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The map label for this gene is appA [H]

Identifier: 222093990

GI number: 222093990

Start: 220999

End: 222699

Strand: Direct

Name: appA [H]

Synonym: BCQ_0246

Alternate gene names: 222093990

Gene position: 220999-222699 (Clockwise)

Preceding gene: 222093988

Following gene: 222093991

Centisome position: 4.24

GC content: 35.86

Gene sequence:

>1701_bases
ATGAAAAATGTTTTTAAAGGAGCAATTGCTACATTTTTGAGTGTATCAGTATTACTAGCCGGATGTGGTCAAGAGGAAGC
GAGTACAAACGAGGCCAGGAACGTCCCGAAAGTAAAAGATGAATTTATTCAGGCAAGCGACAAAGCAAAAAGTCCCGTAA
AAGCAAAAGAAAGAAAAGATACTTTTGTAGTTGGAATGCCGAGTCCTGGAGGGATATTCCTTCCGCACTTTATGGAGAAT
GGATGGGATGGTAATATAACGCAAGCTATCTTTGCACCTCTTGTTGGACTCGATAAGGAAGGAAAACCAATTCCAATTTT
AGCGAAGAAGTGGGATATTTCGGAGGATCAGCTTACATACACGTTCTATTTGAAAGATGGTTTAAAGTTTAGTGATGGAT
CACCACTAACTGCAGATGACGTAGCATTTACATTAACGCTATTACACGATCCAACTTATAGTGGAGCGACTGATATAAGT
CAAACAGCAATAAAGGGTGGGCAAGCGTATAAAGAAGGAAAGGCAACCTTTATTGAGGGAATTCAAGTCATTGATCCGAA
AACAATTACGATTACGACTGAAAAGGTAAATGCTCAAACACTATCATTAATCGGTGGAGAAGTTATATCTAAAGCTTATT
ACGGGAAAGAGTATAAACAAGGGCACCTAGAGTATTTAAAAGAGTTGTATGGAAAACCGATGGGAGCAGGAGCTTATAAG
TTAGATAAATATATTCCTGGTCAAGAGGTTCGATTTGTAGCTAATGAAAATTATTTTGAAGGAAAACCGAAGATTGAGCA
CTTTATTTATAAAATTTCAAAAGGTGATACAAAGCTACAACAATTTCAAGCCGGGGAAGTAGATTATGATGGTTTTACGA
CAAACACGGAGATGATTGAACAGTTAAAGGATTTAGGTTTTGCCAATATTAATGTATATACAGGAAGCTCTTATGGTTAC
ATTAAAATGAATTATAAGAAACCATACTTTAAAGATAAGCGTGTCCGCCAAGCATTTATTTACGGATTAGAGCGTCAAAA
AGTTATTGATACGTATTTCCAAGGTTATGCTTCACTCGTTAATGTACCGATTACGCCAGTTTCTTGGGCGTATACAGAAG
AAGGGATTAATAAATATGAGTATAACTTAGAGAAAGCAAAGAAATTATTGGATGAGGCTGGATGGAAAGCTGGTTCAGAT
GGAATTCGCGAAAAAGATGGCCAAAAATTGAAGGTAAGTTATTTTGCTTCTTCTGCAAGTAAAATAAATGATGTAATGAT
CCCGGTGATGAAAGAGAATTATAAAAAGCTTGGTGTAGAGTTTAACCCAGAATATATGGACTTTAATACGATGATTTCAA
AAGTGATAAAAGGTGATTATGATTTAGCGATGGTATCTACGCCAATGATTGATGATCCGAGTGGAACAATTGAGGAGTTT
GTTTCGACAAGTAAACGAAACTATGATGGGTACCATAATCCGAAAGTAGATGAATTAGCGAAGCAGGCACTAGAAACATT
AGATATTGAAAAACGAAAAGAAATTTATAAAAAGTTGTACCAGGAGTTGAGTGAAGATCCGCCTGTAATCTTCTTAAATA
ATAGTAAAGTTGTGTCTGCACATCATGCACGCATTCAAGGGTTACAAGAAGATAACTATAACGGTATTTTGTTAAGTTTA
CCTAAATTAAAGATAGAATAA

Upstream 100 bases:

>100_bases
TTAATTCATACTATTTTTATAGGAATAATGAATATAGTTGCAGGAAGGAGTCCTATGTTCCTACATCGTTAAAAATTAGG
AAGGTAAAGGTGATAGTGCA

Downstream 100 bases:

>100_bases
TAATTTTTGAAAAATTTTAAAAATATTTATTTAGAAAGAATTGACAAAATATAACGGAAGATATAAGCTAATAACCATAA
AGTCTACCGGAAAGGTCGGA

Product: peptide ABC transporter substrate-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKNVFKGAIATFLSVSVLLAGCGQEEASTNEARNVPKVKDEFIQASDKAKSPVKAKERKDTFVVGMPSPGGIFLPHFMEN
GWDGNITQAIFAPLVGLDKEGKPIPILAKKWDISEDQLTYTFYLKDGLKFSDGSPLTADDVAFTLTLLHDPTYSGATDIS
QTAIKGGQAYKEGKATFIEGIQVIDPKTITITTEKVNAQTLSLIGGEVISKAYYGKEYKQGHLEYLKELYGKPMGAGAYK
LDKYIPGQEVRFVANENYFEGKPKIEHFIYKISKGDTKLQQFQAGEVDYDGFTTNTEMIEQLKDLGFANINVYTGSSYGY
IKMNYKKPYFKDKRVRQAFIYGLERQKVIDTYFQGYASLVNVPITPVSWAYTEEGINKYEYNLEKAKKLLDEAGWKAGSD
GIREKDGQKLKVSYFASSASKINDVMIPVMKENYKKLGVEFNPEYMDFNTMISKVIKGDYDLAMVSTPMIDDPSGTIEEF
VSTSKRNYDGYHNPKVDELAKQALETLDIEKRKEIYKKLYQELSEDPPVIFLNNSKVVSAHHARIQGLQEDNYNGILLSL
PKLKIE

Sequences:

>Translated_566_residues
MKNVFKGAIATFLSVSVLLAGCGQEEASTNEARNVPKVKDEFIQASDKAKSPVKAKERKDTFVVGMPSPGGIFLPHFMEN
GWDGNITQAIFAPLVGLDKEGKPIPILAKKWDISEDQLTYTFYLKDGLKFSDGSPLTADDVAFTLTLLHDPTYSGATDIS
QTAIKGGQAYKEGKATFIEGIQVIDPKTITITTEKVNAQTLSLIGGEVISKAYYGKEYKQGHLEYLKELYGKPMGAGAYK
LDKYIPGQEVRFVANENYFEGKPKIEHFIYKISKGDTKLQQFQAGEVDYDGFTTNTEMIEQLKDLGFANINVYTGSSYGY
IKMNYKKPYFKDKRVRQAFIYGLERQKVIDTYFQGYASLVNVPITPVSWAYTEEGINKYEYNLEKAKKLLDEAGWKAGSD
GIREKDGQKLKVSYFASSASKINDVMIPVMKENYKKLGVEFNPEYMDFNTMISKVIKGDYDLAMVSTPMIDDPSGTIEEF
VSTSKRNYDGYHNPKVDELAKQALETLDIEKRKEIYKKLYQELSEDPPVIFLNNSKVVSAHHARIQGLQEDNYNGILLSL
PKLKIE
>Mature_566_residues
MKNVFKGAIATFLSVSVLLAGCGQEEASTNEARNVPKVKDEFIQASDKAKSPVKAKERKDTFVVGMPSPGGIFLPHFMEN
GWDGNITQAIFAPLVGLDKEGKPIPILAKKWDISEDQLTYTFYLKDGLKFSDGSPLTADDVAFTLTLLHDPTYSGATDIS
QTAIKGGQAYKEGKATFIEGIQVIDPKTITITTEKVNAQTLSLIGGEVISKAYYGKEYKQGHLEYLKELYGKPMGAGAYK
LDKYIPGQEVRFVANENYFEGKPKIEHFIYKISKGDTKLQQFQAGEVDYDGFTTNTEMIEQLKDLGFANINVYTGSSYGY
IKMNYKKPYFKDKRVRQAFIYGLERQKVIDTYFQGYASLVNVPITPVSWAYTEEGINKYEYNLEKAKKLLDEAGWKAGSD
GIREKDGQKLKVSYFASSASKINDVMIPVMKENYKKLGVEFNPEYMDFNTMISKVIKGDYDLAMVSTPMIDDPSGTIEEF
VSTSKRNYDGYHNPKVDELAKQALETLDIEKRKEIYKKLYQELSEDPPVIFLNNSKVVSAHHARIQGLQEDNYNGILLSL
PKLKIE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=468, Percent_Identity=24.7863247863248, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1787495, Length=486, Percent_Identity=24.4855967078189, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787052, Length=492, Percent_Identity=25.609756097561, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789397, Length=472, Percent_Identity=23.5169491525424, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787762, Length=508, Percent_Identity=23.2283464566929, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789887, Length=462, Percent_Identity=22.2943722943723, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787551, Length=528, Percent_Identity=22.1590909090909, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081878, Length=462, Percent_Identity=25.3246753246753, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63597; Mature: 63597

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVFKGAIATFLSVSVLLAGCGQEEASTNEARNVPKVKDEFIQASDKAKSPVKAKERKD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCHHCCCC
TFVVGMPSPGGIFLPHFMENGWDGNITQAIFAPLVGLDKEGKPIPILAKKWDISEDQLTY
EEEEECCCCCCEECHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEE
TFYLKDGLKFSDGSPLTADDVAFTLTLLHDPTYSGATDISQTAIKGGQAYKEGKATFIEG
EEEEECCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCHHHCCCHHHHCC
IQVIDPKTITITTEKVNAQTLSLIGGEVISKAYYGKEYKQGHLEYLKELYGKPMGAGAYK
EEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHH
LDKYIPGQEVRFVANENYFEGKPKIEHFIYKISKGDTKLQQFQAGEVDYDGFTTNTEMIE
HHHCCCCCCEEEEECCCCCCCCCHHHHHEEEECCCCHHHHHHCCCCCCCCCCCCCHHHHH
QLKDLGFANINVYTGSSYGYIKMNYKKPYFKDKRVRQAFIYGLERQKVIDTYFQGYASLV
HHHHCCCEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
NVPITPVSWAYTEEGINKYEYNLEKAKKLLDEAGWKAGSDGIREKDGQKLKVSYFASSAS
CCCCCCCEEHHHHHCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHH
KINDVMIPVMKENYKKLGVEFNPEYMDFNTMISKVIKGDYDLAMVSTPMIDDPSGTIEEF
HHHHHEEHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHH
VSTSKRNYDGYHNPKVDELAKQALETLDIEKRKEIYKKLYQELSEDPPVIFLNNSKVVSA
HHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEE
HHARIQGLQEDNYNGILLSLPKLKIE
HHHHHCCCCCCCCCEEEEECCCEECC
>Mature Secondary Structure
MKNVFKGAIATFLSVSVLLAGCGQEEASTNEARNVPKVKDEFIQASDKAKSPVKAKERKD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCHHCCCC
TFVVGMPSPGGIFLPHFMENGWDGNITQAIFAPLVGLDKEGKPIPILAKKWDISEDQLTY
EEEEECCCCCCEECHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEE
TFYLKDGLKFSDGSPLTADDVAFTLTLLHDPTYSGATDISQTAIKGGQAYKEGKATFIEG
EEEEECCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCHHHCCCHHHHCC
IQVIDPKTITITTEKVNAQTLSLIGGEVISKAYYGKEYKQGHLEYLKELYGKPMGAGAYK
EEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHH
LDKYIPGQEVRFVANENYFEGKPKIEHFIYKISKGDTKLQQFQAGEVDYDGFTTNTEMIE
HHHCCCCCCEEEEECCCCCCCCCHHHHHEEEECCCCHHHHHHCCCCCCCCCCCCCHHHHH
QLKDLGFANINVYTGSSYGYIKMNYKKPYFKDKRVRQAFIYGLERQKVIDTYFQGYASLV
HHHHCCCEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
NVPITPVSWAYTEEGINKYEYNLEKAKKLLDEAGWKAGSDGIREKDGQKLKVSYFASSAS
CCCCCCCEEHHHHHCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHH
KINDVMIPVMKENYKKLGVEFNPEYMDFNTMISKVIKGDYDLAMVSTPMIDDPSGTIEEF
HHHHHEEHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHH
VSTSKRNYDGYHNPKVDELAKQALETLDIEKRKEIYKKLYQELSEDPPVIFLNNSKVVSA
HHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEE
HHARIQGLQEDNYNGILLSLPKLKIE
HHHHHCCCCCCCCCEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]