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The map label for this gene is gyrB [H]

Identifier: 222109228

GI number: 222109228

Start: 2860

End: 5466

Strand: Direct

Name: gyrB [H]

Synonym: Dtpsy_0003

Alternate gene names: 222109228

Gene position: 2860-5466 (Clockwise)

Preceding gene: 222109227

Following gene: 222109230

Centisome position: 0.08

GC content: 66.55

Gene sequence:

>2607_bases
ATGACCGCAGAGCAATCGCAGCCCGCCCCCGAACAGCCCATTTCCGCCCCGGCCGAGCAGCCCGTCGCATCGGTCGCCGG
CAGCGACGGCTACGGCGAGGGCGCCATCCAGATCCTGGAGGGCCTGGAGGCCGTGCGCAAGCGTCCGGGCATGTACATCG
GCGATACGTCCGACGGCACCGGCCTGCACCACCTGGTCTTCGAGGTGGTGGACAACTCCATCGACGAGGCCCTGGCCGGC
CACTGCGACGACATCGTCGTCACCATCCACACCGACAACTCCATCAGCGTGGTGGACAACGGCCGCGGCATTCCCACCGG
CGTGAAGATGGACGACAAGCACGAGCCCAAGCGCTCGGCCGCCGAGATCGCCCTGACCGAGCTGCACGCCGGCGGCAAGT
TCAACCAGAACAGCTACAAGGTCTCGGGCGGCCTGCACGGCGTGGGCGTGTCCTGCGTGAACGCGCTGTCCAAGAAGCTG
CGCCTGACCGTGCGCCGCGACGGGCAGACGCACCAGCTGGAGTTCTCCAAGGGCGTGGTGCAGAACCGTCTCATCGAGAT
CGTGGATGGCTTCGAGGTCTCGCCCATGAAGGTCATCGGCCCCTCGGAAAAGCGCGGCACTGAGGTGCGCTTTCTGCCCG
ACACCGAGATCTTCAAGGAGAACAGCGACTTCCATTACGAGATCCTGGCCAAGCGCCTGCGCGAACTGTCCTTCCTGAAC
AACGGCGTGCGCATCCGCCTGATCGACGAGCGCACCGGCAAGGAAGATGACTTCTCGGGTGCCGGTGGCGTCAAGGGCTT
TGTGGAATTCATCAACGGCACCAAGAAGGTGCTGCACCCCACGGCCTTCCACGCCACGGGCGCGCGCCCGGCCGAGACCT
ACGGCGGCATCCCCGGCACCGAGATTGGCGTCGAAGTGGCCATGCAGTGGAACGACGGCTACAACGAGCAGGTCCTCTGT
TTCACCAACAACATCCCGCAGCGTGACGGCGGCACCCACCTGACCGGCCTGCGCGCGGCGATGACGCGCGTGATCGGCAA
GTACATCGAGCAGAACGAGCTGGCCAAGAAGGCCAAGGTCGAGGTCAGCGGCGACGACATGCGCGAAGGCCTGTGCTGCG
TCCTGAGCGTGAAGGTGCCCGAGCCCAAGTTCAGCAGCCAGACCAAGGACAAGCTGGTCAGCTCCGAAGTGCGCGCGCCG
GTCGAGGACATCGTCGCCAAGGCGCTCACCGACTATCTGGAAGAAAAACCGAACGACGCGAAGATCCTCTGCGGCAAGAT
CGTCGAGGCCGCGCGCGCCCGCGAGGCCGCGCGCAAGGCACGCGAGATGACACGCCGCAAAGGCGTGCTGGACGGCATGG
GCCTGCCCGGCAAGCTGGCCGACTGCCAGGAAAAAGACCCGGCGCTGTGCGAGATCTACATCGTCGAGGGCGACTCCGCC
GGCGGCTCCGCCAAGCAGGGGCGCGACCGCAAGTTCCAGGCCATCCTGCCGCTGCGCGGCAAGATCCTGAACGTGGAGAA
GGCGCGCTACGAGAAGCTGCTGTCCAGCAACGAAATCGTCACCCTCATCACCGCCCTGGGCACCGGCATCGGCAAGGCCG
CCGTGGAATCGGGCAAGAGCGGCGCTGACGACTTCGACGTGGCCAAGCTGCGTTACCACCGCATCATCATCATGACCGAC
GCGGACGTGGACGGCGCCCACATCCGCACGCTGCTGTTGACCTTCTTCTACCGCCAGATGCCCGAGCTGGTGGAGCGCGG
TCACATCTACATCGCCCAACCGCCGCTGTACAAGGTGAAGAACGGCAAGGAAGAGCTGTACCTGAAAGACGCCGCGGCGC
TGGACACCTTCCTGCTGCGCGTGGCGCTGAACCACGCCAGCATCACCACCAGCGGCGACCAGCCCCGCACGCTGGCCGGC
GACGATCTGGCCCAGCTGGCGCGCACGCACCAGCATGCCGAGCAGGTGATCGCGCGCCTGTCCGCCTTCATGGATGCGGA
GGCGCTGCGCGCCGTGGCCGACGGCGTGGTCTTCAACCTGGACACCCTGCAGGACGCCGAGGCCAGTGCCGTGCAGCTGC
AGGCCAAGCTGCGCGAGCTCAACACCAACGGCGTGCCCGCCGATGTGGCGGGCGAGATCGACCCGCGCACCGACAAGCCC
ATCCTGCGCATCAGCCGCCACCACCATGGCAACATCAAGAGCAGCCTGATCACACAGGACTTCGTGGCCGGCGACGACTA
CGCCGCCCTGGCCCAGGCCGCCGAGAGCTTCCGCGGGCTGATCCAGGAAGGTGCCAAGGTCACGCGTGGCGAGGGCGAGA
AGGCCAAGGAGGAAAAGGTGGGCGACTTCCGCCAGGCCATGGCCTGGCTGATCAGCGAAGCCGAGCGCACCACCAGCCGC
CAGCGCTACAAGGGCCTGGGCGAGATGAACCCCGAGCAGCTGTGGGAAACCACCATGGACCCCGAGGTGCGCCGCCTCTT
GCGCGTGCAGATCGACGACGCCATCGAGGCCGATCGCGTGTTCACCATGCTGATGGGCGACGAGGTTGAGCCCCGCCGCG
ACTTCATCGAGACCAATGCGCTGCGCGCGGGCAACATCGACGTCTGA

Upstream 100 bases:

>100_bases
AGCTGTTAGCGCTTGATGGACGGGCGCTAGCGGCACATTTGATTCCTATAAGGCCGCCGGCACGCCGTGCCGGTGCGCCT
GGACCGAAGGCAGCCCTTTC

Downstream 100 bases:

>100_bases
TCGTTGCTGTAAAGGGCCCATCCCCCGACGATTGGGGCGCCGTCAGTGAGAAAAAGCCATGGACCAACATCCATGGCTTT
TTCATCGCTGGAGACAAAAC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 868; Mature: 867

Protein sequence:

>868_residues
MTAEQSQPAPEQPISAPAEQPVASVAGSDGYGEGAIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDNSIDEALAG
HCDDIVVTIHTDNSISVVDNGRGIPTGVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKKL
RLTVRRDGQTHQLEFSKGVVQNRLIEIVDGFEVSPMKVIGPSEKRGTEVRFLPDTEIFKENSDFHYEILAKRLRELSFLN
NGVRIRLIDERTGKEDDFSGAGGVKGFVEFINGTKKVLHPTAFHATGARPAETYGGIPGTEIGVEVAMQWNDGYNEQVLC
FTNNIPQRDGGTHLTGLRAAMTRVIGKYIEQNELAKKAKVEVSGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSSEVRAP
VEDIVAKALTDYLEEKPNDAKILCGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDSA
GGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLSSNEIVTLITALGTGIGKAAVESGKSGADDFDVAKLRYHRIIIMTD
ADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKNGKEELYLKDAAALDTFLLRVALNHASITTSGDQPRTLAG
DDLAQLARTHQHAEQVIARLSAFMDAEALRAVADGVVFNLDTLQDAEASAVQLQAKLRELNTNGVPADVAGEIDPRTDKP
ILRISRHHHGNIKSSLITQDFVAGDDYAALAQAAESFRGLIQEGAKVTRGEGEKAKEEKVGDFRQAMAWLISEAERTTSR
QRYKGLGEMNPEQLWETTMDPEVRRLLRVQIDDAIEADRVFTMLMGDEVEPRRDFIETNALRAGNIDV

Sequences:

>Translated_868_residues
MTAEQSQPAPEQPISAPAEQPVASVAGSDGYGEGAIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDNSIDEALAG
HCDDIVVTIHTDNSISVVDNGRGIPTGVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKKL
RLTVRRDGQTHQLEFSKGVVQNRLIEIVDGFEVSPMKVIGPSEKRGTEVRFLPDTEIFKENSDFHYEILAKRLRELSFLN
NGVRIRLIDERTGKEDDFSGAGGVKGFVEFINGTKKVLHPTAFHATGARPAETYGGIPGTEIGVEVAMQWNDGYNEQVLC
FTNNIPQRDGGTHLTGLRAAMTRVIGKYIEQNELAKKAKVEVSGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSSEVRAP
VEDIVAKALTDYLEEKPNDAKILCGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDSA
GGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLSSNEIVTLITALGTGIGKAAVESGKSGADDFDVAKLRYHRIIIMTD
ADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKNGKEELYLKDAAALDTFLLRVALNHASITTSGDQPRTLAG
DDLAQLARTHQHAEQVIARLSAFMDAEALRAVADGVVFNLDTLQDAEASAVQLQAKLRELNTNGVPADVAGEIDPRTDKP
ILRISRHHHGNIKSSLITQDFVAGDDYAALAQAAESFRGLIQEGAKVTRGEGEKAKEEKVGDFRQAMAWLISEAERTTSR
QRYKGLGEMNPEQLWETTMDPEVRRLLRVQIDDAIEADRVFTMLMGDEVEPRRDFIETNALRAGNIDV
>Mature_867_residues
TAEQSQPAPEQPISAPAEQPVASVAGSDGYGEGAIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDNSIDEALAGH
CDDIVVTIHTDNSISVVDNGRGIPTGVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKKLR
LTVRRDGQTHQLEFSKGVVQNRLIEIVDGFEVSPMKVIGPSEKRGTEVRFLPDTEIFKENSDFHYEILAKRLRELSFLNN
GVRIRLIDERTGKEDDFSGAGGVKGFVEFINGTKKVLHPTAFHATGARPAETYGGIPGTEIGVEVAMQWNDGYNEQVLCF
TNNIPQRDGGTHLTGLRAAMTRVIGKYIEQNELAKKAKVEVSGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSSEVRAPV
EDIVAKALTDYLEEKPNDAKILCGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDSAG
GSAKQGRDRKFQAILPLRGKILNVEKARYEKLLSSNEIVTLITALGTGIGKAAVESGKSGADDFDVAKLRYHRIIIMTDA
DVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKNGKEELYLKDAAALDTFLLRVALNHASITTSGDQPRTLAGD
DLAQLARTHQHAEQVIARLSAFMDAEALRAVADGVVFNLDTLQDAEASAVQLQAKLRELNTNGVPADVAGEIDPRTDKPI
LRISRHHHGNIKSSLITQDFVAGDDYAALAQAAESFRGLIQEGAKVTRGEGEKAKEEKVGDFRQAMAWLISEAERTTSRQ
RYKGLGEMNPEQLWETTMDPEVRRLLRVQIDDAIEADRVFTMLMGDEVEPRRDFIETNALRAGNIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=596, Percent_Identity=25.503355704698, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI19913406, Length=614, Percent_Identity=24.9185667752443, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI48994957, Length=846, Percent_Identity=56.5011820330969, Blast_Score=914, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=582, Percent_Identity=38.4879725085911, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535065, Length=614, Percent_Identity=24.7557003257329, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=543, Percent_Identity=24.4935543278085, Blast_Score=102, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI212645657, Length=161, Percent_Identity=33.5403726708075, Blast_Score=76, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=619, Percent_Identity=25.3634894991922, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI17136538, Length=581, Percent_Identity=23.5800344234079, Blast_Score=116, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 94998; Mature: 94867

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEQSQPAPEQPISAPAEQPVASVAGSDGYGEGAIQILEGLEAVRKRPGMYIGDTSDGT
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCC
GLHHLVFEVVDNSIDEALAGHCDDIVVTIHTDNSISVVDNGRGIPTGVKMDDKHEPKRSA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHH
AEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKKLRLTVRRDGQTHQLEFSKGVV
HHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHEEEEECCCCCEEEEHHHHHH
QNRLIEIVDGFEVSPMKVIGPSEKRGTEVRFLPDTEIFKENSDFHYEILAKRLRELSFLN
HHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHC
NGVRIRLIDERTGKEDDFSGAGGVKGFVEFINGTKKVLHPTAFHATGARPAETYGGIPGT
CCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCHHCCCCCCCHHHCCCCCCC
EIGVEVAMQWNDGYNEQVLCFTNNIPQRDGGTHLTGLRAAMTRVIGKYIEQNELAKKAKV
CCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
EVSGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEDIVAKALTDYLEEKPNDA
EECCCHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCH
KILCGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDSA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCC
GGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLSSNEIVTLITALGTGIGKAAVESGKS
CCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHCCCC
GADDFDVAKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVK
CCCCHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEECC
NGKEELYLKDAAALDTFLLRVALNHASITTSGDQPRTLAGDDLAQLARTHQHAEQVIARL
CCCHHEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SAFMDAEALRAVADGVVFNLDTLQDAEASAVQLQAKLRELNTNGVPADVAGEIDPRTDKP
HHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ILRISRHHHGNIKSSLITQDFVAGDDYAALAQAAESFRGLIQEGAKVTRGEGEKAKEEKV
HHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
GDFRQAMAWLISEAERTTSRQRYKGLGEMNPEQLWETTMDPEVRRLLRVQIDDAIEADRV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FTMLMGDEVEPRRDFIETNALRAGNIDV
HHHHHCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TAEQSQPAPEQPISAPAEQPVASVAGSDGYGEGAIQILEGLEAVRKRPGMYIGDTSDGT
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCC
GLHHLVFEVVDNSIDEALAGHCDDIVVTIHTDNSISVVDNGRGIPTGVKMDDKHEPKRSA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHH
AEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKKLRLTVRRDGQTHQLEFSKGVV
HHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHEEEEECCCCCEEEEHHHHHH
QNRLIEIVDGFEVSPMKVIGPSEKRGTEVRFLPDTEIFKENSDFHYEILAKRLRELSFLN
HHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHC
NGVRIRLIDERTGKEDDFSGAGGVKGFVEFINGTKKVLHPTAFHATGARPAETYGGIPGT
CCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCHHCCCCCCCHHHCCCCCCC
EIGVEVAMQWNDGYNEQVLCFTNNIPQRDGGTHLTGLRAAMTRVIGKYIEQNELAKKAKV
CCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
EVSGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEDIVAKALTDYLEEKPNDA
EECCCHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCH
KILCGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDSA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCC
GGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLSSNEIVTLITALGTGIGKAAVESGKS
CCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHCCCC
GADDFDVAKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVK
CCCCHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEECC
NGKEELYLKDAAALDTFLLRVALNHASITTSGDQPRTLAGDDLAQLARTHQHAEQVIARL
CCCHHEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SAFMDAEALRAVADGVVFNLDTLQDAEASAVQLQAKLRELNTNGVPADVAGEIDPRTDKP
HHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ILRISRHHHGNIKSSLITQDFVAGDDYAALAQAAESFRGLIQEGAKVTRGEGEKAKEEKV
HHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
GDFRQAMAWLISEAERTTSRQRYKGLGEMNPEQLWETTMDPEVRRLLRVQIDDAIEADRV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FTMLMGDEVEPRRDFIETNALRAGNIDV
HHHHHCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]