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The map label for this gene is tag [H]

Identifier: 222109668

GI number: 222109668

Start: 460928

End: 461626

Strand: Direct

Name: tag [H]

Synonym: Dtpsy_0448

Alternate gene names: 222109668

Gene position: 460928-461626 (Clockwise)

Preceding gene: 222109666

Following gene: 222109671

Centisome position: 12.14

GC content: 68.81

Gene sequence:

>699_bases
ATGCGACCCAACCCCCACAATGCCCCCATGCTCGAAACCACCGAACCCTCCTCCCCAGACGAAGCCAGTGGCCTGTTCAC
CGACGAGGGCGGCCATGCGCGCTGCTTCTGGTGCGGCGCCAGCCCGCTGTACCAGCACTACCACGACCACGAATGGGGCT
TCCCGGTGACGGACGAGCGCCGCCTGTTCGAGAAGCTCTGCCTGGAGGGGTTCCAGGCCGGCTTGTCGTGGATCACCATC
CTGAACAAGCGCGAGGCCTTCCGCGCGGCGCTGGCGCAGTTCGAGGCCGAGCCGCTGGCGGACTTCGGCGCCGCCGAGGT
GGACCAACTGATGGGCAACGCCGGTATCGTGCGCCACCGCGGCAAGATCGAGTCGGCCATCAACAATGCGCGCCGCGTGC
TGGAGCTGCGCCGCGAGTTCGGGTCGCTGGCGCACTATGTGTGGCGCTATGCGCCCCTGGCCGCGCAGCAGCGGCCCGAG
CGCATGACGCTGCCTGCGGTGCGTGCGATGACCACCTCGGCGGCGTCGGTGGCGCTGTCCAAGGATTTGAAGAAGCGTGG
GTTCAGCTTCGTGGGTCCAACCACCATGTATGCCTTCATGCAGGCCATGGGGCTGGTCAACGACCACCTGGAGGGCTGCG
GCGCACGCGCTGCGGCAGAGGCCGCGCGCGCCGCGTTCACACCCCCCGCTGCGGGCTGA

Upstream 100 bases:

>100_bases
GCGGCTGCCAGCAGTGGCCACGGGCGCCGGGGCGATGGGGTGGATGTGCTCATGGTGGCGGAGGCGGGTGCGGCCAAGCA
TAGCAAGACGTTCTTGCGCC

Downstream 100 bases:

>100_bases
TGCGCGCCCCAAAGCCGGCTTAAGGCCTGCTCACGCTATTCGCACTGGCAAAAATAGTGTGAACAGGCCCTAAACCACCG
CGTCGGACCGGACCACGATG

Product: DNA-3-methyladenine glycosylase i

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase; TAG [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MRPNPHNAPMLETTEPSSPDEASGLFTDEGGHARCFWCGASPLYQHYHDHEWGFPVTDERRLFEKLCLEGFQAGLSWITI
LNKREAFRAALAQFEAEPLADFGAAEVDQLMGNAGIVRHRGKIESAINNARRVLELRREFGSLAHYVWRYAPLAAQQRPE
RMTLPAVRAMTTSAASVALSKDLKKRGFSFVGPTTMYAFMQAMGLVNDHLEGCGARAAAEAARAAFTPPAAG

Sequences:

>Translated_232_residues
MRPNPHNAPMLETTEPSSPDEASGLFTDEGGHARCFWCGASPLYQHYHDHEWGFPVTDERRLFEKLCLEGFQAGLSWITI
LNKREAFRAALAQFEAEPLADFGAAEVDQLMGNAGIVRHRGKIESAINNARRVLELRREFGSLAHYVWRYAPLAAQQRPE
RMTLPAVRAMTTSAASVALSKDLKKRGFSFVGPTTMYAFMQAMGLVNDHLEGCGARAAAEAARAAFTPPAAG
>Mature_232_residues
MRPNPHNAPMLETTEPSSPDEASGLFTDEGGHARCFWCGASPLYQHYHDHEWGFPVTDERRLFEKLCLEGFQAGLSWITI
LNKREAFRAALAQFEAEPLADFGAAEVDQLMGNAGIVRHRGKIESAINNARRVLELRREFGSLAHYVWRYAPLAAQQRPE
RMTLPAVRAMTTSAASVALSKDLKKRGFSFVGPTTMYAFMQAMGLVNDHLEGCGARAAAEAARAAFTPPAAG

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=181, Percent_Identity=48.6187845303867, Blast_Score=182, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 25536; Mature: 25536

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNPHNAPMLETTEPSSPDEASGLFTDEGGHARCFWCGASPLYQHYHDHEWGFPVTDER
CCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCHHHHHHCCCCCCCCCCHHH
RLFEKLCLEGFQAGLSWITILNKREAFRAALAQFEAEPLADFGAAEVDQLMGNAGIVRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCCCHHHHH
GKIESAINNARRVLELRREFGSLAHYVWRYAPLAAQQRPERMTLPAVRAMTTSAASVALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
KDLKKRGFSFVGPTTMYAFMQAMGLVNDHLEGCGARAAAEAARAAFTPPAAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRPNPHNAPMLETTEPSSPDEASGLFTDEGGHARCFWCGASPLYQHYHDHEWGFPVTDER
CCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCHHHHHHCCCCCCCCCCHHH
RLFEKLCLEGFQAGLSWITILNKREAFRAALAQFEAEPLADFGAAEVDQLMGNAGIVRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCCCHHHHH
GKIESAINNARRVLELRREFGSLAHYVWRYAPLAAQQRPERMTLPAVRAMTTSAASVALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
KDLKKRGFSFVGPTTMYAFMQAMGLVNDHLEGCGARAAAEAARAAFTPPAAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]