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The map label for this gene is citS [H]

Identifier: 222094310

GI number: 222094310

Start: 613083

End: 614693

Strand: Direct

Name: citS [H]

Synonym: BCQ_0624

Alternate gene names: 222094310

Gene position: 613083-614693 (Clockwise)

Preceding gene: 222094309

Following gene: 222094311

Centisome position: 11.76

GC content: 34.57

Gene sequence:

>1611_bases
ATGGTTTTGAAATTTAAAAAATTAAAACTCCAACCGAGAATCACATTAACTATTAGTACACTTATTCTTGTTGTACTTAT
GCTAACGAGTTATTTATTTTATTACATATTATCTGAAACGGTAGAAGAGCAAATTGGCAAAAGAGCTTTGCATGTTGCGA
AAACAGTTGCTGCTATACCAGAAATCAAGGAAGCTTTTCAAACAGAAAATCCAGCTTCTATTATTCAACCGATTGCGGAA
AAAATTCGAATTGATACAGATGCTGATTTTATTGTAGTTGGAAATAAAGAAGGGATTCGTTATGCACATCCTAGGAAAGA
TAAAATAGGAGAAGCAATGGTTGGCGGAGATAACAAAGGAGTTTTATTAGAAGGGAAATCTTACGTTTCGAAAGCAACTG
GATCATTAGGCCCTTCATTACGCGGAAAAGTTCCAATCCATAATCAGGAAAATGAAATCATTGGTGTGGTATCTGTTGGA
TTTTCAATGGATGATATTCATGGAGCGGTAGAAGTGTATGGAAAGCGTGTGTTTTGGATTACAATAATAGGTCTATTAAT
TGGAGTAATAGGCTCTATATATTTAGCTGGCAGTATAAAAAGAATGATGTTTGGGATGGAGCCAGAAGAAATTTCATCTT
TATATGAAGAGCATAGTACAGTGATTCAATCTGTACGTGAGGGGATTATTGTCATTGATCAACATGGCATAATTAGTTTA
GTTAATCAAGCAGCCTATGATATTCTTTCGCTAGATGAACAACGAAATATTATTGGAGAATTTATTTTAGATATAATTCC
TAATTCAACGATATTAGATGTACTTCAAACTGGTGAAGAACAGTTTGACCGCCAATTAAATATAAAAGGGCAGGCTGTTA
TTGCGAACCGTCTACCCATTAAAGTAAAGAATAAAGTAACTGGCGTTGTATCGAGCTTACGTTTGAAATCTGAAATGGAT
CAGTTAACAGCAGAATTATCTCAGACGAAGCAATATACAGAGGCATTACGAGCGCAAACGCATGAATATAACAATTTGTT
GTATACGCTTTCAGGTCTTATCCAGCTAGAATTATATGAAGATGCTTTGGAGCTTATTCATAAGGAAACAGCGGTATATC
AAGATTTTGTTCAATTTATTATGAAACGAATTCAAAATCCATGGTTAGGAGGGATTTTAATTGGATTCTATAATAGAGCA
AGAGAGTTGAAGATTGAGTTTATGCTAGATAGAGAGAGTAGCTTAGACAAATTAAGCCCGCATATTGAGAGTAATTATGT
CGTTTCTATTTTAGGAAATTTAATTACGAATGCATTTGAAGCGATTGAAAGAAATGAAGAACATGATAAGAAAGTAAGAA
TGTTTGTAACTGATATCGGAGAAGAAATAGTCATTGAAGTGGAAGACTCAGGACAAGGGATTCATGATGAGGTCATTGCT
AGCATATTCTATAAAGGCTTCTCGACGAAAGAAGGCGAGAAGCGAGGATATGGATTAGCAAAGGTGAAAGAGTTAGTAGA
AGATTTAAATGGAAGTATCGCAATCGAAAAAGGGGATTTAGGTGGTGCATTGTTTATTATTGCATTACCAAAAGAGAGAG
GCGAATTGTAA

Upstream 100 bases:

>100_bases
GAAGAGAAAAAGAAAGAAATATAGAGTAACCGTGTTATGATTAAGTATAATTAAAGAGACTGTATATTCTTTAAATTCTA
TCTTTAATCATGTTAGGGGA

Downstream 100 bases:

>100_bases
TGGGTGAGGAGATTGAAGTATTGATTGTAGAAGATGATATTCGAATTGCAGACATTCATCGTCGTTTTACTGAAAAGATT
GAGGGATTTAAAGTAATTGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MVLKFKKLKLQPRITLTISTLILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQTENPASIIQPIAE
KIRIDTDADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIHNQENEIIGVVSVG
FSMDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQHGIISL
VNQAAYDILSLDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMD
QLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRA
RELKIEFMLDRESSLDKLSPHIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGIHDEVIA
SIFYKGFSTKEGEKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGEL

Sequences:

>Translated_536_residues
MVLKFKKLKLQPRITLTISTLILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQTENPASIIQPIAE
KIRIDTDADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIHNQENEIIGVVSVG
FSMDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQHGIISL
VNQAAYDILSLDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMD
QLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRA
RELKIEFMLDRESSLDKLSPHIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGIHDEVIA
SIFYKGFSTKEGEKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGEL
>Mature_536_residues
MVLKFKKLKLQPRITLTISTLILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQTENPASIIQPIAE
KIRIDTDADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIHNQENEIIGVVSVG
FSMDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQHGIISL
VNQAAYDILSLDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMD
QLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRA
RELKIEFMLDRESSLDKLSPHIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGIHDEVIA
SIFYKGFSTKEGEKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGEL

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=535, Percent_Identity=32.5233644859813, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1790567, Length=520, Percent_Identity=32.1153846153846, Blast_Score=280, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59906; Mature: 59906

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLKFKKLKLQPRITLTISTLILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIP
CCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIKEAFQTENPASIIQPIAEKIRIDTDADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKG
HHHHHHCCCCCHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCHHCCCHHCCCCCCC
VLLEGKSYVSKATGSLGPSLRGKVPIHNQENEIIGVVSVGFSMDDIHGAVEVYGKRVFWI
EEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHH
TIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQHGIISL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHH
VNQAAYDILSLDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPI
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCE
KVKNKVTGVVSSLRLKSEMDQLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYE
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARELKIEFMLDRESSLDKLSP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHEEEEEEEECCCCCHHHCCC
HIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGIHDEVIA
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
SIFYKGFSTKEGEKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGEL
HHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MVLKFKKLKLQPRITLTISTLILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIP
CCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIKEAFQTENPASIIQPIAEKIRIDTDADFIVVGNKEGIRYAHPRKDKIGEAMVGGDNKG
HHHHHHCCCCCHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCCCHHCCCHHCCCCCCC
VLLEGKSYVSKATGSLGPSLRGKVPIHNQENEIIGVVSVGFSMDDIHGAVEVYGKRVFWI
EEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHH
TIIGLLIGVIGSIYLAGSIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDQHGIISL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHH
VNQAAYDILSLDEQRNIIGEFILDIIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPI
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCE
KVKNKVTGVVSSLRLKSEMDQLTAELSQTKQYTEALRAQTHEYNNLLYTLSGLIQLELYE
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARELKIEFMLDRESSLDKLSP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHEEEEEEEECCCCCHHHCCC
HIESNYVVSILGNLITNAFEAIERNEEHDKKVRMFVTDIGEEIVIEVEDSGQGIHDEVIA
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
SIFYKGFSTKEGEKRGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGEL
HHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]