Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 222095135

GI number: 222095135

Start: 1450795

End: 1452189

Strand: Direct

Name: leuC [H]

Synonym: BCQ_1475

Alternate gene names: 222095135

Gene position: 1450795-1452189 (Clockwise)

Preceding gene: 222095134

Following gene: 222095136

Centisome position: 27.82

GC content: 41.36

Gene sequence:

>1395_bases
ATGGGGAAAAGGTTGCTAGATAAACTTTGGGAAAGACACGTAGTTGCGACAAATGAAAATGGATTAGATTTATTATATAT
CGATCTGCACCTTGTTCATGAAGTAACGTCACCGCAAGCCTTTGAAGGCTTGCGGCTTACAAATCGAACGGTACGCAGAC
CAGATTTAACATTCGCAACGATGGATCATAATATTCCAACGAAAGATGTTTGGAATATTACCGATCGCATTGCGAAGCAG
CAATTAGATACACTTCGGGAAAATTGTAAACAATTTCAGGTGCCGTTAGCAGATATCGGAGATGAAGAGCAAGGGATCGT
TCATGTTATCGGACCAGAACTTGGGCTCACGCAGCCAGGAAAAACAATTGTTTGTGGTGATAGTCATACAGCAACGCACG
GTGCGTTTGGCGCGCTAGCATTTGGTATTGGTACGAGTGAAGTGGAACATGTATTGGCAACGCAAACGTTATGGCAAAGA
AAACCGAAAGCGATGGGAATTGAGTTAAAAGGAAAATTACAGAAAGGCGTTTACGCAAAAGATATTATTTTGCATCTCCT
TTCAAAGTACGGTGTAGCAGTTGGAACTGGATACGTAATGGAATTTTACGGAGAGACGATTCAGGCTATGGAGATGGAAG
AGAGAATGACGCTTTGTAATATGGCGATTGAAGGCGGAGCAAAAGCGGGTATTATCGCGCCGGATGAAAAAACAGTTGCT
TATGTAAAAGGACGTAAATATGCACCGAGAGACTATGAAACTTTCGAGAAGAAATGGTTTGAACTTTATACAGATGCAGA
TGCAATTTATGATTTACATATTTCGATAGATGTTACAGATTTAGCGCCATACGTTACGTGGGGAACAAATCCGAGTATGG
GAGTTCGTATTGATGAGAAATTGCCAGAAAAACATGATGTAAATGACGAAAGAGCATTTTCTTATATGGGATTAAGCCCT
GGACAAAGTACGTATGATATTCCAGTTCAGCATGTTTTCATTGGATCTTGTACAAATTCCAGGCTATCTGATTTAGAAAT
TGCTGCAGCTGTTGTGAAAGGGAGAAAGGTAAAAGAAGGTGTGCGAGCACTCGTTGTACCTGGATCGAAAAGAGTAAGGG
ACGCGGCGATGCAAAAAGGACTACATCACATATTTGAAGAAGCAGGATTTGAATGGAGAGAACCTGGATGTTCCATGTGT
CTTGGAATGAATCCAGATCAAGTACCTGAAGGAGAGCATTGCGCTTCAACTTCAAATCGTAATTTTGAAGGAAGACAAGG
AAAAGGAGCACGAACGCATTTAGTTAGCCCAGCAATGGCGGCAGCAGCTGCGTTATATGGTCATTTTGTTGATACAAGAA
AGGAGAGTTATGATGGAGCCATTTCGTATTCATAA

Upstream 100 bases:

>100_bases
AATGTACAGCAGATATCGGGGGGACTGAAACGACAACTTCATTTACGAAGGCAGTAATGCAAGAAATGGAAGAGCAAGCG
CTAGTAGGGAGAGGAAGATA

Downstream 100 bases:

>100_bases
AGGTACTGCCGCAGTACTTATGAATAATAACATTGATACGGATCAAATTATTCCGAAGCAATATTTAAAGAGAATTGAAA
GAACTGGGTTTGGAAAGTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MGKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQ
QLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIQAMEMEERMTLCNMAIEGGAKAGIIAPDEKTVA
YVKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDVNDERAFSYMGLSP
GQSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEGVRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMC
LGMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS

Sequences:

>Translated_464_residues
MGKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQ
QLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIQAMEMEERMTLCNMAIEGGAKAGIIAPDEKTVA
YVKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDVNDERAFSYMGLSP
GQSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEGVRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMC
LGMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS
>Mature_463_residues
GKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQQ
LDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRK
PKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIQAMEMEERMTLCNMAIEGGAKAGIIAPDEKTVAY
VKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDVNDERAFSYMGLSPG
QSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEGVRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCL
GMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=355, Percent_Identity=26.7605633802817, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI8659555, Length=387, Percent_Identity=26.3565891472868, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=26.3586956521739, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=58.2795698924731, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1787531, Length=363, Percent_Identity=28.3746556473829, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI87081781, Length=358, Percent_Identity=24.8603351955307, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI2367097, Length=337, Percent_Identity=25.2225519287834, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=359, Percent_Identity=30.6406685236769, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=359, Percent_Identity=30.6406685236769, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=30.1639344262295, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=423, Percent_Identity=26.4775413711584, Blast_Score=104, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=55.9322033898305, Blast_Score=494, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320440, Length=414, Percent_Identity=28.5024154589372, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=356, Percent_Identity=28.9325842696629, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=404, Percent_Identity=28.960396039604, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI281365315, Length=393, Percent_Identity=26.972010178117, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17864292, Length=393, Percent_Identity=26.972010178117, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=27.0833333333333, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571643, Length=391, Percent_Identity=28.1329923273657, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24645686, Length=380, Percent_Identity=27.6315789473684, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17137564, Length=373, Percent_Identity=25.201072386059, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51426; Mature: 51295

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFAT
CCHHHHHHHHHCCEEEECCCCCEEEEEEEEEHHHHCCCHHHCCCCCCCCCCCCCCCEEEE
MDHNIPTKDVWNITDRIAKQQLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLQKGVYAK
CEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHH
DIILHLLSKYGVAVGTGYVMEFYGETIQAMEMEERMTLCNMAIEGGAKAGIIAPDEKTVA
HHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEK
EEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCCEEEECCCCCCCEEEHHH
LPEKHDVNDERAFSYMGLSPGQSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEG
CCCCCCCCHHHHEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHCC
VRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GKRLLDKLWERHVVATNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFAT
CHHHHHHHHHCCEEEECCCCCEEEEEEEEEHHHHCCCHHHCCCCCCCCCCCCCCCEEEE
MDHNIPTKDVWNITDRIAKQQLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLQKGVYAK
CEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHH
DIILHLLSKYGVAVGTGYVMEFYGETIQAMEMEERMTLCNMAIEGGAKAGIIAPDEKTVA
HHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEK
EEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCCEEEECCCCCCCEEEHHH
LPEKHDVNDERAFSYMGLSPGQSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEG
CCCCCCCCHHHHEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHCC
VRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA