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The map label for this gene is hipO [H]

Identifier: 222102578

GI number: 222102578

Start: 184832

End: 186025

Strand: Reverse

Name: hipO [H]

Synonym: Avi_7215

Alternate gene names: 222102578

Gene position: 186025-184832 (Counterclockwise)

Preceding gene: 222102579

Following gene: 222102577

Centisome position: 29.44

GC content: 59.13

Gene sequence:

>1194_bases
GTGACCTCCTCTCCAGACCCACTACCGTCTCCACATGTGATTGATCGCATAGCCAGCTATACACCAGAGCTGACAGCTAT
CCGCCACGACATTCACCAGCACCCGGAAATCGGGTTCGAAGAGGTCCGAACGTCAGGCATTGTCGCCGAAAAGCTCACCT
CATGGGGCATTGAGGTGCATCGCGGCTATGGCAAAACCGGCGTGGTGGGACGCTTGCGCGGCAAACACGCTGGCAACCGC
TCCATAGGGCTGCGCGCGGATATGGATGCCCTGCCGATGCCAGAGGAAACAGGGCTGCCGTTTGCCTCCGTCTATCCCGG
CAAGTTTCACGGCTGCGGCCATGATGTGCACACCACCATCTTGCTGGGCGCGGCCCGCTATCTGGCAGAAACCCGCGATT
TTGCGGGCACGGTCACCTTCATTTTTCAGCCCGCCGAGGAAGGGCTTGGCGGCGCGAGGGCGATGATTGCTGATGGTTTG
TTCAAGGATTTTCCGGTTGATGAAATCTATGGGCTGCACAATTCATCCTATTCTCCACCCAATCACTTGAGAGTATCGCC
GGGTGCCGCTATGGCCGGGGCCGATTTCTTTGATATCACCTTGCGCGGCAAGGGTGCGCATGCTGCCCATCCCGATTTGG
GGCGTGATCCCATTCCGGCCGTTGGTGAGTTGATTCAGGCCCTACAAACCATTGTGAGCCGCAATGTTCCGCCCACCGAT
CCTGCCGTGTTGTCGATCACCCGGATTGAAGGCGGATCAGCCTATAATGTCATTCCCGAAACCGCCTCGATTGCCGGAAC
GGTACGGGCCTTTTCCGATGAGGTGCGGCAAACCATTCGCACCCGCATCACCGAAATTTCCAATCACATTGCCGCCGCTC
ACGGGCTGACAGCCACGGTGGATATTCGCGACATTTTCTCCGTGCTCACCAACAATGACACAGCAGTTGAGGCTGTGGTG
TCTGTTGCCCGCGAGGTGCTGGGGGATGCACGGGTCTCCACCCAACCCTATCGCACCATGGGCAGCGAGGATTTCGCCGA
CTTGCTCAAGCACGCCCCCGGTGCGTTTTTCACCCTCGGCCACAGCGGCACGGTGCCCGCCCACAATCCCGGCTTCATTG
TTGATGATGCCATTCTGCCCGTCGGGGCAACCCTGTTTTCCCGGCTGGTGGAAAGCAGGCTGAAGGCCGCCTGA

Upstream 100 bases:

>100_bases
GGGTTGCGGCTCTGAACGGCAATGTCATTCATCAGCCACGGGCGCAAGCGTCCTTGGCCCTCGCCCATTTTATCAAGCCC
CTTCATCCAAGGATAGATTT

Downstream 100 bases:

>100_bases
TCAAAACAAACCCTTTCAAAAACACAATAAATGAGGACACCCATGACCATCACCCTAACCCGCCGCAAGGCTTTGTTGCT
CGGTGCCGCCCTTGGTGGCT

Product: hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTSSPDPLPSPHVIDRIASYTPELTAIRHDIHQHPEIGFEEVRTSGIVAEKLTSWGIEVHRGYGKTGVVGRLRGKHAGNR
SIGLRADMDALPMPEETGLPFASVYPGKFHGCGHDVHTTILLGAARYLAETRDFAGTVTFIFQPAEEGLGGARAMIADGL
FKDFPVDEIYGLHNSSYSPPNHLRVSPGAAMAGADFFDITLRGKGAHAAHPDLGRDPIPAVGELIQALQTIVSRNVPPTD
PAVLSITRIEGGSAYNVIPETASIAGTVRAFSDEVRQTIRTRITEISNHIAAAHGLTATVDIRDIFSVLTNNDTAVEAVV
SVAREVLGDARVSTQPYRTMGSEDFADLLKHAPGAFFTLGHSGTVPAHNPGFIVDDAILPVGATLFSRLVESRLKAA

Sequences:

>Translated_397_residues
MTSSPDPLPSPHVIDRIASYTPELTAIRHDIHQHPEIGFEEVRTSGIVAEKLTSWGIEVHRGYGKTGVVGRLRGKHAGNR
SIGLRADMDALPMPEETGLPFASVYPGKFHGCGHDVHTTILLGAARYLAETRDFAGTVTFIFQPAEEGLGGARAMIADGL
FKDFPVDEIYGLHNSSYSPPNHLRVSPGAAMAGADFFDITLRGKGAHAAHPDLGRDPIPAVGELIQALQTIVSRNVPPTD
PAVLSITRIEGGSAYNVIPETASIAGTVRAFSDEVRQTIRTRITEISNHIAAAHGLTATVDIRDIFSVLTNNDTAVEAVV
SVAREVLGDARVSTQPYRTMGSEDFADLLKHAPGAFFTLGHSGTVPAHNPGFIVDDAILPVGATLFSRLVESRLKAA
>Mature_396_residues
TSSPDPLPSPHVIDRIASYTPELTAIRHDIHQHPEIGFEEVRTSGIVAEKLTSWGIEVHRGYGKTGVVGRLRGKHAGNRS
IGLRADMDALPMPEETGLPFASVYPGKFHGCGHDVHTTILLGAARYLAETRDFAGTVTFIFQPAEEGLGGARAMIADGLF
KDFPVDEIYGLHNSSYSPPNHLRVSPGAAMAGADFFDITLRGKGAHAAHPDLGRDPIPAVGELIQALQTIVSRNVPPTDP
AVLSITRIEGGSAYNVIPETASIAGTVRAFSDEVRQTIRTRITEISNHIAAAHGLTATVDIRDIFSVLTNNDTAVEAVVS
VAREVLGDARVSTQPYRTMGSEDFADLLKHAPGAFFTLGHSGTVPAHNPGFIVDDAILPVGATLFSRLVESRLKAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=426, Percent_Identity=26.5258215962441, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787598, Length=298, Percent_Identity=28.5234899328859, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42349; Mature: 42218

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSPDPLPSPHVIDRIASYTPELTAIRHDIHQHPEIGFEEVRTSGIVAEKLTSWGIEVH
CCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
RGYGKTGVVGRLRGKHAGNRSIGLRADMDALPMPEETGLPFASVYPGKFHGCGHDVHTTI
CCCCCCCCEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHH
LLGAARYLAETRDFAGTVTFIFQPAEEGLGGARAMIADGLFKDFPVDEIYGLHNSSYSPP
HHHHHHHHHHHHHCCCEEEEEEECHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCC
NHLRVSPGAAMAGADFFDITLRGKGAHAAHPDLGRDPIPAVGELIQALQTIVSRNVPPTD
CCEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
PAVLSITRIEGGSAYNVIPETASIAGTVRAFSDEVRQTIRTRITEISNHIAAAHGLTATV
CCEEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
DIRDIFSVLTNNDTAVEAVVSVAREVLGDARVSTQPYRTMGSEDFADLLKHAPGAFFTLG
EHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHCCCCEEEEC
HSGTVPAHNPGFIVDDAILPVGATLFSRLVESRLKAA
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSSPDPLPSPHVIDRIASYTPELTAIRHDIHQHPEIGFEEVRTSGIVAEKLTSWGIEVH
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
RGYGKTGVVGRLRGKHAGNRSIGLRADMDALPMPEETGLPFASVYPGKFHGCGHDVHTTI
CCCCCCCCEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHH
LLGAARYLAETRDFAGTVTFIFQPAEEGLGGARAMIADGLFKDFPVDEIYGLHNSSYSPP
HHHHHHHHHHHHHCCCEEEEEEECHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCC
NHLRVSPGAAMAGADFFDITLRGKGAHAAHPDLGRDPIPAVGELIQALQTIVSRNVPPTD
CCEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
PAVLSITRIEGGSAYNVIPETASIAGTVRAFSDEVRQTIRTRITEISNHIAAAHGLTATV
CCEEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
DIRDIFSVLTNNDTAVEAVVSVAREVLGDARVSTQPYRTMGSEDFADLLKHAPGAFFTLG
EHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHCCCCEEEEC
HSGTVPAHNPGFIVDDAILPVGATLFSRLVESRLKAA
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]