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The map label for this gene is proB

Identifier: 222109988

GI number: 222109988

Start: 800879

End: 802021

Strand: Direct

Name: proB

Synonym: Dtpsy_0773

Alternate gene names: 222109988

Gene position: 800879-802021 (Clockwise)

Preceding gene: 222109987

Following gene: 222109990

Centisome position: 21.09

GC content: 68.24

Gene sequence:

>1143_bases
ATGGTGTCCAGTGTATTGCGCGACGCGCGTCGCATCGTGGTCAAGGTAGGGTCCAGTCTCGTCACGAATGAGGGACGGGG
GCTGGACGAGGTGGCCATTGGGGAGTGGTGCCGCCAACTGGCAGCGCTGGTGCGGGGGCAGGGGGGCGAGCCGCGCGAGG
TCATCATGGTCTCCAGCGGAGCGATCGCCGAAGGCATGAAGCGCCTGGGCTGGAGCAGCCGCCCCGGCGAGATCCACGAA
CTGCAGGCCGCTGCCGCCGTGGGACAGATGGGGCTGGCGCAGATGTACGAGACCAAGCTGCGCGAGCAATCCATGGGCAG
TGCGCAGGTGCTGCTGACCCATGCCGACTTGGCGGACCGCGAGCGTTACCTGAATGCCCGCTCCACCCTGCTGACGCTGC
TGCGCCTGGGCGTGGTGCCGGTGATCAATGAGAACGACACCGTAGTCACCGATGAGATCAAGTTTGGTGACAACGATACT
CTGGGGGCTCTCGTCGCCAACCTCGTCGAAGCCGACGCGCTGGTGATCCTGACCGACCAGAAGGGGCTGTACACCGCCGA
TCCGCGGCGCGACCCGCAGGCGCAGTTCGTCCACGAGGCACAGGCTGGCGATGCGGCGCTGGAGGCGATGGCCGGCGGTG
CGGGCTCCAGCATCGGCAAGGGCGGGATGATCACCAAGATCCTCGCGGCCAAACGTGCGGCGGGCTCGGGTGCCTCCACC
GTCATCGCATGGGGGCGGGAGCCGGACGTGCTGCTGCGGCTGGCCCGGGGCGAATCGATCGGCACGTTGCTCGTGGCGCA
GACGCAGAAGAACCAAGCGCGCAAGCAGTGGATGGTGGACCACCTTCAACTGCGCGGCTCCGTGACGGTGGATGCCGGCG
CGGCGGCCAAGCTGCGCGAGGACGGTAAGAGCCTGCTGCCGATCGGCATGGTGGCGGTGGAGGGCGACTTCGTGCGCGGC
GATGTGATCGCGGTGCGCGACGCCGGCGGCACAGAGATCGCCCGTGGTCTCGCCAACTATGCGAGCGCCGAGGCGCGCCT
GCTGTGCCGCAAGCCTTCCGCAGAGTTCGAGCGCCTGCTGGGGTACGCGGCCGAGCCGGAGATGGTTCACCGCGACAACA
TGGTGGTGCTCGGCGCGCGATGA

Upstream 100 bases:

>100_bases
TTCGCTGGTGAGCCACCGCGTGGGTGATGCGAGGATTGCTATTTAATTTATAGCTGTTGGCGCTTGCCTGACAAGCGCTA
GGGCCTAATTTCTTATAAAA

Downstream 100 bases:

>100_bases
CGCTTAAGAAGGGTGCGTCTTGCGGACGCGTGCCTTCTTACACACGCTAGGCGTCCAGTGGTTTCTTGGGGACGGGCAGG
GTAGGCAGCGCCTCCAACGG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MVSSVLRDARRIVVKVGSSLVTNEGRGLDEVAIGEWCRQLAALVRGQGGEPREVIMVSSGAIAEGMKRLGWSSRPGEIHE
LQAAAAVGQMGLAQMYETKLREQSMGSAQVLLTHADLADRERYLNARSTLLTLLRLGVVPVINENDTVVTDEIKFGDNDT
LGALVANLVEADALVILTDQKGLYTADPRRDPQAQFVHEAQAGDAALEAMAGGAGSSIGKGGMITKILAAKRAAGSGAST
VIAWGREPDVLLRLARGESIGTLLVAQTQKNQARKQWMVDHLQLRGSVTVDAGAAAKLREDGKSLLPIGMVAVEGDFVRG
DVIAVRDAGGTEIARGLANYASAEARLLCRKPSAEFERLLGYAAEPEMVHRDNMVVLGAR

Sequences:

>Translated_380_residues
MVSSVLRDARRIVVKVGSSLVTNEGRGLDEVAIGEWCRQLAALVRGQGGEPREVIMVSSGAIAEGMKRLGWSSRPGEIHE
LQAAAAVGQMGLAQMYETKLREQSMGSAQVLLTHADLADRERYLNARSTLLTLLRLGVVPVINENDTVVTDEIKFGDNDT
LGALVANLVEADALVILTDQKGLYTADPRRDPQAQFVHEAQAGDAALEAMAGGAGSSIGKGGMITKILAAKRAAGSGAST
VIAWGREPDVLLRLARGESIGTLLVAQTQKNQARKQWMVDHLQLRGSVTVDAGAAAKLREDGKSLLPIGMVAVEGDFVRG
DVIAVRDAGGTEIARGLANYASAEARLLCRKPSAEFERLLGYAAEPEMVHRDNMVVLGAR
>Mature_380_residues
MVSSVLRDARRIVVKVGSSLVTNEGRGLDEVAIGEWCRQLAALVRGQGGEPREVIMVSSGAIAEGMKRLGWSSRPGEIHE
LQAAAAVGQMGLAQMYETKLREQSMGSAQVLLTHADLADRERYLNARSTLLTLLRLGVVPVINENDTVVTDEIKFGDNDT
LGALVANLVEADALVILTDQKGLYTADPRRDPQAQFVHEAQAGDAALEAMAGGAGSSIGKGGMITKILAAKRAAGSGAST
VIAWGREPDVLLRLARGESIGTLLVAQTQKNQARKQWMVDHLQLRGSVTVDAGAAAKLREDGKSLLPIGMVAVEGDFVRG
DVIAVRDAGGTEIARGLANYASAEARLLCRKPSAEFERLLGYAAEPEMVHRDNMVVLGAR

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=299, Percent_Identity=33.4448160535117, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI62912457, Length=297, Percent_Identity=33.6700336700337, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1786437, Length=370, Percent_Identity=44.8648648648649, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17569967, Length=279, Percent_Identity=32.9749103942652, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=32.9749103942652, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=31.7307692307692, Blast_Score=186, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=30.622009569378, Blast_Score=176, Evalue=4e-45,
Organism=Drosophila melanogaster, GI21357643, Length=305, Percent_Identity=33.4426229508197, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_ACIET (B9MDZ8)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002552252.1
- GeneID:   7383713
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_0773
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40366; Mature: 40366

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSVLRDARRIVVKVGSSLVTNEGRGLDEVAIGEWCRQLAALVRGQGGEPREVIMVSSG
CHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
AIAEGMKRLGWSSRPGEIHELQAAAAVGQMGLAQMYETKLREQSMGSAQVLLTHADLADR
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHH
ERYLNARSTLLTLLRLGVVPVINENDTVVTDEIKFGDNDTLGALVANLVEADALVILTDQ
HHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECC
KGLYTADPRRDPQAQFVHEAQAGDAALEAMAGGAGSSIGKGGMITKILAAKRAAGSGAST
CCCEECCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCE
VIAWGREPDVLLRLARGESIGTLLVAQTQKNQARKQWMVDHLQLRGSVTVDAGAAAKLRE
EEEECCCHHHEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
DGKSLLPIGMVAVEGDFVRGDVIAVRDAGGTEIARGLANYASAEARLLCRKPSAEFERLL
CCCCCCEEEEEEEECCEEECCEEEEECCCCHHHHHHHHHHHCHHHEEEECCCCHHHHHHH
GYAAEPEMVHRDNMVVLGAR
CCCCCCCEEECCCEEEEECC
>Mature Secondary Structure
MVSSVLRDARRIVVKVGSSLVTNEGRGLDEVAIGEWCRQLAALVRGQGGEPREVIMVSSG
CHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
AIAEGMKRLGWSSRPGEIHELQAAAAVGQMGLAQMYETKLREQSMGSAQVLLTHADLADR
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHH
ERYLNARSTLLTLLRLGVVPVINENDTVVTDEIKFGDNDTLGALVANLVEADALVILTDQ
HHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECC
KGLYTADPRRDPQAQFVHEAQAGDAALEAMAGGAGSSIGKGGMITKILAAKRAAGSGAST
CCCEECCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCE
VIAWGREPDVLLRLARGESIGTLLVAQTQKNQARKQWMVDHLQLRGSVTVDAGAAAKLRE
EEEECCCHHHEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
DGKSLLPIGMVAVEGDFVRGDVIAVRDAGGTEIARGLANYASAEARLLCRKPSAEFERLL
CCCCCCEEEEEEEECCEEECCEEEEECCCCHHHHHHHHHHHCHHHEEEECCCCHHHHHHH
GYAAEPEMVHRDNMVVLGAR
CCCCCCCEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA