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The map label for this gene is acoL [H]

Identifier: 222096274

GI number: 222096274

Start: 2565475

End: 2566854

Strand: Reverse

Name: acoL [H]

Synonym: BCQ_2614

Alternate gene names: 222096274

Gene position: 2566854-2565475 (Counterclockwise)

Preceding gene: 222096275

Following gene: 222096271

Centisome position: 49.23

GC content: 37.75

Gene sequence:

>1380_bases
ATGAGTAAACTAGTCGTTATTGGTGGCGGACCTGCTGGCTATGTAGCGGCAATTACCGCAGCTCAAAATGGAAAAGATGT
CACTCTTATTGATGAAGCTGATCTTGGCGGGACTTGTTTAAATGTTGGGTGCATGCCTACAAAATCATTGTTAGAAAGTG
CAGAAGTGCATGACATTGTGAGAAAAGCGAATCATTATGGAGTTATACTCAATAATGGAAGTATATCAATTGATTGGAAG
CAAATGCAGGCGAGGAAATCACAAATTGTAACGCAGCTCGTTCAAGGAATTCAATATTTAATGAAGAAAAATAAAATAAA
AGTTATACAAGGTAAAGCGAACTTTGAAACGGATCATCGTGTGCGAGTTATATACGGTGATAAAGAAGATGTAGTAGATG
GAGAACAGTTCATTATAGCGGCAGGTTCAGAACCGACTGCATTACCCTTTGCTCCATTTGACGGAAAGTGGATTTTAAAT
AGTAGCCATGCAATGTCCATAGATTATATACCAAAATCATTATTAATCGTTGGCGGTGGTGTAATAGGATGCGAGTTTGC
AAGTATTTATAGTCGTCTTGGAACAAAAGTTACGATTGTTGAAATGGCACCGCAATTATTACCAGGTGAAGATGAAGATA
TCGCACACATTTTAAGAGGGAAACTAGAAAATGATGGTGTAGAAATATTTACAGGAGCAGCTTTAAAAGGATTAAATAAT
TATAAGAAGCAGGCTTCATTTGAATATGAAGGAAGTATCGAAGAAGTTAATCCAGAATTTGTTCTCGTTTCTGTCGGCCG
AAAACCACGTGTACAACAATTAGATTTAGAAAAAGCAGGCATTCAATATTCAAATAAAGGAATTTCTGTGAATGAACATA
TGCAAACCAATGTATCACATATTTATGCAGCAGGAGATGTGATTGGTGGAATCCAGCTTGCTCACGTTGCATTCCATGAA
GGAACGACGGCAGCATTACACGCGAGTGGAGAAGATGTAAAAGTAAACTACCATGCTGTACCTCGCTGTATTTATACCGC
TCCAGAAATTGCTAGTGTCGGTTTAACAGAAAAAGATGCAAGAGAGAAGTATGGTGACATACAAATTGGAGAATTTCCTT
TTACAGCAAACGGAAAAGCGCTTATTATTGGCGAACAAACAGGTAAAGTAAAAGTTATTGTGGAACCAAAATATCAAGAA
ATTGTTGGAATTTCTATTATTGGTCCTCGTGCAACAGAACTAATTGGTCAAGGAACTGTAATGATTCATACGGAAGTTAC
TGCCGATATTATGAGAGATTATATTGCAGCGCATCCAACTTTATCTGAAGCGATTCATGAAGCTTTATTGCAAGCGGTAG
GACATGCTGTACACGCTTAA

Upstream 100 bases:

>100_bases
TCGTGTATTAGATGGTGCACCAGCAGCTGCATTTTTACGCACAATTAAACGTTATTTAGAAGAACCTGTAACAATTCTTT
TATAAAGAGAAGGTGAGAGA

Downstream 100 bases:

>100_bases
ATGGAATTATAATAAACCACTATTCTCTAAATGAATAAGAGAATAGTGGTTTGAATTTTTACGGGAGAAATTGAATATCC
TATTATTTTTTTACGTCAAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of acetoin cleaving system [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSKLVVIGGGPAGYVAAITAAQNGKDVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIVRKANHYGVILNNGSISIDWK
QMQARKSQIVTQLVQGIQYLMKKNKIKVIQGKANFETDHRVRVIYGDKEDVVDGEQFIIAAGSEPTALPFAPFDGKWILN
SSHAMSIDYIPKSLLIVGGGVIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRGKLENDGVEIFTGAALKGLNN
YKKQASFEYEGSIEEVNPEFVLVSVGRKPRVQQLDLEKAGIQYSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFHE
GTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDAREKYGDIQIGEFPFTANGKALIIGEQTGKVKVIVEPKYQE
IVGISIIGPRATELIGQGTVMIHTEVTADIMRDYIAAHPTLSEAIHEALLQAVGHAVHA

Sequences:

>Translated_459_residues
MSKLVVIGGGPAGYVAAITAAQNGKDVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIVRKANHYGVILNNGSISIDWK
QMQARKSQIVTQLVQGIQYLMKKNKIKVIQGKANFETDHRVRVIYGDKEDVVDGEQFIIAAGSEPTALPFAPFDGKWILN
SSHAMSIDYIPKSLLIVGGGVIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRGKLENDGVEIFTGAALKGLNN
YKKQASFEYEGSIEEVNPEFVLVSVGRKPRVQQLDLEKAGIQYSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFHE
GTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDAREKYGDIQIGEFPFTANGKALIIGEQTGKVKVIVEPKYQE
IVGISIIGPRATELIGQGTVMIHTEVTADIMRDYIAAHPTLSEAIHEALLQAVGHAVHA
>Mature_458_residues
SKLVVIGGGPAGYVAAITAAQNGKDVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIVRKANHYGVILNNGSISIDWKQ
MQARKSQIVTQLVQGIQYLMKKNKIKVIQGKANFETDHRVRVIYGDKEDVVDGEQFIIAAGSEPTALPFAPFDGKWILNS
SHAMSIDYIPKSLLIVGGGVIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRGKLENDGVEIFTGAALKGLNNY
KKQASFEYEGSIEEVNPEFVLVSVGRKPRVQQLDLEKAGIQYSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFHEG
TTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDAREKYGDIQIGEFPFTANGKALIIGEQTGKVKVIVEPKYQEI
VGISIIGPRATELIGQGTVMIHTEVTADIMRDYIAAHPTLSEAIHEALLQAVGHAVHA

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=34.1304347826087, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI50301238, Length=444, Percent_Identity=28.3783783783784, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI33519430, Length=454, Percent_Identity=27.0925110132159, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI33519428, Length=454, Percent_Identity=27.0925110132159, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI33519426, Length=454, Percent_Identity=27.0925110132159, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI148277065, Length=462, Percent_Identity=26.8398268398268, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI148277071, Length=462, Percent_Identity=26.8398268398268, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=27.7292576419214, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI291045266, Length=461, Percent_Identity=26.2472885032538, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI291045268, Length=452, Percent_Identity=24.3362831858407, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786307, Length=449, Percent_Identity=33.184855233853, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=30.820399113082, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87082354, Length=456, Percent_Identity=27.6315789473684, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=28.2407407407407, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=36.480686695279, Blast_Score=267, Evalue=9e-72,
Organism=Caenorhabditis elegans, GI17557007, Length=470, Percent_Identity=26.5957446808511, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=444, Percent_Identity=26.1261261261261, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=444, Percent_Identity=26.1261261261261, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=473, Percent_Identity=25.369978858351, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=37.9237288135593, Blast_Score=272, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6325240, Length=481, Percent_Identity=27.4428274428274, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6325166, Length=449, Percent_Identity=27.1714922048998, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=37.037037037037, Blast_Score=268, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=27.5268817204301, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24640553, Length=465, Percent_Identity=27.5268817204301, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24640551, Length=465, Percent_Identity=27.5268817204301, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17737741, Length=466, Percent_Identity=26.8240343347639, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49455; Mature: 49324

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVVIGGGPAGYVAAITAAQNGKDVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIV
CCEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHH
RKANHYGVILNNGSISIDWKQMQARKSQIVTQLVQGIQYLMKKNKIKVIQGKANFETDHR
HCCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCE
VRVIYGDKEDVVDGEQFIIAAGSEPTALPFAPFDGKWILNSSHAMSIDYIPKSLLIVGGG
EEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEECCC
VIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRGKLENDGVEIFTGAALKGLNN
EEEHHHHHHHHHHCCEEEEEECCHHHCCCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHH
YKKQASFEYEGSIEEVNPEFVLVSVGRKPRVQQLDLEKAGIQYSNKGISVNEHMQTNVSH
HHHHCCCEECCCHHHCCCCEEEEECCCCCCCCEECHHHHCCEECCCCCCCCHHHHCCCCE
IYAAGDVIGGIQLAHVAFHEGTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDA
EEECCCHHCCEEEEEEEEECCCEEEEEECCCEEEEEEEECCEEEECCCCHHHCCCCCHHH
REKYGDIQIGEFPFTANGKALIIGEQTGKVKVIVEPKYQEIVGISIIGPRATELIGQGTV
HHHCCCEEECCCCCCCCCCEEEEECCCCEEEEEECCCHHHEEEEEEECCCHHHHHCCCEE
MIHTEVTADIMRDYIAAHPTLSEAIHEALLQAVGHAVHA
EEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLVVIGGGPAGYVAAITAAQNGKDVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIV
CEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHH
RKANHYGVILNNGSISIDWKQMQARKSQIVTQLVQGIQYLMKKNKIKVIQGKANFETDHR
HCCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCE
VRVIYGDKEDVVDGEQFIIAAGSEPTALPFAPFDGKWILNSSHAMSIDYIPKSLLIVGGG
EEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEECCC
VIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRGKLENDGVEIFTGAALKGLNN
EEEHHHHHHHHHHCCEEEEEECCHHHCCCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHH
YKKQASFEYEGSIEEVNPEFVLVSVGRKPRVQQLDLEKAGIQYSNKGISVNEHMQTNVSH
HHHHCCCEECCCHHHCCCCEEEEECCCCCCCCEECHHHHCCEECCCCCCCCHHHHCCCCE
IYAAGDVIGGIQLAHVAFHEGTTAALHASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDA
EEECCCHHCCEEEEEEEEECCCEEEEEECCCEEEEEEEECCEEEECCCCHHHCCCCCHHH
REKYGDIQIGEFPFTANGKALIIGEQTGKVKVIVEPKYQEIVGISIIGPRATELIGQGTV
HHHCCCEEECCCCCCCCCCEEEEECCCCEEEEEECCCHHHEEEEEEECCCHHHHHCCCEE
MIHTEVTADIMRDYIAAHPTLSEAIHEALLQAVGHAVHA
EEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377 [H]