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The map label for this gene is glmU

Identifier: 222109831

GI number: 222109831

Start: 634191

End: 635621

Strand: Direct

Name: glmU

Synonym: Dtpsy_0615

Alternate gene names: 222109831

Gene position: 634191-635621 (Clockwise)

Preceding gene: 222109830

Following gene: 222109832

Centisome position: 16.7

GC content: 69.6

Gene sequence:

>1431_bases
ATGACAGCACTGGACATCATCATCATGGCCGCGGGCAAGGGCACGCGCATGAAAAGCCGCATTCCCAAGGTCTTGCAACG
CCTGGCCGGCCGGCCCCTGCTGCACCATGTGCTGGGCCAGGCCGCCAGCCTGCAGGCGCGCCGCGTGGTGGTGGTAACGG
GCCATGGCGCTACAGAAGTAGAAGCTGCTTGCGCAGGATTGGCGGGCGCTGGAGGCACTTTTGACTTGAAATTCGTGCGC
CAGGAGCCGCAGTTGGGCACGGGCCACGCGGTGCAGCAGGCCACGCCCGCGCTGGCGGGCGATGGCACGGTGGTGGTGTT
GTCGGGCGACGTGCCGTTGACCCAGGCCGCGACGCTGCGCGCGCTGGTCGAGGCCGGGGCGGGCGAGCGCCTTGCGCTGC
TCACCGTGCGCCTGCCCAATCCCACAGGGTACGGCCGCATCGTGCGCGGCGAGGGCGGAACGGTGCAGCGCATCGTGGAG
CACAAGGATGCAAACGATGCCGAGCGCGCCATCGACGAGGTCTACAGCGGCATCATGGCCGTGCCCGCGCAGCGCCTGGC
GGGCTGGCTGGCGCGTCTGACCAACGACAACGCGCAGGGCGAGTACTACCTGACCGACATCGTGTCCATGGCTGTGGCGG
ACGGCGTGCCCGTGGCGGCCCACTGCATTGGCGATGCGCTGCAGGTGGCGGGCGTGAACAGCCCCGCGCAACTGGCCGAG
CTGGAGCGCGCGCACCAGCGCGCCCAGGCCGCGGCACTCATGGAGCAGGGCGTTCGCCTGGCCGACCCCGCGCGCTTTGA
CCTGCGCGACGATGCGCGCAGCGGCGCGCGCGGCGAAATCCTCTGTGCGCAGGATGTGGAGATCGACGTGGGCTGCATCT
TCACAGGCCGCGTGGAACTGGGCGAGGGTGCGCGCATCGGCGCCTATTGCCACATCAGCAACGCCACCATCGCCGCGGGC
GCTGTGGTCCACCCCTTTACCCACATCGATGGCGAGAAGGCCGGCGCCCATGTGGGCGAAGGCGCACTGATTGGTCCCTT
CGCGCGCCTGCGCCCCGGCGCGCAATTGGGGCGCGAGGTGCACATTGGCAACTTCGTGGAGGTGAAGAACAGCACGCTGG
CCGACGGCGCCAAGGCCAACCACCTGGCCTACCTGGGCGACGCCAGCGTGGGCGAGCGCGTGAACTACGGCGCCGGCAGC
ATCACCGCCAATTACGACGGTGCCAACAAGCACCGCACGGTGATCGAGGCCGACGTGCACATCGGCAGCAACTGCGTGCT
GGTGGCGCCCGTCACCATTGGGGCGGGCGGCACGGTGGGTGGCGGCTCCACCATCACCAAGGACACGCCACCCGGGGGGT
TGAGCGTGGCGCGTGGCCGGCAGGTGAGCATCGCGAACTGGAAGCGCCCCGCCAAACAGGCCAAGGGTTGA

Upstream 100 bases:

>100_bases
GCCGCGCCGCAGAGGGGCTGCGCATGGGCGGCGGGATAATGGCGGCGGGCTGCCGGCCTGGGCTGGCAGGACACGACGAC
CTTCCACTCTCCACACCTTC

Downstream 100 bases:

>100_bases
GGCCATGACCGGCGAGGGCATTCCACCGGCGGACACGCTGGCGCAACTGCGCAGCGAACTGGCCCAGGCGCGCCAGCAGA
TCAGCGATATGGCGGCGGCG

Product: UDP-N-acetylglucosamine pyrophosphorylase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTALDIIIMAAGKGTRMKSRIPKVLQRLAGRPLLHHVLGQAASLQARRVVVVTGHGATEVEAACAGLAGAGGTFDLKFVR
QEPQLGTGHAVQQATPALAGDGTVVVLSGDVPLTQAATLRALVEAGAGERLALLTVRLPNPTGYGRIVRGEGGTVQRIVE
HKDANDAERAIDEVYSGIMAVPAQRLAGWLARLTNDNAQGEYYLTDIVSMAVADGVPVAAHCIGDALQVAGVNSPAQLAE
LERAHQRAQAAALMEQGVRLADPARFDLRDDARSGARGEILCAQDVEIDVGCIFTGRVELGEGARIGAYCHISNATIAAG
AVVHPFTHIDGEKAGAHVGEGALIGPFARLRPGAQLGREVHIGNFVEVKNSTLADGAKANHLAYLGDASVGERVNYGAGS
ITANYDGANKHRTVIEADVHIGSNCVLVAPVTIGAGGTVGGGSTITKDTPPGGLSVARGRQVSIANWKRPAKQAKG

Sequences:

>Translated_476_residues
MTALDIIIMAAGKGTRMKSRIPKVLQRLAGRPLLHHVLGQAASLQARRVVVVTGHGATEVEAACAGLAGAGGTFDLKFVR
QEPQLGTGHAVQQATPALAGDGTVVVLSGDVPLTQAATLRALVEAGAGERLALLTVRLPNPTGYGRIVRGEGGTVQRIVE
HKDANDAERAIDEVYSGIMAVPAQRLAGWLARLTNDNAQGEYYLTDIVSMAVADGVPVAAHCIGDALQVAGVNSPAQLAE
LERAHQRAQAAALMEQGVRLADPARFDLRDDARSGARGEILCAQDVEIDVGCIFTGRVELGEGARIGAYCHISNATIAAG
AVVHPFTHIDGEKAGAHVGEGALIGPFARLRPGAQLGREVHIGNFVEVKNSTLADGAKANHLAYLGDASVGERVNYGAGS
ITANYDGANKHRTVIEADVHIGSNCVLVAPVTIGAGGTVGGGSTITKDTPPGGLSVARGRQVSIANWKRPAKQAKG
>Mature_475_residues
TALDIIIMAAGKGTRMKSRIPKVLQRLAGRPLLHHVLGQAASLQARRVVVVTGHGATEVEAACAGLAGAGGTFDLKFVRQ
EPQLGTGHAVQQATPALAGDGTVVVLSGDVPLTQAATLRALVEAGAGERLALLTVRLPNPTGYGRIVRGEGGTVQRIVEH
KDANDAERAIDEVYSGIMAVPAQRLAGWLARLTNDNAQGEYYLTDIVSMAVADGVPVAAHCIGDALQVAGVNSPAQLAEL
ERAHQRAQAAALMEQGVRLADPARFDLRDDARSGARGEILCAQDVEIDVGCIFTGRVELGEGARIGAYCHISNATIAAGA
VVHPFTHIDGEKAGAHVGEGALIGPFARLRPGAQLGREVHIGNFVEVKNSTLADGAKANHLAYLGDASVGERVNYGAGSI
TANYDGANKHRTVIEADVHIGSNCVLVAPVTIGAGGTVGGGSTITKDTPPGGLSVARGRQVSIANWKRPAKQAKG

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=472, Percent_Identity=45.9745762711864, Blast_Score=400, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_ACIET (B9MD63)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002552095.1
- ProteinModelPortal:   B9MD63
- GeneID:   7382989
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_0615
- ProtClustDB:   CLSK951126
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 49140; Mature: 49009

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 381-381 BINDING 81-81 BINDING 145-145 BINDING 160-160 BINDING 405-405 BINDING 423-423 BINDING 441-441 BINDING 458-458

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALDIIIMAAGKGTRMKSRIPKVLQRLAGRPLLHHVLGQAASLQARRVVVVTGHGATEV
CCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHCCCCEEEEEEECCCCCHH
EAACAGLAGAGGTFDLKFVRQEPQLGTGHAVQQATPALAGDGTVVVLSGDVPLTQAATLR
HHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHH
ALVEAGAGERLALLTVRLPNPTGYGRIVRGEGGTVQRIVEHKDANDAERAIDEVYSGIMA
HHHHCCCCCEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
VPAQRLAGWLARLTNDNAQGEYYLTDIVSMAVADGVPVAAHCIGDALQVAGVNSPAQLAE
HHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHH
LERAHQRAQAAALMEQGVRLADPARFDLRDDARSGARGEILCAQDVEIDVGCIFTGRVEL
HHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHCCCCCCCEEEECCCEEEEEEEEECCEEE
GEGARIGAYCHISNATIAAGAVVHPFTHIDGEKAGAHVGEGALIGPFARLRPGAQLGREV
CCCCEEEEEEEECCCEEECCCEECCHHCCCCCCCCCCCCCCCEECCHHHCCCCHHHCCEE
HIGNFVEVKNSTLADGAKANHLAYLGDASVGERVNYGAGSITANYDGANKHRTVIEADVH
ECCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEE
IGSNCVLVAPVTIGAGGTVGGGSTITKDTPPGGLSVARGRQVSIANWKRPAKQAKG
ECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCEEEECCCCCCHHHCCC
>Mature Secondary Structure 
TALDIIIMAAGKGTRMKSRIPKVLQRLAGRPLLHHVLGQAASLQARRVVVVTGHGATEV
CCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHCCCCEEEEEEECCCCCHH
EAACAGLAGAGGTFDLKFVRQEPQLGTGHAVQQATPALAGDGTVVVLSGDVPLTQAATLR
HHHHHCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHH
ALVEAGAGERLALLTVRLPNPTGYGRIVRGEGGTVQRIVEHKDANDAERAIDEVYSGIMA
HHHHCCCCCEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
VPAQRLAGWLARLTNDNAQGEYYLTDIVSMAVADGVPVAAHCIGDALQVAGVNSPAQLAE
HHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHH
LERAHQRAQAAALMEQGVRLADPARFDLRDDARSGARGEILCAQDVEIDVGCIFTGRVEL
HHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHCCCCCCCEEEECCCEEEEEEEEECCEEE
GEGARIGAYCHISNATIAAGAVVHPFTHIDGEKAGAHVGEGALIGPFARLRPGAQLGREV
CCCCEEEEEEEECCCEEECCCEECCHHCCCCCCCCCCCCCCCEECCHHHCCCCHHHCCEE
HIGNFVEVKNSTLADGAKANHLAYLGDASVGERVNYGAGSITANYDGANKHRTVIEADVH
ECCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEE
IGSNCVLVAPVTIGAGGTVGGGSTITKDTPPGGLSVARGRQVSIANWKRPAKQAKG
ECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCEEEECCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA