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The map label for this gene is mcyB [H]

Identifier: 222102641

GI number: 222102641

Start: 254029

End: 255603

Strand: Reverse

Name: mcyB [H]

Synonym: Avi_7296

Alternate gene names: 222102641

Gene position: 255603-254029 (Counterclockwise)

Preceding gene: 222102642

Following gene: 222102640

Centisome position: 40.46

GC content: 61.52

Gene sequence:

>1575_bases
ATGCCGCAAACGGCGCTGCGCATCACCGGAGCCGTCGATGCGGAGGCTCTGAAGGCGGCATGGCAAGGCGCAATTGACCG
CCATCCGATCCTGCGTTCGGGGTTCTTCTGGGAGGAGCGCGACGAGCCGTTCCAGATCGCCTTCCGCAGCCTTCCGGTGG
CATTCACTACGCTGGACTGGACTGGTAACGATCCGGCAAGCGAGCGTGAACGGCTGCAAGCACTGTTTTCTGCCAACCGG
GCCGAGCCGTTTGACCTGCGCCGTCCGCCTCTGATCCGGGTCCAATGGATCAGCACCGGACAAGACCAGTCGATCATGGT
TGTCTGCTACCAACATATCATCCTCGACGGCTGGTCGATCCGTCAATTGCTCGACGAAGTGCTCTCGCTCTATCGGCGCG
CGGCAGGCAAGGCCGAGCCAGCCCTTCCGCCCGCCCGCCCTTACGGCGATTACATCGGTTGGTTGAAGAAGCGCGAGCGG
GCCGTGTCGCTTCGCTTCTGGAAGGATCGGCTTCAGGGTTTTGCGGGCACAACCCGGCTTCTGGCAGGGGACGCGACAGG
CCAGTTCGAGCGGCAATGCCGGGAAGTCCCAAAGCCGCTGCATGAGGCCGTGCTGGCCTATGGTGCCGCCCATGGGCTGA
CGCTCAACACGCTGCTGCAAGGCGCGTTGTCCTTGCTGATCGCGCGCCAGACCGGCAGCCGTGATCTCATTTTCGGCACA
ACCACAGCCGGGCGCCCCGTGACACTGGAGGGCGCAACGACGATGATCGGCCTCTTCATCAATACGCTGCCCGTGCGTGT
CCGCCTTAATGAGGGCCTATCCGTCTCGGACTGGCTCGCAACCATTCAGAAAGCCCATGCCGAAACGGGCGAACACGACC
ATGTACCCCTCGCCGCTATCCAAGGCCCGGGCGTTTCGCTTTTCGACACGCTGCTCGTCGTTGAGAATTTCCCAGTGCAG
GCGAAGCGAGCGGATGCGCCTGCACTGCTCAAAACCGAAGGCATCGACTTTGATGAGCGAACGCATTTTCCGCTCACGCT
CTGGGCGACACCGCATACGGCAGGCCTGACGCTGATGGCCGGTTTCAGCCGCGATATGCTCGCCTCTGAGACGGCAGCGG
CTCTGCTCGAACGACTGGGCGATATTATCGCCATCATGATCCGCTCATCCACCACAGATGTGTGCGCAATTCTGGATGAA
CTGCCTTCAGTGATGGCGGTTGCCGGTCTATCTGAGGGCGGTTCTGGGGACCGCACCCATTTGATGCCAACGAAACAGGC
ACCATCGGGCATCCCGAAATCCGCAACGCCTTCCGCGACTGAAATCACACTGGCGCAAATCTGGACGGAGGTGCTCAAAT
CTGGTCCCCTGGATGGGCAGGCCAACTTCTTTGAGCTTGGCGGCCATTCGCTCCTTGCCGTGCGTGTCGCCAGCCGACTT
CGCGGCGCGTTTTGCGTGCCTGTACCAATGCGCGTCCTTTTTGAGCACCCCGTCCTTGCCGACCTTGCGGCCTATATCGA
CAGCCTTCGCCCGCAAGTCCCCCCGGCCACAGACCATATTGAGATTGACATATGA

Upstream 100 bases:

>100_bases
GCGCCTTGAGCGCAACAACATCGCCGACATCTATCCGCTTGCCCCGATGCAGGAGGGCATCCTTTTTCATTCGGTGTCTT
CCCCCGGCGGCGGCCTCTAT

Downstream 100 bases:

>100_bases
CGATAACGCCATCACGCCCCGTGCTTCTTTGCCTGCCGCCAGCAGGCACCGGGGCCGGGCTTTTCCGCAACTGGGTACAA
ACCGCGCCGAGCACGATGAC

Product: microcystin synthetase B

Products: NA

Alternate protein names: ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent D-leucine adenylase; D-LeuA; D-Leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent glycine adenylase; GlyA; Glycine activase; Linear gramicidin--PCP reductase [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MPQTALRITGAVDAEALKAAWQGAIDRHPILRSGFFWEERDEPFQIAFRSLPVAFTTLDWTGNDPASERERLQALFSANR
AEPFDLRRPPLIRVQWISTGQDQSIMVVCYQHIILDGWSIRQLLDEVLSLYRRAAGKAEPALPPARPYGDYIGWLKKRER
AVSLRFWKDRLQGFAGTTRLLAGDATGQFERQCREVPKPLHEAVLAYGAAHGLTLNTLLQGALSLLIARQTGSRDLIFGT
TTAGRPVTLEGATTMIGLFINTLPVRVRLNEGLSVSDWLATIQKAHAETGEHDHVPLAAIQGPGVSLFDTLLVVENFPVQ
AKRADAPALLKTEGIDFDERTHFPLTLWATPHTAGLTLMAGFSRDMLASETAAALLERLGDIIAIMIRSSTTDVCAILDE
LPSVMAVAGLSEGGSGDRTHLMPTKQAPSGIPKSATPSATEITLAQIWTEVLKSGPLDGQANFFELGGHSLLAVRVASRL
RGAFCVPVPMRVLFEHPVLADLAAYIDSLRPQVPPATDHIEIDI

Sequences:

>Translated_524_residues
MPQTALRITGAVDAEALKAAWQGAIDRHPILRSGFFWEERDEPFQIAFRSLPVAFTTLDWTGNDPASERERLQALFSANR
AEPFDLRRPPLIRVQWISTGQDQSIMVVCYQHIILDGWSIRQLLDEVLSLYRRAAGKAEPALPPARPYGDYIGWLKKRER
AVSLRFWKDRLQGFAGTTRLLAGDATGQFERQCREVPKPLHEAVLAYGAAHGLTLNTLLQGALSLLIARQTGSRDLIFGT
TTAGRPVTLEGATTMIGLFINTLPVRVRLNEGLSVSDWLATIQKAHAETGEHDHVPLAAIQGPGVSLFDTLLVVENFPVQ
AKRADAPALLKTEGIDFDERTHFPLTLWATPHTAGLTLMAGFSRDMLASETAAALLERLGDIIAIMIRSSTTDVCAILDE
LPSVMAVAGLSEGGSGDRTHLMPTKQAPSGIPKSATPSATEITLAQIWTEVLKSGPLDGQANFFELGGHSLLAVRVASRL
RGAFCVPVPMRVLFEHPVLADLAAYIDSLRPQVPPATDHIEIDI
>Mature_523_residues
PQTALRITGAVDAEALKAAWQGAIDRHPILRSGFFWEERDEPFQIAFRSLPVAFTTLDWTGNDPASERERLQALFSANRA
EPFDLRRPPLIRVQWISTGQDQSIMVVCYQHIILDGWSIRQLLDEVLSLYRRAAGKAEPALPPARPYGDYIGWLKKRERA
VSLRFWKDRLQGFAGTTRLLAGDATGQFERQCREVPKPLHEAVLAYGAAHGLTLNTLLQGALSLLIARQTGSRDLIFGTT
TAGRPVTLEGATTMIGLFINTLPVRVRLNEGLSVSDWLATIQKAHAETGEHDHVPLAAIQGPGVSLFDTLLVVENFPVQA
KRADAPALLKTEGIDFDERTHFPLTLWATPHTAGLTLMAGFSRDMLASETAAALLERLGDIIAIMIRSSTTDVCAILDEL
PSVMAVAGLSEGGSGDRTHLMPTKQAPSGIPKSATPSATEITLAQIWTEVLKSGPLDGQANFFELGGHSLLAVRVASRLR
GAFCVPVPMRVLFEHPVLADLAAYIDSLRPQVPPATDHIEIDI

Specific function: Activates the 13th to the 16th (Trp, D-Leu, Trp and Gly) amino acids in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 14th (D-Leu) amino acid. It also catalyzes

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR013120
- InterPro:   IPR016040
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR010080 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF07993 NAD_binding_4; PF00550 PP-binding [H]

EC number: NA

Molecular weight: Translated: 57210; Mature: 57079

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTALRITGAVDAEALKAAWQGAIDRHPILRSGFFWEERDEPFQIAFRSLPVAFTTLDW
CCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCEEEEEEEC
TGNDPASERERLQALFSANRAEPFDLRRPPLIRVQWISTGQDQSIMVVCYQHIILDGWSI
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHH
RQLLDEVLSLYRRAAGKAEPALPPARPYGDYIGWLKKRERAVSLRFWKDRLQGFAGTTRL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LAGDATGQFERQCREVPKPLHEAVLAYGAAHGLTLNTLLQGALSLLIARQTGSRDLIFGT
EECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
TTAGRPVTLEGATTMIGLFINTLPVRVRLNEGLSVSDWLATIQKAHAETGEHDHVPLAAI
CCCCCEEEECCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
QGPGVSLFDTLLVVENFPVQAKRADAPALLKTEGIDFDERTHFPLTLWATPHTAGLTLMA
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCEEEEE
GFSRDMLASETAAALLERLGDIIAIMIRSSTTDVCAILDELPSVMAVAGLSEGGSGDRTH
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LMPTKQAPSGIPKSATPSATEITLAQIWTEVLKSGPLDGQANFFELGGHSLLAVRVASRL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHH
RGAFCVPVPMRVLFEHPVLADLAAYIDSLRPQVPPATDHIEIDI
CCCEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEC
>Mature Secondary Structure 
PQTALRITGAVDAEALKAAWQGAIDRHPILRSGFFWEERDEPFQIAFRSLPVAFTTLDW
CCCEEEEECCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCEEEEEEEC
TGNDPASERERLQALFSANRAEPFDLRRPPLIRVQWISTGQDQSIMVVCYQHIILDGWSI
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHH
RQLLDEVLSLYRRAAGKAEPALPPARPYGDYIGWLKKRERAVSLRFWKDRLQGFAGTTRL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LAGDATGQFERQCREVPKPLHEAVLAYGAAHGLTLNTLLQGALSLLIARQTGSRDLIFGT
EECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
TTAGRPVTLEGATTMIGLFINTLPVRVRLNEGLSVSDWLATIQKAHAETGEHDHVPLAAI
CCCCCEEEECCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
QGPGVSLFDTLLVVENFPVQAKRADAPALLKTEGIDFDERTHFPLTLWATPHTAGLTLMA
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCEEEEE
GFSRDMLASETAAALLERLGDIIAIMIRSSTTDVCAILDELPSVMAVAGLSEGGSGDRTH
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LMPTKQAPSGIPKSATPSATEITLAQIWTEVLKSGPLDGQANFFELGGHSLLAVRVASRL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHH
RGAFCVPVPMRVLFEHPVLADLAAYIDSLRPQVPPATDHIEIDI
CCCEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA