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The map label for this gene is fnrN [H]

Identifier: 222102881

GI number: 222102881

Start: 559070

End: 559768

Strand: Direct

Name: fnrN [H]

Synonym: Avi_7621

Alternate gene names: 222102881

Gene position: 559070-559768 (Clockwise)

Preceding gene: 222102880

Following gene: 222102882

Centisome position: 88.49

GC content: 54.22

Gene sequence:

>699_bases
TTGGGTGAAGTATCGCCCGTATTCCCAATCGATTTTGTGATATCCGCCGAAATCGGCAGTAAATCTCAGATGTGCAAGCT
CGTTGCGCCGCGCACCAGAGTTTTCCTGCATGATTTCCCAAGATCGAGCGAATTTCTACTCCAGGACGGGTGCATCGCAC
TCTATCAATCTCTGTACGACGGTCGCAGACAAATACTTGACATCCTTGGGCCGGGCTCCTTTCTCGGACTATCGATTGGC
AGCTCTGTCAATTGCTGTGCCGAAACCTTGGCGTTTACGCAGATTCAACCGGTTGCGCAGCTCACCGATCACGCTCTCGG
CAGTGCGCTCAAACATATGCTGCGCCGGTCGCAAGCATTAGTGACCTTGCTCGGCCGAAAAACGGCCCAGGAAAAAGTTG
CGTTTGCCCTCCTCGATCTCGCTGAGAGGTTTACCCTGACGTCGAAGGCTTCGCAGCTGCGGAAAACGACATTCAGGCTT
TTCCTGACCAGAGCCGATCTTGCCGATTGGTTAGGACTGACACTTGAGACCGTCAGTCGTTGCCTCAACAGCTTCAAGCG
CAGCGGTATACTCACCTTCAACCATCCCGAAATCATCACCATAGCCGACGAGAACGCCTTGCGATGCCTCGCCTCAGGCC
AGCCTTCGCTTGCCGGGCAGAAATCCGCAAAGGGCGATGTCTCTCTTCAACCGTCATAG

Upstream 100 bases:

>100_bases
TTCACAGCAGCCCTTCAGTCAGCGCCGGTTGAGCGTTCCAAACTCTGGGACGTGACGACCGGTGCAGCGCCGGACACACG
TCATGTTCAACAGGACAGCA

Downstream 100 bases:

>100_bases
CACTGGGGGACGGTTATCATGCTCGTCGTCTTGCTTCTTTTTTCGCTGACCGGCGCCGTGCTGGTGATGGTGATGGTGAT
CGCATTCGACCATCTCATCA

Product: transcriptional activator Crp family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MGEVSPVFPIDFVISAEIGSKSQMCKLVAPRTRVFLHDFPRSSEFLLQDGCIALYQSLYDGRRQILDILGPGSFLGLSIG
SSVNCCAETLAFTQIQPVAQLTDHALGSALKHMLRRSQALVTLLGRKTAQEKVAFALLDLAERFTLTSKASQLRKTTFRL
FLTRADLADWLGLTLETVSRCLNSFKRSGILTFNHPEIITIADENALRCLASGQPSLAGQKSAKGDVSLQPS

Sequences:

>Translated_232_residues
MGEVSPVFPIDFVISAEIGSKSQMCKLVAPRTRVFLHDFPRSSEFLLQDGCIALYQSLYDGRRQILDILGPGSFLGLSIG
SSVNCCAETLAFTQIQPVAQLTDHALGSALKHMLRRSQALVTLLGRKTAQEKVAFALLDLAERFTLTSKASQLRKTTFRL
FLTRADLADWLGLTLETVSRCLNSFKRSGILTFNHPEIITIADENALRCLASGQPSLAGQKSAKGDVSLQPS
>Mature_231_residues
GEVSPVFPIDFVISAEIGSKSQMCKLVAPRTRVFLHDFPRSSEFLLQDGCIALYQSLYDGRRQILDILGPGSFLGLSIGS
SVNCCAETLAFTQIQPVAQLTDHALGSALKHMLRRSQALVTLLGRKTAQEKVAFALLDLAERFTLTSKASQLRKTTFRLF
LTRADLADWLGLTLETVSRCLNSFKRSGILTFNHPEIITIADENALRCLASGQPSLAGQKSAKGDVSLQPS

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=105, Percent_Identity=35.2380952380952, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25331; Mature: 25199

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVSPVFPIDFVISAEIGSKSQMCKLVAPRTRVFLHDFPRSSEFLLQDGCIALYQSLYD
CCCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHH
GRRQILDILGPGSFLGLSIGSSVNCCAETLAFTQIQPVAQLTDHALGSALKHMLRRSQAL
HHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTLLGRKTAQEKVAFALLDLAERFTLTSKASQLRKTTFRLFLTRADLADWLGLTLETVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLNSFKRSGILTFNHPEIITIADENALRCLASGQPSLAGQKSAKGDVSLQPS
HHHHHHHCCEEEECCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCEECCCC
>Mature Secondary Structure 
GEVSPVFPIDFVISAEIGSKSQMCKLVAPRTRVFLHDFPRSSEFLLQDGCIALYQSLYD
CCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHH
GRRQILDILGPGSFLGLSIGSSVNCCAETLAFTQIQPVAQLTDHALGSALKHMLRRSQAL
HHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTLLGRKTAQEKVAFALLDLAERFTLTSKASQLRKTTFRLFLTRADLADWLGLTLETVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLNSFKRSGILTFNHPEIITIADENALRCLASGQPSLAGQKSAKGDVSLQPS
HHHHHHHCCEEEECCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2663474; 11481432 [H]