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The map label for this gene is dacA [H]

Identifier: 222096095

GI number: 222096095

Start: 2397607

End: 2398623

Strand: Reverse

Name: dacA [H]

Synonym: BCQ_2435

Alternate gene names: 222096095

Gene position: 2398623-2397607 (Counterclockwise)

Preceding gene: 222096096

Following gene: 222096083

Centisome position: 46.0

GC content: 31.56

Gene sequence:

>1017_bases
ATGCAATTCTTAAAAAAATTCACAATTTTATTATTATCTTTTGTTATTACTGCACTTATCGTTTTATATTTTTATCTTTA
TGTAAATGGCCCAATTATTCAAGCTAAATCAGCTATTTTAATAGATGCTCATTCAGGTGAAGTTGTTTATACAAAAAATG
AAGAAACTCCTATACAATCGGCTACTTTATCTAAATTAATGACTGAATATATCACACTGGAACAACTAAATGAAGGAACT
ATACAGTTAGATGAAGTAGTAAAAATAAGTAACGAAGTTTTCCGAGCAGAAACGAGTCCTATACAAGTAACTTCGAAAGA
TAAAACGACGGTTCGTGATTTACTTCATACACTGTTATTGACAGGAAATAATCGTTCCGCACTTGCTCTTGCAGAACACA
TCGCTGGAAACGAAGATAAATTCACACAGTTAATGAATGAAAAAGCAAAACACCTAAAGTTATCACAATCATCTCCGTTT
CTAAACGCTACTGGAATGAATAACGATACAAATAAACTTTCAACTACAACAGCAATTGAAGTTGCTAAACTAGCAACACA
ATTAGTAACAGACTTTCCAGATGTACTGAACGTTACAAAACTAACCTCTTACCAGTTCACCTTCAAAGATACGAAAGTTT
TCAACACCAATAAAATGATATATTCCCTTAATAAAAACATTAAACACCAAGGTGTTGATGGCTTACAAACTAGTTTTTCA
ACTAATGGTAATTATAGTTTTGTCAGTACAGCAAAACGAGGAGATACTAGACTTATTTCAGTAATATTAGACGCCGATAA
TGAAAACAGCACTTTTATTGAAACTAAAAAATTACTACAGTACGGCTTTGACCCCTCCTCGTACTCTGCACTCCAAGCAT
TTAAAGATGCTGTTACATCTTGGACAGTTTTGCTTCAGTTTAAAAATTTGATCATACAAACATTGTTGATTTTCTTTATT
ATTACAATTTTAATGTTCTTACATATACGTCAAAAAAAATCCGAGGATTTCAACTAG

Upstream 100 bases:

>100_bases
TCCTTTATAATTCTTTAGAAATTCTTTACCTTTTCTTTCTTCCTAATTAAGCATTCCTTTTTACAATATTATTATCGATG
TCATAATAGGAGGATCGCTT

Downstream 100 bases:

>100_bases
GAAATCTTCTTATATATAAAAAACGGCTTACATTTACAATGCAAGCCGTTTTCTAAATCTATAGTCCTCTCCCAATTAAA
TTCGCTGAAACAGGCTGAAG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQFLKKFTILLLSFVITALIVLYFYLYVNGPIIQAKSAILIDAHSGEVVYTKNEETPIQSATLSKLMTEYITLEQLNEGT
IQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHTLLLTGNNRSALALAEHIAGNEDKFTQLMNEKAKHLKLSQSSPF
LNATGMNNDTNKLSTTTAIEVAKLATQLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKHQGVDGLQTSFS
TNGNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWTVLLQFKNLIIQTLLIFFI
ITILMFLHIRQKKSEDFN

Sequences:

>Translated_338_residues
MQFLKKFTILLLSFVITALIVLYFYLYVNGPIIQAKSAILIDAHSGEVVYTKNEETPIQSATLSKLMTEYITLEQLNEGT
IQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHTLLLTGNNRSALALAEHIAGNEDKFTQLMNEKAKHLKLSQSSPF
LNATGMNNDTNKLSTTTAIEVAKLATQLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKHQGVDGLQTSFS
TNGNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWTVLLQFKNLIIQTLLIFFI
ITILMFLHIRQKKSEDFN
>Mature_338_residues
MQFLKKFTILLLSFVITALIVLYFYLYVNGPIIQAKSAILIDAHSGEVVYTKNEETPIQSATLSKLMTEYITLEQLNEGT
IQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHTLLLTGNNRSALALAEHIAGNEDKFTQLMNEKAKHLKLSQSSPF
LNATGMNNDTNKLSTTTAIEVAKLATQLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKHQGVDGLQTSFS
TNGNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTSWTVLLQFKNLIIQTLLIFFI
ITILMFLHIRQKKSEDFN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=262, Percent_Identity=30.5343511450382, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87082022, Length=259, Percent_Identity=27.4131274131274, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1786851, Length=262, Percent_Identity=29.0076335877863, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87082054, Length=259, Percent_Identity=25.0965250965251, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 38072; Mature: 38072

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLKKFTILLLSFVITALIVLYFYLYVNGPIIQAKSAILIDAHSGEVVYTKNEETPIQS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEECCCCCCHHH
ATLSKLMTEYITLEQLNEGTIQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHTLLL
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHH
TGNNRSALALAEHIAGNEDKFTQLMNEKAKHLKLSQSSPFLNATGMNNDTNKLSTTTAIE
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCEEECCCCCCCCCHHHHHHHH
VAKLATQLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKHQGVDGLQTSFS
HHHHHHHHHHCCHHHHHHHHHEEEEEEECCCEEEECCCEEEEECCCCCCCCCCHHHHCCC
TNGNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTS
CCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
WTVLLQFKNLIIQTLLIFFIITILMFLHIRQKKSEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQFLKKFTILLLSFVITALIVLYFYLYVNGPIIQAKSAILIDAHSGEVVYTKNEETPIQS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCEEEECCCCCCHHH
ATLSKLMTEYITLEQLNEGTIQLDEVVKISNEVFRAETSPIQVTSKDKTTVRDLLHTLLL
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHH
TGNNRSALALAEHIAGNEDKFTQLMNEKAKHLKLSQSSPFLNATGMNNDTNKLSTTTAIE
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCEEECCCCCCCCCHHHHHHHH
VAKLATQLVTDFPDVLNVTKLTSYQFTFKDTKVFNTNKMIYSLNKNIKHQGVDGLQTSFS
HHHHHHHHHHCCHHHHHHHHHEEEEEEECCCEEEECCCEEEEECCCCCCCCCCHHHHCCC
TNGNYSFVSTAKRGDTRLISVILDADNENSTFIETKKLLQYGFDPSSYSALQAFKDAVTS
CCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
WTVLLQFKNLIIQTLLIFFIITILMFLHIRQKKSEDFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]