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The map label for this gene is dus2 [H]

Identifier: 222094091

GI number: 222094091

Start: 364253

End: 365242

Strand: Direct

Name: dus2 [H]

Synonym: BCQ_0384

Alternate gene names: 222094091

Gene position: 364253-365242 (Clockwise)

Preceding gene: 222094090

Following gene: 222094092

Centisome position: 6.99

GC content: 40.61

Gene sequence:

>990_bases
ATGATAGATAATTTTTGGCGTGATTTACCACGACCATTTTTCGTACTTGCACCAATGGAAGATGTGACAGACGTTGTTTT
CCGTCACGTAGTAAGTGAAGCTGGTCGTCCAGATGTATTCTTCACAGAGTTCACAAACTCAGATAGCTATTGTCATCCAG
AAGGTATGAAAAGCGTACGTGGTCGTTTAATTTTTACAGAAGATGAACAGCCAATGGTGGCACATATTTGGGGAGATAAT
CCTGAATATTTCCGTGAAATGAGTATTGGTATGGCAGAGTTAGGATTTAAAGGCATCGATATTAATATGGGTTGCCCTGT
GCCAAACGTAGCATCAAGAGGAAAAGGTAGTGGCCTTATCTTGCGTCCAGACGTTGCGGCAGAACTTATTCAAGCTGCAA
AAGCAGGCGGACTACCTGTCAGCGTAAAAACAAGACTTGGCTTTAAAGAGTTAAGCGAGTGGGAAGATTGGTTAACGCAC
ATTTTCAAACAAGATATTGCAAACCTTTCTATTCACTTACGTACAAGAGAAGAGATGAGCCAAGTAGATGCACACTGGGA
GCTAATTCCGGAGATTAAAAAATTACGTGATCGTATTGCACCAAATACGCTCCTAACGATAAACGGAGACATTCCTGACC
GTAAAACTGGGCTGGAACTTGCTGAAAAATACGGCATTGATGGCGTAATGATTGGACGAGGAATCTTTAAAAATCCATTT
GCTTTCGAAAAAGAACCAAGAGAGCATAGCAGTAAAGAACATCTAGATCTTTTAAGACTACAACTTGATCTTCAAGATCA
ATATGCAGAAGTATTACCAAGATCCATAACAGGATTACATCGCTTCTTCAAAATTTATGTAAAAGGCTTCCCTGGGGCTG
CTGAACTACGAAATCAATTGATGAGCACGAAGTCAACGGATGAAGTACGCGCATTGCTTAATAAGTTTGAGGCGAGTGTT
AATGTGGTAGAAGGTAGTGAAACGGTATAA

Upstream 100 bases:

>100_bases
ATAATAGGAAAGCAGTTGATATAGGAAAATCTATACCATCTGCTTTTTGGTTTATTGAGAGTTTGAAATTGTATTCATAG
TGAGAGGATTTGAATTTGAA

Downstream 100 bases:

>100_bases
CTCCCTGAAGTCGAGGGGGTGGATTATATTTATTTAGTTTTAAGTTCTTTTGTAGATTGCAACGGAAAAAACAGTTTTAT
AAATGTATTGAACTAACCAA

Product: dihydrouridine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPMVAHIWGDN
PEYFREMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTH
IFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRALLNKFEASV
NVVEGSETV

Sequences:

>Translated_329_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPMVAHIWGDN
PEYFREMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTH
IFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRALLNKFEASV
NVVEGSETV
>Mature_329_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPMVAHIWGDN
PEYFREMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTH
IFKQDIANLSIHLRTREEMSQVDAHWELIPEIKKLRDRIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQLMSTKSTDEVRALLNKFEASV
NVVEGSETV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=315, Percent_Identity=24.7619047619048, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788462, Length=240, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789660, Length=238, Percent_Identity=28.9915966386555, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI145693211, Length=338, Percent_Identity=24.8520710059172, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=27.2300469483568, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=176, Percent_Identity=31.25, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=312, Percent_Identity=25.9615384615385, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921524, Length=236, Percent_Identity=27.9661016949153, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37299; Mature: 37299

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVR
CCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHC
GRLIFTEDEQPMVAHIWGDNPEYFREMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLI
CEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCCCCEEEECCCCCCCHHCCCCCCCEE
LRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTHIFKQDIANLSIHLRTREEMS
ECCHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHH
QVDAHWELIPEIKKLRDRIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFKNPF
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQL
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MSTKSTDEVRALLNKFEASVNVVEGSETV
HCCCCHHHHHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVR
CCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHC
GRLIFTEDEQPMVAHIWGDNPEYFREMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLI
CEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCCCCEEEECCCCCCCHHCCCCCCCEE
LRPDVAAELIQAAKAGGLPVSVKTRLGFKELSEWEDWLTHIFKQDIANLSIHLRTREEMS
ECCHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCEEEEEECHHHHH
QVDAHWELIPEIKKLRDRIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFKNPF
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFPGAAELRNQL
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
MSTKSTDEVRALLNKFEASVNVVEGSETV
HCCCCHHHHHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]