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The map label for this gene is glcD [H]

Identifier: 222096971

GI number: 222096971

Start: 3253684

End: 3255075

Strand: Reverse

Name: glcD [H]

Synonym: BCQ_3311

Alternate gene names: 222096971

Gene position: 3255075-3253684 (Counterclockwise)

Preceding gene: 222096972

Following gene: 222096970

Centisome position: 62.43

GC content: 38.72

Gene sequence:

>1392_bases
ATGAAAATAACAGTAGAGCGTTTAGTAAATGAATTAAAGGGTGTACTTCCCGGCGATCGAGTAGTTGTGAATACGACAGT
AAGAGAGTTACATAGTAAAGATGAATCTTATCATGCTAGTAGTTTACCTGATGTAGTAGTTTTTCCGAAAACGACCGAAG
AGGTTAGCACGATAATGAAAATAGCGAGTGAGCACGGGACACCCGTCGTTCCGTTTGGGGTAGGTTCTAGTTTAGAAGGA
CATGTAATTCCTTATGAAAAAGGAATTACGGTTGATTTTTCATTAATGAACAAAATTCTTGAAATAAGAGAGAAAGATTT
TCTGGTGAAAGTACAGCCAGGAGTGACTCGTTCTCAGCTCAATAAAGAATTGAAGAAATACGGATTGTTTTTTAGTGTGG
ATCCAGGAGCAGATGCAACGTTAGGGGGAATGGCTGCTACAAATGCAAGCGGAACGACAGCGGTTAAGTATGGAGTAATG
CGTGATCAAGTTCGTGATTTAGAAGTCGTTCTTGCTGATGGGGAAGTAATACATACTGGGAATTTAGCTGCGAAGTCGTC
ATCAGGTTACCATTTAAATGGTGTTTTCGTAGGATCAGAAGGTACGCTTGGATGCTTTACAGAGTTAACATTAAAAGTAT
ACGGCATACCAGAGCATGTTATGGCTGCAAGAGCATCGTTTCCAACTATAAATGATGCAGTAGAAGCTGTTATTAACATA
TTGCAAGCGGGTATTCCGATTGCGAGAATTGAACTCGTTGATGAACTATCTATGAAACAAGTCAATCATTATAGTGAAAC
AAGTTACAGAGAAGAACCTACATTGTTTTTAGAGTTTCACGGTAATGAAGCAGGGTTAAAGCAAGATGTTGAATTTACGA
AGGAAATTGTTTTTGATCATAAATGTAAAGAAGTTGCTTTTGAAACGGAAACTGCAGCGAGAAATAAATTATGGGATGCA
CGGCATAATCTAGCATATGCTTACGTTCATAGCTATCCTGGTAAAAAGCTTATGAGTACAGACGTATGTGTACCAATTTC
AGAATTGGCTGGGGCAATTCAACATGCGAAAGATACATTAGATAAGGTTGGGCTTGTTGGTGGTATTCTTGGTCATGTAG
GAGACGGGAATTTCCACGTGCTCTTAATGGTTGATCCAAACGATAAAGAAGAAGTGGAAAAGGCAGACGAAATAAATGAA
AATATCGTTTTATATGCTCTTAAACGAGGAGGAACATGTACAGGGGAACATGGCGTTGGTATTGGAAAACGGAAGTATCA
GGAAGAAGAACATGGGGCGGCATTACTCGTAATGGAAAAAATAAAGAAAGCTCTTGATCCGCAAAATATTTTGAATCCGA
ATAAAGTTTTTCAGTTAAAGGATAGGAGCTGA

Upstream 100 bases:

>100_bases
ATATGTATAAAACTTGAATTATTTAATTTTTCGGATTATTATGTATAAAATAGTTAACATATTTTGTAATTCAAATTTAT
AGAGAATAGGAGAGTGTACG

Downstream 100 bases:

>100_bases
TCGGGTGAAAGTACTAGAAGCGATAAGTAATATAGCTGGAAAGTATTTTGCGTTTTGGGTTATTTGTATTGCAACTATTG
CGTATTTTATTCCCGCTCCA

Product: glycolate oxidase, glcd subunit

Products: pyruvate; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKITVERLVNELKGVLPGDRVVVNTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMKIASEHGTPVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQHAKDTLDKVGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINE
NIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALLVMEKIKKALDPQNILNPNKVFQLKDRS

Sequences:

>Translated_463_residues
MKITVERLVNELKGVLPGDRVVVNTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMKIASEHGTPVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQHAKDTLDKVGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINE
NIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALLVMEKIKKALDPQNILNPNKVFQLKDRS
>Mature_463_residues
MKITVERLVNELKGVLPGDRVVVNTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMKIASEHGTPVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQHAKDTLDKVGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINE
NIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALLVMEKIKKALDPQNILNPNKVFQLKDRS

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=454, Percent_Identity=47.5770925110132, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI37595754, Length=477, Percent_Identity=45.0733752620545, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI119964728, Length=433, Percent_Identity=26.7898383371824, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4501993, Length=219, Percent_Identity=30.1369863013699, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1789351, Length=465, Percent_Identity=30.1075268817204, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787977, Length=494, Percent_Identity=27.1255060728745, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI48994907, Length=492, Percent_Identity=25, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71992373, Length=462, Percent_Identity=43.5064935064935, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71992381, Length=462, Percent_Identity=42.6406926406926, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71992392, Length=361, Percent_Identity=47.6454293628809, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17534361, Length=453, Percent_Identity=26.9315673289183, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17556096, Length=452, Percent_Identity=26.1061946902655, Blast_Score=118, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320027, Length=474, Percent_Identity=38.1856540084388, Blast_Score=313, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6320764, Length=468, Percent_Identity=24.5726495726496, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6320023, Length=480, Percent_Identity=25.4166666666667, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18921117, Length=431, Percent_Identity=25.9860788863109, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24639277, Length=431, Percent_Identity=25.9860788863109, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24639275, Length=431, Percent_Identity=25.9860788863109, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24653753, Length=219, Percent_Identity=31.0502283105023, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.2.4

Molecular weight: Translated: 50709; Mature: 50709

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVERLVNELKGVLPGDRVVVNTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMK
CEEEHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHH
IASEHGTPVVPFGVGSSLEGHVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQL
HHHHCCCEEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCHHHH
NKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVMRDQVRDLEVVLADGEVIHTG
HHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHEEHHHHHHHHCCEEEEEECCCEEECC
NLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
CCEECCCCCEEEEEEEECCCCCEEEEEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHH
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDH
HHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KCKEVAFETETAARNKLWDARHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQHAKDTL
HHHHHHHCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
DKVGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINENIVLYALKRGGTCTGEHGVG
HHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCEECCCCCCC
IGKRKYQEEEHGAALLVMEKIKKALDPQNILNPNKVFQLKDRS
CCHHHCCHHHCCCCHHHHHHHHHHCCHHHCCCCCCEEEECCCC
>Mature Secondary Structure
MKITVERLVNELKGVLPGDRVVVNTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMK
CEEEHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHH
IASEHGTPVVPFGVGSSLEGHVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQL
HHHHCCCEEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCHHHH
NKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVMRDQVRDLEVVLADGEVIHTG
HHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHEEHHHHHHHHCCEEEEEECCCEEECC
NLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPTINDAVEAVINI
CCEECCCCCEEEEEEEECCCCCEEEEEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHH
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDH
HHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KCKEVAFETETAARNKLWDARHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQHAKDTL
HHHHHHHCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
DKVGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINENIVLYALKRGGTCTGEHGVG
HHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCEECCCCCCC
IGKRKYQEEEHGAALLVMEKIKKALDPQNILNPNKVFQLKDRS
CCHHHCCHHHCCCCHHHHHHHHHHCCHHHCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; ferricytochrome c

Specific reaction: (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]