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The map label for this gene is sfcA [H]

Identifier: 222094331

GI number: 222094331

Start: 636156

End: 637355

Strand: Direct

Name: sfcA [H]

Synonym: BCQ_0645

Alternate gene names: 222094331

Gene position: 636156-637355 (Clockwise)

Preceding gene: 222094330

Following gene: 222094334

Centisome position: 12.2

GC content: 38.92

Gene sequence:

>1200_bases
ATGTTAGAGAATCAAATTAATGAAAGATCATTGTTATTGCATAAAGAATTAGTAGGGAAAATTGAAATTACAAGTAAAGT
AGAAGTAAATTCAGCGGATGATTTAAGCCTGACGTATACACCAGGTGTAGCGGAGTCTTGTAAAGCAATTGCGGCAGATG
AAGAAACGGTGTATGACTATACAGCACGTGGTAATATGGTGGCAGTTGTCTCAGATGGAACAGCGGTACTCGGTTTAGGA
AACATTGGACCGAAAGCAGCTATGCCTGTTATGGAAGGAAAAAGTATTTTGTTTAAGAAGTTTGCGAATGTAGATGCTTT
CCCATTATGTTTAGGCACAACGGATGTAGATGAAATCGTTACCCTTGTAAAAAATTTAGAGCCTACATTTGCAGGGATTA
ATTTAGAAGATATTGCAGCACCGCGTTGTTTTGAAATTGAAAAACGATTAAAAGAAGAAACGAATATTCCTGTATTCCAT
GATGATCAACATGGAACAGCTATTGTCGTTTTAGCAGCTGTTATTAATGCATTAAAAGTTGTTAGCAAACAAATGGATAA
TGTGAAAATCGTTATTAACGGTGCGGGTTCGGCAGGAATTGCAATTGGAAAATTATTATTAAAGGCTGGTGCAAAGCACA
TTACGCTAGTTAGCCTAGAAGGTATTGTTTGTGAAGGTGAAACATGGATGAACGAAGCACAAATTGAGGTTTCAAAGAAG
ACAAATCGAGAACATGTGCGTGGAACGTTAAAAGAAGCAATTCATCAAGCTGATATCTTTATCGGTGTATCTGCTCCTAA
CGTATTAACGAAAGAACTTGTACAGACGATGAATGAGAAAGCAATTGTGTTCGCTATGGCGAATCCAATTCCAGAAATTT
TCCCTGAGGATGCATTAGAGGCTGGGGCAGCTGTTGTTGGAACTGGGCGTTCGGATTATGCGAATCAAGTAAATAATGTA
TTGGCGTTTCCAGGAATATTCCGCGGTGCGTTAGATGTGCGCGCGACTGATATTACAGAAGAAATGAAATTAGCGGCAGC
ATATGGGATCGCTAACATCATTACGGACGAAGAACGTAATGCGAATTATGTAATCCCGAATCCATTAGATAAAAGAGTTG
TTCCAAGTGTTGCTGAAGCTGTGGCGAAGGCAGCTATTGATTCAGGCGTGACGCAAATTACGAAAATACCAAGTTATTAA

Upstream 100 bases:

>100_bases
TGGTTGTAACGGCGACAATATTATTAAAAATGTTTTCATGATCAAACAAGCTAGCTATATTCATATAGCTAGCTTGTCAA
ATTAAGGGGAGATTATAAGT

Downstream 100 bases:

>100_bases
TAGCTAAAAGCAGTGCCTAAAAAGGCACTGCTTTTTTGAGATAAGAAATCAAAATAATGGAGAAGTGTAACATTATTTAT
GTTTCCTTATTATTTATTTA

Product: malate dehydrogenase (malic enzyme) (nad-malic enzyme)

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MLENQINERSLLLHKELVGKIEITSKVEVNSADDLSLTYTPGVAESCKAIAADEETVYDYTARGNMVAVVSDGTAVLGLG
NIGPKAAMPVMEGKSILFKKFANVDAFPLCLGTTDVDEIVTLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFH
DDQHGTAIVVLAAVINALKVVSKQMDNVKIVINGAGSAGIAIGKLLLKAGAKHITLVSLEGIVCEGETWMNEAQIEVSKK
TNREHVRGTLKEAIHQADIFIGVSAPNVLTKELVQTMNEKAIVFAMANPIPEIFPEDALEAGAAVVGTGRSDYANQVNNV
LAFPGIFRGALDVRATDITEEMKLAAAYGIANIITDEERNANYVIPNPLDKRVVPSVAEAVAKAAIDSGVTQITKIPSY

Sequences:

>Translated_399_residues
MLENQINERSLLLHKELVGKIEITSKVEVNSADDLSLTYTPGVAESCKAIAADEETVYDYTARGNMVAVVSDGTAVLGLG
NIGPKAAMPVMEGKSILFKKFANVDAFPLCLGTTDVDEIVTLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFH
DDQHGTAIVVLAAVINALKVVSKQMDNVKIVINGAGSAGIAIGKLLLKAGAKHITLVSLEGIVCEGETWMNEAQIEVSKK
TNREHVRGTLKEAIHQADIFIGVSAPNVLTKELVQTMNEKAIVFAMANPIPEIFPEDALEAGAAVVGTGRSDYANQVNNV
LAFPGIFRGALDVRATDITEEMKLAAAYGIANIITDEERNANYVIPNPLDKRVVPSVAEAVAKAAIDSGVTQITKIPSY
>Mature_399_residues
MLENQINERSLLLHKELVGKIEITSKVEVNSADDLSLTYTPGVAESCKAIAADEETVYDYTARGNMVAVVSDGTAVLGLG
NIGPKAAMPVMEGKSILFKKFANVDAFPLCLGTTDVDEIVTLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFH
DDQHGTAIVVLAAVINALKVVSKQMDNVKIVINGAGSAGIAIGKLLLKAGAKHITLVSLEGIVCEGETWMNEAQIEVSKK
TNREHVRGTLKEAIHQADIFIGVSAPNVLTKELVQTMNEKAIVFAMANPIPEIFPEDALEAGAAVVGTGRSDYANQVNNV
LAFPGIFRGALDVRATDITEEMKLAAAYGIANIITDEERNANYVIPNPLDKRVVPSVAEAVAKAAIDSGVTQITKIPSY

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=449, Percent_Identity=23.8307349665924, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI4505145, Length=359, Percent_Identity=25.0696378830084, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI62420882, Length=422, Percent_Identity=22.9857819905213, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI62420880, Length=422, Percent_Identity=22.9857819905213, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI239049447, Length=422, Percent_Identity=22.9857819905213, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI270265879, Length=318, Percent_Identity=25.4716981132075, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788806, Length=407, Percent_Identity=48.4029484029484, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI87081919, Length=445, Percent_Identity=25.8426966292135, Blast_Score=88, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6322823, Length=269, Percent_Identity=26.7657992565056, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI78707240, Length=446, Percent_Identity=26.0089686098655, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI78707242, Length=439, Percent_Identity=25.9681093394077, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI78707238, Length=439, Percent_Identity=25.9681093394077, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281362672, Length=450, Percent_Identity=24.8888888888889, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281362674, Length=450, Percent_Identity=24.8888888888889, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356279, Length=450, Percent_Identity=24.8888888888889, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24646388, Length=448, Percent_Identity=23.6607142857143, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24646386, Length=448, Percent_Identity=23.6607142857143, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281363503, Length=404, Percent_Identity=23.019801980198, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281363505, Length=404, Percent_Identity=23.019801980198, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI78707236, Length=404, Percent_Identity=23.019801980198, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI78707232, Length=396, Percent_Identity=23.4848484848485, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 42689; Mature: 42689

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENQINERSLLLHKELVGKIEITSKVEVNSADDLSLTYTPGVAESCKAIAADEETVYDY
CCCCCCCHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCEEEE
TARGNMVAVVSDGTAVLGLGNIGPKAAMPVMEGKSILFKKFANVDAFPLCLGTTDVDEIV
ECCCCEEEEEECCEEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCCEEEEECCCCHHHHH
TLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFHDDQHGTAIVVLAAVINALKV
HHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
VSKQMDNVKIVINGAGSAGIAIGKLLLKAGAKHITLVSLEGIVCEGETWMNEAQIEVSKK
HHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCEEECCHHHCCCEEEEEECC
TNREHVRGTLKEAIHQADIFIGVSAPNVLTKELVQTMNEKAIVFAMANPIPEIFPEDALE
CCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCHHCCCHHHHH
AGAAVVGTGRSDYANQVNNVLAFPGIFRGALDVRATDITEEMKLAAAYGIANIITDEERN
CCCEEEECCCHHHHHHHHCEEECCHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCCCCCCC
ANYVIPNPLDKRVVPSVAEAVAKAAIDSGVTQITKIPSY
CCEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure
MLENQINERSLLLHKELVGKIEITSKVEVNSADDLSLTYTPGVAESCKAIAADEETVYDY
CCCCCCCHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCEEEE
TARGNMVAVVSDGTAVLGLGNIGPKAAMPVMEGKSILFKKFANVDAFPLCLGTTDVDEIV
ECCCCEEEEEECCEEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCCEEEEECCCCHHHHH
TLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFHDDQHGTAIVVLAAVINALKV
HHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
VSKQMDNVKIVINGAGSAGIAIGKLLLKAGAKHITLVSLEGIVCEGETWMNEAQIEVSKK
HHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCEEECCHHHCCCEEEEEECC
TNREHVRGTLKEAIHQADIFIGVSAPNVLTKELVQTMNEKAIVFAMANPIPEIFPEDALE
CCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCHHCCCHHHHH
AGAAVVGTGRSDYANQVNNVLAFPGIFRGALDVRATDITEEMKLAAAYGIANIITDEERN
CCCEEEECCCHHHHHHHHCEEECCHHHHHHCCEEEHHHHHHHHHHHHHHHHHHCCCCCCC
ANYVIPNPLDKRVVPSVAEAVAKAAIDSGVTQITKIPSY
CCEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]