BacMap

Definition	Agrobacterium vitis S4 plasmid pAtS4e, complete sequence.
Accession	NC_011981
Length	631,775

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The map label for this gene is livF [H]

Identifier: 222102533

GI number: 222102533

Start: 129417

End: 130217

Strand: Direct

Name: livF [H]

Synonym: Avi_7161

Alternate gene names: 222102533

Gene position: 129417-130217 (Clockwise)

Preceding gene: 222102532

Following gene: 222102534

Centisome position: 20.48

GC content: 56.43

Gene sequence:

>801_bases
ATGATGTCATTTGCGCTTGAGCCAACTGAGAAACAGCCGGAAGTGACTGATATTGTTGCAGATAATCTTCTGGACGTGTC
CGGCATTGAAGTTGTCTATGGCGGTGCCATTCTGGCGGTTGCAGATGTTTCGCTCACCGTTGGCGCAGGCGAGATTGTGG
CATTGCTTGGTGCCAATGGTGCCGGCAAAAGCACGGTTTTAAAGGCGATTTCCGGTCTGGTGAGCGCTGACAGGGCAACC
GTGCGTAAAGGCCACATCCGCTTTGATGACAGTGATATTCTCGGCTCTGCTGCCAATCATCTCGCAGCACGGGGCATTGT
CCATGTGCTGGAAGGCCGCCATGTGTTTTCTCATCTGACGGTTGAGGAAAATCTACAGGCAGGCGCATTCCTGCACAGGC
CTCGCCGCCGCGATCTGTTGCGGCAGATTGAGGAGGTCTACGATTGGTTTCCGAGGCTCAAGGAAAAGCGGAAGACACTG
GCTGGTCTGACATCCGGCGGTGAGCAGCAGATGCTGGCCATTGGCCGCGCTCTTCTCACCCAGCCCCGGCTGGTGTTGCT
GGACGAGCCATCCATGGGTCTGGCCCCGCGCATTGTGCAGGAAATTTTCGAGATCATCGGCAGGCTCAACCGTGAAAAAG
GGGTGGCCTTTCTGCTTGCCGAGCAAAACGCCGCCTTGTCGCTGGCCCATTCCAGCCGCGCTTACATTCTTGAAAGCGGC
AAGGTGGCTTTGGTGGGAACCTCTGCTGAGCTGACTGCGCGCGATGATCTTCACCACATCTATCTCGGAGCGTCCGCTTA
A

Upstream 100 bases:

>100_bases
GGGAAAAGTGGAACCCGGTTTTCCCGAAAAGACAAACGAAAACAAAAGAATCGAGAGTCTGTTGGGTTCAATCTGAACCA
GACAGACACGAAGGAGATGG

Downstream 100 bases:

>100_bases
ATCCGGACACCAACAACGACCAACAGGAAAAGCATCTTCATGTCACAAATTGATCTAGGCTGGGGCGAGGGCCCTTCAGA
GCGCTATGAAAGCCTTGCCG

Product: ABC transporter nucleotide binding/ATPase protein (branched chain amino acid)

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein F [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MMSFALEPTEKQPEVTDIVADNLLDVSGIEVVYGGAILAVADVSLTVGAGEIVALLGANGAGKSTVLKAISGLVSADRAT
VRKGHIRFDDSDILGSAANHLAARGIVHVLEGRHVFSHLTVEENLQAGAFLHRPRRRDLLRQIEEVYDWFPRLKEKRKTL
AGLTSGGEQQMLAIGRALLTQPRLVLLDEPSMGLAPRIVQEIFEIIGRLNREKGVAFLLAEQNAALSLAHSSRAYILESG
KVALVGTSAELTARDDLHHIYLGASA

Sequences:

>Translated_266_residues
MMSFALEPTEKQPEVTDIVADNLLDVSGIEVVYGGAILAVADVSLTVGAGEIVALLGANGAGKSTVLKAISGLVSADRAT
VRKGHIRFDDSDILGSAANHLAARGIVHVLEGRHVFSHLTVEENLQAGAFLHRPRRRDLLRQIEEVYDWFPRLKEKRKTL
AGLTSGGEQQMLAIGRALLTQPRLVLLDEPSMGLAPRIVQEIFEIIGRLNREKGVAFLLAEQNAALSLAHSSRAYILESG
KVALVGTSAELTARDDLHHIYLGASA
>Mature_266_residues
MMSFALEPTEKQPEVTDIVADNLLDVSGIEVVYGGAILAVADVSLTVGAGEIVALLGANGAGKSTVLKAISGLVSADRAT
VRKGHIRFDDSDILGSAANHLAARGIVHVLEGRHVFSHLTVEENLQAGAFLHRPRRRDLLRQIEEVYDWFPRLKEKRKTL
AGLTSGGEQQMLAIGRALLTQPRLVLLDEPSMGLAPRIVQEIFEIIGRLNREKGVAFLLAEQNAALSLAHSSRAYILESG
KVALVGTSAELTARDDLHHIYLGASA

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27477115, Length=218, Percent_Identity=28.8990825688073, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI6005701, Length=218, Percent_Identity=29.3577981651376, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27262624, Length=248, Percent_Identity=26.2096774193548, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI27262626, Length=248, Percent_Identity=26.2096774193548, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI27881501, Length=218, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI30795238, Length=218, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI105990541, Length=251, Percent_Identity=29.0836653386454, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI153792144, Length=224, Percent_Identity=27.6785714285714, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI27436953, Length=217, Percent_Identity=31.3364055299539, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45446740, Length=222, Percent_Identity=29.2792792792793, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI47078218, Length=222, Percent_Identity=29.2792792792793, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI9961250, Length=158, Percent_Identity=32.9113924050633, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4505771, Length=158, Percent_Identity=32.9113924050633, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI9961252, Length=158, Percent_Identity=32.9113924050633, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI21536376, Length=225, Percent_Identity=28, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI42741659, Length=169, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI255708477, Length=163, Percent_Identity=31.2883435582822, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI11967971, Length=238, Percent_Identity=27.3109243697479, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI116734710, Length=238, Percent_Identity=25.6302521008403, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI148612844, Length=239, Percent_Identity=28.8702928870293, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI87082268, Length=229, Percent_Identity=49.3449781659389, Blast_Score=194, Evalue=3e-51,
Organism=Escherichia coli, GI1789864, Length=259, Percent_Identity=31.2741312741313, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789593, Length=228, Percent_Identity=34.2105263157895, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787370, Length=236, Percent_Identity=30.0847457627119, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790467, Length=220, Percent_Identity=29.5454545454545, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081791, Length=234, Percent_Identity=31.1965811965812, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787089, Length=230, Percent_Identity=29.1304347826087, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790544, Length=247, Percent_Identity=27.9352226720648, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87081834, Length=204, Percent_Identity=31.3725490196078, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788225, Length=223, Percent_Identity=28.6995515695067, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787112, Length=275, Percent_Identity=32.7272727272727, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790190, Length=236, Percent_Identity=26.6949152542373, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081709, Length=217, Percent_Identity=24.8847926267281, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787712, Length=226, Percent_Identity=25.6637168141593, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1790525, Length=246, Percent_Identity=28.8617886178862, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789672, Length=240, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI48995001, Length=235, Percent_Identity=29.3617021276596, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788761, Length=225, Percent_Identity=27.1111111111111, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787029, Length=209, Percent_Identity=24.8803827751196, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787792, Length=250, Percent_Identity=26.8, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786398, Length=186, Percent_Identity=30.6451612903226, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082267, Length=234, Percent_Identity=23.9316239316239, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788897, Length=217, Percent_Identity=29.0322580645161, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788165, Length=220, Percent_Identity=26.3636363636364, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786253, Length=215, Percent_Identity=27.4418604651163, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI48994997, Length=251, Percent_Identity=29.8804780876494, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787105, Length=203, Percent_Identity=29.064039408867, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI48994943, Length=222, Percent_Identity=26.1261261261261, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788450, Length=233, Percent_Identity=27.0386266094421, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788506, Length=224, Percent_Identity=29.4642857142857, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788472, Length=204, Percent_Identity=28.921568627451, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081782, Length=240, Percent_Identity=29.5833333333333, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786872, Length=216, Percent_Identity=28.2407407407407, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1790162, Length=233, Percent_Identity=24.8927038626609, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI48994883, Length=253, Percent_Identity=24.901185770751, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786319, Length=204, Percent_Identity=30.3921568627451, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789873, Length=212, Percent_Identity=30.188679245283, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786803, Length=239, Percent_Identity=25.9414225941423, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788210, Length=249, Percent_Identity=26.9076305220884, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789586, Length=237, Percent_Identity=25.7383966244726, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789991, Length=206, Percent_Identity=30.0970873786408, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786563, Length=221, Percent_Identity=28.5067873303167, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789032, Length=229, Percent_Identity=27.0742358078603, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1787500, Length=232, Percent_Identity=26.7241379310345, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787759, Length=240, Percent_Identity=25.4166666666667, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=217, Percent_Identity=32.7188940092166, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17510237, Length=252, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25146777, Length=221, Percent_Identity=27.6018099547511, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193211017, Length=225, Percent_Identity=27.5555555555556, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71996809, Length=225, Percent_Identity=27.5555555555556, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193211015, Length=225, Percent_Identity=27.5555555555556, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558664, Length=226, Percent_Identity=28.3185840707965, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI212646699, Length=233, Percent_Identity=26.6094420600858, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17565938, Length=215, Percent_Identity=26.5116279069767, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17569143, Length=249, Percent_Identity=24.4979919678715, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71983516, Length=219, Percent_Identity=25.5707762557078, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71983510, Length=219, Percent_Identity=25.5707762557078, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=219, Percent_Identity=25.5707762557078, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17569145, Length=255, Percent_Identity=26.2745098039216, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=172, Percent_Identity=30.2325581395349, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71984940, Length=216, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71992285, Length=244, Percent_Identity=24.5901639344262, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324498, Length=224, Percent_Identity=29.0178571428571, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24666092, Length=248, Percent_Identity=24.5967741935484, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28574150, Length=220, Percent_Identity=28.6363636363636, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI116007328, Length=220, Percent_Identity=28.6363636363636, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28571209, Length=251, Percent_Identity=26.6932270916335, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574744, Length=215, Percent_Identity=26.046511627907, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24643674, Length=221, Percent_Identity=24.4343891402715, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24581191, Length=205, Percent_Identity=23.9024390243902, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581189, Length=205, Percent_Identity=23.9024390243902, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581193, Length=205, Percent_Identity=23.9024390243902, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581195, Length=205, Percent_Identity=23.9024390243902, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI85724864, Length=209, Percent_Identity=23.9234449760766, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24580555, Length=209, Percent_Identity=23.9234449760766, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI85816269, Length=209, Percent_Identity=23.9234449760766, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24580553, Length=209, Percent_Identity=23.9234449760766, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357907, Length=241, Percent_Identity=26.9709543568465, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 28592; Mature: 28592

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSFALEPTEKQPEVTDIVADNLLDVSGIEVVYGGAILAVADVSLTVGAGEIVALLGANG
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEECCEEEEEEEEEEEECCCCEEEEECCCC
AGKSTVLKAISGLVSADRATVRKGHIRFDDSDILGSAANHLAARGIVHVLEGRHVFSHLT
CCHHHHHHHHHHHHHCCHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
VEENLQAGAFLHRPRRRDLLRQIEEVYDWFPRLKEKRKTLAGLTSGGEQQMLAIGRALLT
HHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
QPRLVLLDEPSMGLAPRIVQEIFEIIGRLNREKGVAFLLAEQNAALSLAHSSRAYILESG
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCEEEEECC
KVALVGTSAELTARDDLHHIYLGASA
CEEEEECCCCEEECCCCEEEEEECCC
>Mature Secondary Structure
MMSFALEPTEKQPEVTDIVADNLLDVSGIEVVYGGAILAVADVSLTVGAGEIVALLGANG
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEECCEEEEEEEEEEEECCCCEEEEECCCC
AGKSTVLKAISGLVSADRATVRKGHIRFDDSDILGSAANHLAARGIVHVLEGRHVFSHLT
CCHHHHHHHHHHHHHCCHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
VEENLQAGAFLHRPRRRDLLRQIEEVYDWFPRLKEKRKTLAGLTSGGEQQMLAIGRALLT
HHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
QPRLVLLDEPSMGLAPRIVQEIFEIIGRLNREKGVAFLLAEQNAALSLAHSSRAYILESG
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCEEEEECC
KVALVGTSAELTARDDLHHIYLGASA
CEEEEECCCCEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]