Click here to switch to the map view.

The map label for this gene is acoC [H]

Identifier: 222096275

GI number: 222096275

Start: 2566870

End: 2568069

Strand: Reverse

Name: acoC [H]

Synonym: BCQ_2615

Alternate gene names: 222096275

Gene position: 2568069-2566870 (Counterclockwise)

Preceding gene: 222096276

Following gene: 222096274

Centisome position: 49.25

GC content: 35.75

Gene sequence:

>1200_bases
ATGGCTGTAGAAGTTGTAATGCCGAAGTTAGGTATGGCGATGAAAGAAGGTATTATTACGAGCTGGAATATTAAAGCAGG
CGATCATGTCTCAAAAGGTGAACTAATTGCTAGTATTAATTCGGAAAAAATCGAAACGGAAATAGAAGCACCAGCTGATG
GAACTGTTCTTGATATAGCTGTTAGTGAAGATGAGGGAGTTCCACCTGGCACTGTCATTTGCTATATCGGTAAGCTGAAC
GAAAAAGTAGAAATCAATGAAAGTATAAATGTTGTAGAAGAAAAAGCACCTAATCTAGAGCCTAAAAAGGTCCAACATCC
AGAACCATATGCGAAAGAAGTGGCGAAGCAAAGAATCAAAATTTCACCTGTAGCGAAGAAAATTGCAAAGTCTGAAAATC
TAGATATCAAAGCATTAGTTGGTACAGGCCCTGGCGGAAGGATTACAAAAGTAGACGTATTAAAAGCGATTGAAGAAAGA
GTAACAATTCCAGAAGTACTTGAACAAGAAGAAAGTAAAGTAATTCCTGTTACTGGTATGCGGAAAGCAATCGCAAACCG
TATGCATGCAAGTTTACAAAATAGTGCGCAATTAACATTAACGATGAAAGTAGATGTGACAGATTTAGTTGCTTTACATA
AAGAAATAGCGGCAGTTGTACAAAAACGATACGATAACAAACTAACTATTACGGATTTCGTTTCTCGTGCTGTTGTATTA
GCACTTGGAGAGCATAAAGAAATGAACAGCGCTTATATGGATGATACAATTCATCAATTTGAACATGTTCATTTAGGCAT
GGCAGTCGCATTAGAAAAAGGATTAGTCGTTCCAGCTATTCGCTTTGCTAATAATCTATCTTTAGTAGAGTTATCTAAAC
AAATTAAAAACGTTGCACAAAAAGCAAGAGAAGGTAGTTTAAGTAGTGATGATATGCAAGGGACAACCTTTACAATTAGT
AATTTAGGTAGCTTCGGTATTGAATATTTTACGCCAGTATTAAATACACCTGAAACTGGTATTTTAGGTGTAGGTGCAAT
TGAACATGTTCCAGTATATAAAGGGAAGAAAATAAAAAAAGGCAGTATGTTACCTTTAAGTTTAACATTCGATCATCGTG
TATTAGATGGTGCACCAGCAGCTGCATTTTTACGCACAATTAAACGTTATTTAGAAGAACCTGTAACAATTCTTTTATAA

Upstream 100 bases:

>100_bases
TTTACCAACGCCGGAAAAAGTAATTGAAACTGTATCAGAAATGATCGGAGACCAAACTTTATTACATGTGTAATAAAACG
ATGAGAAGGGAGAGAGAAGC

Downstream 100 bases:

>100_bases
AGAGAAGGTGAGAGAATGAGTAAACTAGTCGTTATTGGTGGCGGACCTGCTGGCTATGTAGCGGCAATTACCGCAGCTCA
AAATGGAAAAGATGTCACTC

Product: branched-chain alpha-keto acid dehydrogenase subunit e2

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAVEVVMPKLGMAMKEGIITSWNIKAGDHVSKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKLN
EKVEINESINVVEEKAPNLEPKKVQHPEPYAKEVAKQRIKISPVAKKIAKSENLDIKALVGTGPGGRITKVDVLKAIEER
VTIPEVLEQEESKVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAAVVQKRYDNKLTITDFVSRAVVL
ALGEHKEMNSAYMDDTIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKQIKNVAQKAREGSLSSDDMQGTTFTIS
NLGSFGIEYFTPVLNTPETGILGVGAIEHVPVYKGKKIKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL

Sequences:

>Translated_399_residues
MAVEVVMPKLGMAMKEGIITSWNIKAGDHVSKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKLN
EKVEINESINVVEEKAPNLEPKKVQHPEPYAKEVAKQRIKISPVAKKIAKSENLDIKALVGTGPGGRITKVDVLKAIEER
VTIPEVLEQEESKVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAAVVQKRYDNKLTITDFVSRAVVL
ALGEHKEMNSAYMDDTIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKQIKNVAQKAREGSLSSDDMQGTTFTIS
NLGSFGIEYFTPVLNTPETGILGVGAIEHVPVYKGKKIKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
>Mature_398_residues
AVEVVMPKLGMAMKEGIITSWNIKAGDHVSKGELIASINSEKIETEIEAPADGTVLDIAVSEDEGVPPGTVICYIGKLNE
KVEINESINVVEEKAPNLEPKKVQHPEPYAKEVAKQRIKISPVAKKIAKSENLDIKALVGTGPGGRITKVDVLKAIEERV
TIPEVLEQEESKVIPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAAVVQKRYDNKLTITDFVSRAVVLA
LGEHKEMNSAYMDDTIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKQIKNVAQKAREGSLSSDDMQGTTFTISN
LGSFGIEYFTPVLNTPETGILGVGAIEHVPVYKGKKIKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=30.715935334873, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=26.4018691588785, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=26.9978401727862, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=26.9978401727862, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI19923748, Length=223, Percent_Identity=34.9775784753363, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI260898739, Length=187, Percent_Identity=29.9465240641711, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1786946, Length=410, Percent_Identity=31.7073170731707, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1786305, Length=422, Percent_Identity=28.436018957346, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=429, Percent_Identity=31.4685314685315, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=406, Percent_Identity=30.0492610837438, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=25.9433962264151, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=221, Percent_Identity=28.9592760180996, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=408, Percent_Identity=29.656862745098, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=27.5784753363229, Blast_Score=166, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24582497, Length=294, Percent_Identity=31.9727891156463, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI20129315, Length=294, Percent_Identity=31.9727891156463, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI18859875, Length=424, Percent_Identity=25.4716981132075, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24645909, Length=234, Percent_Identity=31.6239316239316, Blast_Score=128, Evalue=9e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43475; Mature: 43344

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEVVMPKLGMAMKEGIITSWNIKAGDHVSKGELIASINSEKIETEIEAPADGTVLDIA
CCEEEECHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCHHHEEEECCCCCCCEEEEE
VSEDEGVPPGTVICYIGKLNEKVEINESINVVEEKAPNLEPKKVQHPEPYAKEVAKQRIK
ECCCCCCCCCEEEEEEECCCCEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCC
ISPVAKKIAKSENLDIKALVGTGPGGRITKVDVLKAIEERVTIPEVLEQEESKVIPVTGM
CCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCEECCCHH
RKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAAVVQKRYDNKLTITDFVSRAVVL
HHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
ALGEHKEMNSAYMDDTIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKQIKNVAQ
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCHHHHHHHHHHHHHH
KAREGSLSSDDMQGTTFTISNLGSFGIEYFTPVLNTPETGILGVGAIEHVPVYKGKKIKK
HHHCCCCCCCCCCCCEEEECCCCHHCHHHHHHCCCCCCCCEEECCCHHCCCCCCCCEECC
GSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
AVEVVMPKLGMAMKEGIITSWNIKAGDHVSKGELIASINSEKIETEIEAPADGTVLDIA
CEEEECHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCHHHEEEECCCCCCCEEEEE
VSEDEGVPPGTVICYIGKLNEKVEINESINVVEEKAPNLEPKKVQHPEPYAKEVAKQRIK
ECCCCCCCCCEEEEEEECCCCEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCC
ISPVAKKIAKSENLDIKALVGTGPGGRITKVDVLKAIEERVTIPEVLEQEESKVIPVTGM
CCHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCEECCCHH
RKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAAVVQKRYDNKLTITDFVSRAVVL
HHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
ALGEHKEMNSAYMDDTIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKQIKNVAQ
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCHHHHHHHHHHHHHH
KAREGSLSSDDMQGTTFTISNLGSFGIEYFTPVLNTPETGILGVGAIEHVPVYKGKKIKK
HHHCCCCCCCCCCCCEEEECCCCHHCHHHHHHCCCCCCCCEEECCCHHCCCCCCCCEECC
GSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]