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The map label for this gene is vanS [H]

Identifier: 222096096

GI number: 222096096

Start: 2398805

End: 2399962

Strand: Reverse

Name: vanS [H]

Synonym: BCQ_2436

Alternate gene names: 222096096

Gene position: 2399962-2398805 (Counterclockwise)

Preceding gene: 222096097

Following gene: 222096095

Centisome position: 46.03

GC content: 31.95

Gene sequence:

>1158_bases
TTGAGAAATGAAGCATTTGATATATTAAAAGATATCCCGAAATGGAAACTTATTTTTTGGCTCTTATTAGCTATCACACT
CACTCCTATTACTGAATTAATTATATATCGCCTCGTTACGAGTGTAATTGAGAGTTCGCTTACGTTAAGCGAACTTGGCA
AAGAATTCATTAGAATTTTTGGTTATGAAAAATATAAAGGAATAAAAGAATCTCTATGGATGATGATTGTCTCTTATTTA
TTTTTATTTTCTATTTTCTCATCTTACCTATACATTTTTTATCGTCATGAAAGAAAGCTTTACTATGAAACTTGTATAAA
AAAAATGATAGAAGAGATTCGCTATATTGCAAATGGAAACTTCAATCATAAAGTTTCTATCGTAAACCATAATTATCTAG
AAGAGTTAGCAAGCGGGGTTAATCAAATTGTTGAACAATTGAAAGTCTCGATTGATGAAGAAAGACAGGCAGAACAAGCA
AAAAGCGAACTGATTACAAATGTATCGCATGATTTACGTACTCCACTTACCTCTATAGTTGGATACGTGAATTTAATCCA
CCATGATAATTATAGAGATGAAGTAGAATTACGTCATTATATACAGGTCATTTACGATAAAGTAACTCGTCTTAACGCAT
TAATGAATGATTTGTTTGAATATACACGTGTTCAAAATAAAGAATTAAGTTTATATTCTGTCCCTATTGATATTGTGGAG
CTTCTTGGACAATTAACAGTTCAATTTCGAATACAGCTGCAAGAGGCTAATATTGATTGTCGTCCCTTTTTCCCATCAAA
GAAGCTAATGGTATTAGCTGATGGAGACAAATTAGTACGCGTATTTGAAAATCTAATTATTAATGCTATCGCTTACGGTA
ACGATGGTGATTATATTGATATTACTGCTTATGAAAGTAATAATATGATCGCAATCGATATAACCAATTACGGCCAACCT
ATTCCAAGTACCGATTTACCTCATATTTTTGAACGTTTTTATCGCGTTGAGAAATCGCGCTCTACAAACACTGGTGGATC
TGGACTCGGATTGGCAATTGCAAAAAGTATTGTCGAATTGCATAAAGGAACAATCGAAGTGTATAGTGATGACAAAAAAA
CAACATTCACTGTAAAACTACAACCATATAAATGTTAA

Upstream 100 bases:

>100_bases
AAAACTGTAATGGTCCACATTAGTAATTTACGGGACAAACTTGAAACAGGAATGAATGGAGAAAAATTTATTCATACTGT
TTGGGGAGTAGGCTATAAAA

Downstream 100 bases:

>100_bases
TACAGCTTAATTTTAGGTCCAATTTCTTACGATATAAGAAATTGGACCTTTTATTTTCCAGTAACATCCAAAATTTAATA
ATCCTTTATAATTCTTTAGA

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIESSLTLSELGKEFIRIFGYEKYKGIKESLWMMIVSYL
FLFSIFSSYLYIFYRHERKLYYETCIKKMIEEIRYIANGNFNHKVSIVNHNYLEELASGVNQIVEQLKVSIDEERQAEQA
KSELITNVSHDLRTPLTSIVGYVNLIHHDNYRDEVELRHYIQVIYDKVTRLNALMNDLFEYTRVQNKELSLYSVPIDIVE
LLGQLTVQFRIQLQEANIDCRPFFPSKKLMVLADGDKLVRVFENLIINAIAYGNDGDYIDITAYESNNMIAIDITNYGQP
IPSTDLPHIFERFYRVEKSRSTNTGGSGLGLAIAKSIVELHKGTIEVYSDDKKTTFTVKLQPYKC

Sequences:

>Translated_385_residues
MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIESSLTLSELGKEFIRIFGYEKYKGIKESLWMMIVSYL
FLFSIFSSYLYIFYRHERKLYYETCIKKMIEEIRYIANGNFNHKVSIVNHNYLEELASGVNQIVEQLKVSIDEERQAEQA
KSELITNVSHDLRTPLTSIVGYVNLIHHDNYRDEVELRHYIQVIYDKVTRLNALMNDLFEYTRVQNKELSLYSVPIDIVE
LLGQLTVQFRIQLQEANIDCRPFFPSKKLMVLADGDKLVRVFENLIINAIAYGNDGDYIDITAYESNNMIAIDITNYGQP
IPSTDLPHIFERFYRVEKSRSTNTGGSGLGLAIAKSIVELHKGTIEVYSDDKKTTFTVKLQPYKC
>Mature_385_residues
MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIESSLTLSELGKEFIRIFGYEKYKGIKESLWMMIVSYL
FLFSIFSSYLYIFYRHERKLYYETCIKKMIEEIRYIANGNFNHKVSIVNHNYLEELASGVNQIVEQLKVSIDEERQAEQA
KSELITNVSHDLRTPLTSIVGYVNLIHHDNYRDEVELRHYIQVIYDKVTRLNALMNDLFEYTRVQNKELSLYSVPIDIVE
LLGQLTVQFRIQLQEANIDCRPFFPSKKLMVLADGDKLVRVFENLIINAIAYGNDGDYIDITAYESNNMIAIDITNYGQP
IPSTDLPHIFERFYRVEKSRSTNTGGSGLGLAIAKSIVELHKGTIEVYSDDKKTTFTVKLQPYKC

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=276, Percent_Identity=27.8985507246377, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1786783, Length=248, Percent_Identity=28.6290322580645, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=28, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI48994928, Length=244, Percent_Identity=27.0491803278689, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=32.7731092436975, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693157, Length=307, Percent_Identity=22.8013029315961, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786912, Length=271, Percent_Identity=26.9372693726937, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789149, Length=297, Percent_Identity=26.2626262626263, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790346, Length=264, Percent_Identity=25.3787878787879, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87081816, Length=276, Percent_Identity=25.7246376811594, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1787894, Length=271, Percent_Identity=26.5682656826568, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788549, Length=262, Percent_Identity=27.8625954198473, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082128, Length=216, Percent_Identity=26.3888888888889, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788713, Length=246, Percent_Identity=23.9837398373984, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789808, Length=265, Percent_Identity=24.5283018867925, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=224, Percent_Identity=25, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788279, Length=227, Percent_Identity=26.8722466960352, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790300, Length=254, Percent_Identity=28.740157480315, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44814; Mature: 44814

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIESSLTLSELGKEFIRIF
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GYEKYKGIKESLWMMIVSYLFLFSIFSSYLYIFYRHERKLYYETCIKKMIEEIRYIANGN
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCC
FNHKVSIVNHNYLEELASGVNQIVEQLKVSIDEERQAEQAKSELITNVSHDLRTPLTSIV
CCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVNLIHHDNYRDEVELRHYIQVIYDKVTRLNALMNDLFEYTRVQNKELSLYSVPIDIVE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHH
LLGQLTVQFRIQLQEANIDCRPFFPSKKLMVLADGDKLVRVFENLIINAIAYGNDGDYID
HHHHHEEHHEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHEECCCCCEEE
ITAYESNNMIAIDITNYGQPIPSTDLPHIFERFYRVEKSRSTNTGGSGLGLAIAKSIVEL
EEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
HKGTIEVYSDDKKTTFTVKLQPYKC
HCCEEEEEECCCCEEEEEEEECCCC
>Mature Secondary Structure
MRNEAFDILKDIPKWKLIFWLLLAITLTPITELIIYRLVTSVIESSLTLSELGKEFIRIF
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GYEKYKGIKESLWMMIVSYLFLFSIFSSYLYIFYRHERKLYYETCIKKMIEEIRYIANGN
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCC
FNHKVSIVNHNYLEELASGVNQIVEQLKVSIDEERQAEQAKSELITNVSHDLRTPLTSIV
CCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYVNLIHHDNYRDEVELRHYIQVIYDKVTRLNALMNDLFEYTRVQNKELSLYSVPIDIVE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHH
LLGQLTVQFRIQLQEANIDCRPFFPSKKLMVLADGDKLVRVFENLIINAIAYGNDGDYID
HHHHHEEHHEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHEECCCCCEEE
ITAYESNNMIAIDITNYGQPIPSTDLPHIFERFYRVEKSRSTNTGGSGLGLAIAKSIVEL
EEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
HKGTIEVYSDDKKTTFTVKLQPYKC
HCCEEEEEECCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]