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The map label for this gene is slt [H]

Identifier: 222109741

GI number: 222109741

Start: 541765

End: 543738

Strand: Direct

Name: slt [H]

Synonym: Dtpsy_0525

Alternate gene names: 222109741

Gene position: 541765-543738 (Clockwise)

Preceding gene: 222109736

Following gene: 222109749

Centisome position: 14.27

GC content: 70.21

Gene sequence:

>1974_bases
ATGAAGATTCTGACACCGCTGATCGCCGGCGCTGCCCTGGCCACCGCCGCGCCGTGGGCCGGGGCGCAAAACCGCGGCGA
TGACACCGTCCTGGAGATGCAGAAAGCCTTCCGCAAGGGCGACAAGCAGCGGCTTGCCGCGCTGCTGCCGGGCGCGCGCG
GCCATGCCTTGGAGCCCTGGGCTGCGTACTGGGAACTGCGTGCGCGGCTGGAGGAGGCCCAACCCTCCGAAGTGACCGCC
TTCCTGCAGCGCTGGGCGGGCACCTACCAGGAAGACCGCCTGCGCAACGACTGGCTGCTGCTGCTGGGCCAACGCCGCGA
CTGGGACCAATTCGCAGAATTTCACCCGCAGTTCCGCATGGGCGATGACCGCGAGGTGCATTGCTACGCGCTCGCGATCG
AGCAGATCAAGGGCACGGCCGCCGAGAGTGCCGGCGCCGAGATGCTGCGCCACTGGTATGCGCTGCGCGAGCTGGACGAC
GGCTGCTCCAGTGCCGCCGCCGAGATGCTGGCCGCCAAGAAAATCAAGCCCCTGGACGTGTGGCGCAAGGCGCGTCTGGG
CGCAGAGGCCAACCGCCTGCGCGTGGTGCGCAAGGCGGTGGAAATCGTCGCGCCCGATGCGCTGCCGCAACTGCGCGAGG
CGTTGGATTCGCCCACCAAGTACCTCACGGGCCGCGCCACGGCGCGTGGCAAGGAGCGTCAGGAACTGGTGGTGCTGGCG
CTCACCCGCATGGCCATGAGCGATGTCGCCAACGCCGCCAGCCTGCTGGACAGCAAATGGGGGCCGCACCTGTCGGCCGA
GGAGCGCAACTGGCTCTGGGGCCTGATCGGCAAGCAGGCCGCCACGGCGCTGTCGCCCGATGCGCTCAGCTACTACGCCA
ACGTGGGCCGTGACGCCGACCTGACCGACGACATGCTGGCCTGGCGCACGCGCGCCGCGCTGCGGGCCGGGCAATGGAAG
CTGGTGGGCCGCAGCATCGATGCCATGAGCCCCGCGGCGCGCCAGGAAAGCGTCTGGACGTACTGGAAGGCGCGCGCCCT
CATGGCCAACCGCCCCGGCGACGAGGATCGCGCGGCCGCGCGCCAGCTGTATGCGCGCATCGCCGGCACCACGGGTTTTT
ATGAACAGCTGGCGCTGGAGGAGCTGGGCGAGCGCGTCACGCAGCCCCCGGCGCCCGCACCGCTGACCGAGGAAGAAAAA
GCCACCGCGCGCGCCAACCCCGGCCTGAACCGTGCGCTGTACGCCATCCTGATGGGCCTGCGCAGCGAAGGCGTGCGCGA
GTGGAACTACACCACCAACCTGCACACCCCTGGCGGCATGGGCGAGCGCGAGCTGCTGGCGGCGGCCGACTTCGCCTGCC
AGCGCGAGGTGTGGGACCGCTGCATCAACACCAGCGAGCGCACCAAGGGCGTGATCGACGCGGCGCAGCGCTTTCCGATG
CCGTTCCGCTCCGCCGTGGTGGAGCGCGCGCGGGGCATCGGGCTGGACCCGGCCTATGTCTATGGCCTGATCCGCCAGGA
AAGCCGCTTCATCATGGATGCCCGCTCGGGCGTGGGCGCGTCGGGCCTGATGCAGGTGATGCCCGCCACCGCGCGCTGGA
CGGCGCGCAAGATCGGCCTGGAGGGCTTCTCCCCCCAGCAGCTCTACGACCGCGACACCAACATCACCATCGGCACCGCC
TATCTGAAGCTGGCGCTGGACGACTTCGACGGTTCCATGCCCCTGGCGGCGGCAGCCTACAACGCCGGCCCGGGCCGCCC
GCGCAACTGGCGCAACGGCCCGGTGCTGGACGCGGCCATCTGGGCCGAGAACGTGCCCTTCAGCGAGACGCGCGACTATG
TGAAGAAGGTGCTGGCCAACACGGTGAACTACGCCGCCATCCTCACCGGCCAGCCGCAGTCGCTCAAGAGCCGGCTGGGC
ACCGTGGGCCCGCGCGACGCGCGCGCGCCGGAGCCGAACAAGGATCTGCCTTGA

Upstream 100 bases:

>100_bases
TGGTCGGCGCGCTGCAGGCGGTCCGGCAGATTCAGGCGCTGTTCTACCAATGCGCGGCGCAGGGCTGCTTTGTCCATCAC
ATAATCCCCCAATGCTTTGG

Downstream 100 bases:

>100_bases
ATCTGCCTTGAGGGCCTGCTGCGCTATATAAAAAGGAGCTTTTAGCGCTTGCTGGATAAGCGCTGGAGCCATTTTTCATG
CCACATCGTCACCCCGCGGC

Product: lytic transglycosylase subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MKILTPLIAGAALATAAPWAGAQNRGDDTVLEMQKAFRKGDKQRLAALLPGARGHALEPWAAYWELRARLEEAQPSEVTA
FLQRWAGTYQEDRLRNDWLLLLGQRRDWDQFAEFHPQFRMGDDREVHCYALAIEQIKGTAAESAGAEMLRHWYALRELDD
GCSSAAAEMLAAKKIKPLDVWRKARLGAEANRLRVVRKAVEIVAPDALPQLREALDSPTKYLTGRATARGKERQELVVLA
LTRMAMSDVANAASLLDSKWGPHLSAEERNWLWGLIGKQAATALSPDALSYYANVGRDADLTDDMLAWRTRAALRAGQWK
LVGRSIDAMSPAARQESVWTYWKARALMANRPGDEDRAAARQLYARIAGTTGFYEQLALEELGERVTQPPAPAPLTEEEK
ATARANPGLNRALYAILMGLRSEGVREWNYTTNLHTPGGMGERELLAAADFACQREVWDRCINTSERTKGVIDAAQRFPM
PFRSAVVERARGIGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWTARKIGLEGFSPQQLYDRDTNITIGTA
YLKLALDDFDGSMPLAAAAYNAGPGRPRNWRNGPVLDAAIWAENVPFSETRDYVKKVLANTVNYAAILTGQPQSLKSRLG
TVGPRDARAPEPNKDLP

Sequences:

>Translated_657_residues
MKILTPLIAGAALATAAPWAGAQNRGDDTVLEMQKAFRKGDKQRLAALLPGARGHALEPWAAYWELRARLEEAQPSEVTA
FLQRWAGTYQEDRLRNDWLLLLGQRRDWDQFAEFHPQFRMGDDREVHCYALAIEQIKGTAAESAGAEMLRHWYALRELDD
GCSSAAAEMLAAKKIKPLDVWRKARLGAEANRLRVVRKAVEIVAPDALPQLREALDSPTKYLTGRATARGKERQELVVLA
LTRMAMSDVANAASLLDSKWGPHLSAEERNWLWGLIGKQAATALSPDALSYYANVGRDADLTDDMLAWRTRAALRAGQWK
LVGRSIDAMSPAARQESVWTYWKARALMANRPGDEDRAAARQLYARIAGTTGFYEQLALEELGERVTQPPAPAPLTEEEK
ATARANPGLNRALYAILMGLRSEGVREWNYTTNLHTPGGMGERELLAAADFACQREVWDRCINTSERTKGVIDAAQRFPM
PFRSAVVERARGIGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWTARKIGLEGFSPQQLYDRDTNITIGTA
YLKLALDDFDGSMPLAAAAYNAGPGRPRNWRNGPVLDAAIWAENVPFSETRDYVKKVLANTVNYAAILTGQPQSLKSRLG
TVGPRDARAPEPNKDLP
>Mature_657_residues
MKILTPLIAGAALATAAPWAGAQNRGDDTVLEMQKAFRKGDKQRLAALLPGARGHALEPWAAYWELRARLEEAQPSEVTA
FLQRWAGTYQEDRLRNDWLLLLGQRRDWDQFAEFHPQFRMGDDREVHCYALAIEQIKGTAAESAGAEMLRHWYALRELDD
GCSSAAAEMLAAKKIKPLDVWRKARLGAEANRLRVVRKAVEIVAPDALPQLREALDSPTKYLTGRATARGKERQELVVLA
LTRMAMSDVANAASLLDSKWGPHLSAEERNWLWGLIGKQAATALSPDALSYYANVGRDADLTDDMLAWRTRAALRAGQWK
LVGRSIDAMSPAARQESVWTYWKARALMANRPGDEDRAAARQLYARIAGTTGFYEQLALEELGERVTQPPAPAPLTEEEK
ATARANPGLNRALYAILMGLRSEGVREWNYTTNLHTPGGMGERELLAAADFACQREVWDRCINTSERTKGVIDAAQRFPM
PFRSAVVERARGIGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWTARKIGLEGFSPQQLYDRDTNITIGTA
YLKLALDDFDGSMPLAAAAYNAGPGRPRNWRNGPVLDAAIWAENVPFSETRDYVKKVLANTVNYAAILTGQPQSLKSRLG
TVGPRDARAPEPNKDLP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=659, Percent_Identity=25.3414264036419, Blast_Score=158, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72721; Mature: 72721

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILTPLIAGAALATAAPWAGAQNRGDDTVLEMQKAFRKGDKQRLAALLPGARGHALEPW
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHH
AAYWELRARLEEAQPSEVTAFLQRWAGTYQEDRLRNDWLLLLGQRRDWDQFAEFHPQFRM
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCHHHHHHHCCCCCC
GDDREVHCYALAIEQIKGTAAESAGAEMLRHWYALRELDDGCSSAAAEMLAAKKIKPLDV
CCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
WRKARLGAEANRLRVVRKAVEIVAPDALPQLREALDSPTKYLTGRATARGKERQELVVLA
HHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHCCCHHHCCCHHHHHHHHH
LTRMAMSDVANAASLLDSKWGPHLSAEERNWLWGLIGKQAATALSPDALSYYANVGRDAD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
LTDDMLAWRTRAALRAGQWKLVGRSIDAMSPAARQESVWTYWKARALMANRPGDEDRAAA
CCHHHHHHHHHHHHHCCCEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RQLYARIAGTTGFYEQLALEELGERVTQPPAPAPLTEEEKATARANPGLNRALYAILMGL
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
RSEGVREWNYTTNLHTPGGMGERELLAAADFACQREVWDRCINTSERTKGVIDAAQRFPM
HHCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
PFRSAVVERARGIGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWTARKIGL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCC
EGFSPQQLYDRDTNITIGTAYLKLALDDFDGSMPLAAAAYNAGPGRPRNWRNGPVLDAAI
CCCCHHHHHCCCCCEEEEEHEEEEEHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEHHH
WAENVPFSETRDYVKKVLANTVNYAAILTGQPQSLKSRLGTVGPRDARAPEPNKDLP
HHCCCCCHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKILTPLIAGAALATAAPWAGAQNRGDDTVLEMQKAFRKGDKQRLAALLPGARGHALEPW
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHH
AAYWELRARLEEAQPSEVTAFLQRWAGTYQEDRLRNDWLLLLGQRRDWDQFAEFHPQFRM
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCHHHHHHHCCCCCC
GDDREVHCYALAIEQIKGTAAESAGAEMLRHWYALRELDDGCSSAAAEMLAAKKIKPLDV
CCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
WRKARLGAEANRLRVVRKAVEIVAPDALPQLREALDSPTKYLTGRATARGKERQELVVLA
HHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHCCCHHHCCCHHHHHHHHH
LTRMAMSDVANAASLLDSKWGPHLSAEERNWLWGLIGKQAATALSPDALSYYANVGRDAD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
LTDDMLAWRTRAALRAGQWKLVGRSIDAMSPAARQESVWTYWKARALMANRPGDEDRAAA
CCHHHHHHHHHHHHHCCCEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RQLYARIAGTTGFYEQLALEELGERVTQPPAPAPLTEEEKATARANPGLNRALYAILMGL
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
RSEGVREWNYTTNLHTPGGMGERELLAAADFACQREVWDRCINTSERTKGVIDAAQRFPM
HHCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
PFRSAVVERARGIGLDPAYVYGLIRQESRFIMDARSGVGASGLMQVMPATARWTARKIGL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCC
EGFSPQQLYDRDTNITIGTAYLKLALDDFDGSMPLAAAAYNAGPGRPRNWRNGPVLDAAI
CCCCHHHHHCCCCCEEEEEHEEEEEHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEHHH
WAENVPFSETRDYVKKVLANTVNYAAILTGQPQSLKSRLGTVGPRDARAPEPNKDLP
HHCCCCCHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]