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The map label for this gene is ddl [H]

Identifier: 222094005

GI number: 222094005

Start: 237686

End: 238771

Strand: Direct

Name: ddl [H]

Synonym: BCQ_0261

Alternate gene names: 222094005

Gene position: 237686-238771 (Clockwise)

Preceding gene: 222094004

Following gene: 222094006

Centisome position: 4.56

GC content: 36.37

Gene sequence:

>1086_bases
GTGACAAAAATTAAATTAGGTTTATTATATGGTGGAAAATCAGCTGAGCATCAAGTTTCGCTACAAACGGCTCTTGCTGC
TATTAAAGCATTAAATCAAGATAAATTCGAGATTCATCCAATTTATATTACAGAACAAGGTCAATGGGTACGCGGTGAGC
GTATTGAAGGTGAAGTAACAGATGTTGAAATTCTAAAAATGAGCGGTGCAGAAAATGCAATTTCTCCGCTATCATTAAGC
ACAGAAATCATTCCATCTGCAAATGCTGAGGAAAATGCTATCGACGTTATTTTCCCATTACTACATGGACCAAATGGTGA
AGATGGAACGGTACAAGGATTATTAGAATTAATGAATATTCCTTATGTAGGAAATGGTGTTCTAGCATCTGCTGCTGGTA
TGGATAAAGTTGTTATGAAAAACATTTTCGCAGAAGCTGGATTAAAACAAGCGAAATATGCATCATTCATTCGTAGCGGA
TGGGAAAAAGATCGTGAAACAGCATATGAAAAAGTAGAAGAAGTATTAGGATACCCTTGCTTCGTAAAACCAGCAAACCT
TGGTTCAAGTGTTGGGATTAATAAGTGTAAAAATCGTGAAGAACTTGAGAATGCATTTGAAGAGGCATTCCAATTTGACC
GTAAAATTATTGTGGAAGAAAATATCGTTGGACGTGAAGTAGAAGTTGGTGTACTAGGTAATGATGAACCGAAATGTTCA
GTTGTAGGTGAAATCGTACCTAAAAAAGACTTCTATGATTATAAGTCGAAATACATCGATGGTGATACAGCGTTAATTAT
TCCAGCTGAAATGACAGAAGAAGAGTCTAATGTTATTAAGAGAGATGCGATTGTTGCATTCCAATCGTTAGATGGTGCTG
GCTTAACGCGAGCTGATTTCTTCTTAACGAAAGATGGAGAAGTATACATTAACGAAGTGAATACGATGCCAGGATTTACG
CCGTTTAGTATGTTCCCTCTACTATGGCAACATACTGGATTACCGTATCCGGAATTAATTGAAGAGCTAATTCGTTTAGC
AATGGAGCGTCACGAAGAAAAACAAAAAATTAAATATACAATCTAA

Upstream 100 bases:

>100_bases
ATTATTTGTGGAAGTTGCATTTTATTTCAAATAGGAACTACCTATTTTTATAAAAAGTGCTAAAATAGAAAAATGAAAAC
AATTGGTTAGGGTGATTTCA

Downstream 100 bases:

>100_bases
CAAAAAAAGGAGGAGGCACTATTCGAAGGAATAGTGCTTCCTCTATGTAAGGAGTGTTTTTATGATAAATCGAACGTTAA
AACAAGTAGAACAAATGACA

Product: D-alanyl-alanine synthetase a

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWVRGERIEGEVTDVEILKMSGAENAISPLSLS
TEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLKQAKYASFIRSG
WEKDRETAYEKVEEVLGYPCFVKPANLGSSVGINKCKNREELENAFEEAFQFDRKIIVEENIVGREVEVGVLGNDEPKCS
VVGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESNVIKRDAIVAFQSLDGAGLTRADFFLTKDGEVYINEVNTMPGFT
PFSMFPLLWQHTGLPYPELIEELIRLAMERHEEKQKIKYTI

Sequences:

>Translated_361_residues
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWVRGERIEGEVTDVEILKMSGAENAISPLSLS
TEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLKQAKYASFIRSG
WEKDRETAYEKVEEVLGYPCFVKPANLGSSVGINKCKNREELENAFEEAFQFDRKIIVEENIVGREVEVGVLGNDEPKCS
VVGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESNVIKRDAIVAFQSLDGAGLTRADFFLTKDGEVYINEVNTMPGFT
PFSMFPLLWQHTGLPYPELIEELIRLAMERHEEKQKIKYTI
>Mature_360_residues
TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWVRGERIEGEVTDVEILKMSGAENAISPLSLST
EIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLKQAKYASFIRSGW
EKDRETAYEKVEEVLGYPCFVKPANLGSSVGINKCKNREELENAFEEAFQFDRKIIVEENIVGREVEVGVLGNDEPKCSV
VGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESNVIKRDAIVAFQSLDGAGLTRADFFLTKDGEVYINEVNTMPGFTP
FSMFPLLWQHTGLPYPELIEELIRLAMERHEEKQKIKYTI

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=375, Percent_Identity=43.2, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1786280, Length=343, Percent_Identity=32.3615160349854, Blast_Score=178, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 40145; Mature: 40014

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWVRGERIEGEVT
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEECCCCCCCCCC
DVEILKMSGAENAISPLSLSTEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNI
CEEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHEEEEEHHCCCCCCCHHHHHHHHHHCC
PYVGNGVLASAAGMDKVVMKNIFAEAGLKQAKYASFIRSGWEKDRETAYEKVEEVLGYPC
CCCCCCHHEHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FVKPANLGSSVGINKCKNREELENAFEEAFQFDRKIIVEENIVGREVEVGVLGNDEPKCS
EEECCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCEE
VVGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESNVIKRDAIVAFQSLDGAGLTRADF
EHHHHCCCCCHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHEEECCCCCCCEEEEE
FLTKDGEVYINEVNTMPGFTPFSMFPLLWQHTGLPYPELIEELIRLAMERHEEKQKIKYT
EEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECC
I
C
>Mature Secondary Structure 
TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWVRGERIEGEVT
CEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEECCCCCCCCCC
DVEILKMSGAENAISPLSLSTEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNI
CEEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHEEEEEHHCCCCCCCHHHHHHHHHHCC
PYVGNGVLASAAGMDKVVMKNIFAEAGLKQAKYASFIRSGWEKDRETAYEKVEEVLGYPC
CCCCCCHHEHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FVKPANLGSSVGINKCKNREELENAFEEAFQFDRKIIVEENIVGREVEVGVLGNDEPKCS
EEECCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCEE
VVGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESNVIKRDAIVAFQSLDGAGLTRADF
EHHHHCCCCCHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHEEECCCCCCCEEEEE
FLTKDGEVYINEVNTMPGFTPFSMFPLLWQHTGLPYPELIEELIRLAMERHEEKQKIKYT
EEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA