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The map label for this gene is murF [H]

Identifier: 222094006

GI number: 222094006

Start: 238833

End: 240209

Strand: Direct

Name: murF [H]

Synonym: BCQ_0262

Alternate gene names: 222094006

Gene position: 238833-240209 (Clockwise)

Preceding gene: 222094005

Following gene: 222094007

Centisome position: 4.58

GC content: 36.24

Gene sequence:

>1377_bases
ATGATAAATCGAACGTTAAAACAAGTAGAACAAATGACAAATGGTACGGGATTAGCAGAGCAGTATGAGGGAATTACTAT
ACAAGGAGTGTCTATTGATACGAGAAAAATCGAAAAAGGAAACTTATATGTTCCGATTCAAGGTGAACGTTTCGATGGAC
ATGCTTTTGTAGATAAAGCTGTTGAAAATGGAGCTGTGGCGACATTATGGATGAAAGATGTAGCAAATCCACCTGAGAAT
CTTCCGGTTATTTTTGTAGAAGACACGCTATCAGCATTACAAATGTTAGCAAAAAACTATCGCGACCAATTGGATGTTAA
AGTGATTGGTGTGACAGGTAGTAACGGTAAAACATCTACAAAAGATATTGTAACAAGTCTTCTTGCGACTAAATTCAAAG
TTCAAAAAACAGAAGGAAACTTCAATAATCATATCGGGCTACCTCTTACTATTTTAAACTTAGAAGAAAATACAGAAGTA
GCTGTATTAGAAATGGGAATGTCAAGCCGTGGGGAAATTGAATTCTTATCTAAGTTAGCTCGTCCAAATGCAGCTATCAT
TACGAACATTGGTGAAGCGCATTTAATGGACTTAGGCTCTCGCGAGGCAATTGCTGAAGCGAAATTAGAAATTGTGACAG
GATTACAAGAAGGTGGAGTGTTCGTATATAACGGAGATGAGCCATTATTAACGAATCGTGTTCCTGAAATGAATTTAATA
GCTGAAACAGTTACGTTTGGTGATGCTAGAGCAAATGATTACTATCCAACGACTGTAACGTTACAAGCAACTGGGACACA
CTTTAAAATGAATAGAGAAGAAAACATTTCATTCTATCTACCGGTATTAGGGAAACACAATGTGTATAATACGCTTGCTT
CTATGGCAATTGCGAAACATTTTGGTGTAACGTGGGAAGAGATGAAAGAAGGTTTAGTAACACTTCAAATGACAGGCATG
CGTATGGAAATTGTAAAAACAAATAGTGGATTAACAATTATCAACGATGCTTATAATGCAAGTCCAACAGCTATGGAAGC
AGCATTCCACCTAATGAATGGTTTAGATGGATTCTCTAAAAAAATTGTTGTTCTCGGCGATATGTTAGAGCTTGGAGATC
AAGAAGTACAGTTTCATTATGAGGTAGGTAAATTAATCGATCCAGCAAAAATCTCCTATGTGTTCACATATGGTAAATTA
GGAGCTCAAATTGCTGAAGGAGCGAAAATTAATTTCCCTAACGAACGTGTAAAGGCTTATGATAATAAAGAAGAGTTAGT
AAAAGAGTTGCAAGCAGTAGTCGATGCGAAAGATGTTGTATTAATAAAAGCATCTCGCGGTATGAAATTGGAAGAAGTAA
TTACGATGTTGAAATAA

Upstream 100 bases:

>100_bases
CGTCACGAAGAAAAACAAAAAATTAAATATACAATCTAACAAAAAAAGGAGGAGGCACTATTCGAAGGAATAGTGCTTCC
TCTATGTAAGGAGTGTTTTT

Downstream 100 bases:

>100_bases
TTGGTGTATATAAATAGGGATGATAGCAACGAAAAAAGAAGAAACTTATAATAAGCGCTTACAGTAAATATAAAAAAATA
AAAGTGAAGAAAAAACCTCT

Product: udp-N-acetylmuramoylalanyl-d-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MINRTLKQVEQMTNGTGLAEQYEGITIQGVSIDTRKIEKGNLYVPIQGERFDGHAFVDKAVENGAVATLWMKDVANPPEN
LPVIFVEDTLSALQMLAKNYRDQLDVKVIGVTGSNGKTSTKDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEENTEV
AVLEMGMSSRGEIEFLSKLARPNAAIITNIGEAHLMDLGSREAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPEMNLI
AETVTFGDARANDYYPTTVTLQATGTHFKMNREENISFYLPVLGKHNVYNTLASMAIAKHFGVTWEEMKEGLVTLQMTGM
RMEIVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFSKKIVVLGDMLELGDQEVQFHYEVGKLIDPAKISYVFTYGKL
GAQIAEGAKINFPNERVKAYDNKEELVKELQAVVDAKDVVLIKASRGMKLEEVITMLK

Sequences:

>Translated_458_residues
MINRTLKQVEQMTNGTGLAEQYEGITIQGVSIDTRKIEKGNLYVPIQGERFDGHAFVDKAVENGAVATLWMKDVANPPEN
LPVIFVEDTLSALQMLAKNYRDQLDVKVIGVTGSNGKTSTKDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEENTEV
AVLEMGMSSRGEIEFLSKLARPNAAIITNIGEAHLMDLGSREAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPEMNLI
AETVTFGDARANDYYPTTVTLQATGTHFKMNREENISFYLPVLGKHNVYNTLASMAIAKHFGVTWEEMKEGLVTLQMTGM
RMEIVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFSKKIVVLGDMLELGDQEVQFHYEVGKLIDPAKISYVFTYGKL
GAQIAEGAKINFPNERVKAYDNKEELVKELQAVVDAKDVVLIKASRGMKLEEVITMLK
>Mature_458_residues
MINRTLKQVEQMTNGTGLAEQYEGITIQGVSIDTRKIEKGNLYVPIQGERFDGHAFVDKAVENGAVATLWMKDVANPPEN
LPVIFVEDTLSALQMLAKNYRDQLDVKVIGVTGSNGKTSTKDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEENTEV
AVLEMGMSSRGEIEFLSKLARPNAAIITNIGEAHLMDLGSREAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPEMNLI
AETVTFGDARANDYYPTTVTLQATGTHFKMNREENISFYLPVLGKHNVYNTLASMAIAKHFGVTWEEMKEGLVTLQMTGM
RMEIVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFSKKIVVLGDMLELGDQEVQFHYEVGKLIDPAKISYVFTYGKL
GAQIAEGAKINFPNERVKAYDNKEELVKELQAVVDAKDVVLIKASRGMKLEEVITMLK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=465, Percent_Identity=32.6881720430108, Blast_Score=221, Evalue=9e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50475; Mature: 50475

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRTLKQVEQMTNGTGLAEQYEGITIQGVSIDTRKIEKGNLYVPIQGERFDGHAFVDKA
CCCHHHHHHHHHHCCCCCHHHCCCEEEEEEEEEHEEECCCCEEEEEECCCCCCHHHHHHH
VENGAVATLWMKDVANPPENLPVIFVEDTLSALQMLAKNYRDQLDVKVIGVTGSNGKTST
HCCCCEEEEEEHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCH
KDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEENTEVAVLEMGMSSRGEIEFLSKLA
HHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHC
RPNAAIITNIGEAHLMDLGSREAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPEMNLI
CCCEEEEEECCCHHHHCCCCHHHHHHHHHHHEECCCCCCEEEECCCCCCCCCCCCCCHHE
AETVTFGDARANDYYPTTVTLQATGTHFKMNREENISFYLPVLGKHNVYNTLASMAIAKH
EEEEECCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHH
FGVTWEEMKEGLVTLQMTGMRMEIVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFSK
CCCCHHHHHCCEEEEEECCEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
KIVVLGDMLELGDQEVQFHYEVGKLIDPAKISYVFTYGKLGAQIAEGAKINFPNERVKAY
EEEEEECHHHCCCCEEEEEEECCCCCCCCEEEEEEEECHHCHHHHCCCEECCCHHHHHCC
DNKEELVKELQAVVDAKDVVLIKASRGMKLEEVITMLK
CCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCC
>Mature Secondary Structure
MINRTLKQVEQMTNGTGLAEQYEGITIQGVSIDTRKIEKGNLYVPIQGERFDGHAFVDKA
CCCHHHHHHHHHHCCCCCHHHCCCEEEEEEEEEHEEECCCCEEEEEECCCCCCHHHHHHH
VENGAVATLWMKDVANPPENLPVIFVEDTLSALQMLAKNYRDQLDVKVIGVTGSNGKTST
HCCCCEEEEEEHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCH
KDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEENTEVAVLEMGMSSRGEIEFLSKLA
HHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHC
RPNAAIITNIGEAHLMDLGSREAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPEMNLI
CCCEEEEEECCCHHHHCCCCHHHHHHHHHHHEECCCCCCEEEECCCCCCCCCCCCCCHHE
AETVTFGDARANDYYPTTVTLQATGTHFKMNREENISFYLPVLGKHNVYNTLASMAIAKH
EEEEECCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHH
FGVTWEEMKEGLVTLQMTGMRMEIVKTNSGLTIINDAYNASPTAMEAAFHLMNGLDGFSK
CCCCHHHHHCCEEEEEECCEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
KIVVLGDMLELGDQEVQFHYEVGKLIDPAKISYVFTYGKLGAQIAEGAKINFPNERVKAY
EEEEEECHHHCCCCEEEEEEECCCCCCCCEEEEEEEECHHCHHHHCCCEECCCHHHHHCC
DNKEELVKELQAVVDAKDVVLIKASRGMKLEEVITMLK
CCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]