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The map label for this gene is menE [H]

Identifier: 222109848

GI number: 222109848

Start: 652614

End: 654212

Strand: Direct

Name: menE [H]

Synonym: Dtpsy_0632

Alternate gene names: 222109848

Gene position: 652614-654212 (Clockwise)

Preceding gene: 222109845

Following gene: 222109849

Centisome position: 17.19

GC content: 70.23

Gene sequence:

>1599_bases
ATGAACATTGCCCACCTGTTGCAGCGCAGCGCGCTGGTGCATGGCGAGCGGCCCGCGCTGCTGGCCGGCACATGCCTGCT
GCACGACTACCGTACCCTTGCCGCGCGCGTGGCCGCGCTGGCCGCACACCTGCGCGCGCATTGCGGCGTGCAGCCGGGCG
AATGCGTGGCCATCTTCTCGGCCAATGGTCCGCAGTATCTGGAGGCGCTGCACGCCATCCACTGGTGCGGCGCCATCTCG
GTGCCCGTCAACTACAAGCTGCATGCGCGCGAGCTGGCTTACGTGCTTGCCGACAGCGGCGCGCGCGTGGTGTGCGTGTC
TGCGGCGCTGCACGATGCGGCGCTGGCTGCGGGCGCCGACGCCGCGGCGGTGCAGGTGTTGGGGGCATCCGCCTGGGAGC
AGGCGGCGCAGGGCGCGCCGCTGGCGCTGCAGGAGCGCGCACCCGACGACGTGGCCTCGCTGTTCTACACCTCGGGCACC
ACCGGGCGGCCCAAGGGGGTGATGCAGACGCACCGCAACCTGCTGGCCATGACCATGGCCTACTTCACCGACGTGGACGA
CGTGCAGCCCGGCGACGCCATGGTCTATGCCGCGCCCATGTCGCATGGCGCGGGCTTGTACAACTACGCCCACATGCTGC
GCGGCGCGCGCCACGTGGTGCCCGCGTCCGGCGGGTTCGACCCGGCCGAGCTGGTGCAATTGGCCGCGGACGTGGGCCGC
CTGTCGCTGTTTGCCGCGCCAACCATGGTGCACCGGCTGGTGGAGCACGTGCGCCGCACGGGCGCCAATGTGGACGGCTT
CAAAACCATCGTCTACGGCGGCGGTCCCATGTATGTGGACGACCTGCGCCGCGCCATCGACACCATGGGCCAGAAGTTCG
TGCAGATCTATGGCCAGGGCGAAAGCCCCATGACCATCACCGCGCTGGCGCGCGAGCACCTGGCCGACACGGCGCACCCG
CGCTGGGCCGAGCGCATCGCCTCGGTCGGCGTGGCGCATGCGTGCGTACAGGTGCGGGTGGTGGATGACGAGGACCGACC
CGTGCCCACGGGCGAACTGGGCGAAGTGGTGGTGCGCGGCGACACCGTGATGGCCGGCTACTGGCGCAATCCCGAGGCGT
CTGCGCAGACGCTGCGCGGCGGCTGGTTGCACACGGGCGATGTCGGCAGCCTGGACGCGCACGGGTTTCTGACCCTGCGC
GACCGCAGCAAGGACGTGATCATCTCGGGCGGCTCCAACATCTACCCGCGCGAGGTGGAGGAGGTGCTGCTGCTGCACCC
GCGCGTGAGCGAGGTGGCCGTGGTGGGCCAGCACGATGCCGACTGGGGCGAGGTGGTGGTGGCCTTCCTGGTGGCGGGCG
AGGGCGGCGCCGTGGACGATGCCGCGCTGCATGCGAAGCTCGATGCGGAACTTGACGCGGAACTTGACGCGGAACTTGAC
GCGGAACTTGACGCTTTGTGCCTGGACCACATCGCGCGCTTCAAGCGCCCCAAGGCCTACCGCTGGGTGCCGGCCCTGCC
CAAGAACAGCTATGGCAAGGTGCTCAAGACCGAGCTGCGCGCATGGCTGCAGGCCGAAGGGGCGGGGAGCGGGGCGTAA

Upstream 100 bases:

>100_bases
GTGCTCATCCCCCGCCAGGGATTTCCCTTGTGTATTTCTGCGACTGTGCGCGCTGCCGACGCTTGCCTATAGTCGCACCA
GGAGACAACGCGACAACCCC

Downstream 100 bases:

>100_bases
CAAACCCGGGAGCGGCGCGGACATTGCGCCGCCCATACCATGGGCGCTTTGCCCCGGCTGGAGCCCTTGCCATGAACCAA
CCCGTCTCCCTCAAGATCGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MNIAHLLQRSALVHGERPALLAGTCLLHDYRTLAARVAALAAHLRAHCGVQPGECVAIFSANGPQYLEALHAIHWCGAIS
VPVNYKLHARELAYVLADSGARVVCVSAALHDAALAAGADAAAVQVLGASAWEQAAQGAPLALQERAPDDVASLFYTSGT
TGRPKGVMQTHRNLLAMTMAYFTDVDDVQPGDAMVYAAPMSHGAGLYNYAHMLRGARHVVPASGGFDPAELVQLAADVGR
LSLFAAPTMVHRLVEHVRRTGANVDGFKTIVYGGGPMYVDDLRRAIDTMGQKFVQIYGQGESPMTITALAREHLADTAHP
RWAERIASVGVAHACVQVRVVDDEDRPVPTGELGEVVVRGDTVMAGYWRNPEASAQTLRGGWLHTGDVGSLDAHGFLTLR
DRSKDVIISGGSNIYPREVEEVLLLHPRVSEVAVVGQHDADWGEVVVAFLVAGEGGAVDDAALHAKLDAELDAELDAELD
AELDALCLDHIARFKRPKAYRWVPALPKNSYGKVLKTELRAWLQAEGAGSGA

Sequences:

>Translated_532_residues
MNIAHLLQRSALVHGERPALLAGTCLLHDYRTLAARVAALAAHLRAHCGVQPGECVAIFSANGPQYLEALHAIHWCGAIS
VPVNYKLHARELAYVLADSGARVVCVSAALHDAALAAGADAAAVQVLGASAWEQAAQGAPLALQERAPDDVASLFYTSGT
TGRPKGVMQTHRNLLAMTMAYFTDVDDVQPGDAMVYAAPMSHGAGLYNYAHMLRGARHVVPASGGFDPAELVQLAADVGR
LSLFAAPTMVHRLVEHVRRTGANVDGFKTIVYGGGPMYVDDLRRAIDTMGQKFVQIYGQGESPMTITALAREHLADTAHP
RWAERIASVGVAHACVQVRVVDDEDRPVPTGELGEVVVRGDTVMAGYWRNPEASAQTLRGGWLHTGDVGSLDAHGFLTLR
DRSKDVIISGGSNIYPREVEEVLLLHPRVSEVAVVGQHDADWGEVVVAFLVAGEGGAVDDAALHAKLDAELDAELDAELD
AELDALCLDHIARFKRPKAYRWVPALPKNSYGKVLKTELRAWLQAEGAGSGA
>Mature_532_residues
MNIAHLLQRSALVHGERPALLAGTCLLHDYRTLAARVAALAAHLRAHCGVQPGECVAIFSANGPQYLEALHAIHWCGAIS
VPVNYKLHARELAYVLADSGARVVCVSAALHDAALAAGADAAAVQVLGASAWEQAAQGAPLALQERAPDDVASLFYTSGT
TGRPKGVMQTHRNLLAMTMAYFTDVDDVQPGDAMVYAAPMSHGAGLYNYAHMLRGARHVVPASGGFDPAELVQLAADVGR
LSLFAAPTMVHRLVEHVRRTGANVDGFKTIVYGGGPMYVDDLRRAIDTMGQKFVQIYGQGESPMTITALAREHLADTAHP
RWAERIASVGVAHACVQVRVVDDEDRPVPTGELGEVVVRGDTVMAGYWRNPEASAQTLRGGWLHTGDVGSLDAHGFLTLR
DRSKDVIISGGSNIYPREVEEVLLLHPRVSEVAVVGQHDADWGEVVVAFLVAGEGGAVDDAALHAKLDAELDAELDAELD
AELDALCLDHIARFKRPKAYRWVPALPKNSYGKVLKTELRAWLQAEGAGSGA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=515, Percent_Identity=24.8543689320388, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI187761345, Length=558, Percent_Identity=27.7777777777778, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI187761343, Length=558, Percent_Identity=27.7777777777778, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI156151445, Length=384, Percent_Identity=27.8645833333333, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI157311624, Length=521, Percent_Identity=22.8406909788868, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI157311622, Length=521, Percent_Identity=22.8406909788868, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI58082049, Length=521, Percent_Identity=23.0326295585413, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI122937307, Length=533, Percent_Identity=25.328330206379, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI38505220, Length=505, Percent_Identity=24.7524752475248, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI115511026, Length=521, Percent_Identity=23.0326295585413, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI42794756, Length=485, Percent_Identity=23.0927835051546, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI42794760, Length=541, Percent_Identity=22.9205175600739, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI42794758, Length=541, Percent_Identity=22.9205175600739, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI40807491, Length=477, Percent_Identity=23.4800838574423, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI13375727, Length=542, Percent_Identity=24.5387453874539, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788107, Length=560, Percent_Identity=30, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI221142682, Length=541, Percent_Identity=26.6173752310536, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI145693145, Length=375, Percent_Identity=30.4, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788595, Length=514, Percent_Identity=27.0428015564202, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786810, Length=388, Percent_Identity=28.3505154639175, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786801, Length=479, Percent_Identity=26.0960334029228, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786529, Length=565, Percent_Identity=26.3716814159292, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=460, Percent_Identity=28.2608695652174, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71994694, Length=392, Percent_Identity=30.6122448979592, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71994690, Length=392, Percent_Identity=30.6122448979592, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71994703, Length=392, Percent_Identity=30.6122448979592, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32563687, Length=446, Percent_Identity=27.8026905829596, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17559526, Length=464, Percent_Identity=27.3706896551724, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17538037, Length=537, Percent_Identity=25.8845437616387, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=544, Percent_Identity=24.2647058823529, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=532, Percent_Identity=25.9398496240602, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=394, Percent_Identity=24.6192893401015, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17510401, Length=488, Percent_Identity=24.1803278688525, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17541856, Length=471, Percent_Identity=23.5668789808917, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=538, Percent_Identity=23.4200743494424, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=331, Percent_Identity=25.0755287009063, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=390, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17564090, Length=460, Percent_Identity=21.5217391304348, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI193204819, Length=218, Percent_Identity=26.605504587156, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=502, Percent_Identity=23.9043824701195, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24581924, Length=540, Percent_Identity=26.2962962962963, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI18859661, Length=541, Percent_Identity=27.3567467652495, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21355181, Length=516, Percent_Identity=23.8372093023256, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922652, Length=299, Percent_Identity=30.7692307692308, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281366413, Length=461, Percent_Identity=24.7288503253796, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24666501, Length=461, Percent_Identity=24.7288503253796, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24666497, Length=461, Percent_Identity=24.7288503253796, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20130357, Length=379, Percent_Identity=23.4828496042216, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648260, Length=373, Percent_Identity=26.0053619302949, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21356947, Length=442, Percent_Identity=22.8506787330317, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648253, Length=463, Percent_Identity=22.8941684665227, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24656500, Length=537, Percent_Identity=23.8361266294227, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648255, Length=463, Percent_Identity=22.6781857451404, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356441, Length=337, Percent_Identity=24.3323442136499, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21358303, Length=366, Percent_Identity=22.4043715846995, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648257, Length=455, Percent_Identity=23.5164835164835, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653035, Length=324, Percent_Identity=23.4567901234568, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281365686, Length=367, Percent_Identity=22.6158038147139, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI161076582, Length=332, Percent_Identity=22.5903614457831, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21355225, Length=512, Percent_Identity=23.4375, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921316, Length=190, Percent_Identity=30, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 56760; Mature: 56760

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAHLLQRSALVHGERPALLAGTCLLHDYRTLAARVAALAAHLRAHCGVQPGECVAIFS
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
ANGPQYLEALHAIHWCGAISVPVNYKLHARELAYVLADSGARVVCVSAALHDAALAAGAD
CCCHHHHHHHHHHHHCCEEECCCCCEEHHHHHHHHEECCCCEEEEEEHHHHHHHHHCCCC
AAAVQVLGASAWEQAAQGAPLALQERAPDDVASLFYTSGTTGRPKGVMQTHRNLLAMTMA
HHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
YFTDVDDVQPGDAMVYAAPMSHGAGLYNYAHMLRGARHVVPASGGFDPAELVQLAADVGR
HHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHH
LSLFAAPTMVHRLVEHVRRTGANVDGFKTIVYGGGPMYVDDLRRAIDTMGQKFVQIYGQG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHCCC
ESPMTITALAREHLADTAHPRWAERIASVGVAHACVQVRVVDDEDRPVPTGELGEVVVRG
CCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCEEEEC
DTVMAGYWRNPEASAQTLRGGWLHTGDVGSLDAHGFLTLRDRSKDVIISGGSNIYPREVE
CEEEEECCCCCCHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHH
EVLLLHPRVSEVAVVGQHDADWGEVVVAFLVAGEGGAVDDAALHAKLDAELDAELDAELD
HHHHCCCCHHHEEEECCCCCCHHHHHHHHHEECCCCCCCHHHHHEEECCCCCCCCCCCCC
AELDALCLDHIARFKRPKAYRWVPALPKNSYGKVLKTELRAWLQAEGAGSGA
CHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNIAHLLQRSALVHGERPALLAGTCLLHDYRTLAARVAALAAHLRAHCGVQPGECVAIFS
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
ANGPQYLEALHAIHWCGAISVPVNYKLHARELAYVLADSGARVVCVSAALHDAALAAGAD
CCCHHHHHHHHHHHHCCEEECCCCCEEHHHHHHHHEECCCCEEEEEEHHHHHHHHHCCCC
AAAVQVLGASAWEQAAQGAPLALQERAPDDVASLFYTSGTTGRPKGVMQTHRNLLAMTMA
HHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
YFTDVDDVQPGDAMVYAAPMSHGAGLYNYAHMLRGARHVVPASGGFDPAELVQLAADVGR
HHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHH
LSLFAAPTMVHRLVEHVRRTGANVDGFKTIVYGGGPMYVDDLRRAIDTMGQKFVQIYGQG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHCCC
ESPMTITALAREHLADTAHPRWAERIASVGVAHACVQVRVVDDEDRPVPTGELGEVVVRG
CCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCEEEEC
DTVMAGYWRNPEASAQTLRGGWLHTGDVGSLDAHGFLTLRDRSKDVIISGGSNIYPREVE
CEEEEECCCCCCHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHH
EVLLLHPRVSEVAVVGQHDADWGEVVVAFLVAGEGGAVDDAALHAKLDAELDAELDAELD
HHHHCCCCHHHEEEECCCCCCHHHHHHHHHEECCCCCCCHHHHHEEECCCCCCCCCCCCC
AELDALCLDHIARFKRPKAYRWVPALPKNSYGKVLKTELRAWLQAEGAGSGA
CHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA