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The map label for this gene is cynT [H]

Identifier: 222109652

GI number: 222109652

Start: 444861

End: 445538

Strand: Direct

Name: cynT [H]

Synonym: Dtpsy_0432

Alternate gene names: 222109652

Gene position: 444861-445538 (Clockwise)

Preceding gene: 222109651

Following gene: 222109653

Centisome position: 11.72

GC content: 68.58

Gene sequence:

>678_bases
ATGCCTGACGACGAGCTGCTGCAACGGCTGCGCCGCTTCCATCTGGATGCCTTCCCCCAATACCGCGAGCAATTCCAGAC
GCTCGTGGACGAGGGGCAACACCCGACGACCCTGTTCATCGGGTGCTCGGATTCCCGTCTGCTGCCCTACCTGCTGACGG
GGGCAGGACCGGGCGAGCTGTTCCTGGTGCGCAACGTGGGGGCCTTCGTGCCGCCCTACGACGGCTCCTACGGCCACCAT
GGCACGGCCGCCGCCATCGAGTTCGCGGTGCTCGAACTGCATGTGAGCCGCATCGTCGTGTGCGGCCACAGCCACTGCGG
CGCCATCAAGGCGCTGTATGGCGAGGTCTCGCCCGAGGCGCGCAACCTGCAGCGCTGGCTGGACCTGGGCCGTGAGGCCG
CGCTGCCCGTGCAGCCCGGCCCCGAAGCCCTGCGCCGCACCGAGCAGCGCGCCGTGGTGCTGCAACTGGAACGCCTCATG
GAGTACCCCATGGTGCGCCGCCGCGTCGAGGACGGCAGCATTGCGCTGCATGGCTGGCACTACGTGATCGAGGAGGGCGA
GGTCCACGTGTTCGACGTGGCCACCGGCGGCTTCGTGGCGGCGTCCAAGGCCAAGAGCAGCGGCACGGGCCCGTATGCGC
CGTATGTGGAACATGATGGGCAAGTGCTGGTGGACTGA

Upstream 100 bases:

>100_bases
GTACCTGCAGCTGCTGGCGGGCGAGGCCGAGGTCGAGGGCGTGCACCTCGATGCGGCCGATACGCCGCTGGTGCAGTAGC
GCCAGGCAGGGGAGACACCC

Downstream 100 bases:

>100_bases
GGTGCACGCTGGCCACACTGGGCGAGGCCCACGCCCATGACTGAAGTGCGGCGCCGCACCGTGCCCCTGCGCAGCGAGCC
CGCCGCGCCGGGCCGGCCCT

Product: carbonate dehydratase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MPDDELLQRLRRFHLDAFPQYREQFQTLVDEGQHPTTLFIGCSDSRLLPYLLTGAGPGELFLVRNVGAFVPPYDGSYGHH
GTAAAIEFAVLELHVSRIVVCGHSHCGAIKALYGEVSPEARNLQRWLDLGREAALPVQPGPEALRRTEQRAVVLQLERLM
EYPMVRRRVEDGSIALHGWHYVIEEGEVHVFDVATGGFVAASKAKSSGTGPYAPYVEHDGQVLVD

Sequences:

>Translated_225_residues
MPDDELLQRLRRFHLDAFPQYREQFQTLVDEGQHPTTLFIGCSDSRLLPYLLTGAGPGELFLVRNVGAFVPPYDGSYGHH
GTAAAIEFAVLELHVSRIVVCGHSHCGAIKALYGEVSPEARNLQRWLDLGREAALPVQPGPEALRRTEQRAVVLQLERLM
EYPMVRRRVEDGSIALHGWHYVIEEGEVHVFDVATGGFVAASKAKSSGTGPYAPYVEHDGQVLVD
>Mature_224_residues
PDDELLQRLRRFHLDAFPQYREQFQTLVDEGQHPTTLFIGCSDSRLLPYLLTGAGPGELFLVRNVGAFVPPYDGSYGHHG
TAAAIEFAVLELHVSRIVVCGHSHCGAIKALYGEVSPEARNLQRWLDLGREAALPVQPGPEALRRTEQRAVVLQLERLME
YPMVRRRVEDGSIALHGWHYVIEEGEVHVFDVATGGFVAASKAKSSGTGPYAPYVEHDGQVLVD

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=201, Percent_Identity=41.2935323383085, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786318, Length=171, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI115532990, Length=245, Percent_Identity=26.530612244898, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25147564, Length=217, Percent_Identity=26.2672811059908, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324292, Length=177, Percent_Identity=30.5084745762712, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 24940; Mature: 24808

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDDELLQRLRRFHLDAFPQYREQFQTLVDEGQHPTTLFIGCSDSRLLPYLLTGAGPGEL
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCCCCE
FLVRNVGAFVPPYDGSYGHHGTAAAIEFAVLELHVSRIVVCGHSHCGAIKALYGEVSPEA
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCCCH
RNLQRWLDLGREAALPVQPGPEALRRTEQRAVVLQLERLMEYPMVRRRVEDGSIALHGWH
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEEE
YVIEEGEVHVFDVATGGFVAASKAKSSGTGPYAPYVEHDGQVLVD
EEEECCCEEEEEECCCCEEEECCCCCCCCCCCCCCEECCCEEEEC
>Mature Secondary Structure 
PDDELLQRLRRFHLDAFPQYREQFQTLVDEGQHPTTLFIGCSDSRLLPYLLTGAGPGEL
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCCCCE
FLVRNVGAFVPPYDGSYGHHGTAAAIEFAVLELHVSRIVVCGHSHCGAIKALYGEVSPEA
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCCCH
RNLQRWLDLGREAALPVQPGPEALRRTEQRAVVLQLERLMEYPMVRRRVEDGSIALHGWH
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEEE
YVIEEGEVHVFDVATGGFVAASKAKSSGTGPYAPYVEHDGQVLVD
EEEECCCEEEEEECCCCEEEECCCCCCCCCCCCCCEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]