTTVKSLVLATDETDAAGEIIRSQVWLLLLRGDHDMNEIKVAKVPG
LDAGFRFATVAEIADHFGCKPGYLGPLNLQKPVKLVVDRDVAVMADWICGANQEGHHITGVNWGRDLPEPDMVADLRNVV
AGDLSPDGQGELAIERGIEVGHVFYLGTKYSKAMNATFLGEDGKPAFFEMGCYGIGVTRLPAAAIEQNHDERGIIWPDAI
APFTVVICPVGMDRSEAVKAQAESLYADLLAAGVDVILDDRGERPGAMFADWELIGVPHRVTIGDKSLKEGQVEYQHRRD
ASATKVGVADILAHVKERLAA

Sequences:

>Translated_581_residues
MKASQFFISTLKEAPADAEVVSHQLMMRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREEMNRAGAVECTMPVVQPAELW
QETGRFDKMGPELLRIHDRHGRDFVIQPTSEEVVTDIARQELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDA
YSFDRDQAGAKASYQVMAQAYRRIFDRFGLRYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPASDYAANMEKAEALA
PAGARPAAKQPLTVTPTPGKSTCADVAELLSVPLSTTVKSLVLATDETDAAGEIIRSQVWLLLLRGDHDMNEIKVAKVPG
LDAGFRFATVAEIADHFGCKPGYLGPLNLQKPVKLVVDRDVAVMADWICGANQEGHHITGVNWGRDLPEPDMVADLRNVV
AGDLSPDGQGELAIERGIEVGHVFYLGTKYSKAMNATFLGEDGKPAFFEMGCYGIGVTRLPAAAIEQNHDERGIIWPDAI
APFTVVICPVGMDRSEAVKAQAESLYADLLAAGVDVILDDRGERPGAMFADWELIGVPHRVTIGDKSLKEGQVEYQHRRD
ASATKVGVADILAHVKERLAA
>Mature_581_residues
MKASQFFISTLKEAPADAEVVSHQLMMRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREEMNRAGAVECTMPVVQPAELW
QETGRFDKMGPELLRIHDRHGRDFVIQPTSEEVVTDIARQELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDA
YSFDRDQAGAKASYQVMAQAYRRIFDRFGLRYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPASDYAANMEKAEALA
PAGARPAAKQPLTVTPTPGKSTCADVAELLSVPLSTTVKSLVLATDETDAAGEIIRSQVWLLLLRGDHDMNEIKVAKVPG
LDAGFRFATVAEIADHFGCKPGYLGPLNLQKPVKLVVDRDVAVMADWICGANQEGHHITGVNWGRDLPEPDMVADLRNVV
AGDLSPDGQGELAIERGIEVGHVFYLGTKYSKAMNATFLGEDGKPAFFEMGCYGIGVTRLPAAAIEQNHDERGIIWPDAI
APFTVVICPVGMDRSEAVKAQAESLYADLLAAGVDVILDDRGERPGAMFADWELIGVPHRVTIGDKSLKEGQVEYQHRRD
ASATKVGVADILAHVKERLAA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=249, Percent_Identity=44.1767068273092, Blast_Score=220, Evalue=2e-57,
Organism=Escherichia coli, GI1786392, Length=579, Percent_Identity=55.2677029360967, Blast_Score=672, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=237, Percent_Identity=37.9746835443038, Blast_Score=173, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=90, Percent_Identity=35.5555555555556, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=205, Percent_Identity=37.0731707317073, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321808, Length=189, Percent_Identity=25.9259259259259, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24656200, Length=251, Percent_Identity=38.6454183266932, Blast_Score=179, Evalue=7e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ACIET (B9ME00)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002552254.1
- ProteinModelPortal:   B9ME00
- SMR:   B9ME00
- GeneID:   7383715
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_0775
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63700; Mature: 63700

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASQFFISTLKEAPADAEVVSHQLMMRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVECTMPVVQPAELWQETGRFDKMGPELLRIHDRHGRDFVIQPTSEEVVTDIARQ
HHCCCCEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
ELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDAYSFDRDQAGAKASYQVMAQA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHH
YRRIFDRFGLRYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPASDYAANMEKAEALA
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHC
PAGARPAAKQPLTVTPTPGKSTCADVAELLSVPLSTTVKSLVLATDETDAAGEIIRSQVW
CCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHEEECCCCCHHHHHHHHHHE
LLLLRGDHDMNEIKVAKVPGLDAGFRFATVAEIADHFGCKPGYLGPLNLQKPVKLVVDRD
EEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
VAVMADWICGANQEGHHITGVNWGRDLPEPDMVADLRNVVAGDLSPDGQGELAIERGIEV
HHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEHCCCCCE
GHVFYLGTKYSKAMNATFLGEDGKPAFFEMGCYGIGVTRLPAAAIEQNHDERGIIWPDAI
EEEEEECCHHHHHCCCEEECCCCCCCEEECCCEECCHHHCCHHHHHCCCCCCCCCCCCCC
APFTVVICPVGMDRSEAVKAQAESLYADLLAAGVDVILDDRGERPGAMFADWELIGVPHR
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECEEEECCCEE
VTIGDKSLKEGQVEYQHRRDASATKVGVADILAHVKERLAA
EEECCCCHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASQFFISTLKEAPADAEVVSHQLMMRAGLIKKLGAGIYNYMPMGLRVIRKVEAIVREE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAVECTMPVVQPAELWQETGRFDKMGPELLRIHDRHGRDFVIQPTSEEVVTDIARQ
HHCCCCEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
ELRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDAYSFDRDQAGAKASYQVMAQA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHH
YRRIFDRFGLRYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPASDYAANMEKAEALA
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHC
PAGARPAAKQPLTVTPTPGKSTCADVAELLSVPLSTTVKSLVLATDETDAAGEIIRSQVW
CCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHEEECCCCCHHHHHHHHHHE
LLLLRGDHDMNEIKVAKVPGLDAGFRFATVAEIADHFGCKPGYLGPLNLQKPVKLVVDRD
EEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
VAVMADWICGANQEGHHITGVNWGRDLPEPDMVADLRNVVAGDLSPDGQGELAIERGIEV
HHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEHCCCCCE
GHVFYLGTKYSKAMNATFLGEDGKPAFFEMGCYGIGVTRLPAAAIEQNHDERGIIWPDAI
EEEEEECCHHHHHCCCEEECCCCCCCEEECCCEECCHHHCCHHHHHCCCCCCCCCCCCCC
APFTVVICPVGMDRSEAVKAQAESLYADLLAAGVDVILDDRGERPGAMFADWELIGVPHR
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECEEEECCCEE
VTIGDKSLKEGQVEYQHRRDASATKVGVADILAHVKERLAA
EEECCCCHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA