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The map label for this gene is ypcP [H]

Identifier: 222095307

GI number: 222095307

Start: 1614388

End: 1615254

Strand: Direct

Name: ypcP [H]

Synonym: BCQ_1647

Alternate gene names: 222095307

Gene position: 1614388-1615254 (Clockwise)

Preceding gene: 222095306

Following gene: 222095308

Centisome position: 30.96

GC content: 36.79

Gene sequence:

>867_bases
ATGAAAAAAGTATTATTAGTTGATGGTATGGCACTATTATTTCGTGCTTTTTATGCAACAAGTGTCTACGGGCAGTTTAT
GAAACGACAAGATGGTACCCCTACAAACGGGATTCATGGTTATATGAAACATTTATTAACGGCAATGCAAGCAATTGAAC
CGACACACATCGTAACATGCTGGGATATGGGAAGTACAACATTTAGAACAGAGTCGTTCTCAAATTATAAAGCGAATCGT
GCAGCGCCACCTGAAGAATTAATTCCGCAATTTGATCTAGTGCAAGAAATGACTGTAAAATTATCTGTTCCAGTTATCGG
CATGAAAGGGTATGAAGCGGATGATTGTATCGGTACACTAGCAAAACAATACTGCAATGAAGCGGAAGTTTATATTTTAA
CAGGTGATACGGATTTACTTCAACTTGTTGATAAGAACGTTACAGTTATGCTTCTGCGCAAAGGAATTGGAAATTACGAG
TATTACACACCGGAGAAAATTATGGAAGAAAAAGGTGTAGAACCGTGGCAAATCGTGCATGCGAAAGCTTTCATGGGAGA
TACGAGCGATAATTACCCAGGTGTAAAAGGTATCGGTGAAAAAACAGCGTATAAGCTTATTCAAGAACATGGTACAGTAG
CAACTGTACTAGAAAATGTGGCAGCATTAACGAAAGCGCAACGTACGAAGATCGAAAGTGATTTAGAGAATTTAAATATT
TCATTACAATTAGCGCAAATTCATTGTGAAGTTCCTATTTCATGTTCACTAGAAGAAGGATTACACACAATAGATGAAGA
AAAATTACGATTCGTTTGTGAAGAAATGAATTGGGGAAGACCTGAAATATTAATCAATATGCTATAA

Upstream 100 bases:

>100_bases
ATGTATAATAGACAAAGTTGAAAAACGTAGAATGGTAATCTATTTATATAACCTTACGTGATGACGTGGGGTTATTATTT
TTTTGGGTAGGAGAGGAATT

Downstream 100 bases:

>100_bases
GTACTAGGAAGACCGGTTAAAAGTAAAATGAATCGGTCTTCCTTTTTTGTTCACAAAGTTGTCAGAAAATAAACGTAATT
TTCATGTAAAGTTCAAATGG

Product: 5'-3' exonuclease

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKKVLLVDGMALLFRAFYATSVYGQFMKRQDGTPTNGIHGYMKHLLTAMQAIEPTHIVTCWDMGSTTFRTESFSNYKANR
AAPPEELIPQFDLVQEMTVKLSVPVIGMKGYEADDCIGTLAKQYCNEAEVYILTGDTDLLQLVDKNVTVMLLRKGIGNYE
YYTPEKIMEEKGVEPWQIVHAKAFMGDTSDNYPGVKGIGEKTAYKLIQEHGTVATVLENVAALTKAQRTKIESDLENLNI
SLQLAQIHCEVPISCSLEEGLHTIDEEKLRFVCEEMNWGRPEILINML

Sequences:

>Translated_288_residues
MKKVLLVDGMALLFRAFYATSVYGQFMKRQDGTPTNGIHGYMKHLLTAMQAIEPTHIVTCWDMGSTTFRTESFSNYKANR
AAPPEELIPQFDLVQEMTVKLSVPVIGMKGYEADDCIGTLAKQYCNEAEVYILTGDTDLLQLVDKNVTVMLLRKGIGNYE
YYTPEKIMEEKGVEPWQIVHAKAFMGDTSDNYPGVKGIGEKTAYKLIQEHGTVATVLENVAALTKAQRTKIESDLENLNI
SLQLAQIHCEVPISCSLEEGLHTIDEEKLRFVCEEMNWGRPEILINML
>Mature_288_residues
MKKVLLVDGMALLFRAFYATSVYGQFMKRQDGTPTNGIHGYMKHLLTAMQAIEPTHIVTCWDMGSTTFRTESFSNYKANR
AAPPEELIPQFDLVQEMTVKLSVPVIGMKGYEADDCIGTLAKQYCNEAEVYILTGDTDLLQLVDKNVTVMLLRKGIGNYE
YYTPEKIMEEKGVEPWQIVHAKAFMGDTSDNYPGVKGIGEKTAYKLIQEHGTVATVLENVAALTKAQRTKIESDLENLNI
SLQLAQIHCEVPISCSLEEGLHTIDEEKLRFVCEEMNWGRPEILINML

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=264, Percent_Identity=34.469696969697, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI226510970, Length=210, Percent_Identity=27.1428571428571, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 32398; Mature: 32398

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLLVDGMALLFRAFYATSVYGQFMKRQDGTPTNGIHGYMKHLLTAMQAIEPTHIVTC
CCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
WDMGSTTFRTESFSNYKANRAAPPEELIPQFDLVQEMTVKLSVPVIGMKGYEADDCIGTL
EECCCCEEECCCCCCCCCCCCCCHHHHCCHHHHHHHHHHEEECCEEECCCCCHHHHHHHH
AKQYCNEAEVYILTGDTDLLQLVDKNVTVMLLRKGIGNYEYYTPEKIMEEKGVEPWQIVH
HHHHCCCCEEEEEECCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCHHHHHH
AKAFMGDTSDNYPGVKGIGEKTAYKLIQEHGTVATVLENVAALTKAQRTKIESDLENLNI
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
SLQLAQIHCEVPISCSLEEGLHTIDEEKLRFVCEEMNWGRPEILINML
EEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MKKVLLVDGMALLFRAFYATSVYGQFMKRQDGTPTNGIHGYMKHLLTAMQAIEPTHIVTC
CCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
WDMGSTTFRTESFSNYKANRAAPPEELIPQFDLVQEMTVKLSVPVIGMKGYEADDCIGTL
EECCCCEEECCCCCCCCCCCCCCHHHHCCHHHHHHHHHHEEECCEEECCCCCHHHHHHHH
AKQYCNEAEVYILTGDTDLLQLVDKNVTVMLLRKGIGNYEYYTPEKIMEEKGVEPWQIVH
HHHHCCCCEEEEEECCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCHHHHHH
AKAFMGDTSDNYPGVKGIGEKTAYKLIQEHGTVATVLENVAALTKAQRTKIESDLENLNI
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
SLQLAQIHCEVPISCSLEEGLHTIDEEKLRFVCEEMNWGRPEILINML
EEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]