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The map label for this gene is yoaE [H]

Identifier: 222102891

GI number: 222102891

Start: 568363

End: 570489

Strand: Reverse

Name: yoaE [H]

Synonym: Avi_7633

Alternate gene names: 222102891

Gene position: 570489-568363 (Counterclockwise)

Preceding gene: 222102894

Following gene: 222102890

Centisome position: 90.3

GC content: 59.14

Gene sequence:

>2127_bases
ATGGCCCGTTCTGTTGTTGTAGGTGCATGTCCGCATGACTGTCCTGATACCTGCTCGATCCTGACGACCGTCGAGGACGG
CAAGGCGCTTGCTGTGCGGGGCAATCCAGACCATCCCTTCACCCGCGGCCGGCTCTGCGTGAAGGTCAACAATTACCAGG
ATCGGGTCTATAGCGATAAGCGCCTGCTTTACCCGATGCGGCGCGTCGGCCCTAAAGGTAGCGGCCAGTTCGTTCGAATC
TCTTGGGATGACGCGCTCGAAGAGATCGCCATCCGCTGGAAGGATATCATCGCGTCCCACGGCGCTCAGGCGATATTGCC
CTACAGCTATCTTGGCACGCAGGGTATTCTCAATGGCCTCAATGTCGGCGATCCTCTGTTCAACAAGCTGGGCGCCTCGG
TGAGCGAGCGCACCTTCTGTGATTCAGGCTCATGCACGGCCTATATGATGACGATTGGGCATACACCGGGTGTCGATCCC
GAGAGCTTCGTTCATTCCAAATATATCATTCTCTGGGCCTGCAACACGCTCAGTACCAATTCGCATCATTGGCCCTTCAT
TGAGGAAGCCCGAAAGAGCGGTGCTAAACTTGTGGTGATCGATCCGGTGCGTACCCGCACTGCGCGGCTTGCCGACTGGC
ACATTCCGATCCGGCCTGGCACGGATGGGGCACTGGCCATGGCCATGATGCACGTCATCATCACGGAAAATCTGGTGGAT
CGGGACTATGTCGACAAGCACACGCTGGGATATGACGAACTGGTTGAGCGGGTGGCGGAATACACACCGGAATTCGCCTC
TTTGGAAACCGGCATTCCCGTTGATGACATCCTGAAACTGGCTCGTGAATACGCGACCACGCCTGCGGCGGTCGTGCGGA
TCGGGGTGGCGGTGGAACGTCATGCTGGCGGTGGCCAGACGGTTCGTGCCATTGCCTGCCTGCCAGCGTTGATCGGTGCA
TGGAAACATGTCGGCGGAGGGCTTCTGCAATTGCCGATCTGGGCCTTCCCGGTAAACTGGGGCGGGCTGATGCGTCCCGA
TCTCCAGCCTGAGAAGATGCGGGTGATCAATTCCTGGCGCCTTGGCCAGGCGCTGACGGGCGCTCTTGAACTGGACCCTC
CGATCCGTGCCTTGTTCGTATACAATGCCAATCCCATGGCCATGGTCACCGAGCAGGAAAAGCTGGAACAGGGGCTGGGG
CGGGAAGATCTATTCACCGTCGTCAGCGAGCATTTCATCACCGACACCGCCAGATATGCTGATATCCTTTTGCCAGCGAC
CACGCAGCTGGAACAGAAGGACATCATGTTCTCCTGGGGCCATCTCTATCTGTCCTACAACAATCCAGCGATCGAACCGC
TCGGCGAGGCGGTTTCAAACACGGAATTGTTCCGGCGCCTTGCCGGCGCACTGGGCATCGACGATCCCTTCTTCTTCCGC
TCGGACGACGAGATGATCGAAGCATCGATGGATTGGGCAAGCCCCGTTCTTGAAGGGATCACGCTCGATCAATTGAAACA
GTCGGGATATATGCGATTGAACATGCCGGCTGCCGACCGATGGGCGCCGCATCGTGACGGAAATTTTCCGACACCTACGG
GCAAATGCGAATTCAAATCAACCCTCGCCGAAAGCGGCAATTTCGTCGCCCCGCTGTTCAGGCAGGGTTACGGTGGCGAC
CAATCGGGCGAAGCGGTCGATCCGCTCCCGCATTACATCCGTCCCAACGAGAGCCCCGCAACCCATCCGGTTCTGGCGCA
GCAGTATCCGCTCAGCCTGATTTCTCCGAAAAGCCACGCCTTTCTCAACTCGAATTACGGGAATCTGCCAGCGCAGATTG
CGCAGGCCGGCGAGGAACAGGCGGTGCTGCTGCACCCTGACGACGCCAGCGAACGCGGTATCATCGCCGGAGCGCCGATC
CGCGTGTTCAATGATCGCGGAATGTTCGAGGCATTCGCGACATTGTCGCCCGATGTCATGCGTGGCGTTGTGGTTGCTCC
GTCCGGCTACTGGCAGCGCTCCAACCGAAAGGGCGCGACCGTGCATGCCGTGACGCCGCCCGCCTATGCCGATCTCGGCC
GGGCGCCGACATTTTCCGATGCGCTGGTCCAGGTTGCAATGGCCTGA

Upstream 100 bases:

>100_bases
TCGCAGGAATGGAACAGTAAAAAGCAGTTTTGGATAACGCCGAGGGCTGAATTTTACAGCAGAATGCCGCCATTCGAAGC
GTGTAAGCGAGGTAAAAGCG

Downstream 100 bases:

>100_bases
CTCCCTCAAGATTTTCCGGGCGGATGATGGCGTGTTCACCAAATCGTCTGCCACATATGATGAAAGCTGACCATGACCTA
TGTTGTAACCGACCAATGCT

Product: reductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MARSVVVGACPHDCPDTCSILTTVEDGKALAVRGNPDHPFTRGRLCVKVNNYQDRVYSDKRLLYPMRRVGPKGSGQFVRI
SWDDALEEIAIRWKDIIASHGAQAILPYSYLGTQGILNGLNVGDPLFNKLGASVSERTFCDSGSCTAYMMTIGHTPGVDP
ESFVHSKYIILWACNTLSTNSHHWPFIEEARKSGAKLVVIDPVRTRTARLADWHIPIRPGTDGALAMAMMHVIITENLVD
RDYVDKHTLGYDELVERVAEYTPEFASLETGIPVDDILKLAREYATTPAAVVRIGVAVERHAGGGQTVRAIACLPALIGA
WKHVGGGLLQLPIWAFPVNWGGLMRPDLQPEKMRVINSWRLGQALTGALELDPPIRALFVYNANPMAMVTEQEKLEQGLG
REDLFTVVSEHFITDTARYADILLPATTQLEQKDIMFSWGHLYLSYNNPAIEPLGEAVSNTELFRRLAGALGIDDPFFFR
SDDEMIEASMDWASPVLEGITLDQLKQSGYMRLNMPAADRWAPHRDGNFPTPTGKCEFKSTLAESGNFVAPLFRQGYGGD
QSGEAVDPLPHYIRPNESPATHPVLAQQYPLSLISPKSHAFLNSNYGNLPAQIAQAGEEQAVLLHPDDASERGIIAGAPI
RVFNDRGMFEAFATLSPDVMRGVVVAPSGYWQRSNRKGATVHAVTPPAYADLGRAPTFSDALVQVAMA

Sequences:

>Translated_708_residues
MARSVVVGACPHDCPDTCSILTTVEDGKALAVRGNPDHPFTRGRLCVKVNNYQDRVYSDKRLLYPMRRVGPKGSGQFVRI
SWDDALEEIAIRWKDIIASHGAQAILPYSYLGTQGILNGLNVGDPLFNKLGASVSERTFCDSGSCTAYMMTIGHTPGVDP
ESFVHSKYIILWACNTLSTNSHHWPFIEEARKSGAKLVVIDPVRTRTARLADWHIPIRPGTDGALAMAMMHVIITENLVD
RDYVDKHTLGYDELVERVAEYTPEFASLETGIPVDDILKLAREYATTPAAVVRIGVAVERHAGGGQTVRAIACLPALIGA
WKHVGGGLLQLPIWAFPVNWGGLMRPDLQPEKMRVINSWRLGQALTGALELDPPIRALFVYNANPMAMVTEQEKLEQGLG
REDLFTVVSEHFITDTARYADILLPATTQLEQKDIMFSWGHLYLSYNNPAIEPLGEAVSNTELFRRLAGALGIDDPFFFR
SDDEMIEASMDWASPVLEGITLDQLKQSGYMRLNMPAADRWAPHRDGNFPTPTGKCEFKSTLAESGNFVAPLFRQGYGGD
QSGEAVDPLPHYIRPNESPATHPVLAQQYPLSLISPKSHAFLNSNYGNLPAQIAQAGEEQAVLLHPDDASERGIIAGAPI
RVFNDRGMFEAFATLSPDVMRGVVVAPSGYWQRSNRKGATVHAVTPPAYADLGRAPTFSDALVQVAMA
>Mature_707_residues
ARSVVVGACPHDCPDTCSILTTVEDGKALAVRGNPDHPFTRGRLCVKVNNYQDRVYSDKRLLYPMRRVGPKGSGQFVRIS
WDDALEEIAIRWKDIIASHGAQAILPYSYLGTQGILNGLNVGDPLFNKLGASVSERTFCDSGSCTAYMMTIGHTPGVDPE
SFVHSKYIILWACNTLSTNSHHWPFIEEARKSGAKLVVIDPVRTRTARLADWHIPIRPGTDGALAMAMMHVIITENLVDR
DYVDKHTLGYDELVERVAEYTPEFASLETGIPVDDILKLAREYATTPAAVVRIGVAVERHAGGGQTVRAIACLPALIGAW
KHVGGGLLQLPIWAFPVNWGGLMRPDLQPEKMRVINSWRLGQALTGALELDPPIRALFVYNANPMAMVTEQEKLEQGLGR
EDLFTVVSEHFITDTARYADILLPATTQLEQKDIMFSWGHLYLSYNNPAIEPLGEAVSNTELFRRLAGALGIDDPFFFRS
DDEMIEASMDWASPVLEGITLDQLKQSGYMRLNMPAADRWAPHRDGNFPTPTGKCEFKSTLAESGNFVAPLFRQGYGGDQ
SGEAVDPLPHYIRPNESPATHPVLAQQYPLSLISPKSHAFLNSNYGNLPAQIAQAGEEQAVLLHPDDASERGIIAGAPIR
VFNDRGMFEAFATLSPDVMRGVVVAPSGYWQRSNRKGATVHAVTPPAYADLGRAPTFSDALVQVAMA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=784, Percent_Identity=30.2295918367347, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1787870, Length=759, Percent_Identity=30.0395256916996, Blast_Score=245, Evalue=7e-66,
Organism=Escherichia coli, GI171474008, Length=740, Percent_Identity=29.7297297297297, Blast_Score=232, Evalue=6e-62,
Organism=Escherichia coli, GI3868721, Length=703, Percent_Identity=23.7553342816501, Blast_Score=146, Evalue=6e-36,
Organism=Escherichia coli, GI1787231, Length=758, Percent_Identity=24.934036939314, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI87081994, Length=700, Percent_Identity=23.8571428571429, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI145693196, Length=737, Percent_Identity=24.966078697422, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788534, Length=361, Percent_Identity=22.7146814404432, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787741, Length=289, Percent_Identity=25.9515570934256, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787477, Length=286, Percent_Identity=26.5734265734266, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 77711; Mature: 77580

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSVVVGACPHDCPDTCSILTTVEDGKALAVRGNPDHPFTRGRLCVKVNNYQDRVYSDK
CCCCEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHCCC
RLLYPMRRVGPKGSGQFVRISWDDALEEIAIRWKDIIASHGAQAILPYSYLGTQGILNGL
HHCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCHHHHHCCC
NVGDPLFNKLGASVSERTFCDSGSCTAYMMTIGHTPGVDPESFVHSKYIILWACNTLSTN
CCCCHHHHHHCCCHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCEEEEEEECCCCCC
SHHWPFIEEARKSGAKLVVIDPVRTRTARLADWHIPIRPGTDGALAMAMMHVIITENLVD
CCCCCHHHHHHHCCCEEEEECCCCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHC
RDYVDKHTLGYDELVERVAEYTPEFASLETGIPVDDILKLAREYATTPAAVVRIGVAVER
CCCCCHHCCCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHHEEEEEEEE
HAGGGQTVRAIACLPALIGAWKHVGGGLLQLPIWAFPVNWGGLMRPDLQPEKMRVINSWR
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCCCCCHHHHHHHHHHH
LGQALTGALELDPPIRALFVYNANPMAMVTEQEKLEQGLGREDLFTVVSEHFITDTARYA
CCHHHCCCEECCCCEEEEEEECCCCEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DILLPATTQLEQKDIMFSWGHLYLSYNNPAIEPLGEAVSNTELFRRLAGALGIDDPFFFR
HEEECCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEC
SDDEMIEASMDWASPVLEGITLDQLKQSGYMRLNMPAADRWAPHRDGNFPTPTGKCEFKS
CCHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
TLAESGNFVAPLFRQGYGGDQSGEAVDPLPHYIRPNESPATHPVLAQQYPLSLISPKSHA
HHHHCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCE
FLNSNYGNLPAQIAQAGEEQAVLLHPDDASERGIIAGAPIRVFNDRGMFEAFATLSPDVM
EECCCCCCCCHHHHHCCCCCEEEECCCCCCCCCEEECCCEEEECCCCHHHHHHHCCHHHH
RGVVVAPSGYWQRSNRKGATVHAVTPPAYADLGRAPTFSDALVQVAMA
CCEEECCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
ARSVVVGACPHDCPDTCSILTTVEDGKALAVRGNPDHPFTRGRLCVKVNNYQDRVYSDK
CCCEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHCCC
RLLYPMRRVGPKGSGQFVRISWDDALEEIAIRWKDIIASHGAQAILPYSYLGTQGILNGL
HHCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCHHHHHCCC
NVGDPLFNKLGASVSERTFCDSGSCTAYMMTIGHTPGVDPESFVHSKYIILWACNTLSTN
CCCCHHHHHHCCCHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCEEEEEEECCCCCC
SHHWPFIEEARKSGAKLVVIDPVRTRTARLADWHIPIRPGTDGALAMAMMHVIITENLVD
CCCCCHHHHHHHCCCEEEEECCCCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHC
RDYVDKHTLGYDELVERVAEYTPEFASLETGIPVDDILKLAREYATTPAAVVRIGVAVER
CCCCCHHCCCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHHEEEEEEEE
HAGGGQTVRAIACLPALIGAWKHVGGGLLQLPIWAFPVNWGGLMRPDLQPEKMRVINSWR
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCCCCCHHHHHHHHHHH
LGQALTGALELDPPIRALFVYNANPMAMVTEQEKLEQGLGREDLFTVVSEHFITDTARYA
CCHHHCCCEECCCCEEEEEEECCCCEEEEECHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DILLPATTQLEQKDIMFSWGHLYLSYNNPAIEPLGEAVSNTELFRRLAGALGIDDPFFFR
HEEECCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEC
SDDEMIEASMDWASPVLEGITLDQLKQSGYMRLNMPAADRWAPHRDGNFPTPTGKCEFKS
CCHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHH
TLAESGNFVAPLFRQGYGGDQSGEAVDPLPHYIRPNESPATHPVLAQQYPLSLISPKSHA
HHHHCCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCE
FLNSNYGNLPAQIAQAGEEQAVLLHPDDASERGIIAGAPIRVFNDRGMFEAFATLSPDVM
EECCCCCCCCHHHHHCCCCCEEEECCCCCCCCCEEECCCEEEECCCCHHHHHHHCCHHHH
RGVVVAPSGYWQRSNRKGATVHAVTPPAYADLGRAPTFSDALVQVAMA
CCEEECCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]