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The map label for this gene is oppA [H]

Identifier: 222093991

GI number: 222093991

Start: 222942

End: 224669

Strand: Direct

Name: oppA [H]

Synonym: BCQ_0247

Alternate gene names: 222093991

Gene position: 222942-224669 (Clockwise)

Preceding gene: 222093990

Following gene: 222093992

Centisome position: 4.28

GC content: 34.32

Gene sequence:

>1728_bases
ATGAAGAAAAAGACAAAAAAATGGGCTGGTGTATTTTCGGTATTACTGAGTAGTTCGCTTGTGTTATCTGCTTGTGGGGG
ACAGGAAGATACTGCTTCTACAGAACCGGTTAAACAGCAGGATTTAAAAAATATAAAAGTTGAAAAAATTGCGGCTACAG
ATAAGACGAAAGTACCTGATAAGGCGAAAAATAGAAAAGATACGTTTATTTCTGGTATCTCAAAGCCGGGGGGAGTTTTC
CTACCTTACTTTCAAGAGAACGGTTGGGATGGCAATGTGACATCGGTTATTTTCGCCTCTCTTGTTACAACAGATAAACA
AGGGAAACCTATACCAGATCTTGCCGAGAAATGGGATGTCTCTTCAGACCAATTAACATATACATTTCATTTACGCAAAG
ACTTGAAATTTAGTGATGGATCGCCATTAACTGCTGACGATGTAGCATTTACATTATCACTTCTACATGATAAAGCTTAT
GAAGGTGGCGATGATATTTCACAATATGCAATTAAAGGTGGTAAGGAATATAAAGAAGGAAAAGCGACGTCTATTGAAGG
GATTCAAGTTGTTGATCCACAAACAATTAAAGTTACGACTGAAAAAGTTAACTCACAAGCGCTAATTGCTTTAGGTGGTC
CAGTATTATCAAAAGCTTATTATGGAAAAGAATATAAGCAAAATACTAGTTTAGATTATTTAAAAGAGTTATATGGGAAA
CCGATAGCTGCGGGACCATATAAGTTTGAAAAATATATTCCAGGACAGGAAGTTCGTTTTACAGCAAATGAAAATTATTA
TGCAGGTAAACCAAAAATTCAAAACTTTATTTATAAGATTACTTCACCAGATACAAAATTCCAATTATTCCAAACAGGTG
AGATAGATTATACAGGAGTAGGTTACGGGGATGAAATTTTAGAACAAGTGAAGAGTTTGCAGTTTGCAAATGTTGAAATA
GAGACGGCTTCTTCATTTAGTTATATAAGAATGAATAGTAAAAAACCGTATTTAAAAGATAAGAAAGTTCGTCAAGCTCT
TATTTATGGTTTGGATCGTAAAAAATATGTAGATACAGCTTTCAAAGGATACGGTACAGTAGCTAATGTTCCAATTCATC
CAACATCTTGGGCTTATACAGAAGAGGGGATTAATAAGTACGAATATAACTTAGATAAGGCGAAGAAATTATTAGATGAA
GCTGGATGGAAAGCTGGTTCAGACGGAATTCGTGAAAAAGATGGTCAAAAGCTTAAATTAACGTATTATGGTGCAAGTTC
AGCAAAAGACGGTGATTTGTTTATTCCGATAGCAAAAGAGAACTATAAAGAATTAGGTGTTGAGTTCAATCCTGAATTTA
TGGATTTCAATACGATGCTGTCAAAAGTGAAAAAAGGCGATTATGATTTAGCTTCAGTTGCAACACCAATTACAACAGAT
CCAAGTGAAACTGTAAGTGGTTACTCCTCAGAAGGTGATGATAATACCTTAGGTTATAAAAATGAAAAAGTAGATGCATT
AATTAAAAAAGGAATTGAAACTGTAGATATTGAGAAACGTAAACCAATTTACAAAGAACTGTACAAAGAACTAAGCGATG
ATCCGCCAGTAATTTTATTGAATTATCGCAGAGTCATCACAGCTTACAATGGAAACATTAAAGGGATTGATCCTGAGAAG
TATGACAGTATTAGTTCGAACTCACCAGTATTATCTATTGAGAAATAA

Upstream 100 bases:

>100_bases
AAAACCGACTTTTCCGATTGGCTATAAATGTTGCTGTGGGAGGCATACATAAGGTAATAAGTTCAAAGGGTATCACTATT
AAAAAGTTAGGGTGGGGACA

Downstream 100 bases:

>100_bases
CGAGAAGAATGTATAAGTAGAAGACATTTTTTCATTCGGCTAGTAATATGCTGATTCGAAAAAATGTTCTTCTATTTTAA
GCATAAGATTGGAGAGAAAA

Product: peptide ABC transporter substrate-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MKKKTKKWAGVFSVLLSSSLVLSACGGQEDTASTEPVKQQDLKNIKVEKIAATDKTKVPDKAKNRKDTFISGISKPGGVF
LPYFQENGWDGNVTSVIFASLVTTDKQGKPIPDLAEKWDVSSDQLTYTFHLRKDLKFSDGSPLTADDVAFTLSLLHDKAY
EGGDDISQYAIKGGKEYKEGKATSIEGIQVVDPQTIKVTTEKVNSQALIALGGPVLSKAYYGKEYKQNTSLDYLKELYGK
PIAAGPYKFEKYIPGQEVRFTANENYYAGKPKIQNFIYKITSPDTKFQLFQTGEIDYTGVGYGDEILEQVKSLQFANVEI
ETASSFSYIRMNSKKPYLKDKKVRQALIYGLDRKKYVDTAFKGYGTVANVPIHPTSWAYTEEGINKYEYNLDKAKKLLDE
AGWKAGSDGIREKDGQKLKLTYYGASSAKDGDLFIPIAKENYKELGVEFNPEFMDFNTMLSKVKKGDYDLASVATPITTD
PSETVSGYSSEGDDNTLGYKNEKVDALIKKGIETVDIEKRKPIYKELYKELSDDPPVILLNYRRVITAYNGNIKGIDPEK
YDSISSNSPVLSIEK

Sequences:

>Translated_575_residues
MKKKTKKWAGVFSVLLSSSLVLSACGGQEDTASTEPVKQQDLKNIKVEKIAATDKTKVPDKAKNRKDTFISGISKPGGVF
LPYFQENGWDGNVTSVIFASLVTTDKQGKPIPDLAEKWDVSSDQLTYTFHLRKDLKFSDGSPLTADDVAFTLSLLHDKAY
EGGDDISQYAIKGGKEYKEGKATSIEGIQVVDPQTIKVTTEKVNSQALIALGGPVLSKAYYGKEYKQNTSLDYLKELYGK
PIAAGPYKFEKYIPGQEVRFTANENYYAGKPKIQNFIYKITSPDTKFQLFQTGEIDYTGVGYGDEILEQVKSLQFANVEI
ETASSFSYIRMNSKKPYLKDKKVRQALIYGLDRKKYVDTAFKGYGTVANVPIHPTSWAYTEEGINKYEYNLDKAKKLLDE
AGWKAGSDGIREKDGQKLKLTYYGASSAKDGDLFIPIAKENYKELGVEFNPEFMDFNTMLSKVKKGDYDLASVATPITTD
PSETVSGYSSEGDDNTLGYKNEKVDALIKKGIETVDIEKRKPIYKELYKELSDDPPVILLNYRRVITAYNGNIKGIDPEK
YDSISSNSPVLSIEK
>Mature_575_residues
MKKKTKKWAGVFSVLLSSSLVLSACGGQEDTASTEPVKQQDLKNIKVEKIAATDKTKVPDKAKNRKDTFISGISKPGGVF
LPYFQENGWDGNVTSVIFASLVTTDKQGKPIPDLAEKWDVSSDQLTYTFHLRKDLKFSDGSPLTADDVAFTLSLLHDKAY
EGGDDISQYAIKGGKEYKEGKATSIEGIQVVDPQTIKVTTEKVNSQALIALGGPVLSKAYYGKEYKQNTSLDYLKELYGK
PIAAGPYKFEKYIPGQEVRFTANENYYAGKPKIQNFIYKITSPDTKFQLFQTGEIDYTGVGYGDEILEQVKSLQFANVEI
ETASSFSYIRMNSKKPYLKDKKVRQALIYGLDRKKYVDTAFKGYGTVANVPIHPTSWAYTEEGINKYEYNLDKAKKLLDE
AGWKAGSDGIREKDGQKLKLTYYGASSAKDGDLFIPIAKENYKELGVEFNPEFMDFNTMLSKVKKGDYDLASVATPITTD
PSETVSGYSSEGDDNTLGYKNEKVDALIKKGIETVDIEKRKPIYKELYKELSDDPPVILLNYRRVITAYNGNIKGIDPEK
YDSISSNSPVLSIEK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=472, Percent_Identity=27.1186440677966, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1787495, Length=490, Percent_Identity=24.4897959183673, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789887, Length=498, Percent_Identity=24.0963855421687, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1787052, Length=495, Percent_Identity=24.6464646464646, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789397, Length=482, Percent_Identity=24.2738589211618, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1787762, Length=465, Percent_Identity=25.3763440860215, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787551, Length=464, Percent_Identity=23.0603448275862, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87082063, Length=504, Percent_Identity=21.4285714285714, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081878, Length=473, Percent_Identity=22.1987315010571, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64097; Mature: 64097

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKTKKWAGVFSVLLSSSLVLSACGGQEDTASTEPVKQQDLKNIKVEKIAATDKTKVPD
CCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCC
KAKNRKDTFISGISKPGGVFLPYFQENGWDGNVTSVIFASLVTTDKQGKPIPDLAEKWDV
HHCCCHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCC
SSDQLTYTFHLRKDLKFSDGSPLTADDVAFTLSLLHDKAYEGGDDISQYAIKGGKEYKEG
CCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCC
KATSIEGIQVVDPQTIKVTTEKVNSQALIALGGPVLSKAYYGKEYKQNTSLDYLKELYGK
CCCCCCCEEEECCCEEEEEEECCCCEEEEEECCHHHHHHHCCCHHCCCCCHHHHHHHHCC
PIAAGPYKFEKYIPGQEVRFTANENYYAGKPKIQNFIYKITSPDTKFQLFQTGEIDYTGV
CCCCCCCHHHHCCCCCCEEEEECCCEECCCCHHCEEEEEECCCCCEEEEEECCCEEEECC
GYGDEILEQVKSLQFANVEIETASSFSYIRMNSKKPYLKDKKVRQALIYGLDRKKYVDTA
CCCHHHHHHHHCCEECEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
FKGYGTVANVPIHPTSWAYTEEGINKYEYNLDKAKKLLDEAGWKAGSDGIREKDGQKLKL
HHCCCCEEECCCCCCCCCHHHHCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
TYYGASSAKDGDLFIPIAKENYKELGVEFNPEFMDFNTMLSKVKKGDYDLASVATPITTD
EEECCCCCCCCCEEEEECCCCHHHCCCEECCCEECHHHHHHHHHCCCCCHHHHCCCCCCC
PSETVSGYSSEGDDNTLGYKNEKVDALIKKGIETVDIEKRKPIYKELYKELSDDPPVILL
CHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCEEEE
NYRRVITAYNGNIKGIDPEKYDSISSNSPVLSIEK
ECEEEEEEECCCCCCCCCHHHCCCCCCCCEEEECC
>Mature Secondary Structure
MKKKTKKWAGVFSVLLSSSLVLSACGGQEDTASTEPVKQQDLKNIKVEKIAATDKTKVPD
CCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCC
KAKNRKDTFISGISKPGGVFLPYFQENGWDGNVTSVIFASLVTTDKQGKPIPDLAEKWDV
HHCCCHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCC
SSDQLTYTFHLRKDLKFSDGSPLTADDVAFTLSLLHDKAYEGGDDISQYAIKGGKEYKEG
CCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHCCC
KATSIEGIQVVDPQTIKVTTEKVNSQALIALGGPVLSKAYYGKEYKQNTSLDYLKELYGK
CCCCCCCEEEECCCEEEEEEECCCCEEEEEECCHHHHHHHCCCHHCCCCCHHHHHHHHCC
PIAAGPYKFEKYIPGQEVRFTANENYYAGKPKIQNFIYKITSPDTKFQLFQTGEIDYTGV
CCCCCCCHHHHCCCCCCEEEEECCCEECCCCHHCEEEEEECCCCCEEEEEECCCEEEECC
GYGDEILEQVKSLQFANVEIETASSFSYIRMNSKKPYLKDKKVRQALIYGLDRKKYVDTA
CCCHHHHHHHHCCEECEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
FKGYGTVANVPIHPTSWAYTEEGINKYEYNLDKAKKLLDEAGWKAGSDGIREKDGQKLKL
HHCCCCEEECCCCCCCCCHHHHCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
TYYGASSAKDGDLFIPIAKENYKELGVEFNPEFMDFNTMLSKVKKGDYDLASVATPITTD
EEECCCCCCCCCEEEEECCCCHHHCCCEECCCEECHHHHHHHHHCCCCCHHHHCCCCCCC
PSETVSGYSSEGDDNTLGYKNEKVDALIKKGIETVDIEKRKPIYKELYKELSDDPPVILL
CHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCEEEE
NYRRVITAYNGNIKGIDPEKYDSISSNSPVLSIEK
ECEEEEEEECCCCCCCCCHHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]