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The map label for this gene is asg [H]

Identifier: 222102658

GI number: 222102658

Start: 298934

End: 299899

Strand: Reverse

Name: asg [H]

Synonym: Avi_7322

Alternate gene names: 222102658

Gene position: 299899-298934 (Counterclockwise)

Preceding gene: 222102659

Following gene: 222102656

Centisome position: 47.47

GC content: 60.35

Gene sequence:

>966_bases
ATGAGCAATACCCAACCAAGCCCCTTTGCGATTGCCATTCACGGCGGCTGCGGCGTGATGAACAAGGCCGACATGCCGGA
GAGCGAATGGGAAGCCGCCCGCGCTGACCTCAAACGCGCATTAAAGGCCGGATGGAGCTTGTTGCAACAAGGCGCATCCG
CGCTCGACGCCGTCGAAGCCGCCGTTATGGTGATGGAGGATTCGGAGCATTTCAACGCGGGCCATGGCGCAGCACTCAAC
ACCGATGGCGAACACGAGTTGGATGCCTCCATTATGGATGGTCAGACCCTTGAGGCGGGTGCCATTGCCATGGCACGCAC
GATCCGCAACCCGATTCGTGCCGCCCGCAAACTGATGCACAGCGGCGAGACCGTGATGCTGGGGGCAAGTGCTGCCGATG
CCTTTGCCCAAAAATCGGGCCTGCCGATGGTGGAACAGTCCTATTTCACCACCGAGCGCCGGGTTAAAGCACTGGCATCG
TTGAAAGAACACGCCAAAGCCGGAACGGCAGCCCTTGCCAGCGAGGCCGAAAAGCATGGCACGGTTGGCGCCGTGGCGCT
GGACAGCCACGGCCACCTTGCCGCCGCCACCTCCACTGGCGGCTTCAACAACAAGCCTTCAGGCCGCATTGGCGACACCG
CTGTGATCGGCGCCGGAACCTACGCCCGCGATGGCGTGGTGGCTGTCTCCGGTACTGGCAAGGGTGAGTTTTTCATCCGC
CATGCCGTGGGCCACGAAATCGCCAGCCGCATGCGGTATTTGGGCGAAAATCTGGAAGACGCCTGCCAGCATGTCGTGTT
CACCGATCTTGCCCCCCATCATATCGGAGCAGGGCTTGTCGCGGTTGGTGCAGATGGCTCCATCACAGCACCTTATAATA
CCGCCGGGATGTTTCGCGGCTGGATTAATCTGGATGGGCACATGGTTGTCGCAAGCCATGACACGCTGTTTGAAATGCAT
ATCTAG

Upstream 100 bases:

>100_bases
AAGCGTTGAACATGCCGTGCGGATGATCAACAGCCTATCCGCCATGTCCACCATGCTGCGCTAACGGCGGGCGATCTATT
TTGAGCATTTCGAGGCATTT

Downstream 100 bases:

>100_bases
AGAATTGTTTCGTTTTATGGAAACACTGAATGTTGGAGCAGTGTCGGAGGTTGACCTCTATGATCACTCCCGTAGAGCCG
TGATTTTTTGAGATAAAATG

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSNTQPSPFAIAIHGGCGVMNKADMPESEWEAARADLKRALKAGWSLLQQGASALDAVEAAVMVMEDSEHFNAGHGAALN
TDGEHELDASIMDGQTLEAGAIAMARTIRNPIRAARKLMHSGETVMLGASAADAFAQKSGLPMVEQSYFTTERRVKALAS
LKEHAKAGTAALASEAEKHGTVGAVALDSHGHLAAATSTGGFNNKPSGRIGDTAVIGAGTYARDGVVAVSGTGKGEFFIR
HAVGHEIASRMRYLGENLEDACQHVVFTDLAPHHIGAGLVAVGADGSITAPYNTAGMFRGWINLDGHMVVASHDTLFEMH
I

Sequences:

>Translated_321_residues
MSNTQPSPFAIAIHGGCGVMNKADMPESEWEAARADLKRALKAGWSLLQQGASALDAVEAAVMVMEDSEHFNAGHGAALN
TDGEHELDASIMDGQTLEAGAIAMARTIRNPIRAARKLMHSGETVMLGASAADAFAQKSGLPMVEQSYFTTERRVKALAS
LKEHAKAGTAALASEAEKHGTVGAVALDSHGHLAAATSTGGFNNKPSGRIGDTAVIGAGTYARDGVVAVSGTGKGEFFIR
HAVGHEIASRMRYLGENLEDACQHVVFTDLAPHHIGAGLVAVGADGSITAPYNTAGMFRGWINLDGHMVVASHDTLFEMH
I
>Mature_320_residues
SNTQPSPFAIAIHGGCGVMNKADMPESEWEAARADLKRALKAGWSLLQQGASALDAVEAAVMVMEDSEHFNAGHGAALNT
DGEHELDASIMDGQTLEAGAIAMARTIRNPIRAARKLMHSGETVMLGASAADAFAQKSGLPMVEQSYFTTERRVKALASL
KEHAKAGTAALASEAEKHGTVGAVALDSHGHLAAATSTGGFNNKPSGRIGDTAVIGAGTYARDGVVAVSGTGKGEFFIRH
AVGHEIASRMRYLGENLEDACQHVVFTDLAPHHIGAGLVAVGADGSITAPYNTAGMFRGWINLDGHMVVASHDTLFEMHI

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=235, Percent_Identity=42.5531914893617, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI145275200, Length=235, Percent_Identity=42.5531914893617, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI98991769, Length=269, Percent_Identity=33.8289962825279, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI285002251, Length=293, Percent_Identity=31.740614334471, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI285002253, Length=293, Percent_Identity=30.3754266211604, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1787050, Length=294, Percent_Identity=44.2176870748299, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI32564649, Length=272, Percent_Identity=27.5735294117647, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18921183, Length=321, Percent_Identity=35.202492211838, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=30.9701492537313, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33492; Mature: 33361

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTQPSPFAIAIHGGCGVMNKADMPESEWEAARADLKRALKAGWSLLQQGASALDAVEA
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVMVMEDSEHFNAGHGAALNTDGEHELDASIMDGQTLEAGAIAMARTIRNPIRAARKLMH
HEEEEECCCCCCCCCCCEECCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SGETVMLGASAADAFAQKSGLPMVEQSYFTTERRVKALASLKEHAKAGTAALASEAEKHG
CCCEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TVGAVALDSHGHLAAATSTGGFNNKPSGRIGDTAVIGAGTYARDGVVAVSGTGKGEFFIR
CEEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCEEEE
HAVGHEIASRMRYLGENLEDACQHVVFTDLAPHHIGAGLVAVGADGSITAPYNTAGMFRG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCEEEECCCCCEECCCCCHHHEEE
WINLDGHMVVASHDTLFEMHI
EEECCCEEEEEECCCEEEECC
>Mature Secondary Structure 
SNTQPSPFAIAIHGGCGVMNKADMPESEWEAARADLKRALKAGWSLLQQGASALDAVEA
CCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVMVMEDSEHFNAGHGAALNTDGEHELDASIMDGQTLEAGAIAMARTIRNPIRAARKLMH
HEEEEECCCCCCCCCCCEECCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SGETVMLGASAADAFAQKSGLPMVEQSYFTTERRVKALASLKEHAKAGTAALASEAEKHG
CCCEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TVGAVALDSHGHLAAATSTGGFNNKPSGRIGDTAVIGAGTYARDGVVAVSGTGKGEFFIR
CEEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCEEEE
HAVGHEIASRMRYLGENLEDACQHVVFTDLAPHHIGAGLVAVGADGSITAPYNTAGMFRG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCEEEECCCCCEECCCCCHHHEEE
WINLDGHMVVASHDTLFEMHI
EEECCCEEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]