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The map label for this gene is dmlR [H]

Identifier: 222109548

GI number: 222109548

Start: 338663

End: 339601

Strand: Direct

Name: dmlR [H]

Synonym: Dtpsy_0328

Alternate gene names: 222109548

Gene position: 338663-339601 (Clockwise)

Preceding gene: 222109542

Following gene: 222109549

Centisome position: 8.92

GC content: 70.82

Gene sequence:

>939_bases
ATGGACAAACTCAAAGCGTTCGAGTCATTCGTGTCGGTGGCCACGCGGGGCAGTCTCACCGCCGCCGCCAAGGCCGAGGG
CGTGGCCCCGGCCATCATGGGGCGGCGCCTGGACGCGCTGGAGGAGCACCTGGGCGTGAAGCTGCTGGTGCGCACCACGC
GGCGCATCACCCTCACGCACGAGGGCAGCGCCTTCCTGGAGGACTGCCAGCGCCTGCTGTCCGACGTGGCCAACGCCGAG
GCCAGCGTATCCGCTGGCGGCGTGAAGGCCACGGGGCATCTGCGCGTCACCGCACCCGCGGGCTTTGGCCGGCGCCATGT
GGCACCCCTGGTGCCGCGCTTTCATGACCTGCACCCCGAGGTGACCCTCTCGCTGAACCTGAGCGACCGCGTGGTGGATC
TGGCGGGCGAGGGCTACGACTGCGCCGTGCGCGTGGGCGACCTGCCCGACTCGTCCCTGGTCAGCGTGCGCATGGCGGAC
AACCGTCGCCTGTGCGTGGCCACGCCCCAGTACCTGCAGCGGCGCGGCACCCCCACCCACCCGGGCGAGTTGGCGCAGCA
CGACTGCCTGACCCTCTCCAGCGATGCCTCGCAAACGCGCGGCTGGGCGTTTCGCGTGCCGGTTGCCGGCGGTGGTGGTG
CCACCGAGGTCGTGCACTTCAAGCCGGGCGGGCCCCTCGATTGCTCTGACGGCCAGGTACTGCACGACTGGTGCCTGGGC
GGCTGGGGCATCGCCTGGCGCAGCACCTGGGAGGTGGAGTCCGAGATCGCGGCCGGCCGCCTGGTGGCGGTGCTGGAGGA
GTTCGCCGCGCCGCCCAACGGCATCTATGTCGTCTTTCCCCAGCGCAAGCACCTGCCGCTGCGCGTGCGGCTGTGGATCG
AGTACCTGAAGCACCATTACGCGCAGCCCGAATTCTGGGCACGGCCCGCCTCTGTCTGA

Upstream 100 bases:

>100_bases
TTGGCACACTCTGCATGGGGAATGCGGTGCATGCTTTCATGAAATTGATAGCTGCTTGCGCTTGCTGTATAAGGCTTGGA
GCCTGATTTGGCTTGTATTC

Downstream 100 bases:

>100_bases
CATGAACGCGGGGCACAATGCCGCGGCATTCCTCCCACCGGGCCGCCATCATGCCGGGGCCGTACGCACGGCCCACCGTA
CAGGAGCTTTCATGCGCATT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDKLKAFESFVSVATRGSLTAAAKAEGVAPAIMGRRLDALEEHLGVKLLVRTTRRITLTHEGSAFLEDCQRLLSDVANAE
ASVSAGGVKATGHLRVTAPAGFGRRHVAPLVPRFHDLHPEVTLSLNLSDRVVDLAGEGYDCAVRVGDLPDSSLVSVRMAD
NRRLCVATPQYLQRRGTPTHPGELAQHDCLTLSSDASQTRGWAFRVPVAGGGGATEVVHFKPGGPLDCSDGQVLHDWCLG
GWGIAWRSTWEVESEIAAGRLVAVLEEFAAPPNGIYVVFPQRKHLPLRVRLWIEYLKHHYAQPEFWARPASV

Sequences:

>Translated_312_residues
MDKLKAFESFVSVATRGSLTAAAKAEGVAPAIMGRRLDALEEHLGVKLLVRTTRRITLTHEGSAFLEDCQRLLSDVANAE
ASVSAGGVKATGHLRVTAPAGFGRRHVAPLVPRFHDLHPEVTLSLNLSDRVVDLAGEGYDCAVRVGDLPDSSLVSVRMAD
NRRLCVATPQYLQRRGTPTHPGELAQHDCLTLSSDASQTRGWAFRVPVAGGGGATEVVHFKPGGPLDCSDGQVLHDWCLG
GWGIAWRSTWEVESEIAAGRLVAVLEEFAAPPNGIYVVFPQRKHLPLRVRLWIEYLKHHYAQPEFWARPASV
>Mature_312_residues
MDKLKAFESFVSVATRGSLTAAAKAEGVAPAIMGRRLDALEEHLGVKLLVRTTRRITLTHEGSAFLEDCQRLLSDVANAE
ASVSAGGVKATGHLRVTAPAGFGRRHVAPLVPRFHDLHPEVTLSLNLSDRVVDLAGEGYDCAVRVGDLPDSSLVSVRMAD
NRRLCVATPQYLQRRGTPTHPGELAQHDCLTLSSDASQTRGWAFRVPVAGGGGATEVVHFKPGGPLDCSDGQVLHDWCLG
GWGIAWRSTWEVESEIAAGRLVAVLEEFAAPPNGIYVVFPQRKHLPLRVRLWIEYLKHHYAQPEFWARPASV

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=304, Percent_Identity=35.8552631578947, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=33.6769759450172, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=31.4189189189189, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1789440, Length=294, Percent_Identity=30.6122448979592, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1789639, Length=302, Percent_Identity=29.1390728476821, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787128, Length=298, Percent_Identity=25.8389261744966, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787589, Length=298, Percent_Identity=28.1879194630872, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789173, Length=276, Percent_Identity=27.536231884058, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790011, Length=313, Percent_Identity=23.3226837060703, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=24.2320819112628, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=25.4355400696864, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=23.972602739726, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33914; Mature: 33914

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLKAFESFVSVATRGSLTAAAKAEGVAPAIMGRRLDALEEHLGVKLLVRTTRRITLTH
CCHHHHHHHHHHHHHCCCEEEHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECEEEEEEE
EGSAFLEDCQRLLSDVANAEASVSAGGVKATGHLRVTAPAGFGRRHVAPLVPRFHDLHPE
CCCHHHHHHHHHHHHHHCCCCEECCCCEEEECEEEEEECCCCCCCCCCHHCCHHHCCCCC
VTLSLNLSDRVVDLAGEGYDCAVRVGDLPDSSLVSVRMADNRRLCVATPQYLQRRGTPTH
EEEEEECCCEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCEEEEECHHHHHHCCCCCC
PGELAQHDCLTLSSDASQTRGWAFRVPVAGGGGATEVVHFKPGGPLDCSDGQVLHDWCLG
CCHHHHCCCEEECCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCEEHHHHCC
GWGIAWRSTWEVESEIAAGRLVAVLEEFAAPPNGIYVVFPQRKHLPLRVRLWIEYLKHHY
CCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHC
AQPEFWARPASV
CCCHHCCCCCCC
>Mature Secondary Structure
MDKLKAFESFVSVATRGSLTAAAKAEGVAPAIMGRRLDALEEHLGVKLLVRTTRRITLTH
CCHHHHHHHHHHHHHCCCEEEHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECEEEEEEE
EGSAFLEDCQRLLSDVANAEASVSAGGVKATGHLRVTAPAGFGRRHVAPLVPRFHDLHPE
CCCHHHHHHHHHHHHHHCCCCEECCCCEEEECEEEEEECCCCCCCCCCHHCCHHHCCCCC
VTLSLNLSDRVVDLAGEGYDCAVRVGDLPDSSLVSVRMADNRRLCVATPQYLQRRGTPTH
EEEEEECCCEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCEEEEECHHHHHHCCCCCC
PGELAQHDCLTLSSDASQTRGWAFRVPVAGGGGATEVVHFKPGGPLDCSDGQVLHDWCLG
CCHHHHCCCEEECCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCEEHHHHCC
GWGIAWRSTWEVESEIAAGRLVAVLEEFAAPPNGIYVVFPQRKHLPLRVRLWIEYLKHHY
CCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHC
AQPEFWARPASV
CCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]