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The map label for this gene is braD [H]

Identifier: 222109394

GI number: 222109394

Start: 194448

End: 196355

Strand: Direct

Name: braD [H]

Synonym: Dtpsy_0173

Alternate gene names: 222109394

Gene position: 194448-196355 (Clockwise)

Preceding gene: 222109393

Following gene: 222109395

Centisome position: 5.12

GC content: 71.38

Gene sequence:

>1908_bases
ATGGATGTTTCGGGGCTGCTGGCCCAACTGCTCAATGGCCTGGCGGGCGCTTCCACGTTGTTCCTCGTGGCGGCGGGGCT
GTCGCTGATCTTTGGGGTGATGCGCATCGTCAACTTCGCGCACGGCTCGTTTTACATGCTGGGCCTGTACCTGGCCTATG
CCCTGGTGGAGCGTCTGGGCGATGCCCTCGGCTTCTGGCCTGCGCTGCTGCTGGCTCCGCTGGCCGTGGGCCTGCTGGGC
GCGGCGGTGGAGGTGCTGCTGCTGCGCCGCATCTACCGCGCACCCGAACTGCTGCAGCTGCTGGCCACCTTCGCCTTGGT
GCTGGTGATCAAGGACGCGGCGCTGTGGCTCTGGGGGCCGGAGGAGCTGTTCGGCCCGCGTGCGCCGGGCCTGGAGGGCG
CGGTGGACCTGCTGGGCCGGCGCTTCCCAGCCTACGACCTGTTCCTCATTGCCGTGGGGCCGCTGGTGCTGGTGCTGCTC
ACGCTGCTGCTCACGCGCACGCGCTGGGGCACGCTGGTGCGCGCCGCCACGCAGGACCGTGAGATGGTCGGTGCGCTGGG
TGTGAACCAGGCCTGGCTGTTCACCAGCGTGTTCGCGCTGGGGGCCCTGCTGGCAGGCCTGGGCGGCGCGCTGCAGCTGC
CGCGCGAGCCCGCCAGCCTGGAGCTGGACATGCTCACCATCGGCGCCGCCTTCGTGGTAGTGGTGGTGGGCGGCATGGGC
TCGCTGCCCGGCGCCTTCGTGGCGGCGTTGGTGATTGCCGAGCTCAAGGCCGTGTGCATCTGGCTGGGCGTGGTCGAGGT
CGCGGGGGTGGAGGTGGCCTTTCCCCGGCTCACGCTGGTGGTGGAGTTCCTGGTGATGGCGGCCGTGCTCATCTGGCGGC
CCTGGGGCTTCATGGGCCGCCCGCAGGCCGTGGCGCGTGGCGTGGGCACGCCCGAGGCGCCGCTGCGCCAGGCGGGGCAG
GGCGCCACGGCCGCCTGGTGCGCGTTGGTGCTGGCCCTGGCGCTGCTGCCGCTGGCGGTGGAGCCGGGCAGCTACGCCAC
CGTGTTGCTGACCGACATCGCGATTGCCGCGCTGTTCGCCGCCAGCCTGCATTGCATCCTGGGGCCGGGCGGGCTGCATT
CGTTCGGCCATGCGGCGTACTTCGGCCTGGGCGCCTACGGCGCGGCGCTGCTGGTGCGCTGGCTGGACTGGCCCATGGGC
ATGGCGCTGCTGCTGGGGCCGCTGGTGGCGGCGGCCGGCGCGCTGGTGTACGGCTGGTTCTGCGTGCGCCTGTCGGGCGT
GTCGCTCACCATGCTGACGCTGGCGTTCGCACAGATCACCTGGGCCATCGCCTACCAGTGGGACGGGTTCACCGGCGGCA
GCAACGGCATCACCGGCGTGTGGCCGCCGCAGTGGGCCGCGCAGGGCGCGGGCTTCTATTGGCTGGTGCTGGCGCTGGCC
GTGGCGGGCATCTACCTGCTGCGGCGCATGCTGTTTGCACCGCTGGGCTGGGCGCTGCGCGCGGCGCGCGACTCGGCGCT
GCGGGCCGACGCCATTGGCATCGACGTGCGCGGCGTGCAGTGGGCCGCCTTTGTGGTGGCAGGGCTGCTGGCCGGTCTGG
CGGGGGCGCTGTACGTGTTTTCCAAAGGCAGCGTGGCGCCCGATGCGCTGGGCGTGTCGCGCTCGGTGGACGGGCTGGTG
ATGGTGCTGCTGGGCGGCATGCAGCAGCTGGCCGGGCCGGTGGTGGGCGCGGGCGTGTTCACCTGGCTGCACGACACCGT
GGCGCGCAGTACCGACTACTGGCGCGCGCTGCTGGGCGGCATCATGCTGCTGCTGGTGCTGCTGTTTCCTCAAGGAATTT
CGGGGTTTGCGCAGGTGTGGAAAGCGCTGGCAGCTATCAAAAACAAAGCACAGGAGGGCGCGCCGTGA

Upstream 100 bases:

>100_bases
GGGGAACGCGCGGCCGCTACCGCGGGGCGACCCGGGCGCTATGGCTGCGGGCTTCAACCGCGCGGGCTGCGGTGGCCCTG
TGCGGCATTGGACGACTGAC

Downstream 100 bases:

>100_bases
GCCTGCTGCAGGTCCAGGAGCTGCGCAAGTCCTTCGGCGGAGTGCAGGCCGTGCAGGGCGTGTCGTTCGCACTGCAGGCC
GGCGAGTTGCTGGCGCTGAT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MDVSGLLAQLLNGLAGASTLFLVAAGLSLIFGVMRIVNFAHGSFYMLGLYLAYALVERLGDALGFWPALLLAPLAVGLLG
AAVEVLLLRRIYRAPELLQLLATFALVLVIKDAALWLWGPEELFGPRAPGLEGAVDLLGRRFPAYDLFLIAVGPLVLVLL
TLLLTRTRWGTLVRAATQDREMVGALGVNQAWLFTSVFALGALLAGLGGALQLPREPASLELDMLTIGAAFVVVVVGGMG
SLPGAFVAALVIAELKAVCIWLGVVEVAGVEVAFPRLTLVVEFLVMAAVLIWRPWGFMGRPQAVARGVGTPEAPLRQAGQ
GATAAWCALVLALALLPLAVEPGSYATVLLTDIAIAALFAASLHCILGPGGLHSFGHAAYFGLGAYGAALLVRWLDWPMG
MALLLGPLVAAAGALVYGWFCVRLSGVSLTMLTLAFAQITWAIAYQWDGFTGGSNGITGVWPPQWAAQGAGFYWLVLALA
VAGIYLLRRMLFAPLGWALRAARDSALRADAIGIDVRGVQWAAFVVAGLLAGLAGALYVFSKGSVAPDALGVSRSVDGLV
MVLLGGMQQLAGPVVGAGVFTWLHDTVARSTDYWRALLGGIMLLLVLLFPQGISGFAQVWKALAAIKNKAQEGAP

Sequences:

>Translated_635_residues
MDVSGLLAQLLNGLAGASTLFLVAAGLSLIFGVMRIVNFAHGSFYMLGLYLAYALVERLGDALGFWPALLLAPLAVGLLG
AAVEVLLLRRIYRAPELLQLLATFALVLVIKDAALWLWGPEELFGPRAPGLEGAVDLLGRRFPAYDLFLIAVGPLVLVLL
TLLLTRTRWGTLVRAATQDREMVGALGVNQAWLFTSVFALGALLAGLGGALQLPREPASLELDMLTIGAAFVVVVVGGMG
SLPGAFVAALVIAELKAVCIWLGVVEVAGVEVAFPRLTLVVEFLVMAAVLIWRPWGFMGRPQAVARGVGTPEAPLRQAGQ
GATAAWCALVLALALLPLAVEPGSYATVLLTDIAIAALFAASLHCILGPGGLHSFGHAAYFGLGAYGAALLVRWLDWPMG
MALLLGPLVAAAGALVYGWFCVRLSGVSLTMLTLAFAQITWAIAYQWDGFTGGSNGITGVWPPQWAAQGAGFYWLVLALA
VAGIYLLRRMLFAPLGWALRAARDSALRADAIGIDVRGVQWAAFVVAGLLAGLAGALYVFSKGSVAPDALGVSRSVDGLV
MVLLGGMQQLAGPVVGAGVFTWLHDTVARSTDYWRALLGGIMLLLVLLFPQGISGFAQVWKALAAIKNKAQEGAP
>Mature_635_residues
MDVSGLLAQLLNGLAGASTLFLVAAGLSLIFGVMRIVNFAHGSFYMLGLYLAYALVERLGDALGFWPALLLAPLAVGLLG
AAVEVLLLRRIYRAPELLQLLATFALVLVIKDAALWLWGPEELFGPRAPGLEGAVDLLGRRFPAYDLFLIAVGPLVLVLL
TLLLTRTRWGTLVRAATQDREMVGALGVNQAWLFTSVFALGALLAGLGGALQLPREPASLELDMLTIGAAFVVVVVGGMG
SLPGAFVAALVIAELKAVCIWLGVVEVAGVEVAFPRLTLVVEFLVMAAVLIWRPWGFMGRPQAVARGVGTPEAPLRQAGQ
GATAAWCALVLALALLPLAVEPGSYATVLLTDIAIAALFAASLHCILGPGGLHSFGHAAYFGLGAYGAALLVRWLDWPMG
MALLLGPLVAAAGALVYGWFCVRLSGVSLTMLTLAFAQITWAIAYQWDGFTGGSNGITGVWPPQWAAQGAGFYWLVLALA
VAGIYLLRRMLFAPLGWALRAARDSALRADAIGIDVRGVQWAAFVVAGLLAGLAGALYVFSKGSVAPDALGVSRSVDGLV
MVLLGGMQQLAGPVVGAGVFTWLHDTVARSTDYWRALLGGIMLLLVLLFPQGISGFAQVWKALAAIKNKAQEGAP

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=324, Percent_Identity=26.2345679012346, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789866, Length=317, Percent_Identity=24.2902208201893, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 66547; Mature: 66547

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSGLLAQLLNGLAGASTLFLVAAGLSLIFGVMRIVNFAHGSFYMLGLYLAYALVERLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DALGFWPALLLAPLAVGLLGAAVEVLLLRRIYRAPELLQLLATFALVLVIKDAALWLWGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECH
EELFGPRAPGLEGAVDLLGRRFPAYDLFLIAVGPLVLVLLTLLLTRTRWGTLVRAATQDR
HHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMVGALGVNQAWLFTSVFALGALLAGLGGALQLPREPASLELDMLTIGAAFVVVVVGGMG
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCC
SLPGAFVAALVIAELKAVCIWLGVVEVAGVEVAFPRLTLVVEFLVMAAVLIWRPWGFMGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PQAVARGVGTPEAPLRQAGQGATAAWCALVLALALLPLAVEPGSYATVLLTDIAIAALFA
CHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ASLHCILGPGGLHSFGHAAYFGLGAYGAALLVRWLDWPMGMALLLGPLVAAAGALVYGWF
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVRLSGVSLTMLTLAFAQITWAIAYQWDGFTGGSNGITGVWPPQWAAQGAGFYWLVLALA
HHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHH
VAGIYLLRRMLFAPLGWALRAARDSALRADAIGIDVRGVQWAAFVVAGLLAGLAGALYVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHH
SKGSVAPDALGVSRSVDGLVMVLLGGMQQLAGPVVGAGVFTWLHDTVARSTDYWRALLGG
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
IMLLLVLLFPQGISGFAQVWKALAAIKNKAQEGAP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDVSGLLAQLLNGLAGASTLFLVAAGLSLIFGVMRIVNFAHGSFYMLGLYLAYALVERLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DALGFWPALLLAPLAVGLLGAAVEVLLLRRIYRAPELLQLLATFALVLVIKDAALWLWGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECH
EELFGPRAPGLEGAVDLLGRRFPAYDLFLIAVGPLVLVLLTLLLTRTRWGTLVRAATQDR
HHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMVGALGVNQAWLFTSVFALGALLAGLGGALQLPREPASLELDMLTIGAAFVVVVVGGMG
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCC
SLPGAFVAALVIAELKAVCIWLGVVEVAGVEVAFPRLTLVVEFLVMAAVLIWRPWGFMGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PQAVARGVGTPEAPLRQAGQGATAAWCALVLALALLPLAVEPGSYATVLLTDIAIAALFA
CHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ASLHCILGPGGLHSFGHAAYFGLGAYGAALLVRWLDWPMGMALLLGPLVAAAGALVYGWF
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVRLSGVSLTMLTLAFAQITWAIAYQWDGFTGGSNGITGVWPPQWAAQGAGFYWLVLALA
HHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHH
VAGIYLLRRMLFAPLGWALRAARDSALRADAIGIDVRGVQWAAFVVAGLLAGLAGALYVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHH
SKGSVAPDALGVSRSVDGLVMVLLGGMQQLAGPVVGAGVFTWLHDTVARSTDYWRALLGG
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
IMLLLVLLFPQGISGFAQVWKALAAIKNKAQEGAP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]