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The map label for this gene is gsiB [H]

Identifier: 222102560

GI number: 222102560

Start: 159867

End: 161453

Strand: Reverse

Name: gsiB [H]

Synonym: Avi_7194

Alternate gene names: 222102560

Gene position: 161453-159867 (Counterclockwise)

Preceding gene: 222102561

Following gene: 222102559

Centisome position: 25.56

GC content: 55.64

Gene sequence:

>1587_bases
ATGATCAAAACGCATATTGCAGGCCTCGCCGCCGCATTTCTGGGCCTATCTCTGCTGGCAACAACAGCGGATGCTGCCAC
CCTTCGCTGGGCGCGCGCATCGGACGCGCTGACACTCGACCCGAATGCCCAAAATGATGGTGTGACTGGTGCGTTGCTGG
ACCAGATCTATGACACGCTTGTCACCCGCGATGCCAAGGGCGCGTTGGAGCCAAGGCTTGCGACAGAATGGAAGGTTAAG
GATGGCGCTCCGGCAACCTGGGTGTTCACCATCCGCAAGGGCGTCAAGTTTCATGACGGCTCCGCTCTGACGGCAGAAGA
TGTGGTGTTTTCCATCAATCGCGCCCGCTCGGACAAATCCAAGCTGCGGCAATTGCATGCCGATGTTGTGGATGTAAAGG
CAGTTGGCGAGGATAAGGTTGAGGTGACGTTGCGTGCCCCCAACCTGATCTATCCCAACAACCTCACAGGCACCGATATT
CTCAGCAAGAGCTGGGCAGAAAAGAACAATGCGCTGGAGGTGCAAGATGCCGCAGCCTCCAAAGACAATTTTGCTGTGCG
CAACGCCAATGGCAGCGGACCCTATATTCTGAAATCCCGTGAAGTGGGTGTTCGCACCGTACTAGAAGCCAACCCTGCCT
ATTGGGGTGAGAAAAATCAGGGCATTACGGAAATCGTCTACACGCCGATTGCCGACAACGCCACGCGCATTGCCGCCTTG
CTCTCCGGCGAAGTGGATTTTGTGCAGGATGTGCCTGTGCAGGATATTGAGCGCCTGAAGAAAACATCCGGTGTCAAACT
GCTGACCACCCCGGAAAATCGCTCGATCTTCCTCGGCTATCGATTGGACAAGGCCCCTCTGGCATCGTCGAATGCCAAGG
ACAAGAACCCGTTGAGCGACCTGCGCGTGCGCGAAGCCTTTGATCTGGCCATTGACCGCGAAGCCCTGAAAACCGTGGTG
CTGCGTGGCAATGCCGTGCCAACCGGCATTATTGTTCCGCCGTTTGTGCATGGCTGGACACCGGCGCTGGACACCTATTC
CAAGCCAGACATTGCCAAAGCAAAGGCACTGCTGACAGAAGCTGGCTACCCGGACGGCTTCACCATCACGCTGGATACGC
CCAACAACCGCTATGTGAATGACGAGGCGATTTCTCAAGCCGTCGTCGGCTTTCTGGGTCGCATTGGCGTGAAGGTCACG
CTGGCATCACGGCCCTTCACCCAGCATTCTCCGCTGCTGGCAGATCGGAAATCTGACTTCTTCCTCTACGGCTGGGGTGT
TCCGACCTATGATAGTGCCTATAATTTCAACGACCTGATCCATTCGCGCAACGATCACTACGGCGCGTGGAATGCCACCT
ATTTCTCGGATGCCGCCATTGATGCGAAAATCGAATCTCTGGGAACAGAGGTGGATGTGGCCAAGCGCGATAAAACCATC
TCTGAGCTTTGGACCTACGTGAAGGACCAGCATCTATATCTGCCGCTGCACAATCAGATCATTGCCTGGGCTGTGCGCGA
CGGTTTTGGTCTTGAAATTCCAACCAATAACACACCTCGGTTTAACCGCGGCACAACAGGCAGTTAA

Upstream 100 bases:

>100_bases
TATAAATACATATAAACTATATATTTTATCATCAATTAACCTCTTGGCTTTCACGGGACATGGCACCCCTGCACTGTCCC
TCACCGTGATGGAGTTATTG

Downstream 100 bases:

>100_bases
CCTTTGTCACCCCGCCCAATGGGGGTGGGATAATCTTGTAAGGCAATACATTGACAGCGCCGAGCGGTTTCTCAAACGCC
CGGCGCTGGATGATTGGATA

Product: ABC transporter substrate binding protein (oligopeptide)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MIKTHIAGLAAAFLGLSLLATTADAATLRWARASDALTLDPNAQNDGVTGALLDQIYDTLVTRDAKGALEPRLATEWKVK
DGAPATWVFTIRKGVKFHDGSALTAEDVVFSINRARSDKSKLRQLHADVVDVKAVGEDKVEVTLRAPNLIYPNNLTGTDI
LSKSWAEKNNALEVQDAAASKDNFAVRNANGSGPYILKSREVGVRTVLEANPAYWGEKNQGITEIVYTPIADNATRIAAL
LSGEVDFVQDVPVQDIERLKKTSGVKLLTTPENRSIFLGYRLDKAPLASSNAKDKNPLSDLRVREAFDLAIDREALKTVV
LRGNAVPTGIIVPPFVHGWTPALDTYSKPDIAKAKALLTEAGYPDGFTITLDTPNNRYVNDEAISQAVVGFLGRIGVKVT
LASRPFTQHSPLLADRKSDFFLYGWGVPTYDSAYNFNDLIHSRNDHYGAWNATYFSDAAIDAKIESLGTEVDVAKRDKTI
SELWTYVKDQHLYLPLHNQIIAWAVRDGFGLEIPTNNTPRFNRGTTGS

Sequences:

>Translated_528_residues
MIKTHIAGLAAAFLGLSLLATTADAATLRWARASDALTLDPNAQNDGVTGALLDQIYDTLVTRDAKGALEPRLATEWKVK
DGAPATWVFTIRKGVKFHDGSALTAEDVVFSINRARSDKSKLRQLHADVVDVKAVGEDKVEVTLRAPNLIYPNNLTGTDI
LSKSWAEKNNALEVQDAAASKDNFAVRNANGSGPYILKSREVGVRTVLEANPAYWGEKNQGITEIVYTPIADNATRIAAL
LSGEVDFVQDVPVQDIERLKKTSGVKLLTTPENRSIFLGYRLDKAPLASSNAKDKNPLSDLRVREAFDLAIDREALKTVV
LRGNAVPTGIIVPPFVHGWTPALDTYSKPDIAKAKALLTEAGYPDGFTITLDTPNNRYVNDEAISQAVVGFLGRIGVKVT
LASRPFTQHSPLLADRKSDFFLYGWGVPTYDSAYNFNDLIHSRNDHYGAWNATYFSDAAIDAKIESLGTEVDVAKRDKTI
SELWTYVKDQHLYLPLHNQIIAWAVRDGFGLEIPTNNTPRFNRGTTGS
>Mature_528_residues
MIKTHIAGLAAAFLGLSLLATTADAATLRWARASDALTLDPNAQNDGVTGALLDQIYDTLVTRDAKGALEPRLATEWKVK
DGAPATWVFTIRKGVKFHDGSALTAEDVVFSINRARSDKSKLRQLHADVVDVKAVGEDKVEVTLRAPNLIYPNNLTGTDI
LSKSWAEKNNALEVQDAAASKDNFAVRNANGSGPYILKSREVGVRTVLEANPAYWGEKNQGITEIVYTPIADNATRIAAL
LSGEVDFVQDVPVQDIERLKKTSGVKLLTTPENRSIFLGYRLDKAPLASSNAKDKNPLSDLRVREAFDLAIDREALKTVV
LRGNAVPTGIIVPPFVHGWTPALDTYSKPDIAKAKALLTEAGYPDGFTITLDTPNNRYVNDEAISQAVVGFLGRIGVKVT
LASRPFTQHSPLLADRKSDFFLYGWGVPTYDSAYNFNDLIHSRNDHYGAWNATYFSDAAIDAKIESLGTEVDVAKRDKTI
SELWTYVKDQHLYLPLHNQIIAWAVRDGFGLEIPTNNTPRFNRGTTGS

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=474, Percent_Identity=28.2700421940928, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1789397, Length=523, Percent_Identity=25.8126195028681, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789966, Length=478, Percent_Identity=26.3598326359833, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081878, Length=452, Percent_Identity=25.2212389380531, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1787495, Length=502, Percent_Identity=25.0996015936255, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787762, Length=477, Percent_Identity=24.9475890985325, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789887, Length=470, Percent_Identity=24.468085106383, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787551, Length=496, Percent_Identity=23.7903225806452, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57778; Mature: 57778

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTHIAGLAAAFLGLSLLATTADAATLRWARASDALTLDPNAQNDGVTGALLDQIYDTL
CCCHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHH
VTRDAKGALEPRLATEWKVKDGAPATWVFTIRKGVKFHDGSALTAEDVVFSINRARSDKS
HCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECCCEECCCCCCCHHHHEEEHHHHCCHHH
KLRQLHADVVDVKAVGEDKVEVTLRAPNLIYPNNLTGTDILSKSWAEKNNALEVQDAAAS
HHHHHHCCCEEEEECCCCEEEEEEECCCEECCCCCCCHHHHHHHHHCCCCCEEEECCCCC
KDNFAVRNANGSGPYILKSREVGVRTVLEANPAYWGEKNQGITEIVYTPIADNATRIAAL
CCCEEEECCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
LSGEVDFVQDVPVQDIERLKKTSGVKLLTTPENRSIFLGYRLDKAPLASSNAKDKNPLSD
HCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCHHH
LRVREAFDLAIDREALKTVVLRGNAVPTGIIVPPFVHGWTPALDTYSKPDIAKAKALLTE
HHHHHHHHHHHCHHHHHEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AGYPDGFTITLDTPNNRYVNDEAISQAVVGFLGRIGVKVTLASRPFTQHSPLLADRKSDF
CCCCCCEEEEEECCCCCEECHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCE
FLYGWGVPTYDSAYNFNDLIHSRNDHYGAWNATYFSDAAIDAKIESLGTEVDVAKRDKTI
EEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHCCCCEEHHHHCCHH
SELWTYVKDQHLYLPLHNQIIAWAVRDGFGLEIPTNNTPRFNRGTTGS
HHHHHHHCCCEEEEEECCCEEEEEEECCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKTHIAGLAAAFLGLSLLATTADAATLRWARASDALTLDPNAQNDGVTGALLDQIYDTL
CCCHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHH
VTRDAKGALEPRLATEWKVKDGAPATWVFTIRKGVKFHDGSALTAEDVVFSINRARSDKS
HCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECCCEECCCCCCCHHHHEEEHHHHCCHHH
KLRQLHADVVDVKAVGEDKVEVTLRAPNLIYPNNLTGTDILSKSWAEKNNALEVQDAAAS
HHHHHHCCCEEEEECCCCEEEEEEECCCEECCCCCCCHHHHHHHHHCCCCCEEEECCCCC
KDNFAVRNANGSGPYILKSREVGVRTVLEANPAYWGEKNQGITEIVYTPIADNATRIAAL
CCCEEEECCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
LSGEVDFVQDVPVQDIERLKKTSGVKLLTTPENRSIFLGYRLDKAPLASSNAKDKNPLSD
HCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCHHH
LRVREAFDLAIDREALKTVVLRGNAVPTGIIVPPFVHGWTPALDTYSKPDIAKAKALLTE
HHHHHHHHHHHCHHHHHEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AGYPDGFTITLDTPNNRYVNDEAISQAVVGFLGRIGVKVTLASRPFTQHSPLLADRKSDF
CCCCCCEEEEEECCCCCEECHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCE
FLYGWGVPTYDSAYNFNDLIHSRNDHYGAWNATYFSDAAIDAKIESLGTEVDVAKRDKTI
EEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHCCCCEEHHHHCCHH
SELWTYVKDQHLYLPLHNQIIAWAVRDGFGLEIPTNNTPRFNRGTTGS
HHHHHHHCCCEEEEEECCCEEEEEEECCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA