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The map label for this gene is rutD [H]

Identifier: 222109311

GI number: 222109311

Start: 95923

End: 96705

Strand: Direct

Name: rutD [H]

Synonym: Dtpsy_0088

Alternate gene names: 222109311

Gene position: 95923-96705 (Clockwise)

Preceding gene: 222109309

Following gene: 222109312

Centisome position: 2.53

GC content: 73.56

Gene sequence:

>783_bases
ATGTCTCTTCTTCCACGCTTTTCCCTGCTCGGCTCCGGCCCTACCGTGTTGCTGCTGCACGATGCCGACGGCGATCACCT
GGGCTTTGCCCCGACGCTGGAAACCTTGGCCGCCGCGGGCTACCGCGCCGTGGCATGGGACATGCCGGGCTATGGCGGCA
GTGCGCCGGTGGAGCCCTACAACTTCAAGGGCCTGGCCCAGCGTGCGCTGCTGTTGCTCGATGCCCTGCGGTGCGAGCAG
GTGGTGCTGGTCGGCCACGGCCTGGGGGCGATGCTGGCGCTGGAGATCGCCGTGCGGGCACCGCTGCGCGTGCGGCGGCT
GGTGCTGTGTGCCGGCGGCCCGGCGCTGGACGAGGCCGCCACCGCCGTGTGGGTGGCGCCGCGCCTTGCGGGCTTGGACG
ATGGACGGGACATGGCCCGGCTGGCCGACCCTACCGTGGCGCGCGACGCCGGCCCCGGCGCGCTGCCCGAGGGGCTGCAG
CTTGCACGGCACGCCATGGGCCGCGTGCCGCCCGCCACCTACCGCCGTGCGTTGGAAGCCCTGACGGCTTTCGCGCGGCG
TGCGCCGGAACTTGTGCGCCTGCAGGTGCCCGCGCTGCTCGTCGGGGGTGAGTACGACCGCTGCACTCCGCCCGAGGCAC
TGCTCGCCCTGGCCCATGTGCTGCCCGATGCGCGCGCCGTGCTGCTGCCCGGCGTGGGCCACTGGCCGCAGCTTGAAGAC
CCCGAGGGTTTCGAGGCGGCGCTGCTGGACTTCCTGGCCGGCCCGGCGCCGGTGCTGCACTGA

Upstream 100 bases:

>100_bases
GGCGGCGCCCTGGGCGCGCCGCGTAGGGCAATGGGAGGGCTATTATCAGTCTGCACCAGCGCGCCTGCAGCGCGCATGCC
CTGCGGCCCTGGACCCTGCG

Downstream 100 bases:

>100_bases
ACGCGTGCGCGGTGATCCCTCACCGCAAATCGCTTACCCGCCCCTGACCGCGACCGCGCACCGCTGCGCAATAAACCACA
TCGCGCGCCCGCGCTTTTGG

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSLLPRFSLLGSGPTVLLLHDADGDHLGFAPTLETLAAAGYRAVAWDMPGYGGSAPVEPYNFKGLAQRALLLLDALRCEQ
VVLVGHGLGAMLALEIAVRAPLRVRRLVLCAGGPALDEAATAVWVAPRLAGLDDGRDMARLADPTVARDAGPGALPEGLQ
LARHAMGRVPPATYRRALEALTAFARRAPELVRLQVPALLVGGEYDRCTPPEALLALAHVLPDARAVLLPGVGHWPQLED
PEGFEAALLDFLAGPAPVLH

Sequences:

>Translated_260_residues
MSLLPRFSLLGSGPTVLLLHDADGDHLGFAPTLETLAAAGYRAVAWDMPGYGGSAPVEPYNFKGLAQRALLLLDALRCEQ
VVLVGHGLGAMLALEIAVRAPLRVRRLVLCAGGPALDEAATAVWVAPRLAGLDDGRDMARLADPTVARDAGPGALPEGLQ
LARHAMGRVPPATYRRALEALTAFARRAPELVRLQVPALLVGGEYDRCTPPEALLALAHVLPDARAVLLPGVGHWPQLED
PEGFEAALLDFLAGPAPVLH
>Mature_259_residues
SLLPRFSLLGSGPTVLLLHDADGDHLGFAPTLETLAAAGYRAVAWDMPGYGGSAPVEPYNFKGLAQRALLLLDALRCEQV
VLVGHGLGAMLALEIAVRAPLRVRRLVLCAGGPALDEAATAVWVAPRLAGLDDGRDMARLADPTVARDAGPGALPEGLQL
ARHAMGRVPPATYRRALEALTAFARRAPELVRLQVPALLVGGEYDRCTPPEALLALAHVLPDARAVLLPGVGHWPQLEDP
EGFEAALLDFLAGPAPVLH

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=297, Percent_Identity=24.9158249158249, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 27353; Mature: 27222

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLPRFSLLGSGPTVLLLHDADGDHLGFAPTLETLAAAGYRAVAWDMPGYGGSAPVEPY
CCCCCCHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
NFKGLAQRALLLLDALRCEQVVLVGHGLGAMLALEIAVRAPLRVRRLVLCAGGPALDEAA
CCHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCHHHEEEEEECCCCCHHHHH
TAVWVAPRLAGLDDGRDMARLADPTVARDAGPGALPEGLQLARHAMGRVPPATYRRALEA
HHHHHHHHHCCCCCCCHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
LTAFARRAPELVRLQVPALLVGGEYDRCTPPEALLALAHVLPDARAVLLPGVGHWPQLED
HHHHHHCCCHHEEEECCEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
PEGFEAALLDFLAGPAPVLH
CCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLLPRFSLLGSGPTVLLLHDADGDHLGFAPTLETLAAAGYRAVAWDMPGYGGSAPVEPY
CCCCCHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
NFKGLAQRALLLLDALRCEQVVLVGHGLGAMLALEIAVRAPLRVRRLVLCAGGPALDEAA
CCHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCHHHEEEEEECCCCCHHHHH
TAVWVAPRLAGLDDGRDMARLADPTVARDAGPGALPEGLQLARHAMGRVPPATYRRALEA
HHHHHHHHHCCCCCCCHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
LTAFARRAPELVRLQVPALLVGGEYDRCTPPEALLALAHVLPDARAVLLPGVGHWPQLED
HHHHHHCCCHHEEEECCEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
PEGFEAALLDFLAGPAPVLH
CCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA