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The map label for this gene is tyrS [H]

Identifier: 222109827

GI number: 222109827

Start: 630414

End: 631646

Strand: Direct

Name: tyrS [H]

Synonym: Dtpsy_0611

Alternate gene names: 222109827

Gene position: 630414-631646 (Clockwise)

Preceding gene: 222109824

Following gene: 222109830

Centisome position: 16.6

GC content: 64.07

Gene sequence:

>1233_bases
ATGAATCAATCTGCTGTTACAACATTCCCGGTGACCGATGGTGTAGGACGGGCGCTTGAAGTCTCCCTGCGCGGGGTCGA
CGAGCTTCTGCCCCAAGAAGAATGGACCCAGAAACTTGCGCGTTCGGAGGCCACGGGCCAGCCGCTGCGCATCAAGCTCG
GGCTGGACCCCACGGCGCCGGACATCCACCTGGGCCATACGGTGGTGCTCAACAAGATGCGCCAATTGCAGGACCTGGGC
CACCAGGTCATCTTCCTGATCGGCGACTTCACCACGCTGATCGGCGACCCATCGGGCCGCAACAGCACGCGCCCGCCGCT
GACGGCCGAGCAGATCAAGGTCAACGCCGAAACCTACTACACCCAGGCGGCCAAGGTGCTGGACCCTGCACGCACCGAGA
TCCGCTACAACAGCGAGTGGTGCGACCAGTTGGGTGCGCGCGGCATGATTCAGCTGTCGGCCAAGTACACGGTGGCACGC
ATGATGGAGCGCAACGATTTCCACCAGCGCTTCACCGACGGCAGCTCCATCAGCCTGCACGAGTTCCTCTACCCGCTACT
GCAGGGCTATGACTCGGTAGCGCTCAGGAGCGACCTGGAGCTGGGCGGCACTGATCAGAAGTTCAACCTGCTCATGGGAC
GCCATCTGCAGCAAGAATGGGGGCAGGAGCCGCAGTGTGTGCTCACCATGCCGCTGCTGGTGGGGCTGGATGGCGTGGAC
AAGATGTCCAAGTCCAAGAACAACTACATCGGCATCACGGAGGACGCCAACTCCATGTTCGCCAAGGTGCTCTCCATTTC
GGACACCCTGATGTGGGACTGGTACACGCTGCTGTCGTTCAAGAGCCTGGGCGAAATCGCTGCGCTCAAGGCCGAGGTGG
CGGCAGGCCGCAATCCCAAGGATGCCAAGGTGATGCTGGCCAAGGAGATCACCGCGCGCTTTCACAGCGCCGCGCTGGCC
GACGCGGCCGAGCAGGATTTCGTCAACCGCAGCAAGGGCGGCATCCCCGACGACATTCCCGAAGTCCGCCTGGCGGGCGC
GCCCCTGGGCATCGGTGCGCTGCTCAAGCAGGCCAACCTTGCGCCCTCGACCAGCGAGGCCAACCGCCTCATCGACGGCG
GCGGCGTGCGCGTGGACGGCGTCGTGGTCAGCGACAAGGGCCTGAAGCTGGAAGCGGGCAGCTACGTGGTGCAGGTCGGC
AAGCGCAAGTTCGCGCGCGTCACCTTGGCCTGA

Upstream 100 bases:

>100_bases
GAGCGAACGCCGGGCGTACGGGTGGCCCACTAGAATCCACGGCTGGAAAGTGCCCGGAAGTATAGTCGTTGGGGCCCTGG
CGGCACCCCAAAAAACCCTT

Downstream 100 bases:

>100_bases
CGCCCGGCGGGGCGCGACCGCGTTCCCGCTTTGCCAAGCCACTCCGCGCAGTGGCTTTTTTCATGCCCTTTCAGGCCAGG
GCGGCGGTGCGTTGCGCCTG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNQSAVTTFPVTDGVGRALEVSLRGVDELLPQEEWTQKLARSEATGQPLRIKLGLDPTAPDIHLGHTVVLNKMRQLQDLG
HQVIFLIGDFTTLIGDPSGRNSTRPPLTAEQIKVNAETYYTQAAKVLDPARTEIRYNSEWCDQLGARGMIQLSAKYTVAR
MMERNDFHQRFTDGSSISLHEFLYPLLQGYDSVALRSDLELGGTDQKFNLLMGRHLQQEWGQEPQCVLTMPLLVGLDGVD
KMSKSKNNYIGITEDANSMFAKVLSISDTLMWDWYTLLSFKSLGEIAALKAEVAAGRNPKDAKVMLAKEITARFHSAALA
DAAEQDFVNRSKGGIPDDIPEVRLAGAPLGIGALLKQANLAPSTSEANRLIDGGGVRVDGVVVSDKGLKLEAGSYVVQVG
KRKFARVTLA

Sequences:

>Translated_410_residues
MNQSAVTTFPVTDGVGRALEVSLRGVDELLPQEEWTQKLARSEATGQPLRIKLGLDPTAPDIHLGHTVVLNKMRQLQDLG
HQVIFLIGDFTTLIGDPSGRNSTRPPLTAEQIKVNAETYYTQAAKVLDPARTEIRYNSEWCDQLGARGMIQLSAKYTVAR
MMERNDFHQRFTDGSSISLHEFLYPLLQGYDSVALRSDLELGGTDQKFNLLMGRHLQQEWGQEPQCVLTMPLLVGLDGVD
KMSKSKNNYIGITEDANSMFAKVLSISDTLMWDWYTLLSFKSLGEIAALKAEVAAGRNPKDAKVMLAKEITARFHSAALA
DAAEQDFVNRSKGGIPDDIPEVRLAGAPLGIGALLKQANLAPSTSEANRLIDGGGVRVDGVVVSDKGLKLEAGSYVVQVG
KRKFARVTLA
>Mature_410_residues
MNQSAVTTFPVTDGVGRALEVSLRGVDELLPQEEWTQKLARSEATGQPLRIKLGLDPTAPDIHLGHTVVLNKMRQLQDLG
HQVIFLIGDFTTLIGDPSGRNSTRPPLTAEQIKVNAETYYTQAAKVLDPARTEIRYNSEWCDQLGARGMIQLSAKYTVAR
MMERNDFHQRFTDGSSISLHEFLYPLLQGYDSVALRSDLELGGTDQKFNLLMGRHLQQEWGQEPQCVLTMPLLVGLDGVD
KMSKSKNNYIGITEDANSMFAKVLSISDTLMWDWYTLLSFKSLGEIAALKAEVAAGRNPKDAKVMLAKEITARFHSAALA
DAAEQDFVNRSKGGIPDDIPEVRLAGAPLGIGALLKQANLAPSTSEANRLIDGGGVRVDGVVVSDKGLKLEAGSYVVQVG
KRKFARVTLA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=406, Percent_Identity=26.3546798029557, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1787925, Length=403, Percent_Identity=28.287841191067, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25145802, Length=209, Percent_Identity=30.1435406698565, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=335, Percent_Identity=23.5820895522388, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI20130383, Length=401, Percent_Identity=26.1845386533666, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44851; Mature: 44851

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSAVTTFPVTDGVGRALEVSLRGVDELLPQEEWTQKLARSEATGQPLRIKLGLDPTAP
CCCCCEEEECCCCCCCCEEEEHHHCHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCC
DIHLGHTVVLNKMRQLQDLGHQVIFLIGDFTTLIGDPSGRNSTRPPLTAEQIKVNAETYY
CCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHHCCCCCCCCCCCCCCHHHEEECHHHHH
TQAAKVLDPARTEIRYNSEWCDQLGARGMIQLSAKYTVARMMERNDFHQRFTDGSSISLH
HHHHHHHCCHHHHEEECHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHH
EFLYPLLQGYDSVALRSDLELGGTDQKFNLLMGRHLQQEWGQEPQCVLTMPLLVGLDGVD
HHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCHH
KMSKSKNNYIGITEDANSMFAKVLSISDTLMWDWYTLLSFKSLGEIAALKAEVAAGRNPK
HHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAKVMLAKEITARFHSAALADAAEQDFVNRSKGGIPDDIPEVRLAGAPLGIGALLKQANL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHCCC
APSTSEANRLIDGGGVRVDGVVVSDKGLKLEAGSYVVQVGKRKFARVTLA
CCCCHHHHHEECCCCEEEEEEEECCCCCEEECCHHHHHHCCHHHEEEECC
>Mature Secondary Structure
MNQSAVTTFPVTDGVGRALEVSLRGVDELLPQEEWTQKLARSEATGQPLRIKLGLDPTAP
CCCCCEEEECCCCCCCCEEEEHHHCHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCC
DIHLGHTVVLNKMRQLQDLGHQVIFLIGDFTTLIGDPSGRNSTRPPLTAEQIKVNAETYY
CCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHHCCCCCCCCCCCCCCHHHEEECHHHHH
TQAAKVLDPARTEIRYNSEWCDQLGARGMIQLSAKYTVARMMERNDFHQRFTDGSSISLH
HHHHHHHCCHHHHEEECHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHH
EFLYPLLQGYDSVALRSDLELGGTDQKFNLLMGRHLQQEWGQEPQCVLTMPLLVGLDGVD
HHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCHH
KMSKSKNNYIGITEDANSMFAKVLSISDTLMWDWYTLLSFKSLGEIAALKAEVAAGRNPK
HHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAKVMLAKEITARFHSAALADAAEQDFVNRSKGGIPDDIPEVRLAGAPLGIGALLKQANL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHCCC
APSTSEANRLIDGGGVRVDGVVVSDKGLKLEAGSYVVQVGKRKFARVTLA
CCCCHHHHHEECCCCEEEEEEEECCCCCEEECCHHHHHHCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA