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The map label for this gene is narV [H]

Identifier: 222109650

GI number: 222109650

Start: 443177

End: 443911

Strand: Direct

Name: narV [H]

Synonym: Dtpsy_0430

Alternate gene names: 222109650

Gene position: 443177-443911 (Clockwise)

Preceding gene: 222109649

Following gene: 222109651

Centisome position: 11.67

GC content: 65.31

Gene sequence:

>735_bases
ATGACCGCCTGGATCGACAACCTCTTGTTCGGCCTGTACCCCTACATCTGTCTGGCCGTTTTCTTCATCGGCAGCTGGGC
GCGCTTCGACCGCGACCAGTACACATGGAAGAGCGACTCCTCGCAGCTGCTGCGCCGCGGCAGCCTGCGCTGGGGCAGCA
ACCTGTTCCACGTGGGCGTGCTGGTCCTGTTCTTCGGCCACTTCGTGGGCATGCTCACGCCGCACGCGCTGTACGAGCCC
TTCATGAGCGCAGGCGCCAAGCAGATCATGGCCATGGTTGTTGGCGGCGTCGCCGGCACCCTGGGCTTCATCGGCGTGAC
GCTGCTGCTGCACCGCCGCCTGACGGATGCGCGTATCCGCGCCACCTCCAAGACCAGCGATATCCTGCTGCTGTGGGTGC
TGTGGGTGCAGTTGGCCCTGGGCCTGGCGACCATTCCGCTGTCGGCGCAGCACCTGGACGGCTCCATGATGATGCTGCTG
GCCGAATGGGCGCAACGCATCGTCACCTTCCGCGGCGGCGCCGTGGAACTGTTGGCCAATGCCGGCTGGGTGTTCAAGGC
GCACATGTTCCTGGGCATGACCGTGTTCCTGATCTTCCCGTTCACCCGCTTGGTGCACGTGTGGAGCGGGTTTGGCACAC
TGGCCTACATCACGCGCCCGTACCAACTGGTGCGCGCCCGCCGCATGAACCTGCCCGCAGGCCACAACCAGCCGGGCAAT
CGCAACGCGCTATGA

Upstream 100 bases:

>100_bases
TCGACGGCTGCGCCACCCGCGGCCAGAACCGCCCGGACCAGCCCCAACCCCTGCATTTCGTGCGCAACGCACGCACCAAC
TCGTCCCAAGGAGCCTCGGT

Downstream 100 bases:

>100_bases
AGTGAATAGCTACCAGCGCTTGCTGGTAGCGGGCTGGAGGCCAATTTCGCTTGAAATCGGCTTTCGGCCTCCTAGCCTCC
AGGAGAACCCCATGAGCAAC

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTAWIDNLLFGLYPYICLAVFFIGSWARFDRDQYTWKSDSSQLLRRGSLRWGSNLFHVGVLVLFFGHFVGMLTPHALYEP
FMSAGAKQIMAMVVGGVAGTLGFIGVTLLLHRRLTDARIRATSKTSDILLLWVLWVQLALGLATIPLSAQHLDGSMMMLL
AEWAQRIVTFRGGAVELLANAGWVFKAHMFLGMTVFLIFPFTRLVHVWSGFGTLAYITRPYQLVRARRMNLPAGHNQPGN
RNAL

Sequences:

>Translated_244_residues
MTAWIDNLLFGLYPYICLAVFFIGSWARFDRDQYTWKSDSSQLLRRGSLRWGSNLFHVGVLVLFFGHFVGMLTPHALYEP
FMSAGAKQIMAMVVGGVAGTLGFIGVTLLLHRRLTDARIRATSKTSDILLLWVLWVQLALGLATIPLSAQHLDGSMMMLL
AEWAQRIVTFRGGAVELLANAGWVFKAHMFLGMTVFLIFPFTRLVHVWSGFGTLAYITRPYQLVRARRMNLPAGHNQPGN
RNAL
>Mature_243_residues
TAWIDNLLFGLYPYICLAVFFIGSWARFDRDQYTWKSDSSQLLRRGSLRWGSNLFHVGVLVLFFGHFVGMLTPHALYEPF
MSAGAKQIMAMVVGGVAGTLGFIGVTLLLHRRLTDARIRATSKTSDILLLWVLWVQLALGLATIPLSAQHLDGSMMMLLA
EWAQRIVTFRGGAVELLANAGWVFKAHMFLGMTVFLIFPFTRLVHVWSGFGTLAYITRPYQLVRARRMNLPAGHNQPGNR
NAL

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transf

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=229, Percent_Identity=56.3318777292576, Blast_Score=257, Evalue=4e-70,
Organism=Escherichia coli, GI1787480, Length=225, Percent_Identity=54.2222222222222, Blast_Score=244, Evalue=5e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 27404; Mature: 27273

Theoretical pI: Translated: 11.15; Mature: 11.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCC
>Mature Secondary Structure 
TAWIDNLLFGLYPYICLAVFFIGSWARFDRDQYTWKSDSSQLLRRGSLRWGSNLFHVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHH
LVLFFGHFVGMLTPHALYEPFMSAGAKQIMAMVVGGVAGTLGFIGVTLLLHRRLTDARIR
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATSKTSDILLLWVLWVQLALGLATIPLSAQHLDGSMMMLLAEWAQRIVTFRGGAVELLAN
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHC
AGWVFKAHMFLGMTVFLIFPFTRLVHVWSGFGTLAYITRPYQLVRARRMNLPAGHNQPGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RNAL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]