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The map label for this gene is cobB [H]

Identifier: 222096560

GI number: 222096560

Start: 2835545

End: 2836273

Strand: Reverse

Name: cobB [H]

Synonym: BCQ_2900

Alternate gene names: 222096560

Gene position: 2836273-2835545 (Counterclockwise)

Preceding gene: 222096561

Following gene: 222096558

Centisome position: 54.4

GC content: 35.12

Gene sequence:

>729_bases
GTGCAACAATTTGAAGAAGTACGTTCAATTTTAGAAAAAGCGAAGAAAATGACAGTATTAACAGGTGCAGGTGCAAGTAC
GGAAAGTGGTATCCCAGATTTTCGTTCAGCAAATGGATTGTATGCTGATGCGAATGTGGAAATGTACTTATCAAGAGGGT
ACTATAACCGAAGTCCGAAAGAATTTTGGAAGCATTATAAAGAAATATTTCAAATCAATACGTTTCATCAATATAAGCCA
AATCGTGGACATCGCTTTTTAGCTGAATTAGAAGAAAAAGGAAAAGATATTACGATTTTAACGCAAAATATTGACGGTTT
ACATCAAGTAGGTGGTAGTAAACATGTTATTGATTTACATGGCACACTGCAAACAGCACATTGTCCGAAGTGTAAAACGG
GATATGATTTGCAGTATATGATTGATCATGAGGTGCCGCGCTGTCAGAAGTGTAATTTCATTTTAAATCCAGACGTTGTT
TTATACGGAGATACATTGCCGCAATATCAAAATGCGATAAAACGTTTATATGAAACAGATGTGCTTATCGTTATGGGAAC
GTCATTAAAAGTACAACCCGTTGCTTCATTCCCAGAAATCGCAAAGAGAGAAGTAGGAGCAACAACCATTTTAGTAAATG
AAGAGTTAACAGGACAAGAATATAACTTTGATTATGTTTTTCAAAATAAGATTGGTGAATTCGTTGAAGGATTGTCTTCA
AGGAAATAA

Upstream 100 bases:

>100_bases
GAGGTTGGTGGCACGACGCATTAAGTAGAATGAATTGTACGACACCTCCTGTAAACAACTGGTATAATATAGTATAGGAA
AATACATAGAGGTGATTGTT

Downstream 100 bases:

>100_bases
GTTATCGAAAAAACTGAGGTATCCGCCTCAGTTTTTTATTTGAGTAATCGTTATACTAAGCAGCGTGAAAACATGTCGAT
AATGTAGTAACAACATCTCT

Product: nad-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MQQFEEVRSILEKAKKMTVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPKEFWKHYKEIFQINTFHQYKP
NRGHRFLAELEEKGKDITILTQNIDGLHQVGGSKHVIDLHGTLQTAHCPKCKTGYDLQYMIDHEVPRCQKCNFILNPDVV
LYGDTLPQYQNAIKRLYETDVLIVMGTSLKVQPVASFPEIAKREVGATTILVNEELTGQEYNFDYVFQNKIGEFVEGLSS
RK

Sequences:

>Translated_242_residues
MQQFEEVRSILEKAKKMTVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPKEFWKHYKEIFQINTFHQYKP
NRGHRFLAELEEKGKDITILTQNIDGLHQVGGSKHVIDLHGTLQTAHCPKCKTGYDLQYMIDHEVPRCQKCNFILNPDVV
LYGDTLPQYQNAIKRLYETDVLIVMGTSLKVQPVASFPEIAKREVGATTILVNEELTGQEYNFDYVFQNKIGEFVEGLSS
RK
>Mature_242_residues
MQQFEEVRSILEKAKKMTVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPKEFWKHYKEIFQINTFHQYKP
NRGHRFLAELEEKGKDITILTQNIDGLHQVGGSKHVIDLHGTLQTAHCPKCKTGYDLQYMIDHEVPRCQKCNFILNPDVV
LYGDTLPQYQNAIKRLYETDVLIVMGTSLKVQPVASFPEIAKREVGATTILVNEELTGQEYNFDYVFQNKIGEFVEGLSS
RK

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI7657575, Length=234, Percent_Identity=37.6068376068376, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI13775602, Length=231, Percent_Identity=35.9307359307359, Blast_Score=135, Evalue=2e-32,
Organism=Homo sapiens, GI13775600, Length=231, Percent_Identity=35.9307359307359, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI6912660, Length=218, Percent_Identity=36.2385321100917, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI63054862, Length=213, Percent_Identity=36.6197183098592, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI6912664, Length=257, Percent_Identity=29.5719844357977, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI300795542, Length=244, Percent_Identity=30.327868852459, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI300797705, Length=203, Percent_Identity=34.9753694581281, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI13787215, Length=254, Percent_Identity=28.740157480315, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI215982798, Length=173, Percent_Identity=38.150289017341, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI6912662, Length=226, Percent_Identity=30.5309734513274, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI300797577, Length=254, Percent_Identity=26.3779527559055, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI308199517, Length=230, Percent_Identity=33.4782608695652, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17541892, Length=230, Percent_Identity=33.9130434782609, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17567771, Length=227, Percent_Identity=27.3127753303965, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71990482, Length=222, Percent_Identity=27.9279279279279, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71990487, Length=224, Percent_Identity=27.6785714285714, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324599, Length=304, Percent_Identity=28.2894736842105, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324504, Length=284, Percent_Identity=28.169014084507, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6325242, Length=234, Percent_Identity=30.3418803418803, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320163, Length=254, Percent_Identity=27.9527559055118, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320397, Length=231, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17137536, Length=250, Percent_Identity=34.4, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24648389, Length=222, Percent_Identity=34.2342342342342, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI28571445, Length=186, Percent_Identity=30.1075268817204, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24645650, Length=250, Percent_Identity=22.8, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27637; Mature: 27637

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQFEEVRSILEKAKKMTVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPK
CCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCHH
EFWKHYKEIFQINTFHQYKPNRGHRFLAELEEKGKDITILTQNIDGLHQVGGSKHVIDLH
HHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCEEEEEC
GTLQTAHCPKCKTGYDLQYMIDHEVPRCQKCNFILNPDVVLYGDTLPQYQNAIKRLYETD
CCEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHHHCC
VLIVMGTSLKVQPVASFPEIAKREVGATTILVNEELTGQEYNFDYVFQNKIGEFVEGLSS
EEEEECCCEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHC
RK
CC
>Mature Secondary Structure
MQQFEEVRSILEKAKKMTVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPK
CCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCHH
EFWKHYKEIFQINTFHQYKPNRGHRFLAELEEKGKDITILTQNIDGLHQVGGSKHVIDLH
HHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCEEEEEC
GTLQTAHCPKCKTGYDLQYMIDHEVPRCQKCNFILNPDVVLYGDTLPQYQNAIKRLYETD
CCEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHHHCC
VLIVMGTSLKVQPVASFPEIAKREVGATTILVNEELTGQEYNFDYVFQNKIGEFVEGLSS
EEEEECCCEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHC
RK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]