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The map label for this gene is leuB [H]

Identifier: 222095134

GI number: 222095134

Start: 1449665

End: 1450795

Strand: Direct

Name: leuB [H]

Synonym: BCQ_1474

Alternate gene names: 222095134

Gene position: 1449665-1450795 (Clockwise)

Preceding gene: 222095133

Following gene: 222095135

Centisome position: 27.8

GC content: 41.03

Gene sequence:

>1131_bases
GTGAGAGGGGATATTCTCACCTATCAAATTAAAGTTCTGAAAATTCAGTTAAAAGGGGTGTTGAAATTGGAAAAACGTAT
CGTTTGTTTAGCTGGTGATGGGGTTGGACCGGAAGTTATGGAAAGTGCGAAGGAAGTATTGCATATGGTAGAAAGGTTAT
ATGGTCATCATTTTCATTTGCAAGATGAGTACTTTGGCGGGAGTGCTATCGATTTAAATGGGCAACCATTACCACAACGA
ACGCTTGCCGCTTGTTTAGCAAGTGATGCGGTTTTACTAGGAGCAGTTGGTGGACCAAGGTGGGATAGTGCGAAAGAAAG
ACCAGAGAAAGGATTATTAGCTTTAAGAAAAGGGCTCGGTGTATTTGCGAACGTTCGCCCTGTAACGGTAGAAAGTGCAA
CGGCACATTTATCGCCATTAAAAAAGGCTGATGAAATTGATTTCGTTGTCGTTCGTGAATTAACAGGTGGGATTTATTTT
TCTTATCCGAAAGAACGAACTGACGAAGTAGCAACGGATACACTTACGTATCATCGCCATGAAATTGAACGTATCGTTTC
TTATGCTTTTCAATTAGCGAGTAAACGGAAGAAAAAAGTAACATCTATTGATAAGGCGAATGTTTTAGAATCTAGTAAAC
TATGGAGAACTGTAACAGAAGAAGTAGCACTTCGTTATCCAGATGTTGAACTAGAACATATTTTAGTAGATGCAGCTGCT
ATGGAGTTAATTCGGAATCCAGGACGGTTTGATGTAATTGTAACAGAAAATTTATTTGGGGATATTTTAAGTGACGAAGC
TTCCGTATTAGCTGGATCATTAGGAATGCTTCCGTCAGCAAGTCACGCGGAAAAGGGTCCTTCATTATACGAGCCTATTC
ACGGATCGGCACCGGATATTGCCGGGAAAAATAAGGCGAATCCAATTGCGATGATGCGCTCTGTTGCCATGATGCTTGGA
CAATCATTCGGATTAACAAGAGAAGGGTGTGCAATTGAAGAAGCGATTTCAGCAGTCCTTAAATTAGGAAAATGTACAGC
AGATATCGGGGGGACTGAAACGACAACTTCATTTACGAAGGCAGTAATGCAAGAAATGGAAGAGCAAGCGCTAGTAGGGA
GAGGAAGATAA

Upstream 100 bases:

>100_bases
GTGTTGCGCAAGACGTATTGGAAGCGTCGGCGAGAGCATATGTCCACGCAGCGGGGAAATTGAAATCTTTTATCCAGCTT
GTGAAATAAGTGTTTGATAG

Downstream 100 bases:

>100_bases
TGGGGAAAAGGTTGCTAGATAAACTTTGGGAAAGACACGTAGTTGCGACAAATGAAAATGGATTAGATTTATTATATATC
GATCTGCACCTTGTTCATGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRGDILTYQIKVLKIQLKGVLKLEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEYFGGSAIDLNGQPLPQR
TLAACLASDAVLLGAVGGPRWDSAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYF
SYPKERTDEVATDTLTYHRHEIERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAA
MELIRNPGRFDVIVTENLFGDILSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLG
QSFGLTREGCAIEEAISAVLKLGKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR

Sequences:

>Translated_376_residues
MRGDILTYQIKVLKIQLKGVLKLEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEYFGGSAIDLNGQPLPQR
TLAACLASDAVLLGAVGGPRWDSAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYF
SYPKERTDEVATDTLTYHRHEIERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAA
MELIRNPGRFDVIVTENLFGDILSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLG
QSFGLTREGCAIEEAISAVLKLGKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR
>Mature_376_residues
MRGDILTYQIKVLKIQLKGVLKLEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEYFGGSAIDLNGQPLPQR
TLAACLASDAVLLGAVGGPRWDSAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYF
SYPKERTDEVATDTLTYHRHEIERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAA
MELIRNPGRFDVIVTENLFGDILSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLG
QSFGLTREGCAIEEAISAVLKLGKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=349, Percent_Identity=30.945558739255, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI28178821, Length=354, Percent_Identity=30.5084745762712, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28178816, Length=348, Percent_Identity=30.7471264367816, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4758582, Length=255, Percent_Identity=31.3725490196078, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=34.5360824742268, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI28178838, Length=207, Percent_Identity=32.8502415458937, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI87081683, Length=333, Percent_Identity=51.0510510510511, Blast_Score=319, Evalue=1e-88,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=35.3932584269663, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787381, Length=373, Percent_Identity=23.8605898123324, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=31.0734463276836, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17550882, Length=360, Percent_Identity=30.5555555555556, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=379, Percent_Identity=26.9129287598945, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=27.9538904899135, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=47.7653631284916, Blast_Score=289, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=36.6946778711485, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=346, Percent_Identity=33.5260115606936, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=29.4617563739377, Blast_Score=132, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24643268, Length=348, Percent_Identity=31.8965517241379, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24643270, Length=348, Percent_Identity=31.8965517241379, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=30.1136363636364, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=30.1136363636364, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24661184, Length=357, Percent_Identity=27.4509803921569, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078637, Length=345, Percent_Identity=27.536231884058, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI161078633, Length=345, Percent_Identity=27.536231884058, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24650122, Length=345, Percent_Identity=27.536231884058, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI161078635, Length=345, Percent_Identity=27.536231884058, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI161078639, Length=342, Percent_Identity=27.7777777777778, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20130355, Length=342, Percent_Identity=24.2690058479532, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 40983; Mature: 40983

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGDILTYQIKVLKIQLKGVLKLEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHL
CCCCEEEEEEEEEEEEHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEE
QDEYFGGSAIDLNGQPLPQRTLAACLASDAVLLGAVGGPRWDSAKERPEKGLLALRKGLG
CCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCHHCCCHHHHHHHHHCCC
VFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRH
CEECCCEEEECCHHHHHHHHCCCCCCCEEEEEEHHCCEEEECCCHHHHHHHHHHHHHHHH
EIERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAA
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
MELIRNPGRFDVIVTENLFGDILSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDI
HHHHHCCCCEEEEEECHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCC
AGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKLGKCTADIGGTETTTSFTK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHCCCCCCCHHHHHHH
AVMQEMEEQALVGRGR
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRGDILTYQIKVLKIQLKGVLKLEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHL
CCCCEEEEEEEEEEEEHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEE
QDEYFGGSAIDLNGQPLPQRTLAACLASDAVLLGAVGGPRWDSAKERPEKGLLALRKGLG
CCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCHHCCCHHHHHHHHHCCC
VFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRH
CEECCCEEEECCHHHHHHHHCCCCCCCEEEEEEHHCCEEEECCCHHHHHHHHHHHHHHHH
EIERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAA
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
MELIRNPGRFDVIVTENLFGDILSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDI
HHHHHCCCCEEEEEECHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCC
AGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKLGKCTADIGGTETTTSFTK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHCCCCCCCHHHHHHH
AVMQEMEEQALVGRGR
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]